LOCUS       Exported                9390 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T7neg-720-951-proD.
ACCESSION   urn.local...3q9-d2b6ptk
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4914..5058
     misc_feature    1..861
                     /standard_name="p15A origin"
                     /label=p15A origin
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(260..282)
                     /standard_name="TRB839"
                     /label=TRB839
                     /note="Geneious type: PCR_primer"
     misc_feature    320..342
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1256"
     misc_feature    320..342
                     /label=1256
                     /note="1256"
     misc_feature    389..409
                     /standard_name="TRB840"
                     /label=TRB840
                     /note="Geneious type: PCR_primer"
     misc_feature    944..970
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1257"
     misc_feature    944..970
                     /label=1257
                     /note="1257"
     misc_feature    1280..1315
     misc_feature    complement(1280..1309)
                     /label=MSP369
                     /note="MSP369"
     misc_feature    1287..1315
                     /label=MSP370
                     /note="MSP370"
     modified_base   1301..1315
                     /label=USER junction
                     /note="USER junction"
     terminator      1316..1360
                     /note="rrnB1 transcriptional terminator"
     misc_feature    1361..1367
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     misc_feature    complement(1362..1400)
                     /standard_name="TRB302"
                     /label=TRB302
                     /note="Geneious type: PCR_primer"
     modified_base   1368..1382
                     /label=USER junction
                     /note="USER junction"
     misc_feature    1375..1381
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    1381..1404
     misc_feature    1383..1425
                     /standard_name="TRB303"
                     /label=TRB303
                     /note="Geneious type: PCR_primer"
     promoter        1385..1404
                     /note="T7 promoter"
     misc_feature    1385..1400
                     /standard_name="AATTAACCCTCACTAA"
                     /label=AATTAACCCTCACTAA
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1390..1413
     terminator      1412..1557
                     /standard_name="T1 terminator"
                     /label=T1 terminator
     misc_feature    complement(1541..1557)
     misc_feature    complement(1545..1566)
                     /standard_name="TRB523"
                     /label=TRB523
                     /note="Geneious type: PCR_primer"
     misc_feature    1557..1609
                     /standard_name="TRB720"
                     /label=TRB720
                     /note="Geneious type: PCR_primer"
     misc_feature    1557
     misc_feature    1558..1565
                     /label=SbfI
                     /note="SbfI"
     misc_feature    1565..1570
                     /label=BsgI
                     /note="BsgI"
     RBS             1572..1583
                     /standard_name="sd4"
                     /label=sd4
                     /note="RBS sd8"
     misc_feature    1575..1578
                     /standard_name="GGAAA"
                     /label=GGAAA
                     /note="Geneious type: Editing History Replacement"
     CDS             1584..2678
                     /standard_name="P3NEG"
                     /label=P3NEG
     misc_feature    1616
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1619
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     exon            1638..1868
                     /note="N1 Domain"
     exon            1926..2318
                     /note="N2 Domain"
     misc_feature    1984..2013
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD705"
     exon            2430..2675
                     /note="CT Domain (342 - 424)"
     misc_feature    2534..2567
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd706"
     misc_feature    2546..2591
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(2649..2678)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP64"
     misc_feature    2649..2678
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP356"
     CDS             2678..4801
                     /label=xluxAB
                     /note="xluxAB"
     sig_peptide     3761..3820
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(4112..4142)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    4112..4142
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     misc_feature    4135..4163
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="708"
     modified_base   4802..4818
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4802..4818)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    4804..4811
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     misc_feature    4819..4825
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(4826..4867)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    4866..4897
     misc_feature    complement(4866..4895)
                     /label=MSP368
                     /note="MSP368"
     modified_base   4868..4880
                     /label=USER junction
                     /note="USER junction"
     misc_feature    4869..4897
                     /label=MSP367
                     /note="MSP367"
     misc_feature    complement(4876..4907)
                     /label=MSP420
                     /note="MSP420"
     misc_feature    4887
                     /standard_name="T"
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4888..4922
                     /label=MSP419
                     /note="MSP419"
     misc_feature    4898..5058
     primer_bind     4898..4928
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(4898..4913)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(4898..4913)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    4898..4913
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     4940..4991
                     /label=AB365
                     /note="AB365"
     promoter        4963..5003
                     /standard_name="ProD"
                     /label=ProD promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(4968..4991)
     -35_signal      4969..4974
                     /label=-35
                     /note="-35"
     misc_feature    4973..5017
     primer_bind     complement(4975..5026)
                     /label=AB366
                     /note="AB366"
     -10_signal      4992..4997
                     /label=-10
                     /note="-10"
     prim_transcript 5004
                     /label=+1
                     /note="+1"
     primer_bind     complement(5011..5058)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(5023..5051)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(5025..5066)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    5036..5062
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   5036..5058
     primer_bind     5036..5058
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    5047..5083
                     /label=MSP381
                     /note="MSP381"
     RBS             5070..5083
                     /standard_name="sd8"
                     /label=sd8
     CDS             5084..5533
                     /standard_name="lysozyme"
                     /label=lysozyme
     misc_feature    5084..5086
                     /label=MSP381
                     /note="MSP381"
     misc_feature    5191..5216
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    5191..5216
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(5513..5533)
                     /label=MSP912
                     /note="MSP912"
     misc_feature    5524..8036
                     /standard_name="TTCTGACCGTGGTAGCGGCGGTGGTGCGAGTGGTGGCGCGCT 
                     GGAGGTCCTGTTCCAGGGCCCGGGCGGTAGCGCAGGCAGTGGAGCGGGCGGTAACACG 
                     ATTAACATCGCTAAGAACGACTTCTCTGACATCGAACTGGCTGCTATCCCGTTCAACA 
                     CTCTGGCTGACCATTACGGTGAGCGTTTAGCTCGCGAACAGTTGGCCCTTGAGCATGA 
                     GTCTTACGAGATGGGTGAAGCACGCTTCCGCAAGATGTTTGAGCGTCAACTTAAAGCT 
                     GGTGAGGTTGCGGATAACGCTGCCGCCAAGCCTCTCATCACTACCCTACTCCCTAAGA 
                     TGATTGCACGCATCAACGACTGGTTTGAGGAAGTGAAAGCTAAGCTCGGCAGGCGCCC 
                     GACAGCCTTCCAGTTCCTGCAAGAAATCAAGCCGGAAGCCGTAGCGTACATCACCATT 
                     AAGACCACTCTGGCTTGCCTAACCAGTGCTGACAATACAACCGTTCAGGCTGTAGCAA 
                     GCGCAATCGGTCGGGCCATTGAGGACGAGGCTCGCTTCGGTCGTATCCGTGACCTTGA 
                     AGCTAAGCACTTCAAGAAAAACGTTGAGGAACAACTCAACAAGCGCGTAGGGCACGTC 
                     TACAAGAAAGCATTTATGCAAGTTGTCGAGGCTGACATGCTCTCTAAGGGTCTACTCG 
                     GTGGCGAGGCGTGGTCTTCGTGGTATAAGAAAGACTCTATTCATGTAGGAGTACGCTG 
                     CATCGAGATGCTCATTAAGTCAACTGGAATGGTTAGCTTACACCGCCAAAATGCTGGC 
                     GTAGTAGGTCAAGACTCTGAGACTATCGAACTCGCACCTGAATACGCTGAGGCTATCG 
                     CAACCCGTGCAGGTGCGCTGGCTGGCATCTCTCCGATGTTCCAACCTTGCGTAGTTCC 
                     TCCTAAGCCGTGGACTGGCATTACTGGTGGTGGCTATTGGGCTAACGGTCGTCGTCCT 
                     CTGGCGCTGGTGCGTACTCACAGTAAGAAAGCACTGATGCGCTACGAAGACGTTTACA 
                     TGCCTGAGGTGTACAAAGCGATTAACATTGCGCAAAACACCGCATGGAAAATCAACAA 
                     GAAAGTCCTAGCGGTCGCCAACGTAATCACCAAGTGGAAGCATTGTCCGGTCGAGGAC 
                     ATCCCTGCGATTGAGCGTGAAGAACTCCCGATGAAACCGGAAGACATCGACATGAATC 
                     CTGAGGCTCTCACCGCGTGGAAACGTGCTGCCGCTGCTGTGTACCGCAAGGACAAGGC 
                     TCGCAAGTCTCGCCGTATCAGACTTGAGTTCATGCTTGAGCAAGCCAATAAGTTTGCT 
                     AACCATAAGGCCATCTGGTTCCCTTACAACATGGACTGGCGCGGTCGTGTTTACGCTG 
                     TGTCAATGTTCAACCCGCAAGGTAACGATATGACCAAAGGACTGCTTACGCTGGCGAA 
                     AGGTAAACCAATCGGTAAGGAAGGTTACTACTGGCTGAAAATCCACGGTGCAAACTGT 
                     GCGGGTGTCGATAAGGTTCCGTTCCCTGAGCGCATCAAGTTCATTGAGGAAAACCACG 
                     AGAACATCATGGCTTGCGCTAAGTCTCCACTGGAGAACACTTGGTGGGCTGAGCAAGA 
                     TTCTCCGTTCTGCTTCCTTGCGTTCTGCTTTGAGTACGCTGGGGTACAGCACCACGGC 
                     CTGAGCTATAACTGCTCCCTTCCGCTGGCGTTTGACGGGTCTTGCTCTGTCATCCAGC 
                     ACTTCTCCGCGATGCTCCGAGATGAGGTAGGTGGTCGCGCGGTTAACTTGCTTCCTAG 
                     TGAAACCGTTCAGGACATCTACGGGATTGTTGCTAAGAAAGTCAACGAGATTCTACAA 
                     GCAGACGCAATCAATGGGACCGATAACGAAGTAGTTACCGTGACCGATGAGAACACTG 
                     GTGAAATCTCTGAGAAAGTCAAGCTGGGCACTAAGGCACTGGCTGGTCAATGGCTGGC 
                     TTACGGTGTTACTCGCAGTGTGACTAAGCGTTCAGTCATGACGCTGGCTTACGGGTCC 
                     AAAGAGTTCGGCTTCCGTCAACAAGTGCTGGACGATACCATTCAGCCAGCTATTGATT 
                     CCGGCAAGGGTCTGATGTTCACCCAGCCGAATCAGGCTGCTAGATACATGGCTAAGCT 
                     GATTTGGGAATCTGTGAGCGTGACGGTGGTAGCTGCGGTTGAAGCAATGAACTGGCTT 
                     AAGTCTGCTGCTAAGCTGCTGGCTGCTGAGGTCAAAGATAAGAAGACTGGAGAGATTC 
                     TTCGCAAGCGTTGCGCTGTGCATTGGATAACTCCTGATGGTTTCCCTGTGTGGCAGGA 
                     ATACAAGAAGCCTATTCAGACGCGCTTGGACCTGATGTTCCTCGGTCAGTTCCGCTTA 
                     CAGCTTACCATTAACACCAACAAAGATAGCGAGATTGATGCACACAAACAGGAGG"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5534..5713
                     /label=vamp1_60aa
                     /note="vamp1_60aa"
                     /note="Geneious type: CDS"
     CDS             5714..8362
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
     misc_feature    5714..5738
                     /label=MSP913
                     /note="MSP913"
     misc_feature    5746..5765
                     /standard_name="TRB137"
                     /label=TRB137
                     /note="Geneious type: PCR_primer"
     misc_feature    5968..5994
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    5968..5994
                     /label=1253
                     /note="1253"
     misc_feature    6021..6040
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    6021..6040
                     /label=1259
                     /note="1259"
     misc_feature    6150..6183
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    6250..6269
                     /standard_name="TRB138"
                     /label=TRB138
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(6340..6362)
                     /standard_name="bcd1175"
                     /label=bcd1175
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    complement(6344..6365)
                     /standard_name="TRB211"
                     /label=TRB211
                     /note="Geneious type: PCR_primer"
     misc_feature    6347..6364
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    6417..6446
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    6501..6529
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(6664..6695)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    6765..6786
                     /standard_name="BCD1260 (TRB139)"
                     /label=BCD1260 (TRB139)
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    6765..6786
                     /label=1260
                     /note="1260"
     misc_feature    7016..7045
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    7027..7061
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    7096..7114
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    7280..7299
                     /standard_name="TRB140"
                     /label=TRB140
                     /note="Geneious type: PCR_primer"
     misc_feature    7399..7426
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    7472..7494
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    7472..7494
                     /label=1261
                     /note="1261"
     misc_feature    complement(7472..7494)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    complement(7597..7624)
                     /label=MSP859
                     /note="MSP859"
     misc_feature    complement(7613..7661)
     misc_feature    7613..7639
                     /label=MSP860
                     /note="MSP860"
     misc_feature    7616..7645
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    7679..7699
                     /label=MSP63
                     /note="MSP63"
     misc_feature    7700..7720
                     /label=MSP62
                     /note="MSP62"
     misc_feature    7786..7805
                     /standard_name="TRB141"
                     /label=TRB141
                     /note="Geneious type: PCR_primer"
     misc_feature    7818..7836
                     /standard_name="TRB141a"
                     /label=TRB141a
                     /note="Geneious type: PCR_primer"
     misc_feature    8042..8064
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    8042..8064
                     /label=1262
                     /note="1262"
     misc_feature    8280..8300
                     /standard_name="TRB142"
                     /label=TRB142
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(8341..8378)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    8343..8362
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    8368..8392
                     /standard_name="TRB300"
                     /label=TRB300
                     /note="Geneious type: PCR_primer"
     CDS             8377..8559
                     /standard_name="III fragment"
                     /label=III fragment
     promoter        8432..8459
                     /standard_name="internal promoter"
                     /label=internal promoter
                     /note="Geneious type: Promoter"
     misc_feature    8478..8508
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD616"
     misc_feature    complement(8478..8508)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD617"
     misc_feature    8478..8508
                     /label=BCD616
                     /note="BCD616"
     misc_feature    8478..8508
                     /label=BCD617
                     /note="BCD617"
     misc_feature    complement(8530..8559)
                     /label=MSP64
                     /note="MSP64"
     CDS             8562..8588
                     /standard_name="VI fragment"
                     /label=VI fragment
     misc_feature    8576..8595
                     /label=1311
                     /note="1311"
     misc_feature    8596..8616
                     /label=BCD1310
                     /note="BCD1310"
     CDS             8602..9390
                     /standard_name="aadA"
                     /label=aadA
     misc_feature    8819..8848
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1251"
     misc_feature    8819..8848
                     /label=1251
                     /note="1251"
     misc_feature    8941..8963
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1255"
     misc_feature    8941..8963
                     /label=1255
                     /note="1255"
     misc_feature    complement(9018..9047)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1254"
     misc_feature    complement(9018..9047)
                     /label=1254
                     /note="1254"
ORIGIN
        1 acggatcgct tcatgtggca ggagaaaaaa gactgcaccg gtgcgtcagc agaatatgtg
       61 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
      121 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
      181 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
      241 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      361 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
      421 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
      481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
      541 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
      601 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
      661 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
      721 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg
      781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1081 cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc ggggatcgac
     1141 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     1201 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc
     1261 aagtcagcgt aatgctctgc cagtgttaca accaattaac aagaggacat ccggtcaaat
     1321 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt gctgaggaga cttagggacc
     1381 ctactaatac gactcactat agggagaaag aaggagcgac attgctccgt gtattcactc
     1441 gttggaatga atacacagtg cagtgtttat tctgttattt atgccaaaaa taaaggccac
     1501 tatcaggcag ctttgttgtt ctgtttacca agttcaggag gtaactcata agaaagacct
     1561 gcaggtgcag taaaaaggaa aaaatgaaaa aattattatt cgcaattcct ttagttgttc
     1621 ctttctattc tcactccgct gaaactgttc atcaccatca ccatcacgct gaaactgttg
     1681 aaagttgttt agcaaaaccc catacagaaa attcatttac taacgtctgg aaagacgaca
     1741 aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca ggcgttgtag
     1801 tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg cttgctatcc
     1861 ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt tctgagggtg
     1921 gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact tatatcaacc
     1981 ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct aatccttctc
     2041 ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc cgaaataggc
     2101 agggggcatt aactgtttat acgggcactg ttactcaagg cactgacccc gttaaaactt
     2161 attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg aacggtaaat
     2221 tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt gaatatcaag
     2281 gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct ggtggtggtt
     2341 ctggtggcgg ctctgagggt ggtggctctg agggtggcgg ttctgagggt ggcggctctg
     2401 agggaggcgg ttccggtggt ggctcttccc aaatggctca agtcggtgac ggtgataatt
     2461 cacctttaat gaataatttc cgtcaatatt taccttccct ccctcaatcg gttgaatgtc
     2521 gcccttttgt ctttggcgct ggtaaacctt acgagttcag tatcgactgc gataagatca
     2581 acctgttccg cggtgtcttt gcgtttcttt tatatgttgc cacctttatg tatgtatttt
     2641 ctacgtttgc taacatactg cgtaataagg agtcttaatg aaatttggaa actttttgct
     2701 tacataccaa cctccccaat tttcccaaac agaggtaatg aaacgtttgg ttaaattagg
     2761 tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga
     2821 gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa
     2881 attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga
     2941 agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg
     3001 gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc
     3061 ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa
     3121 tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc
     3181 accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct
     3241 accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta
     3301 taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata
     3361 tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg
     3421 gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag
     3481 aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac
     3541 taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat
     3601 tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga
     3661 agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat
     3721 gccatttctt aaagaaaaac aacgttcgct attatattat ggcggtggcg gtagcggcgg
     3781 tggcggtagc ggcggtggcg gtagcggcgg tggcggtagc aaatttggat tgttcttcct
     3841 taacttcatc aattcaacaa ctgttcaaga acagagtata gttcgcatgc aggaaataac
     3901 ggagtatgtt gataagttga attttgaaca gattttagtg tatgaaaatc atttttcaga
     3961 taatggtgtt gtcggcgctc ctctgactgt ttctggtttt ctgctcggtt taacagagaa
     4021 aattaaaatt ggttcattaa atcacatcat tacaactcat catcctgtcc gcatagcgga
     4081 ggaagcttgc ttattggatc agttaagtga agggagattt attttagggt ttagtgattg
     4141 cgaaaaaaaa gatgaaatgc atttttttaa tcgcccggtt gaatatcaac agcaactatt
     4201 tgaagagtgt tatgaaatca ttaacgatgc tttaacaaca ggctattgta atccagataa
     4261 cgatttttat agcttcccta aaatatctgt aaatccccat gcttatacgc caggcggacc
     4321 tcggaaatat gtaacagcaa ccagtcatca tattgttgag tgggcggcca aaaaaggtat
     4381 tcctctcatc tttaagtggg atgattctaa tgatgttaga tatgaatatg ctgaaagata
     4441 taaagccgtt gcggataaat atgacgttga cctatcagag atagaccatc agttaatgat
     4501 attagttaac tataacgaag atagtaataa agctaaacaa gagacgcgtg catttattag
     4561 tgattatgtt cttgaaatgc accctaatga aaatttcgaa aataaacttg aagaaataat
     4621 tgcagaaaac gctgtcggaa attatacgga gtgtataact gcggctaagt tggcaattga
     4681 aaagtgtggt gcgaaaagtg tattgctgtc ctttgaacca atgaatgatt tgatgagcca
     4741 aaaaaatgta atcaatattg ttgatgataa tattaagaag taccacacgg aatataccta
     4801 aacttaatta acggcactcc tcagcaaata taatgaccct cttgataacc caagagggca
     4861 ttttttaatg cccatggcgt caattccgat ttccggtatt ctagagcaca gctaacacca
     4921 cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg
     4981 cataaggctc gtataatata ttcagggaga ccacaacggt ttccctctac aaataatttt
     5041 gtttaacttt tactagagtt ccttcctcga aaggaaaaaa aaaatggctc gtgtacagtt
     5101 taaacaacgt gaatctactg acgcaatctt tgttcactgc tcggctacca agccaagtca
     5161 gaatgttggt gtccgtgaga ttcgccagtg gcacaaagag cagggttggc tcgatgtggg
     5221 ataccacttt atcatcaagc gagacggtac tgtggaggca ggacgagatg agatggctgt
     5281 aggctctcac gctaagggtt acaaccacaa ctctatcggc gtctgccttg ttggtggtat
     5341 cgacgataaa ggtaagttcg acgctaactt tacgccagcc caaatgcaat cccttcgctc
     5401 actgcttgtc acactgctgg ctaagtacga aggcgctgtg cttcgcgccc atcatgaggt
     5461 ggcgccgaag gcttcccctt cgttcgacct taagcgttgg tgggagaaga acgaactggt
     5521 cacatctgac cgtacctcta accgtcgtct gcagcagacc caggctcagg ttgaagaagt
     5581 tgttgacatc atccgtgtta acgttgacaa agttctggaa cgtgaccaga aactgtctga
     5641 actggacgac cgtgctgacg ctctgcaggc tggtgcttct cagttcgaat cttctgctgc
     5701 taaactgaaa cgtaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc
     5761 tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt
     5821 ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg
     5881 tcaacttaaa gctggtgagg ttgcggataa cgctgccgct aagcctctca tcactaccct
     5941 actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg
     6001 caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat
     6061 caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt
     6121 agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct
     6181 tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt
     6241 ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg
     6301 tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat
     6361 cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt
     6421 aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg
     6481 tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc
     6541 gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt
     6601 gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta
     6661 caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt
     6721 cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg
     6781 tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg
     6841 gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag
     6901 ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc
     6961 ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa
     7021 cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta
     7081 ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga
     7141 gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact
     7201 ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga
     7261 gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga
     7321 cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg
     7381 cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa
     7441 agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt
     7501 gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc
     7561 tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct
     7621 ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc
     7681 agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat
     7741 ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa
     7801 ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga
     7861 gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca
     7921 ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt
     7981 acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg
     8041 tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg
     8101 ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat
     8161 tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga
     8221 gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca
     8281 attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga
     8341 gtcggacttc gcgttcgcgt aatggagatt ttcaacatgc tccctcaatc ggttgaatgt
     8401 cgcccttttg tctttggcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata
     8461 aacttattcc gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt
     8521 tctacgtttg ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta
     8581 ttccgtagaa aaaggaagag tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     8641 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8701 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8761 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8821 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8881 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8941 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     9001 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     9061 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     9121 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     9181 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     9241 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     9301 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     9361 aaaggcgaga tcaccaaggt agtcggcaaa
//
