LOCUS       Exported                9392 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T7neg-721-951-proD.
ACCESSION   urn.local...3qa-d2b6ptk
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1572..1585
     source          4916..5060
     misc_feature    1..861
                     /standard_name="p15A origin"
                     /label=p15A origin
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(260..282)
                     /standard_name="TRB839"
                     /label=TRB839
                     /note="Geneious type: PCR_primer"
     misc_feature    320..342
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1256"
     misc_feature    320..342
                     /label=1256
                     /note="1256"
     misc_feature    389..409
                     /standard_name="TRB840"
                     /label=TRB840
                     /note="Geneious type: PCR_primer"
     misc_feature    944..970
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1257"
     misc_feature    944..970
                     /label=1257
                     /note="1257"
     misc_feature    1280..1315
     misc_feature    complement(1280..1309)
                     /label=MSP369
                     /note="MSP369"
     misc_feature    1287..1315
                     /label=MSP370
                     /note="MSP370"
     modified_base   1301..1315
                     /label=USER junction
                     /note="USER junction"
     terminator      1316..1360
                     /note="rrnB1 transcriptional terminator"
     misc_feature    1361..1367
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     misc_feature    complement(1362..1400)
                     /standard_name="TRB302"
                     /label=TRB302
                     /note="Geneious type: PCR_primer"
     modified_base   1368..1382
                     /label=USER junction
                     /note="USER junction"
     misc_feature    1375..1381
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    1381..1404
     misc_feature    1383..1425
                     /standard_name="TRB303"
                     /label=TRB303
                     /note="Geneious type: PCR_primer"
     promoter        1385..1404
                     /note="T7 promoter"
     misc_feature    1385..1400
                     /standard_name="AATTAACCCTCACTAA"
                     /label=AATTAACCCTCACTAA
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1390..1413
     terminator      1412..1557
                     /standard_name="T1 terminator"
                     /label=T1 terminator
     misc_feature    complement(1541..1557)
     misc_feature    complement(1545..1566)
                     /standard_name="TRB523"
                     /label=TRB523
                     /note="Geneious type: PCR_primer"
     misc_feature    1557..1611
                     /standard_name="TRB721"
                     /label=TRB721
                     /note="Geneious type: PCR_primer"
     misc_feature    1557
     misc_feature    1558..1565
                     /label=SbfI
                     /note="SbfI"
     misc_feature    1565..1570
                     /label=BsgI
                     /note="BsgI"
     RBS             1572..1585
                     /standard_name="sd2"
                     /label=sd2
     misc_feature    1575..1582
                     /standard_name="GGAAAAA"
                     /label=GGAAAAA
                     /note="Geneious type: Editing History Replacement"
     CDS             1586..2680
                     /standard_name="P3NEG"
                     /label=P3NEG
     misc_feature    1618
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1621
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     exon            1640..1870
                     /note="N1 Domain"
     exon            1928..2320
                     /note="N2 Domain"
     misc_feature    1986..2015
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD705"
     exon            2432..2677
                     /note="CT Domain (342 - 424)"
     misc_feature    2536..2569
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd706"
     misc_feature    2548..2593
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(2651..2680)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP64"
     misc_feature    2651..2680
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP356"
     CDS             2680..4803
                     /label=xluxAB
                     /note="xluxAB"
     sig_peptide     3763..3822
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(4114..4144)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    4114..4144
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     misc_feature    4137..4165
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="708"
     modified_base   4804..4820
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4804..4820)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    4806..4813
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     misc_feature    4821..4827
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(4828..4869)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    4868..4899
     misc_feature    complement(4868..4897)
                     /label=MSP368
                     /note="MSP368"
     modified_base   4870..4882
                     /label=USER junction
                     /note="USER junction"
     misc_feature    4871..4899
                     /label=MSP367
                     /note="MSP367"
     misc_feature    complement(4878..4909)
                     /label=MSP420
                     /note="MSP420"
     misc_feature    4889
                     /standard_name="T"
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4890..4924
                     /label=MSP419
                     /note="MSP419"
     misc_feature    4900..5060
     primer_bind     4900..4930
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(4900..4915)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(4900..4915)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    4900..4915
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     4942..4993
                     /label=AB365
                     /note="AB365"
     promoter        4965..5005
                     /standard_name="ProD"
                     /label=ProD promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(4970..4993)
     -35_signal      4971..4976
                     /label=-35
                     /note="-35"
     misc_feature    4975..5019
     primer_bind     complement(4977..5028)
                     /label=AB366
                     /note="AB366"
     -10_signal      4994..4999
                     /label=-10
                     /note="-10"
     prim_transcript 5006
                     /label=+1
                     /note="+1"
     primer_bind     complement(5013..5060)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(5025..5053)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(5027..5068)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    5038..5064
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   5038..5060
     primer_bind     5038..5060
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    5049..5085
                     /label=MSP381
                     /note="MSP381"
     RBS             5072..5085
                     /standard_name="sd8"
                     /label=sd8
     CDS             5086..5535
                     /standard_name="lysozyme"
                     /label=lysozyme
     misc_feature    5086..5088
                     /label=MSP381
                     /note="MSP381"
     misc_feature    5193..5218
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    5193..5218
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(5515..5535)
                     /label=MSP912
                     /note="MSP912"
     misc_feature    5526..8038
                     /standard_name="TTCTGACCGTGGTAGCGGCGGTGGTGCGAGTGGTGGCGCGCT 
                     GGAGGTCCTGTTCCAGGGCCCGGGCGGTAGCGCAGGCAGTGGAGCGGGCGGTAACACG 
                     ATTAACATCGCTAAGAACGACTTCTCTGACATCGAACTGGCTGCTATCCCGTTCAACA 
                     CTCTGGCTGACCATTACGGTGAGCGTTTAGCTCGCGAACAGTTGGCCCTTGAGCATGA 
                     GTCTTACGAGATGGGTGAAGCACGCTTCCGCAAGATGTTTGAGCGTCAACTTAAAGCT 
                     GGTGAGGTTGCGGATAACGCTGCCGCCAAGCCTCTCATCACTACCCTACTCCCTAAGA 
                     TGATTGCACGCATCAACGACTGGTTTGAGGAAGTGAAAGCTAAGCTCGGCAGGCGCCC 
                     GACAGCCTTCCAGTTCCTGCAAGAAATCAAGCCGGAAGCCGTAGCGTACATCACCATT 
                     AAGACCACTCTGGCTTGCCTAACCAGTGCTGACAATACAACCGTTCAGGCTGTAGCAA 
                     GCGCAATCGGTCGGGCCATTGAGGACGAGGCTCGCTTCGGTCGTATCCGTGACCTTGA 
                     AGCTAAGCACTTCAAGAAAAACGTTGAGGAACAACTCAACAAGCGCGTAGGGCACGTC 
                     TACAAGAAAGCATTTATGCAAGTTGTCGAGGCTGACATGCTCTCTAAGGGTCTACTCG 
                     GTGGCGAGGCGTGGTCTTCGTGGTATAAGAAAGACTCTATTCATGTAGGAGTACGCTG 
                     CATCGAGATGCTCATTAAGTCAACTGGAATGGTTAGCTTACACCGCCAAAATGCTGGC 
                     GTAGTAGGTCAAGACTCTGAGACTATCGAACTCGCACCTGAATACGCTGAGGCTATCG 
                     CAACCCGTGCAGGTGCGCTGGCTGGCATCTCTCCGATGTTCCAACCTTGCGTAGTTCC 
                     TCCTAAGCCGTGGACTGGCATTACTGGTGGTGGCTATTGGGCTAACGGTCGTCGTCCT 
                     CTGGCGCTGGTGCGTACTCACAGTAAGAAAGCACTGATGCGCTACGAAGACGTTTACA 
                     TGCCTGAGGTGTACAAAGCGATTAACATTGCGCAAAACACCGCATGGAAAATCAACAA 
                     GAAAGTCCTAGCGGTCGCCAACGTAATCACCAAGTGGAAGCATTGTCCGGTCGAGGAC 
                     ATCCCTGCGATTGAGCGTGAAGAACTCCCGATGAAACCGGAAGACATCGACATGAATC 
                     CTGAGGCTCTCACCGCGTGGAAACGTGCTGCCGCTGCTGTGTACCGCAAGGACAAGGC 
                     TCGCAAGTCTCGCCGTATCAGACTTGAGTTCATGCTTGAGCAAGCCAATAAGTTTGCT 
                     AACCATAAGGCCATCTGGTTCCCTTACAACATGGACTGGCGCGGTCGTGTTTACGCTG 
                     TGTCAATGTTCAACCCGCAAGGTAACGATATGACCAAAGGACTGCTTACGCTGGCGAA 
                     AGGTAAACCAATCGGTAAGGAAGGTTACTACTGGCTGAAAATCCACGGTGCAAACTGT 
                     GCGGGTGTCGATAAGGTTCCGTTCCCTGAGCGCATCAAGTTCATTGAGGAAAACCACG 
                     AGAACATCATGGCTTGCGCTAAGTCTCCACTGGAGAACACTTGGTGGGCTGAGCAAGA 
                     TTCTCCGTTCTGCTTCCTTGCGTTCTGCTTTGAGTACGCTGGGGTACAGCACCACGGC 
                     CTGAGCTATAACTGCTCCCTTCCGCTGGCGTTTGACGGGTCTTGCTCTGTCATCCAGC 
                     ACTTCTCCGCGATGCTCCGAGATGAGGTAGGTGGTCGCGCGGTTAACTTGCTTCCTAG 
                     TGAAACCGTTCAGGACATCTACGGGATTGTTGCTAAGAAAGTCAACGAGATTCTACAA 
                     GCAGACGCAATCAATGGGACCGATAACGAAGTAGTTACCGTGACCGATGAGAACACTG 
                     GTGAAATCTCTGAGAAAGTCAAGCTGGGCACTAAGGCACTGGCTGGTCAATGGCTGGC 
                     TTACGGTGTTACTCGCAGTGTGACTAAGCGTTCAGTCATGACGCTGGCTTACGGGTCC 
                     AAAGAGTTCGGCTTCCGTCAACAAGTGCTGGACGATACCATTCAGCCAGCTATTGATT 
                     CCGGCAAGGGTCTGATGTTCACCCAGCCGAATCAGGCTGCTAGATACATGGCTAAGCT 
                     GATTTGGGAATCTGTGAGCGTGACGGTGGTAGCTGCGGTTGAAGCAATGAACTGGCTT 
                     AAGTCTGCTGCTAAGCTGCTGGCTGCTGAGGTCAAAGATAAGAAGACTGGAGAGATTC 
                     TTCGCAAGCGTTGCGCTGTGCATTGGATAACTCCTGATGGTTTCCCTGTGTGGCAGGA 
                     ATACAAGAAGCCTATTCAGACGCGCTTGGACCTGATGTTCCTCGGTCAGTTCCGCTTA 
                     CAGCTTACCATTAACACCAACAAAGATAGCGAGATTGATGCACACAAACAGGAGG"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5536..5715
                     /label=vamp1_60aa
                     /note="vamp1_60aa"
                     /note="Geneious type: CDS"
     CDS             5716..8364
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
     misc_feature    5716..5740
                     /label=MSP913
                     /note="MSP913"
     misc_feature    5748..5767
                     /standard_name="TRB137"
                     /label=TRB137
                     /note="Geneious type: PCR_primer"
     misc_feature    5970..5996
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    5970..5996
                     /label=1253
                     /note="1253"
     misc_feature    6023..6042
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    6023..6042
                     /label=1259
                     /note="1259"
     misc_feature    6152..6185
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    6252..6271
                     /standard_name="TRB138"
                     /label=TRB138
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(6342..6364)
                     /standard_name="bcd1175"
                     /label=bcd1175
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    complement(6346..6367)
                     /standard_name="TRB211"
                     /label=TRB211
                     /note="Geneious type: PCR_primer"
     misc_feature    6349..6366
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    6419..6448
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    6503..6531
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(6666..6697)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    6767..6788
                     /standard_name="BCD1260 (TRB139)"
                     /label=BCD1260 (TRB139)
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    6767..6788
                     /label=1260
                     /note="1260"
     misc_feature    7018..7047
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    7029..7063
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    7098..7116
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    7282..7301
                     /standard_name="TRB140"
                     /label=TRB140
                     /note="Geneious type: PCR_primer"
     misc_feature    7401..7428
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    7474..7496
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    7474..7496
                     /label=1261
                     /note="1261"
     misc_feature    complement(7474..7496)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    complement(7599..7626)
                     /label=MSP859
                     /note="MSP859"
     misc_feature    complement(7615..7663)
     misc_feature    7615..7641
                     /label=MSP860
                     /note="MSP860"
     misc_feature    7618..7647
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    7681..7701
                     /label=MSP63
                     /note="MSP63"
     misc_feature    7702..7722
                     /label=MSP62
                     /note="MSP62"
     misc_feature    7788..7807
                     /standard_name="TRB141"
                     /label=TRB141
                     /note="Geneious type: PCR_primer"
     misc_feature    7820..7838
                     /standard_name="TRB141a"
                     /label=TRB141a
                     /note="Geneious type: PCR_primer"
     misc_feature    8044..8066
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    8044..8066
                     /label=1262
                     /note="1262"
     misc_feature    8282..8302
                     /standard_name="TRB142"
                     /label=TRB142
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(8343..8380)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    8370..8394
                     /standard_name="TRB300"
                     /label=TRB300
                     /note="Geneious type: PCR_primer"
     CDS             8379..8561
                     /standard_name="III fragment"
                     /label=III fragment
     promoter        8434..8461
                     /standard_name="internal promoter"
                     /label=internal promoter
                     /note="Geneious type: Promoter"
     misc_feature    8480..8510
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD616"
     misc_feature    complement(8480..8510)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD617"
     misc_feature    8480..8510
                     /label=BCD616
                     /note="BCD616"
     misc_feature    8480..8510
                     /label=BCD617
                     /note="BCD617"
     misc_feature    complement(8532..8561)
                     /label=MSP64
                     /note="MSP64"
     CDS             8564..8590
                     /standard_name="VI fragment"
                     /label=VI fragment
     misc_feature    8578..8597
                     /label=1311
                     /note="1311"
     misc_feature    8598..8618
                     /label=BCD1310
                     /note="BCD1310"
     CDS             8604..9392
                     /standard_name="aadA"
                     /label=aadA
     misc_feature    8821..8850
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1251"
     misc_feature    8821..8850
                     /label=1251
                     /note="1251"
     misc_feature    8943..8965
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1255"
     misc_feature    8943..8965
                     /label=1255
                     /note="1255"
     misc_feature    complement(9020..9049)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1254"
     misc_feature    complement(9020..9049)
                     /label=1254
                     /note="1254"
ORIGIN
        1 acggatcgct tcatgtggca ggagaaaaaa gactgcaccg gtgcgtcagc agaatatgtg
       61 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
      121 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
      181 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
      241 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      361 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
      421 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
      481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
      541 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
      601 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
      661 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
      721 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg
      781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1081 cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc ggggatcgac
     1141 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     1201 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc
     1261 aagtcagcgt aatgctctgc cagtgttaca accaattaac aagaggacat ccggtcaaat
     1321 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt gctgaggaga cttagggacc
     1381 ctactaatac gactcactat agggagaaag aaggagcgac attgctccgt gtattcactc
     1441 gttggaatga atacacagtg cagtgtttat tctgttattt atgccaaaaa taaaggccac
     1501 tatcaggcag ctttgttgtt ctgtttacca agttcaggag gtaactcata agaaagacct
     1561 gcaggtgcag taaaaaaaaa ggaaaatgaa aaaattatta ttcgcaattc ctttagttgt
     1621 tcctttctat tctcactccg ctgaaactgt tcatcaccat caccatcacg ctgaaactgt
     1681 tgaaagttgt ttagcaaaac cccatacaga aaattcattt actaacgtct ggaaagacga
     1741 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgcta caggcgttgt
     1801 agtttgtact ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat
     1861 ccctgaaaat gagggtggtg gctctgaggg tggcggttct gagggtggcg gttctgaggg
     1921 tggcggtact aaacctcctg agtacggtga tacacctatt ccgggctata cttatatcaa
     1981 ccctctcgac ggcacttatc cgcctggtac tgagcaaaac cccgctaatc ctaatccttc
     2041 tcttgaggag tctcagcctc ttaatacttt catgtttcag aataataggt tccgaaatag
     2101 gcagggggca ttaactgttt atacgggcac tgttactcaa ggcactgacc ccgttaaaac
     2161 ttattaccag tacactcctg tatcatcaaa agccatgtat gacgcttact ggaacggtaa
     2221 attcagagac tgcgctttcc attctggctt taatgaggat ccattcgttt gtgaatatca
     2281 aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct ctggtggtgg
     2341 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     2401 tgagggaggc ggttccggtg gtggctcttc ccaaatggct caagtcggtg acggtgataa
     2461 ttcaccttta atgaataatt tccgtcaata tttaccttcc ctccctcaat cggttgaatg
     2521 tcgccctttt gtctttggcg ctggtaaacc ttacgagttc agtatcgact gcgataagat
     2581 caacctgttc cgcggtgtct ttgcgtttct tttatatgtt gccaccttta tgtatgtatt
     2641 ttctacgttt gctaacatac tgcgtaataa ggagtcttaa tgaaatttgg aaactttttg
     2701 cttacatacc aacctcccca attttcccaa acagaggtaa tgaaacgttt ggttaaatta
     2761 ggtcgcatct ctgaggagtg tggttttgat accgtatggt tactggagca tcatttcacg
     2821 gagtttggtt tgcttggtaa cccttatgtc gctgctgcat atttacttgg cgcgactaaa
     2881 aaattgaatg taggaactgc cgctattgtt cttcccacag cccatccagt acgccaactt
     2941 gaagatgtga atttattgga tcaaatgtca aaaggacgat ttcggtttgg tatttgccga
     3001 gggctttaca acaaggactt tcgcgtattc ggcacagata tgaataacag tcgcgcctta
     3061 gcggaatgct ggtacgggct gataaagaat ggcatgacag agggatatat ggaagctgat
     3121 aatgaacata tcaagttcca taaggtaaaa gtaaaccccg cggcgtatag cagaggtggc
     3181 gcaccggttt atgtggtggc tgaatcagct tcgacgactg agtgggctgc tcaatttggc
     3241 ctaccgatga tattaagttg gattataaat actaacgaaa agaaagcaca acttgagctt
     3301 tataatgaag tggctcaaga atatgggcac gatattcata atatcgacca ttgcttatca
     3361 tatataacat ctgtagatca tgactcaatt aaagcgaaag agatttgccg gaaatttctg
     3421 gggcattggt atgattctta tgtgaatgct acgactattt ttgatgattc agaccaaaca
     3481 agaggttatg atttcaataa agggcagtgg cgtgactttg tattaaaagg acataaagat
     3541 actaatcgcc gtattgatta cagttacgaa atcaatcccg tgggaacgcc gcaggaatgt
     3601 attgacataa ttcaaaaaga cattgatgct acaggaatat caaatatttg ttgtggattt
     3661 gaagctaatg gaacagtaga cgaaattatt gcttccatga agctcttcca gtctgatgtc
     3721 atgccatttc ttaaagaaaa acaacgttcg ctattatatt atggcggtgg cggtagcggc
     3781 ggtggcggta gcggcggtgg cggtagcggc ggtggcggta gcaaatttgg attgttcttc
     3841 cttaacttca tcaattcaac aactgttcaa gaacagagta tagttcgcat gcaggaaata
     3901 acggagtatg ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca
     3961 gataatggtg ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag
     4021 aaaattaaaa ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg
     4081 gaggaagctt gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat
     4141 tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta
     4201 tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat
     4261 aacgattttt atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga
     4321 cctcggaaat atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt
     4381 attcctctca tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga
     4441 tataaagccg ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg
     4501 atattagtta actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt
     4561 agtgattatg ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata
     4621 attgcagaaa acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt
     4681 gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc
     4741 caaaaaaatg taatcaatat tgttgatgat aatattaaga agtaccacac ggaatatacc
     4801 taaacttaat taacggcact cctcagcaaa tataatgacc ctcttgataa cccaagaggg
     4861 cattttttaa tgcccatggc gtcaattccg atttccggta ttctagagca cagctaacac
     4921 cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca
     4981 tgcataaggc tcgtataata tattcaggga gaccacaacg gtttccctct acaaataatt
     5041 ttgtttaact tttactagag ttccttcctc gaaaggaaaa aaaaaatggc tcgtgtacag
     5101 tttaaacaac gtgaatctac tgacgcaatc tttgttcact gctcggctac caagccaagt
     5161 cagaatgttg gtgtccgtga gattcgccag tggcacaaag agcagggttg gctcgatgtg
     5221 ggataccact ttatcatcaa gcgagacggt actgtggagg caggacgaga tgagatggct
     5281 gtaggctctc acgctaaggg ttacaaccac aactctatcg gcgtctgcct tgttggtggt
     5341 atcgacgata aaggtaagtt cgacgctaac tttacgccag cccaaatgca atcccttcgc
     5401 tcactgcttg tcacactgct ggctaagtac gaaggcgctg tgcttcgcgc ccatcatgag
     5461 gtggcgccga aggcttcccc ttcgttcgac cttaagcgtt ggtgggagaa gaacgaactg
     5521 gtcacatctg accgtacctc taaccgtcgt ctgcagcaga cccaggctca ggttgaagaa
     5581 gttgttgaca tcatccgtgt taacgttgac aaagttctgg aacgtgacca gaaactgtct
     5641 gaactggacg accgtgctga cgctctgcag gctggtgctt ctcagttcga atcttctgct
     5701 gctaaactga aacgtaacac gattaacatc gctaagaacg acttctctga catcgaactg
     5761 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag
     5821 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag
     5881 cgtcaactta aagctggtga ggttgcggat aacgctgccg ctaagcctct catcactacc
     5941 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc
     6001 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac
     6061 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct
     6121 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac
     6181 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac
     6241 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc
     6301 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc
     6361 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta
     6421 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc
     6481 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag
     6541 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg
     6601 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg
     6661 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg
     6721 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag
     6781 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg
     6841 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc
     6901 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc
     6961 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt
     7021 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt
     7081 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct
     7141 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca
     7201 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt
     7261 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt
     7321 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt
     7381 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag
     7441 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc
     7501 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg
     7561 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg
     7621 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag
     7681 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac
     7741 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg
     7801 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga
     7861 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg
     7921 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc
     7981 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct
     8041 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg
     8101 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc
     8161 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat
     8221 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct
     8281 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta
     8341 gagtcggact tcgcgttcgc gtaatggaga ttttcaacat gctccctcaa tcggttgaat
     8401 gtcgcccttt tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa
     8461 taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat
     8521 tttctacgtt tgctaacata ctgcgtaata aggagtctta atcatgccag ttcttttggg
     8581 tattccgtag aaaaaggaag agtatgaggg aagcggtgat cgccgaagta tcgactcaac
     8641 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     8701 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     8761 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     8821 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     8881 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     8941 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     9001 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     9061 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     9121 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     9181 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     9241 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     9301 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     9361 tgaaaggcga gatcaccaag gtagtcggca aa
//
