LOCUS       Exported                9334 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  T7neg-122-1137-proD.
ACCESSION   urn.local...3qp-d2b7044
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4915..5059
     misc_feature    1..861
                     /standard_name="p15A origin"
                     /label=p15A origin
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(260..282)
                     /standard_name="TRB839"
                     /label=TRB839
                     /note="Geneious type: PCR_primer"
     misc_feature    320..342
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1256"
     misc_feature    320..342
                     /label=1256
                     /note="1256"
     misc_feature    389..409
                     /standard_name="TRB840"
                     /label=TRB840
                     /note="Geneious type: PCR_primer"
     misc_feature    944..970
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1257"
     misc_feature    944..970
                     /label=1257
                     /note="1257"
     misc_feature    1280..1315
     misc_feature    complement(1280..1309)
                     /label=MSP369
                     /note="MSP369"
     misc_feature    1287..1315
                     /label=MSP370
                     /note="MSP370"
     modified_base   1301..1315
                     /label=USER junction
                     /note="USER junction"
     terminator      1316..1360
                     /note="rrnB1 transcriptional terminator"
     misc_feature    1361..1367
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     misc_feature    complement(1362..1400)
                     /standard_name="TRB302"
                     /label=TRB302
                     /note="Geneious type: PCR_primer"
     modified_base   1368..1382
                     /label=USER junction
                     /note="USER junction"
     misc_feature    1375..1381
                     /label=SanDI
                     /note="SanDI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange f1 origin or selection components"
     misc_feature    1381..1404
     misc_feature    1383..1425
                     /standard_name="TRB303"
                     /label=TRB303
                     /note="Geneious type: PCR_primer"
     promoter        1385..1404
                     /note="T7 promoter"
     misc_feature    1385..1400
                     /standard_name="AATTAACCCTCACTAA"
                     /label=AATTAACCCTCACTAA
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1390..1413
     terminator      1412..1557
                     /standard_name="T1 terminator"
                     /label=T1 terminator
     misc_feature    complement(1541..1557)
     misc_feature    1558..1565
                     /label=SbfI
                     /note="SbfI"
     misc_feature    1565..1570
                     /label=BsgI
                     /note="BsgI"
     RBS             1572..1584
                     /standard_name="sd7"
                     /label=sd7
                     /note="RBS sd8"
     CDS             1585..2679
                     /standard_name="P3NEG"
                     /label=P3NEG
     misc_feature    1617
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1620
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     exon            1639..1869
                     /note="N1 Domain"
     exon            1927..2319
                     /note="N2 Domain"
     misc_feature    1985..2014
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD705"
     exon            2431..2676
                     /note="CT Domain (342 - 424)"
     misc_feature    2535..2568
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd706"
     misc_feature    2547..2592
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     misc_feature    complement(2650..2679)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP64"
     misc_feature    2650..2679
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP356"
     CDS             2679..4802
                     /label=xluxAB
                     /note="xluxAB"
     sig_peptide     3762..3821
                     /note="GGGGS4 flexible linker"
     misc_feature    complement(4113..4143)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP14"
     misc_feature    4113..4143
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="MSP15"
     misc_feature    4136..4164
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="708"
     modified_base   4803..4819
                     /label=USER junction
                     /note="USER junction"
     misc_feature    complement(4803..4819)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
     misc_feature    4805..4812
                     /label=PacI
                     /note="PacI"
                     /note="Keep unique in all constructs, can be used 
                     toexchange antibiotic resistance marker or selection 
                     components"
     misc_feature    4820..4826
                     /label=Nt-BbvCI
                     /note="Nt-BbvCI"
                     /note="nicking endonuclease site that generates overhang"
     terminator      complement(4827..4868)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weakerthan
                     opposite direction"
     misc_feature    4867..4898
     misc_feature    complement(4867..4896)
                     /label=MSP368
                     /note="MSP368"
     modified_base   4869..4881
                     /label=USER junction
                     /note="USER junction"
     misc_feature    4870..4898
                     /label=MSP367
                     /note="MSP367"
     misc_feature    complement(4877..4908)
                     /label=MSP420
                     /note="MSP420"
     misc_feature    4888
                     /standard_name="T"
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4889..4923
                     /label=MSP419
                     /note="MSP419"
     misc_feature    4899..5059
     primer_bind     4899..4929
                     /standard_name="DB294"
                     /label=DB294
     primer_bind     complement(4899..4914)
                     /standard_name="AB371 primer bind"
                     /label=AB371 primer bind
     primer_bind     complement(4899..4914)
                     /standard_name="DB195"
                     /label=DB195
     misc_feature    4899..4914
                     /standard_name="USER junction"
                     /label=USER junction
     primer_bind     4941..4992
                     /label=AB365
                     /note="AB365"
     promoter        4964..5004
                     /standard_name="ProD"
                     /label=ProD promoter
                     /note="proD (Insulated Promoter - based on rrnB1 P1)"
     misc_feature    complement(4969..4992)
     -35_signal      4970..4975
                     /label=-35
                     /note="-35"
     misc_feature    4974..5018
     primer_bind     complement(4976..5027)
                     /label=AB366
                     /note="AB366"
     -10_signal      4993..4998
                     /label=-10
                     /note="-10"
     prim_transcript 5005
                     /label=+1
                     /note="+1"
     primer_bind     complement(5012..5059)
                     /standard_name="AB367"
                     /label=AB367
     misc_feature    complement(5024..5052)
                     /standard_name="TRB304"
                     /label=TRB304
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(5026..5067)
                     /label=MSP382
                     /note="MSP382"
     misc_feature    5037..5063
                     /standard_name="TRB305"
                     /label=TRB305
                     /note="Geneious type: PCR_primer"
     modified_base   5037..5059
     primer_bind     5037..5059
                     /standard_name="DB293"
                     /label=DB293
     misc_feature    5048..5084
                     /label=MSP381
                     /note="MSP381"
     RBS             5071..5084
                     /standard_name="sd8"
                     /label=sd8
     CDS             5085..5534
                     /standard_name="lysozyme"
                     /label=lysozyme
     misc_feature    5192..5217
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1258"
     misc_feature    5192..5217
                     /label=BCD1258
                     /note="BCD1258"
     misc_feature    complement(5514..5534)
                     /label=MSP912
                     /note="MSP912"
     misc_feature    5525..7980
                     /standard_name="TTCTGACCGTGGTAGCGGCGGTGGTGCGAGTGGTGGCGCGCT 
                     GGAGGTCCTGTTCCAGGGCCCGGGCGGTAGCGCAGGCAGTGGAGCGGGCGGTAACACG 
                     ATTAACATCGCTAAGAACGACTTCTCTGACATCGAACTGGCTGCTATCCCGTTCAACA 
                     CTCTGGCTGACCATTACGGTGAGCGTTTAGCTCGCGAACAGTTGGCCCTTGAGCATGA 
                     GTCTTACGAGATGGGTGAAGCACGCTTCCGCAAGATGTTTGAGCGTCAACTTAAAGCT 
                     GGTGAGGTTGCGGATAACGCTGCCGCCAAGCCTCTCATCACTACCCTACTCCCTAAGA 
                     TGATTGCACGCATCAACGACTGGTTTGAGGAAGTGAAAGCTAAGCTCGGCAGGCGCCC 
                     GACAGCCTTCCAGTTCCTGCAAGAAATCAAGCCGGAAGCCGTAGCGTACATCACCATT 
                     AAGACCACTCTGGCTTGCCTAACCAGTGCTGACAATACAACCGTTCAGGCTGTAGCAA 
                     GCGCAATCGGTCGGGCCATTGAGGACGAGGCTCGCTTCGGTCGTATCCGTGACCTTGA 
                     AGCTAAGCACTTCAAGAAAAACGTTGAGGAACAACTCAACAAGCGCGTAGGGCACGTC 
                     TACAAGAAAGCATTTATGCAAGTTGTCGAGGCTGACATGCTCTCTAAGGGTCTACTCG 
                     GTGGCGAGGCGTGGTCTTCGTGGTATAAGAAAGACTCTATTCATGTAGGAGTACGCTG 
                     CATCGAGATGCTCATTAAGTCAACTGGAATGGTTAGCTTACACCGCCAAAATGCTGGC 
                     GTAGTAGGTCAAGACTCTGAGACTATCGAACTCGCACCTGAATACGCTGAGGCTATCG 
                     CAACCCGTGCAGGTGCGCTGGCTGGCATCTCTCCGATGTTCCAACCTTGCGTAGTTCC 
                     TCCTAAGCCGTGGACTGGCATTACTGGTGGTGGCTATTGGGCTAACGGTCGTCGTCCT 
                     CTGGCGCTGGTGCGTACTCACAGTAAGAAAGCACTGATGCGCTACGAAGACGTTTACA 
                     TGCCTGAGGTGTACAAAGCGATTAACATTGCGCAAAACACCGCATGGAAAATCAACAA 
                     GAAAGTCCTAGCGGTCGCCAACGTAATCACCAAGTGGAAGCATTGTCCGGTCGAGGAC 
                     ATCCCTGCGATTGAGCGTGAAGAACTCCCGATGAAACCGGAAGACATCGACATGAATC 
                     CTGAGGCTCTCACCGCGTGGAAACGTGCTGCCGCTGCTGTGTACCGCAAGGACAAGGC 
                     TCGCAAGTCTCGCCGTATCAGACTTGAGTTCATGCTTGAGCAAGCCAATAAGTTTGCT 
                     AACCATAAGGCCATCTGGTTCCCTTACAACATGGACTGGCGCGGTCGTGTTTACGCTG 
                     TGTCAATGTTCAACCCGCAAGGTAACGATATGACCAAAGGACTGCTTACGCTGGCGAA 
                     AGGTAAACCAATCGGTAAGGAAGGTTACTACTGGCTGAAAATCCACGGTGCAAACTGT 
                     GCGGGTGTCGATAAGGTTCCGTTCCCTGAGCGCATCAAGTTCATTGAGGAAAACCACG 
                     AGAACATCATGGCTTGCGCTAAGTCTCCACTGGAGAACACTTGGTGGGCTGAGCAAGA 
                     TTCTCCGTTCTGCTTCCTTGCGTTCTGCTTTGAGTACGCTGGGGTACAGCACCACGGC 
                     CTGAGCTATAACTGCTCCCTTCCGCTGGCGTTTGACGGGTCTTGCTCTGTCATCCAGC 
                     ACTTCTCCGCGATGCTCCGAGATGAGGTAGGTGGTCGCGCGGTTAACTTGCTTCCTAG 
                     TGAAACCGTTCAGGACATCTACGGGATTGTTGCTAAGAAAGTCAACGAGATTCTACAA 
                     GCAGACGCAATCAATGGGACCGATAACGAAGTAGTTACCGTGACCGATGAGAACACTG 
                     GTGAAATCTCTGAGAAAGTCAAGCTGGGCACTAAGGCACTGGCTGGTCAATGGCTGGC 
                     TTACGGTGTTACTCGCAGTGTGACTAAGCGTTCAGTCATGACGCTGGCTTACGGGTCC 
                     AAAGAGTTCGGCTTCCGTCAACAAGTGCTGGACGATACCATTCAGCCAGCTATTGATT 
                     CCGGCAAGGGTCTGATGTTCACCCAGCCGAATCAGGCTGCTAGATACATGGCTAAGCT 
                     GATTTGGGAATCTGTGAGCGTGACGGTGGTAGCTGCGGTTGAAGCAATGAACTGGCTT 
                     AAGTCTGCTGCTAAGCTGCTGGCTGCTGAGGTCAAAGATAAGAAGACTGGAGAGATTC 
                     TTCGCAAGCGTTGCGCTGTGCATTGGATAACTCCTGATGGTTTCCCTGTGTGGCAGGA 
                     ATACAAGAAGCCTATTCAGACGCGCTTGGACCTGATGTTCCTCGGTCAGTTCCGCTTA 
                     CAGCTTACCATTAACACCAACAAAGATAGCGAGATTGATGCACACAAACAGGAGG"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     CDS             5535..5564
                     /standard_name="Linker"
                     /label=Linker
     misc_feature    complement(5535..5544)
                     /label=MSP440
                     /note="MSP440"
     CDS             5565..5627
                     /standard_name="SNAP25"
                     /label=SNAP25
     CDS             5628..5657
                     /standard_name="Linker"
                     /label=Linker
     CDS             5658..8306
                     /standard_name="T7RNAP"
                     /label=T7RNAP
     misc_feature    5658..5682
                     /label=MSP913
                     /note="MSP913"
     misc_feature    5690..5709
                     /standard_name="TRB137"
                     /label=TRB137
                     /note="Geneious type: PCR_primer"
     misc_feature    5912..5938
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1253"
     misc_feature    5912..5938
                     /label=1253
                     /note="1253"
     misc_feature    5965..5984
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1259"
     misc_feature    5965..5984
                     /label=1259
                     /note="1259"
     misc_feature    6094..6127
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD710"
     misc_feature    6194..6213
                     /standard_name="TRB138"
                     /label=TRB138
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(6284..6306)
                     /standard_name="bcd1175"
                     /label=bcd1175
                     /note="Geneious type: PCR_primer"
                     /note="bcd1175"
     misc_feature    complement(6288..6309)
                     /standard_name="TRB211"
                     /label=TRB211
                     /note="Geneious type: PCR_primer"
     misc_feature    6291..6308
                     /standard_name="SeqT7-1"
                     /label=SeqT7-1
                     /note="Geneious type: PCR_primer"
     misc_feature    6361..6390
                     /label=t7seq2b
                     /note="t7seq2b"
     misc_feature    6445..6473
                     /label=T7seq2
                     /note="T7seq2"
     misc_feature    complement(6608..6639)
                     /label=T7seq1
                     /note="T7seq1"
     misc_feature    6709..6730
                     /standard_name="BCD1260 (TRB139)"
                     /label=BCD1260 (TRB139)
                     /note="Geneious type: PCR_primer"
                     /note="BCD1260"
     misc_feature    6709..6730
                     /label=1260
                     /note="1260"
     misc_feature    6960..6989
                     /label=t7seq2c
                     /note="t7seq2c"
     misc_feature    6971..7005
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd711"
     misc_feature    7040..7058
                     /standard_name="SeqT7-2"
                     /label=SeqT7-2
                     /note="Geneious type: PCR_primer"
     misc_feature    7224..7243
                     /standard_name="TRB140"
                     /label=TRB140
                     /note="Geneious type: PCR_primer"
     misc_feature    7343..7370
                     /label=T7 seq3
                     /note="T7 seq3"
     misc_feature    7416..7438
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCd1261"
     misc_feature    7416..7438
                     /label=1261
                     /note="1261"
     misc_feature    complement(7416..7438)
                     /label=MSP347
                     /note="MSP347"
     misc_feature    complement(7541..7568)
                     /label=MSP859
                     /note="MSP859"
     misc_feature    complement(7557..7605)
     misc_feature    7557..7583
                     /label=MSP860
                     /note="MSP860"
     misc_feature    7560..7589
                     /label=T7seq3b
                     /note="T7seq3b"
     misc_feature    7623..7643
                     /label=MSP63
                     /note="MSP63"
     misc_feature    7644..7664
                     /label=MSP62
                     /note="MSP62"
     misc_feature    7730..7749
                     /standard_name="TRB141"
                     /label=TRB141
                     /note="Geneious type: PCR_primer"
     misc_feature    7762..7780
                     /standard_name="TRB141a"
                     /label=TRB141a
                     /note="Geneious type: PCR_primer"
     misc_feature    7986..8008
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1262"
     misc_feature    7986..8008
                     /label=1262
                     /note="1262"
     misc_feature    8224..8244
                     /standard_name="TRB142"
                     /label=TRB142
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(8285..8322)
                     /standard_name="TRB301"
                     /label=TRB301
                     /note="Geneious type: PCR_primer"
     misc_feature    8312..8336
                     /standard_name="TRB300"
                     /label=TRB300
                     /note="Geneious type: PCR_primer"
     CDS             8321..8503
                     /standard_name="III fragment"
                     /label=III fragment
     promoter        8376..8403
                     /standard_name="internal promoter"
                     /label=internal promoter
                     /note="Geneious type: Promoter"
     misc_feature    8422..8452
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD616"
     misc_feature    complement(8422..8452)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD617"
     misc_feature    8422..8452
                     /label=BCD616
                     /note="BCD616"
     misc_feature    8422..8452
                     /label=BCD617
                     /note="BCD617"
     misc_feature    complement(8474..8503)
                     /label=MSP64
                     /note="MSP64"
     CDS             8506..8532
                     /standard_name="VI fragment"
                     /label=VI fragment
     misc_feature    8520..8539
                     /label=1311
                     /note="1311"
     misc_feature    8540..8560
                     /label=BCD1310
                     /note="BCD1310"
     CDS             8546..9334
                     /standard_name="aadA"
                     /label=aadA
     misc_feature    8763..8792
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1251"
     misc_feature    8763..8792
                     /label=1251
                     /note="1251"
     misc_feature    8885..8907
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="bcd1255"
     misc_feature    8885..8907
                     /label=1255
                     /note="1255"
     misc_feature    complement(8962..8991)
                     /label=Geneious type: PCR_primer
                     /note="Geneious type: PCR_primer"
                     /note="BCD1254"
     misc_feature    complement(8962..8991)
                     /label=1254
                     /note="1254"
ORIGIN
        1 acggatcgct tcatgtggca ggagaaaaaa gactgcaccg gtgcgtcagc agaatatgtg
       61 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
      121 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
      181 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
      241 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      361 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
      421 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
      481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
      541 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
      601 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
      661 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
      721 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg
      781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1081 cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc ggggatcgac
     1141 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     1201 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc
     1261 aagtcagcgt aatgctctgc cagtgttaca accaattaac aagaggacat ccggtcaaat
     1321 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt gctgaggaga cttagggacc
     1381 ctactaatac gactcactat agggagaaag aaggagcgac attgctccgt gtattcactc
     1441 gttggaatga atacacagtg cagtgtttat tctgttattt atgccaaaaa taaaggccac
     1501 tatcaggcag ctttgttgtt ctgtttacca agttcaggag gtaactcata agaaagacct
     1561 gcaggtgcag taaaggaaaa aaaaatgaaa aaattattat tcgcaattcc tttagttgtt
     1621 cctttctatt ctcactccgc tgaaactgtt catcaccatc accatcacgc tgaaactgtt
     1681 gaaagttgtt tagcaaaacc ccatacagaa aattcattta ctaacgtctg gaaagacgac
     1741 aaaactttag atcgttacgc taactatgag ggctgtctgt ggaatgctac aggcgttgta
     1801 gtttgtactg gtgacgaaac tcagtgttac ggtacatggg ttcctattgg gcttgctatc
     1861 cctgaaaatg agggtggtgg ctctgagggt ggcggttctg agggtggcgg ttctgagggt
     1921 ggcggtacta aacctcctga gtacggtgat acacctattc cgggctatac ttatatcaac
     1981 cctctcgacg gcacttatcc gcctggtact gagcaaaacc ccgctaatcc taatccttct
     2041 cttgaggagt ctcagcctct taatactttc atgtttcaga ataataggtt ccgaaatagg
     2101 cagggggcat taactgttta tacgggcact gttactcaag gcactgaccc cgttaaaact
     2161 tattaccagt acactcctgt atcatcaaaa gccatgtatg acgcttactg gaacggtaaa
     2221 ttcagagact gcgctttcca ttctggcttt aatgaggatc cattcgtttg tgaatatcaa
     2281 ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg gcggcggctc tggtggtggt
     2341 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct
     2401 gagggaggcg gttccggtgg tggctcttcc caaatggctc aagtcggtga cggtgataat
     2461 tcacctttaa tgaataattt ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt
     2521 cgcccttttg tctttggcgc tggtaaacct tacgagttca gtatcgactg cgataagatc
     2581 aacctgttcc gcggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt
     2641 tctacgtttg ctaacatact gcgtaataag gagtcttaat gaaatttgga aactttttgc
     2701 ttacatacca acctccccaa ttttcccaaa cagaggtaat gaaacgtttg gttaaattag
     2761 gtcgcatctc tgaggagtgt ggttttgata ccgtatggtt actggagcat catttcacgg
     2821 agtttggttt gcttggtaac ccttatgtcg ctgctgcata tttacttggc gcgactaaaa
     2881 aattgaatgt aggaactgcc gctattgttc ttcccacagc ccatccagta cgccaacttg
     2941 aagatgtgaa tttattggat caaatgtcaa aaggacgatt tcggtttggt atttgccgag
     3001 ggctttacaa caaggacttt cgcgtattcg gcacagatat gaataacagt cgcgccttag
     3061 cggaatgctg gtacgggctg ataaagaatg gcatgacaga gggatatatg gaagctgata
     3121 atgaacatat caagttccat aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg
     3181 caccggttta tgtggtggct gaatcagctt cgacgactga gtgggctgct caatttggcc
     3241 taccgatgat attaagttgg attataaata ctaacgaaaa gaaagcacaa cttgagcttt
     3301 ataatgaagt ggctcaagaa tatgggcacg atattcataa tatcgaccat tgcttatcat
     3361 atataacatc tgtagatcat gactcaatta aagcgaaaga gatttgccgg aaatttctgg
     3421 ggcattggta tgattcttat gtgaatgcta cgactatttt tgatgattca gaccaaacaa
     3481 gaggttatga tttcaataaa gggcagtggc gtgactttgt attaaaagga cataaagata
     3541 ctaatcgccg tattgattac agttacgaaa tcaatcccgt gggaacgccg caggaatgta
     3601 ttgacataat tcaaaaagac attgatgcta caggaatatc aaatatttgt tgtggatttg
     3661 aagctaatgg aacagtagac gaaattattg cttccatgaa gctcttccag tctgatgtca
     3721 tgccatttct taaagaaaaa caacgttcgc tattatatta tggcggtggc ggtagcggcg
     3781 gtggcggtag cggcggtggc ggtagcggcg gtggcggtag caaatttgga ttgttcttcc
     3841 ttaacttcat caattcaaca actgttcaag aacagagtat agttcgcatg caggaaataa
     3901 cggagtatgt tgataagttg aattttgaac agattttagt gtatgaaaat catttttcag
     3961 ataatggtgt tgtcggcgct cctctgactg tttctggttt tctgctcggt ttaacagaga
     4021 aaattaaaat tggttcatta aatcacatca ttacaactca tcatcctgtc cgcatagcgg
     4081 aggaagcttg cttattggat cagttaagtg aagggagatt tattttaggg tttagtgatt
     4141 gcgaaaaaaa agatgaaatg cattttttta atcgcccggt tgaatatcaa cagcaactat
     4201 ttgaagagtg ttatgaaatc attaacgatg ctttaacaac aggctattgt aatccagata
     4261 acgattttta tagcttccct aaaatatctg taaatcccca tgcttatacg ccaggcggac
     4321 ctcggaaata tgtaacagca accagtcatc atattgttga gtgggcggcc aaaaaaggta
     4381 ttcctctcat ctttaagtgg gatgattcta atgatgttag atatgaatat gctgaaagat
     4441 ataaagccgt tgcggataaa tatgacgttg acctatcaga gatagaccat cagttaatga
     4501 tattagttaa ctataacgaa gatagtaata aagctaaaca agagacgcgt gcatttatta
     4561 gtgattatgt tcttgaaatg caccctaatg aaaatttcga aaataaactt gaagaaataa
     4621 ttgcagaaaa cgctgtcgga aattatacgg agtgtataac tgcggctaag ttggcaattg
     4681 aaaagtgtgg tgcgaaaagt gtattgctgt cctttgaacc aatgaatgat ttgatgagcc
     4741 aaaaaaatgt aatcaatatt gttgatgata atattaagaa gtaccacacg gaatatacct
     4801 aaacttaatt aacggcactc ctcagcaaat ataatgaccc tcttgataac ccaagagggc
     4861 attttttaat gcccatggcg tcaattccga tttccggtat tctagagcac agctaacacc
     4921 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
     4981 gcataaggct cgtataatat attcagggag accacaacgg tttccctcta caaataattt
     5041 tgtttaactt ttactagagt tccttcctcg aaaggaaaaa aaaaatggct cgtgtacagt
     5101 ttaaacaacg tgaatctact gacgcaatct ttgttcactg ctcggctacc aagccaagtc
     5161 agaatgttgg tgtccgtgag attcgccagt ggcacaaaga gcagggttgg ctcgatgtgg
     5221 gataccactt tatcatcaag cgagacggta ctgtggaggc aggacgagat gagatggctg
     5281 taggctctca cgctaagggt tacaaccaca actctatcgg cgtctgcctt gttggtggta
     5341 tcgacgataa aggtaagttc gacgctaact ttacgccagc ccaaatgcaa tcccttcgct
     5401 cactgcttgt cacactgctg gctaagtacg aaggcgctgt gcttcgcgcc catcatgagg
     5461 tggcgccgaa ggcttcccct tcgttcgacc ttaagcgttg gtgggagaag aacgaactgg
     5521 tcacatctga ccgtggtagc ggcggtggtg cgagtggtgg tgcggacatg ggtaacgaaa
     5581 tcgacaccca gaaccgtcag atcgaccgta tcatggaaaa agctgacggc ggtagcgcag
     5641 gcagtggagc gggcggtaac acgattaaca tcgctaagaa cgacttctct gacatcgaac
     5701 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac
     5761 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg
     5821 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgctaagcct ctcatcacta
     5881 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc
     5941 gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt
     6001 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg
     6061 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg
     6121 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc
     6181 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac
     6241 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct
     6301 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg
     6361 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa
     6421 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta
     6481 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc
     6541 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg
     6601 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag
     6661 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg
     6721 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg
     6781 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta
     6841 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt
     6901 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag
     6961 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag
     7021 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc
     7081 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc
     7141 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct
     7201 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt
     7261 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg
     7321 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta
     7381 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta
     7441 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac
     7501 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga
     7561 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc
     7621 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat
     7681 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa
     7741 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg
     7801 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt
     7861 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc
     7921 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt
     7981 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag
     8041 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta
     8101 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat
     8161 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt
     8221 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct
     8281 tagagtcgga cttcgcgttc gcgtaatgga gattttcaac atgctccctc aatcggttga
     8341 atgtcgccct tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa
     8401 aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt
     8461 attttctacg tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg
     8521 ggtattccgt agaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
     8581 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     8641 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     8701 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     8761 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     8821 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     8881 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     8941 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     9001 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     9061 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     9121 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     9181 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     9241 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     9301 cgtgaaaggc gagatcacca aggtagtcgg caaa
//
