LOCUS       Exported                6543 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  X(4130)B1 expression vector.
ACCESSION   urn.local...1g-3js4sas
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      69..87
                     /standard_name="T7 Terminator"
                     /label=T7 Terminator
     misc_feature    complement(118..147)
                     /standard_name="MASP449"
                     /label=MASP449
                     /note="Geneious type: PCR_primer"
     CDS             complement(137..1474)
                     /standard_name="X(4130)B1"
                     /label=X(4130)B1
     misc_feature    137..181
                     /standard_name="TRB460"
                     /label=TRB460
                     /note="Geneious type: PCR_primer"
     misc_feature    139..157
                     /standard_name="TRB429"
                     /label=TRB429
                     /note="Geneious type: PCR_primer"
     misc_feature    159..1471
                     /standard_name="AACTTCACGATTTTCTCAACCAGGCCCTTGTCTGGGATGGAG 
                     TCAATGATTTTCGGATTCAGTTTGATGTTCTGACCACGATTATTCACGGCCAGATTGC 
                     CGATGTTGAAGCCTTCGCTGACGGTATAGATGTCATCGTCCAGCAGGTTCGGGACTTT 
                     CAGGAAGCCATATTTGATGAAGTAGGTATTACGACATTTGACTTTGAACTTGTTCGCC 
                     AGATCGATTTCGGTGAAGCTGTACAGTTTCTTGTAGATTTCATTGAATTTGTTTTCAT 
                     TCACGGTATAAGAACCATCCGCATTTTTGTCCAGGCCATATTTCCATTGAAAGTAGTC 
                     TTTGTACTCATTAATGTCGTATTCCGGCGGAGCGGAGTTAACACGAGACAAGCGCGTT 
                     GCAATCTTTTCATAGTTGGCCAGCAAATTGTTATAGATTTTCTCTTTCATGGCCGAGG 
                     TAATGATGTTCAAATCTTGACCGCCGAAGGTCAGGAACTCTTCCAGGCGAATCGGCTT 
                     TTCCGCAATCATCAGAGGTGCCTGCTTAACCTTGATAGTCTCTTTATACGTCACACCA 
                     CGGGCACCGTACAAGCCATGCAATGCATGGATCAGTTCGTGTGCCAGGCTGATCGCCG 
                     GGTCGGCGATGAAGCTCTCGGTAGAAGAATTGTAACCACCGCTGATATCATTGAAGGT 
                     GTACTCGTACTCCGGGCTAAACGTCACGATATTGATGCTGCCAAAACCGTCATTGCTC 
                     GGGTCATAAACACCGCCGCTATCCATCAGTTTACGCACCGGGTAGCTAGAGTTTTCGA 
                     AGATGTCCGGACCGGCACCCAGAACCAGCAGGTTCAGGATAATGCTAGACTTCACGTT 
                     GGTCGAGGACTTGATGTTAACGCTGGTAGTGCGGGTAACCGGGTGAAACTCGTTAATA 
                     GGCGTATGCTCATTACCCAGATAAGGCTTTGCGTAGCTAATCTCTTGCAGCAGGACCT 
                     CACCTGCCGGATTGCTGTTAATGCGTTTGAACAGCTTGATGGTGGTTTTCAAATAACG 
                     ATCTTTCTCCGCATCGGTGGTCAGGTAATTCGGGTCGTAATATGCGGACGAGCCGTTT 
                     TCCAAAGACGCAGGTGGATCGAAGTCGCTTGGGTCAGTACCAATCGTATTACGCTCCG 
                     GAATAATCCACACATTGCGCATGATTTCAAAGGCCTTATAGTACTTCTTGCTCTTCTC 
                     TTCATACGGGATTTGCATATACAGGATCGTGTCGTCGTTTACTGGGTCGTTGTAGTTG 
                     AAGCTGTTGATGACCACCGG"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     misc_difference complement(383..385)
                     /standard_name="L364I"
                     /label=L364I
     misc_difference complement(533..535)
                     /standard_name="Y314N"
                     /label=Y314N
     misc_difference complement(974..976)
                     /standard_name="T167I"
                     /label=T167I
     misc_difference complement(977..979)
                     /standard_name="A166E"
                     /label=A166E
     misc_difference complement(1031..1033)
                     /standard_name="N148T"
                     /label=N148T
     misc_difference complement(1046..1048)
                     /standard_name="N143D"
                     /label=N143D
     misc_feature    complement(1448..1489)
                     /standard_name="TRB461"
                     /label=TRB461
                     /note="Geneious type: PCR_primer"
     gene            1477..1518
                     /label=MSP883
                     /note="MSP883"
     promoter        complement(1541..1560)
                     /standard_name="T7 Promoter"
                     /label=T7 Promoter
     CDS             1950..3027
                     /standard_name="lacI"
                     /label=lacI
     unsure          2504..2509
     unsure          2745..2750
     rep_origin      complement(4444..5063)
                     /direction=LEFT
     misc_feature    5169..5984
                     /standard_name="KanR"
                     /label=KanR
                     /note="Geneious type: ORF"
                     /note="Geneious type: CDS"
     rep_origin      complement(6078..6533)
                     /direction=LEFT
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtggtt ttttgagttg cggtaaatgg
      181 cattatacaa caacccgtta cgtggacaaa ttttaataaa cgcacgaagt gcgttggact
      241 cgatcttttc aaaataagac gactctaaca gctggccttg gaaatcgttc gacccctggg
      301 agctgatatt aaaaccctct aaagtgctgt aactattgtc gtccaggatg tttacataga
      361 tagggtcgat tggacgctcc ttaatgtagt gcttgcgcac aaggatggag aaacgctgtg
      421 ccaagttaga ctcattaagt acaaacaaga tggtattaag tttcttctcg aactcagctg
      481 tatcaagttt gaagttaccc aaacgacctt gtactgggat tttgtttttg atatttttta
      541 acaaatcatt ctctacggta acagtattaa gacgctctga gatcagagta gtatagttgt
      601 ttttggccgt ttcgatgatc tttttgatga tcaatgatga aattgcttta gagtcaattc
      661 caccaaacgt aagaagctcc tcaaagataa gactgttttg ttgacgcgaa gtttcaatct
      721 ttcctgtgtc aaaattatag taaaagttgc gattgctgat gccgtaaaga ttatgagtca
      781 cgtgaaccag ctcgtgcatc aatgtcgaag caggatctgg agcgaactca cgttttacga
      841 atttatttac aatattcacc aaactacggt agttgccgta gctctcatcg aatggtttca
      901 agtagaatgg ggaaaacgac acttcactca gtgtcccttc accgttagag atcggcgtag
      961 aatacaatcc gtatatttca ttattcgcaa tatcggggcc gggcccataa atgaccagat
     1021 tagcttgcag ggtagacacg atgttgtcat tctcttgata cgcgatcgtc tcattgtccg
     1081 acagggttaa tgcgccgttg gagacaagag gcaatgggat agaggaggaa atcaactcta
     1141 aaagcttctc tccctcaggt ttgctcttga tacgctccag caccttgatt acaccttgca
     1201 agaactcgtc cttttcactc ggagtgttca ggtaattggg attatagata gcatccgcct
     1261 ccatgatagg ctctgacgga atgtttaaat cgtttgtgtt attcgtaaag ttgtaacgtt
     1321 ccggaacgat ccagatgttt ttaatcactt ggaaagcttt gaaaggcccc ttgcccttgt
     1381 tgattttatc agaatggcgg ggggggcgca tcgtgattac gttgatacca tcaatgggat
     1441 cgttgtagtt aaacttgtta atttcaagtt tcatgtatat ctccttctta aagttaaaca
     1501 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
     1561 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
     1621 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     1681 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     1741 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     1801 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     1861 agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg
     1921 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
     1981 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
     2041 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     2101 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     2161 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     2221 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     2281 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     2341 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     2401 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     2461 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
     2521 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
     2581 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     2641 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     2701 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     2761 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
     2821 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     2881 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     2941 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3001 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact
     3061 cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     3121 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     3181 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     3241 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     3301 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     3361 atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     3421 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     3481 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     3541 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     3601 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     3661 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     3721 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     3781 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     3841 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     3901 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     3961 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     4021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     4141 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4201 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4261 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4621 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4861 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5041 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5101 acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     5161 ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca
     5221 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     5281 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     5341 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     5401 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     5461 ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     5521 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     5581 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     5641 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     5701 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     5761 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     5821 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     5881 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     5941 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc
     6001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6061 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     6121 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     6181 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6241 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6301 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6361 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6421 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6481 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     6541 cca
//
