LOCUS       Exported                6459 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  BoNT/E LC expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     LOCUS pET28b 5368 bp DNA circular UNA 06-AUG-2014 DEFINITION . 
            ACCESSION urn.local...1g-3js4sas VERSION urn.local...1g-3js4sas 
            KEYWORDS . SOURCE ORGANISM .
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      69..87
                     /standard_name="T7 Terminator"
                     /label=T7 Terminator
     misc_feature    complement(118..147)
                     /label=MSP449
                     /note="MSP449"
     gene            complement(137..1390)
                     /standard_name="BoNT_E_LC"
                     /label=BoNT_E_LC
                     /note="BoNT_E_LC"
                     /note="Geneious type: CDS"
     misc_feature    complement(138..157)
                     /label=MSP422
                     /note="MSP422"
     misc_feature    138..157
                     /label=MSP423
                     /note="MSP423"
     gene            139..184
                     /standard_name="MSP892"
                     /label=MSP892
                     /note="MSP892"
                     /note="Geneious type: PCR_primer"
     misc_feature    139..157
                     /label=MSP448
                     /note="MSP448"
     gene            complement(1361..1405)
                     /standard_name="MSP893"
                     /label=MSP893
                     /note="MSP893"
                     /note="Geneious type: PCR_primer"
     gene            1393..1434
                     /standard_name="MSP883"
                     /label=MSP883
                     /note="MSP883"
                     /note="Geneious type: PCR_primer"
     misc_feature    complement(1457..1476)
                     /label=T7
                     /note="T7"
                     /note="Geneious type: promoter"
     CDS             1866..2945
                     /standard_name="LacI"
                     /label=LacI
     rep_origin      complement(4360..4979)
                     /direction=LEFT
     misc_feature    5085..5900
                     /standard_name="KanR"
                     /label=KanR
                     /note="Geneious type: CDS"
     rep_origin      complement(5994..6449)
                     /direction=LEFT
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgaaa ccggattatt ttcttcacta
      181 acccccgtcc tgtgatgggt ttaatgatgc gcggatttag gttggcgttt tggcctcgga
      241 aattcacctt taggttattt atattgtaac cctcactaat gttgtatatg gaatcattca
      301 ggagattcga cagcttgaaa tacttatact gtccgatgta tgtctctcgg catttgacct
      361 ggaatttggt ggccaagtcg aactccgtga agctgtaaag tttcttcaaa atatcgtcga
      421 atttgttaat gtttaccgaa tagattcctg acgcgtcttt gtctaatcca tatttctctt
      481 ggaatatgtc cttatacgga ttcagttgag ggtttgagac ttgaaccttg gataacttag
      541 aagcgatttt tctataatca tttagtaagt tggtatagat gtcattgtac tgtgcgacag
      601 ttataatgtt cagatcgttg cctccaaatg taagaaattc ctcgatgtta atgcccttcc
      661 tgttcgttat caatgggttc tgttgctgag taatgataca cgtagtggtt atccccttag
      721 cgccatacag gccgtgtaaa gagtgtatga gttcgtgcat aagcgtgagt gcggggtctt
      781 ggataaactc atttatacta ttatcattga agcgaaaact gtattcggga gaaaaagtaa
      841 cgatcgctat gctaccgaac ccatggtttg acggcatata gttatttctc aggctaatat
      901 tactcgaatt tgtttcaaat aaatctggtt ccgcccccat tataataacg ttgggcaaga
      961 gaatatgctg tgatccattg ctaaatttga tttccacggc ggatgcatcg cctatatgaa
     1021 actggttgtc tggtgtatta tcgttaccta ggtaaggatt agctttacta agctcttcaa
     1081 gcaaaatacc tccggataga ttgttattaa tcctgttaaa gattttggtt actattttaa
     1141 ggaatctgtc cttctcttca tccgactgga gataattggg gtcgtaatag gaagagtcac
     1201 cgttcttcaa cgaagtaggt ggatgaaaat cttgcggtgt cgtaccaata acgttacgct
     1261 caggtataat ccatatattc ttcataatat tgaaagattt gtagaactct tgacacccac
     1321 ccggctttat gtataggatg gtacgatcgt ttacagggtc attgtaatta aagctgttga
     1381 tttttggcat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
     1441 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
     1501 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     1561 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     1621 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     1681 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     1741 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc
     1801 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     1861 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     1921 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     1981 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     2041 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     2101 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     2161 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     2221 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     2281 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     2341 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     2401 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     2461 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     2521 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     2581 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     2641 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     2701 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     2761 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     2821 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     2881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2941 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg
     3001 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     3061 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     3121 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     3181 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     3241 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg
     3301 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
     3361 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
     3421 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
     3481 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
     3541 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
     3601 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
     3661 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
     3721 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
     3781 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
     3841 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
     3901 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3961 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4021 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     4081 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat
     4141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa
     5041 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     5101 gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     5161 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     5221 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     5281 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     5341 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
     5401 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     5461 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     5521 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     5581 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     5641 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     5701 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     5761 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     5821 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     5881 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag
     5941 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta
     6001 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     6061 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6121 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6181 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6241 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6301 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6361 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6421 gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca
//
