LOCUS       Exported                6936 bp ds-DNA     circular SYN 01-MAR-2021
DEFINITION  VAMP8 FRET Kinetics substrate.
ACCESSION   urn.local...1g-3js4sas
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 2, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="synthetic DNA construct"
     source          137..157
     source          158..178
     source          158..178
     source          158..178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          179..904
     source          179..874
     source          179..874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          875..904
     source          875..904
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          905..922
     source          905..922
     source          923..1105
     source          923..1105
     source          1106..1120
     source          1106..1120
     source          1121..1834
     source          1121..1834
     source          1835..1867
     source          1835..1867
     source          1835..1864
     source          1865..1867
     source          1865..1867
     misc_feature    69..87
                     /standard_name="T7 terminator"
                     /label=T7 terminator
                     /note="t7 term"
                     /note="Geneious type: terminator"
     CDS             complement(137..1867)
                     /standard_name="CVm-VAMP8"
                     /label=CVm-VAMP8
     misc_feature    complement(137..874)
                     /standard_name="mVenus"
                     /label=mVenus
     primer_bind     complement(200..221)
                     /standard_name="EGFP-C"
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     549..568
                     /standard_name="EXFP-R"
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     808..829
                     /standard_name="EGFP-N"
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(881..904)
                     /codon_start=1
                     /standard_name="Xpress(TM) tag"
                     /product="Xpress(TM) epitope tag, including anenterokinase 
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /note="Geneious type: misc_feature"
                     /translation="DLYDDDDK"
     misc_feature    complement(923..1105)
                     /standard_name="VAMP8 (G7–R67)"
                     /label=VAMP8 (G7–R67)
     misc_feature    complement(1121..1834)
                     /standard_name="eCFP"
                     /label=eCFP
     misc_feature    complement(1835..1867)
                     /standard_name="c-Myc Tag"
                     /label=c-Myc Tag
     misc_feature    complement(1936..1953)
                     /standard_name="T7 promoter"
                     /label=T7 promoter
                     /note="t7 pro"
                     /note="Geneious type: promoter"
     CDS             2343..3422
                     /standard_name="LacI"
                     /label=LacI
     unsure          2897..2902
     unsure          3138..3143
     rep_origin      complement(4837..5456)
                     /direction=LEFT
     CDS             5562..6377
                     /standard_name="KanR"
                     /label=KanR
     rep_origin      complement(6471..6926)
                     /direction=LEFT
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctt gtacagctcg tccatgccga
      181 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      241 ggtctttgct cagggcggac tggtagctca ggtagtggtt gtcgggcagc agcacggggc
      301 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg ccgtcctcga
      361 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat
      421 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      481 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      541 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      601 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
      661 agcactgcag gccgtagccc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      721 cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      781 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      841 ccccggtgaa cagctcctcg cccttgctca ccatcggatc cttatcgtca tcgtcgtaca
      901 gatcacttcc accactattt gaacgcgcaa ccttttgact ggtagtctta aaatgctctg
      961 aagtggcctc taagtcttcg gttttattgc gcagatgctc aaggttctcg ccacgggcca
     1021 aaatacgctc tacattttgg gtcatgatat ttttcacgcc ttcgacctct gattgtaagt
     1081 tacgaacgcg gtcgttacca cctccacgac tacgcccacc tttatacagt tcatccatac
     1141 catgagtaat accagcggcg gtaacgaact ctaaaaggac catatggtcg cgtttctcat
     1201 tggggtcctt agacagggct gactgtgtgc tcaaatagtg attatcaggt aaaaggacgg
     1261 ggccatctcc aattggtgtg ttttgctggt aatggtctgc aagctggacg cttccatcct
     1321 caatgttgtg acgaatcttg aaatttgcct tgattccgtt tttttgttta tcagccgtga
     1381 tgtagacgtt atgagagata tagttgtatt ctaatttatg tcctaagatg ttgccatcct
     1441 ctttaaagtc gatacccttc aactcaatgc gattaacaag agtatctccc tcaaacttaa
     1501 cctcggcgcg ggtcttatag tttccatcat ctttaaaaaa aatggtgcgt tcctggacgt
     1561 aaccttctgg catcgcggac ttgaaaaagt cgtgttgttt catatggtca gggtagcgag
     1621 cgaaacactg cacgccccag gcgaacgtag taaccaatgt aggccacggt actggaagct
     1681 tgccggtagt gcaaataaat tttaatgtca actttccgta cgtcgcatcg ccctctcctt
     1741 ccccagatac ggaaaacttg tgaccattca catcgccatc cagttctaca agaatgggga
     1801 ccacgccggt aaacaattcc tcccccttag acatcaagtc ttcctcagag ataagttttt
     1861 gttccatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
     1921 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
     1981 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     2041 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     2101 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     2161 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     2221 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca
     2281 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     2341 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     2401 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     2461 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     2521 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     2581 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     2641 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     2701 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     2761 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     2821 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     2881 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     2941 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     3001 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     3061 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     3121 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     3181 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     3241 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     3301 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     3361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3421 gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg
     3481 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3541 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3601 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3661 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3721 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc
     3781 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
     3841 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
     3901 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
     3961 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
     4021 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
     4081 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
     4141 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
     4201 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
     4261 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
     4321 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
     4381 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4441 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4501 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4561 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg
     4621 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     4681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5281 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa
     5521 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     5581 aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     5641 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
     5701 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     5761 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     5821 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat
     5881 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     5941 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     6001 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     6061 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     6121 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     6181 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     6241 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     6301 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     6361 tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa
     6421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac
     6481 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     6541 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     6601 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     6661 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     6721 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     6781 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     6841 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     6901 cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//
