LOCUS       Exported                5634 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pFA6a-link-yomCherry-CaURA3
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1019..1035
                     /locus_tag="SP6 promotor"
                     /label=SP6 promotor
     misc_feature    1082..1096
                     /locus_tag="annealing primer for homology 
                     recombinationtagging"
                     /label=annealing primer for homology
                     recombination tag...
                     /label=annealing primer for homology recombinationtagging
     misc_feature    1106..2536
                     /locus_tag="tdTomato"
                     /label=tdTomato
     misc_feature    2839..3182
                     /locus_tag="tef promotor"
                     /label=tef promotor
     misc_feature    3183..3994
                     /locus_tag="Ura3"
                     /label=Ura3
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatggt tagtaaaggt gaggaagtga ttaaggaatt
     1141 catgaggttt aaagttagaa tggaaggttc tatgaatggt catgaatttg aaattgaagg
     1201 tgaaggtgaa ggtagaccat atgaaggtac acaaactgct aaattgaaag ttacaaaagg
     1261 tggtccatta ccatttgcat gggatatttt atctccacag tttatgtatg gtagtaaagc
     1321 atatgttaaa catccagctg atattccaga ttataagaaa ttaagttttc ctgaaggttt
     1381 taaatgggaa agagtaatga attttgaaga tggtggttta gtaacagtta ctcaagattc
     1441 ttctttacaa gatggtacat taatatataa agttaaaatg agaggtacta attttccacc
     1501 tgatggtcca gttatgcaga agaaaacaat gggttgggaa gcctcaactg aaagattgta
     1561 ccctagagat ggagttttga aaggtgaaat tcatcaagct cttaagttaa aagatggagg
     1621 tcactatttg gttgagttta aaactatata catggctaaa aaacctgttc aattgccagg
     1681 ttattattat gttgatacta aattagatat tacttcacat aatgaagatt atactattgt
     1741 tgaacaatat gaaagatctg aaggtagaca tcatttgttt ttgggtcatg gtactggtag
     1801 tactggttct ggttcttcag gtactgcttc tagtgaagat aataatatgg ctgtaatcaa
     1861 agagttcatg aggtttaaag ttagaatgga aggttctatg aatggtcatg agtttgaaat
     1921 cgaaggtgaa ggagaaggta gaccatatga aggtactcaa actgctaaat tgaaagttac
     1981 taaaggtggt ccattgccat ttgcttggga tatattgtca cctcagttta tgtatggttc
     2041 taaagcatat gttaaacatc cagctgatat tcctgattat aaaaaattat catttcctga
     2101 aggttttaaa tgggaaagag ttatgaattt tgaagatggt ggtttagtta ctgtaactca
     2161 agattcttca ttgcaagatg gtacattaat atataaagtt aaaatgagag gtactaattt
     2221 tccacctgat ggtccagtta tgcagaaaaa gactatgggt tgggaagcat ctactgaaag
     2281 attatatcct agagatggtg ttttaaaagg tgaaattcac caagccttaa aattgaaaga
     2341 tggtggtcat tatttggttg aattcaaaac aatctacatg gctaaaaaac cagttcagct
     2401 gcccggatac tattatgtcg atactaaact ggatattact tcacacaatg aagactacac
     2461 aattgttgaa cagtatgaaa gatcagaggg tagacatcac ttgtttttat acggtatgga
     2521 tgaattatat aaataagcgg ccgcctcgag gtcgacggcg cgccacttct aaataagcga
     2581 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     2641 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     2701 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta
     2761 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg
     2821 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     2881 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     2941 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     3001 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     3061 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     3121 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     3181 ccatgacagt caacactaag acctatagtg agagagcaga aactcatgcc tcaccagtag
     3241 cacagcgatt atttcgatta atggaactga agaaaaccaa tttatgtgca tcaattgacg
     3301 ttgataccac taaggaattc cttgaattaa ttgataaatt aggtccttat gtatgcttaa
     3361 tcaagactca tattgatata atcaatgatt tttcctatga atccactatt gaaccattat
     3421 tagaactttc acgtaaacat caatttatga tttttgaaga tagaaaattt gctgatattg
     3481 gtaataccgt aaagaaacaa tatattggtg gagtttataa aattagtagt tgggcagata
     3541 ttaccaatgc tcatggtgtc actgggaatg gagtggttga aggattaaaa cagggagcta
     3601 aagaaaccac caccaaccaa gagccaagag ggttattgat gttagctgaa ttatcatcag
     3661 tgggatcatt agcatatgga gaatattctc aaaaaactgt tgaaattgct aaatccgata
     3721 aggaatttgt tattggattt attgcccaac gtgatatggg tggccaagaa gaaggatttg
     3781 attggcttat tatgacacct ggagttggat tagatgataa aggtgatgga ttaggacaac
     3841 aatatagaac tgttgatgaa gttgttagca ctggaactga tattatcatt gttggtagag
     3901 gattgtttgg taaaggaaga gatccagata ttgaaggtaa aaggtataga aatgctggtt
     3961 ggaatgctta tttgaaaaag actggccaat tataatcagt actgacaata aaaagattct
     4021 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     4081 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     4141 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     4201 gtcgattcga tactaacgcc gccatccagt gtcgaaaacg agctcgaatt catcgatgat
     4261 atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt
     4321 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     4381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4741 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     4801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4981 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gttt
//
