LOCUS       Exported                8433 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    851..1567
                     /locus_tag="yeast optimized mTq2d9 from DBO159"
                     /label=yeast optimized mTq2d9 from DBO159
     misc_feature    1568..1603
                     /locus_tag="high FRET linker"
                     /label=high FRET linker
     misc_feature    1604..2314
                     /locus_tag="yomCherry"
                     /label=yomCherry
     misc_feature    4688..5487
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5919..6779
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7862..7891
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc actagtaagc
      841 ttttaattaa atggttagta aaggtgaaga attgttcact ggtgtagtgc caattttagt
      901 agaattagat ggtgacgtta acggtcataa gttctctgta agcggtgaag gtgaaggtga
      961 cgcaacatac ggtaaattaa ctttgaaatt catttgtact acaggtaaat taccagttcc
     1021 atggccaact ttggttacta ctttgtcttg gggtgttcaa tgtttcgcta gatacccaga
     1081 tcatatgaag caacatgatt tctttaagtc tgctatgcca gaaggttacg ttcaagaaag
     1141 aactatcttc ttcaaggatg atggtaacta taagactaga gctgaagtta agttcgaagg
     1201 agatacttta gttaatagaa ttgaattgaa gggtattgat tttaaggaag atggtaatat
     1261 tttgggtcat aagttggaat ataattactt ctctgataac gtttacatta ctgctgataa
     1321 gcaaaagaac ggtattaagg cgaacttcaa gattagacat aacattgaag atggtggtgt
     1381 tcaattagct gatcattacc aacaaaacac tccaattggt gacggtccag ttttgttgcc
     1441 agataaccat tacttgtcta ctcaatctaa gttgtctaag gatccaaacg aaaagagaga
     1501 tcatatggtt ttgttggaat tcgttactgc tgctgggatc actttaggta tggatgaatt
     1561 gtataaatcc ggactcagat cttcggatcc accggtcgcc accatggtta gcaaaggcga
     1621 ggaagataac atggctataa tcaaagagtt tatgagattc aaagtacaca tggagggttc
     1681 agtgaatggt catgaatttg aaattgaagg cgaaggcgag ggcagacctt acgaaggaac
     1741 tcaaacagca aaacttaagg taacaaaagg tggtcctctg ccattcgcct gggacattct
     1801 cagtccacaa ttcatgtacg gttctaaagc gtacgtcaaa catccagcag acattccaga
     1861 ttacttgaaa ttgtcttttc cagaaggctt taagtgggaa agagttatga acttcgagga
     1921 tggaggggtt gtgaccgtta cgcaagattc ctctttacaa gatggtgagt ttatctacaa
     1981 ggtcaaatta agggggacta attttccttc agacgggcca gtcatgcaga aaaagactat
     2041 gggatgggaa gcctcttcag agagaatgta tcctgaagat ggcgctctaa aaggagaaat
     2101 caagcaaaga ttgaagttaa aggacggagg tcattatgat gcagaagtaa aaacaaccta
     2161 taaagctaaa aagccagttc aacttcctgg tgcctacaat gttaacatca agctagacat
     2221 tacatcccat aatgaagatt acactatagt ggaacagtat gaacgtgctg aaggtagaca
     2281 cagtacaggt ggtatggatg aactgtacaa gtaagcggcc gcacccagct ttcttgtaca
     2341 aagtggtgta gttaattcat gatggccgct gcaggtcgac ctcgaggggg ggcccggtac
     2401 ccaattcgcc ctatagtgag tcgtattacg cgcggatcca gctttggact tcttcgccag
     2461 aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga
     2521 aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa
     2581 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     2641 attttaaagt gactcttagg ttttaaaacg anaattctta ttcttgagta actctttcct
     2701 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac
     2761 cggcatgcca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     2821 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     2881 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     2941 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataatcgg atcgtacttg
     3001 ttacccatca ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat
     3061 atttgaacct gtataataat atatagtcta gcgctttacg gaagacaatg tatgtatttc
     3121 ggttcctgga gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt
     3181 tcattttctg cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt
     3241 gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     3301 gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat
     3361 ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     3421 aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa
     3481 agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac
     3541 aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa
     3601 ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct
     3661 tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt
     3721 gcattttttc aagataaagg catccccgat tatattctat accgatgtgg attgcgcata
     3781 ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg
     3841 tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt
     3901 tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag
     3961 ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat
     4021 gggtaggtta tatagggata tagcacagag atatatagca aagagatact tttgagcaat
     4081 gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg
     4141 ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat
     4201 actttctagc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
     4261 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta
     4321 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
     4381 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
     4441 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
     4501 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
     4561 ttgatattgg atcgatccga tgataagctg tcaaacatga gaattgggta ataactgata
     4621 taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
     4681 tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
     4741 ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
     4801 gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc
     4861 cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
     4921 acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc
     4981 aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     5041 catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     5101 aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta
     5161 tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc
     5221 gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     5281 ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     5341 ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     5401 ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     5461 acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg tttttgttct
     5521 gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat
     5581 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
     5641 atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
     5701 gaaaagctaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5761 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6121 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6301 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6781 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6841 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7081 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7561 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7621 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7681 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7741 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7801 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7861 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7921 acacaggaaa cagctatgac catgattacg ccaagcttac cgcatcagga aattgtaagc
     7981 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     8041 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     8101 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     8161 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     8221 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     8281 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     8341 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     8401 cttaatgcgc cgctacaggg cgcgtccatt cgc
//
