LOCUS       Exported                8436 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1526
                     /locus_tag="AuusFP1"
                     /label=AuusFP1
     misc_feature    1533..1607
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1608..2318
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4691..5490
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5922..6782
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7865..7894
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atgagttacg
      841 gagcactttt gttcagagaa aaaatcccct atgttgttga gatggaaggt gatgttgagg
      901 gaatgaagtt ttcagtaaga ggtaaaggac atggtgatgc taacactggt aaaattgaag
      961 ccagtttcat ctgcacaact ggtgaactac cagtaccttg gtcaagcatt ctaacaactg
     1021 tcacatacgg tgcacagtgt tttgcaaaat atccaaatga tatcaaagac tacccaaaaa
     1081 gtgctatgcc agaaggttac gttcaagagc gaacaatcac ttttgaaaac gacggcgttt
     1141 ataagacacg cgctgaagtc acgtacgaga aaggatcagt ttataaccga gttacgttga
     1201 acggttcagg atttaaaaaa ggcggtaaca tcttgggcaa aaaattggaa tttaactaca
     1261 accctcattg catctatgtt cttccagatg tacaaaataa tggaatcaaa tgctacatca
     1321 acattgttca tgacgtaatt ggaggaggac aaataattgc tgcgcaccaa caattaaata
     1381 ctcctcttgg cggtggacca gtcgacattc cacactacca tcatatacaa gcgcacacaa
     1441 tactttctaa agatcccaaa gaaacaagag atcatatgaa cgtagtagag gtgttcagag
     1501 ctatcgattg caaaaccgca tatgcatccg gagagggcag aggaagtctt ctaacatgcg
     1561 gtgacgtgga ggagaatccc ggccctgggg atccaccggt cgccaccatg gtgagcaagg
     1621 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1681 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1741 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1801 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1861 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1921 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     1981 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     2041 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     2101 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     2161 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     2221 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     2281 gccactccac cggcggcatg gacgagctgt acaagtagcg gccgcaccca gctttcttgt
     2341 acaaagtggt gtagttaatt catgatggcc gctgcaggtc gacctcgagg gggggcccgg
     2401 tacccaattc gccctatagt gagtcgtatt acgcgcggat ccagctttgg acttcttcgc
     2461 cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta
     2521 cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc
     2581 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     2641 caaattttaa agtgactctt aggttttaaa acganaattc ttattcttga gtaactcttt
     2701 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     2761 taccggcatg ccaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2821 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2881 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     2941 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataat cggatcgtac
     3001 ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag
     3061 tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat
     3121 ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc
     3181 ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc
     3241 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     3301 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     3361 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     3421 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     3481 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     3541 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     3601 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     3661 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     3721 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     3781 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     3841 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     3901 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     3961 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     4021 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     4081 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     4141 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     4201 tatactttct agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa
     4261 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac
     4321 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt
     4381 atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct
     4441 cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta
     4501 gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt
     4561 cctttgatat tggatcgatc cgatgataag ctgtcaaaca tgagaattgg gtaataactg
     4621 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     4681 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     4741 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     4801 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     4861 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
     4921 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
     4981 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
     5041 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
     5101 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
     5161 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
     5221 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
     5281 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
     5341 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
     5401 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
     5461 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc atgtttttgt
     5521 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     5581 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     5641 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     5701 attgaaaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5761 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5821 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5881 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5941 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6001 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6061 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6121 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6181 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6241 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6301 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6361 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6421 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6481 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6541 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6601 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6661 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6721 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6781 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6841 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6961 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7021 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7081 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7141 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7201 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7261 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7321 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7381 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7441 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7501 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7561 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7621 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7681 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7741 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7801 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7861 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7921 ttcacacagg aaacagctat gaccatgatt acgccaagct taccgcatca ggaaattgta
     7981 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     8041 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     8101 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     8161 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     8221 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     8281 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     8341 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     8401 gcgcttaatg cgccgctaca gggcgcgtcc attcgc
//
