LOCUS       Exported                7866 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     misc_feature    893..979
                     /locus_tag="Cyterm 29 aminoacids Rabbit cytochrome 
                     p450sequenc"
                     /label=Cyterm 29 aminoacids Rabbit cytochrome
                     p450 seq...
                     /label=Cyterm 29 aminoacids Rabbit cytochrome p450sequenc
     misc_feature    980..1007
                     /locus_tag="linker"
                     /label=linker
     misc_feature    1031..1634
                     /locus_tag="yemVenus"
                     /label=yemVenus
     misc_feature    1635..1645
                     /locus_tag="yemVenus(2)"
                     /label=yemVenus
                     /label=yemVenus(2)
     misc_feature    1646..1648
                     /locus_tag="yemVenus(3)"
                     /label=yemVenus
                     /label=yemVenus(3)
     misc_feature    1646..1648
                     /locus_tag="a206k"
                     /label=a206k
     misc_feature    1649..1747
                     /locus_tag="yemVenus(1)"
                     /label=yemVenus
                     /label=yemVenus(1)
     misc_feature    1649..1724
                     /locus_tag="yeVenus"
                     /label=yeVenus
     misc_feature    4121..4920
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5352..6212
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7295..7324
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cgctaccgga
      841 ctcagatctg gtaccccttg cagcttccag ttgtcaggaa gagaaggctc ccatggatcc
      901 tgtggtggtg ctggggctct gtctctcctg tttgcttctc ctttcactct ggaaacagag
      961 ctatggggga gggaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1021 ggtcgccacc atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
     1081 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
     1141 tacttacggt aaattgacct taaaattgat ttgtactact ggtaaattgc cagttccatg
     1201 gccaacctta gtcactactt taggttatgg tttgcaatgt tttgctagat acccagatca
     1261 tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
     1321 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
     1381 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt
     1441 aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcactg ctgacaaaca
     1501 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
     1561 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
     1621 caaccattac ttatcctatc aatctaaatt atccaaagat ccaaacgaaa agagagacca
     1681 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
     1741 caaataagcg gccgcaccca gctttcttgt acaaagtggt gtagttaatt catgatggcc
     1801 gctgcaggtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
     1861 acgcgcggat ccagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt
     1921 tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg
     1981 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat
     2041 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     2101 acganaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     2161 gcatgaggtc gctcttattg accacacctc taccggcatg ccaattcact ggccgtcgtt
     2221 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2281 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2341 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     2401 ggtatttcac accgcataat cggatcgtac ttgttaccca tcattgaatt ttgaacatcc
     2461 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
     2521 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
     2581 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
     2641 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     2701 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     2761 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     2821 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     2881 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     2941 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     3001 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     3061 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     3121 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     3181 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     3241 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     3301 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     3361 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     3421 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     3481 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     3541 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     3601 ttttggtttt caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc
     3661 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
     3721 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
     3781 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
     3841 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
     3901 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
     3961 gattagtctc atccttcaat gctatcattt cctttgatat tggatcgatc cgatgataag
     4021 ctgtcaaaca tgagaattgg gtaataactg atataattaa attgaagctc taatttgtga
     4081 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     4141 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     4201 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     4261 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     4321 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     4381 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     4441 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
     4501 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
     4561 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac
     4621 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga
     4681 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg
     4741 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
     4801 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
     4861 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat
     4921 gatttatctt cgtttcctgc atgtttttgt tctgtgcagt tgggttaaga atactgggca
     4981 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
     5041 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa
     5101 tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc taattcttga agacgaaagg
     5161 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5221 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5281 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5341 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5401 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5461 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5521 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5581 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5641 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5701 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5761 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5821 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5881 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5941 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6001 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6061 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6121 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6181 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6241 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6301 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7021 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7081 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7141 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7201 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7261 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7321 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7381 acgccaagct taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     7441 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     7501 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     7561 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     7621 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     7681 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     7741 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     7801 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     7861 attcgc
//
