LOCUS       Exported                7848 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     misc_feature    893..908
                     /locus_tag="Cyterm 29 aminoacids Rabbit cytochrome 
                     p450sequenc"
                     /label=Cyterm 29 aminoacids Rabbit cytochrome
                     p450 seq...
                     /label=Cyterm 29 aminoacids Rabbit cytochrome p450sequenc
     misc_feature    909..979
                     /locus_tag="Cyterm 29 aminoacids Rabbit cytochrome 
                     p450sequenc(1)"
                     /label=Cyterm 29 aminoacids Rabbit cytochrome
                     p450 seq...
                     /label=Cyterm 29 aminoacids Rabbit cytochrome 
                     p450sequenc(1)
     misc_feature    980..1007
                     /locus_tag="linker"
                     /label=linker
     misc_feature    1031..1729
                     /locus_tag="ymScarletI"
                     /label=ymScarletI
     misc_feature    4103..4902
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5334..6194
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7277..7306
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cgctaccgga
      841 ctcagatctg gtaccccttg cagcttccag ttgtcaggaa gagaaggctc ccatggatcc
      901 tgtggtggtg ctggggctct gtctctcctg tttgcttctc ctttcactct ggaaacagag
      961 ctatggggga gggaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1021 ggtcgccacc atggttagta aaggtgaagc tgttataaaa gaattcatga ggtttaaagt
     1081 tcatatggaa ggttcaatga atggtcatga atttgaaatt gaaggtgaag gtgaaggtag
     1141 accatatgaa ggtacacaaa ctgctaaatt gaaagttact aaaggtggtc cattaccatt
     1201 ttcttgggat attttgtctc cacaattcat gtatggttct agagctttta ttaagcatcc
     1261 agctgatatt ccagattatt ataaacaatc ttttcctgaa ggttttaaat gggaaagagt
     1321 tatgaatttc gaagatggtg gtgctgttac tgttactcaa gatacttctt tggaagatgg
     1381 tactttaatc tataaagtta aattgagagg tactaatttt ccaccagatg gtccagttat
     1441 gcaaaagaaa actatgggtt gggaagcatc tactgaaaga ttgtatccag aagatggtgt
     1501 tttgaaagga gacattaaga tggctttgag attgaaagat ggtggtcggt acctggccga
     1561 cttcaagacc acctataaag ctaaaaaacc agttcaaatg ccaggtgcat ataatgttga
     1621 tagaaagtta gatataacgt cgcataacga ggactatact gtagttgaac aatatgaacg
     1681 tagtgaaggt agacatagta ccggaggaat ggatgaattg tataaataag cggccgcacc
     1741 cagctttctt gtacaaagtg gtgtagttaa ttcatgatgg ccgctgcagg tcgacctcga
     1801 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt
     1861 ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt
     1921 gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac
     1981 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     2041 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacganaat tcttattctt
     2101 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     2161 tgaccacacc tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg
     2221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2341 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2401 atcggatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     2461 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     2521 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     2581 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     2641 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     2701 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     2761 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     2821 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     2881 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     2941 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     3001 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     3061 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     3121 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     3181 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     3241 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     3301 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     3361 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     3421 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     3481 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     3541 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     3601 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     3661 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     3721 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     3781 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     3841 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     3901 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     3961 atgctatcat ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt
     4021 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     4081 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     4141 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     4201 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     4261 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     4321 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     4381 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     4441 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     4501 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     4561 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     4621 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     4681 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     4741 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     4801 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     4861 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     4921 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     4981 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     5041 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
     5101 aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     5161 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5521 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5581 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5641 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5701 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5761 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5821 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5881 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5941 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6001 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6061 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6121 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6181 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6241 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6481 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7021 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7081 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7141 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7201 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7261 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7321 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttaccgcat
     7381 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7441 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7501 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7561 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7621 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     7681 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7741 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7801 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgc
//
