LOCUS       Exported                7763 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    873..1643
                     /locus_tag="lifeact-mscarlet"
                     /label=lifeact-mscarlet
     misc_feature    945..1643
                     /locus_tag="mscarlet"
                     /label=mscarlet
     misc_feature    4018..4817
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5249..6109
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7192..7221
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggac
      841 tcagatctcg agctcaagct tcgaattcca ccatgggcgt ggccgacctg atcaagaagt
      901 tcgagagcat cagcaaggag gagggggatc caccggtcgc caccatggtg agcaagggcg
      961 aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc
     1021 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1081 agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt
     1141 tcatgtacgg ctccagggcc ttcaccaagc accccgccga catccccgac tactataagc
     1201 agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg
     1261 tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc
     1321 gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag
     1381 catccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc
     1441 tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga
     1501 agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca
     1561 acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg
     1621 gcatggacga gctgtacaag taaagcggcc gcacccagct ttcttgtaca aagtggtgta
     1681 gttaattcat gatggccgct gcaggtcgac ctcgaggggg ggcccggtac ccaattcgcc
     1741 ctatagtgag tcgtattacg cgcggatcca gctttggact tcttcgccag aggtttggtc
     1801 aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa
     1861 aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga
     1921 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     1981 gactcttagg ttttaaaacg anaattctta ttcttgagta actctttcct gtaggtcagg
     2041 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcca
     2101 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2161 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2221 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2281 tccttacgca tctgtgcggt atttcacacc gcataatcgg atcgtacttg ttacccatca
     2341 ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct
     2401 gtataataat atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga
     2461 gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg
     2521 cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt
     2581 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     2641 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     2701 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     2761 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     2821 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     2881 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     2941 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     3001 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     3061 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     3121 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     3181 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     3241 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     3301 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     3361 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     3421 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     3481 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc
     3541 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
     3601 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
     3661 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
     3721 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
     3781 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
     3841 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
     3901 atcgatccga tgataagctg tcaaacatga gaattgggta ataactgata taattaaatt
     3961 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
     4021 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
     4081 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
     4141 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
     4201 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
     4261 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
     4321 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
     4381 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
     4441 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
     4501 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
     4561 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
     4621 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
     4681 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
     4741 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
     4801 tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg
     4861 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
     4921 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
     4981 tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctaa
     5041 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5101 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5401 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5461 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5521 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5581 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5641 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5701 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5761 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5821 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5881 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5941 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6001 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6061 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7081 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7141 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7201 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7261 cagctatgac catgattacg ccaagcttac cgcatcagga aattgtaagc gttaatattt
     7321 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7381 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7441 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7501 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     7561 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     7621 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     7681 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     7741 cgctacaggg cgcgtccatt cgc
//
