LOCUS       Exported                7669 bp ds-DNA     circular SYN 03-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 3, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     gene            831..1550
                     /locus_tag="Geneious name: mGreenLantern"
                     /label=Geneious name: mGreenLantern
                     /note="Geneious name: mGreenLantern"
     misc_feature    3924..4723
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5155..6015
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7098..7127
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccttaggcta cggcgtggcc tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg
     1141 acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgtgctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaacttcaa cagccacaag gtctatatca cggccgacaa gcagaagaac ggcatcaagg
     1321 ctaacttcaa gacccgccac aacgttgagg acggcggcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc
     1441 atcagtccaa actgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgaaggaga
     1501 gggtgaccgc cgccgggatt acacatgaca tggacgagct gtacaagtaa gcggccgcac
     1561 ccagctttct tgtacaaagt ggtgtagtta attcatgatg gccgctgcag gtcgacctcg
     1621 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg gatccagctt
     1681 tggacttctt cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct
     1741 tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca
     1801 cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     1861 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacganaa ttcttattct
     1921 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta
     1981 ttgaccacac ctctaccggc atgccaattc actggccgtc gttttacaac gtcgtgactg
     2041 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2101 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2161 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2221 aatcggatcg tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc
     2281 ctgaaacaga tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag
     2341 acaatgtatg tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc
     2401 acgtcgcatc cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg
     2461 ttaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt
     2521 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat
     2581 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct
     2641 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc
     2701 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga
     2761 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa
     2821 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt
     2881 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac
     2941 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg
     3001 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc
     3061 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta
     3121 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta
     3181 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa
     3241 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga
     3301 gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag
     3361 tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc
     3421 gctctgaagt tcctatactt tctagctaga gaataggaac ttcggaatag gaacttcaaa
     3481 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     3541 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     3601 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     3661 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     3721 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     3781 aatgctatca tttcctttga tattggatcg atccgatgat aagctgtcaa acatgagaat
     3841 tgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     3901 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
     3961 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     4021 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     4081 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     4141 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     4201 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     4261 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     4321 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     4381 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     4441 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     4501 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     4561 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     4621 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     4681 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     4741 tgcatgtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     4801 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     4861 gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa
     4921 aaaaaaatga tgaattgaaa agctaattct tgaagacgaa agggcctcgt gatacgccta
     4981 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6961 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7141 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttaccgca
     7201 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7261 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7321 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7381 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7441 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7501 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7561 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7621 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgc
//
