LOCUS       Exported               11096 bp ds-DNA     circular SYN 06-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             71..889
                     /codon_start=1
                     /label=AvTnsA
                     /translation="MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSS
                     RTPGWKTNRVHHFFSDHEKRLFYLFEWSDIVTDIREQFPLDNLDLAMSIATDIGIKYPV
                     DTQSGTPHVLTSDFMLTVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAKGID
                     WGIITEKEIPRLLAENVEWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVT
                     TALDKEMNINSGTSLYVFKHLIARKEILVDLVAIKLSACPSTKVIEKIIF"
     CDS             912..3140
                     /codon_start=1
                     /label=AvTnsB
                     /translation="MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKG
                     LPEPRKISDILEAITEGIAFKQQQDPWARIIRDENLTDKEKEYRDKAWEIISSLIIEEP
                     SIYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFINSSG
                     KGKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYE
                     LMVKEYYKEGIRYDENGVQKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRV
                     ALENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNRDWIIGRPVIYVIIDVFSRMI
                     TGVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELA
                     GMTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARD
                     YRLDGKLDIDQFTEIIIRLIVHHNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSG
                     RLRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKNEQWFEKARSNLLSKSE
                     KSLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKS
                     QGKQLQEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEG
                     FELAITDNQSSFQVAESTAETSEQPESLQPNHLNLLKKKRQERKRGQN"
     CDS             3127..4797
                     /codon_start=1
                     /label=AvTnsC
                     /translation="VDRIESELVKIPNGELAVIARYIDQKLPEYNTNPLIQALPPILSA
                     AEFIDKVAREPNFDEQERELEPHYRFHCIERLSRYFDPQNKTLELQKVICTLIMTGYLS
                     RNLLKPEYANRSRQIYNAIGNGGGKNLENYVNLPTSASGLTLIGPSGIGKTTNFKNILE
                     LYPQIIFHPDYSVYQIVWLRVDCPKTLKGLCIDTFAGIDKLLGTNLLKKFNPARTSEDY
                     LLAQLAQTAHTHHLGVLVIDEIQNLATAKRNRDEILNFLVKLDNTIGIPVIRVGTNEAI
                     SFLQGNLRNARRNTGEGSRMWDRMVNNDEWCYFLEGIWEYQWTKTPVHFSDEIKEVFYD
                     ESQGIIDIAIKLYKMVQWRAISIGDQEIITVDLIRQVAKEGLYLVKPMLDAIRLGDREW
                     MIKYKDIAPLDITEYHNRCVSEIDAKDLTEIKRLAKKQQNQQKVSPKLNYVISELLKLE
                     VESGKAKECAEQIVYSHEDPDIPIPSLVNKAYALALQGCQVNESLAKTSTRKAKLKTKY
                     QENDIRRIVENAKKDKKSAYEDLKLAGIIKNPVEDFINI"
     CDS             4805..5755
                     /codon_start=1
                     /label=AvTniQ
                     /translation="MLSFFPTLYPDELLYSTLARYHIRSGNKSFKQTDLELFGYSSQQI
                     CKITLTNNLNYLVKNLPLRSQQKVENLLQNHTLYSFYATLLMPQEAWLLKNSMSNKFSG
                     SILDFAKVANNSSDDSKKFLKFCPECLEKDTQKYGEPYWHRIHQVPGILVCPIHRVVLH
                     NSCIEVESKGIHYHAASIDNCLLANNVKKLTNKTVETLSILAHDINWLIHSSFAFQGLA
                     WLRAQYQRYLLDKGLLKTFAGGKFKFNDCGFAQLIFEFYGQESLEALNPKLTQNQGKYF
                     SHCLFGCDLNPAIDRITHILMIKFLANSIEEFFQS"
     promoter        5807..5825
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5826..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5891..7801
                     /codon_start=1
                     /label=AvTnsD
                     /translation="MVSFFPQPYPDEILYSVIARYHIRSGNTSPKITLQDLFKSQTTVA
                     TVDLPSNLSNLIKNLQFISNYQVEELIYKHTLYPLYSPFLPANRSSQVLESMKGDYGGD
                     IHTRAGIMASSITMPKYLRFCPTCWEEDLKNYGEPYWHRIHQIPGILVCPFHGEVLQDS
                     LAPLHGFNKHEYYAASTDNCSITKEPSILSPDTLKKLLILAKDFWWLLNSEIPCQEPQW
                     FRKQYTNLMIEQGFATPTGRIYQNKLLDNFLFFYGQEMLSAVDSMVNHNDSNNWVTSIV
                     RKYRKSFHPLRHLLMMRFLKTSVEEFFQKDTEYKPFGEPPWLCLNAAAEHYLQPVITNL
                     VITLCCDTKKPVGTFSCACGMVYCRTGPDETDEDKVRIGKVKAFGQIWEQKLKELVEVE
                     KLGLRETARRLNVDARTIKRYVSRLKLTTYWQTRTENDSVGLQGVPEIDPNFATELKLK
                     HRQNWMALQVQNPEASKTTLRKLVPATYIWLYRNDREWLNQNSPTLQVAVPSVVRVDWH
                     ERDQQILAQVQDAVRSLLKADIPEKISISRVGKTIGLLALLEKHLDQMPLTKAYLESVV
                     ETVEEFQMRRIKWAIKLLDECGEEIMRWKVVRVAALREDCSQRVSAFLESELEKAYKKE
                     R"
     terminator      7839..7886
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8116..8931)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(8932..9023)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9041..9676)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     CDS             complement(9874..10956)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(10957..11034)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(11080..11096,1..2)
                     /label=T7 promoter
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggttaggg gtagaaggga atggactcaa gctaaatatg agcgatatat
      121 aaaagaaggt cgtggccgag gaagtggtaa gcactataaa ccttggctta caatccaaga
      181 tgttccctct aaaggacgtt cttctagaac tcctggttgg aaaacaaatc gagtacatca
      241 tttcttctca gaccatgaaa aacggctatt ttatttgttt gagtggtcag atatagtcac
      301 agatataaga gagcaatttc ctttagacaa tctcgatcta gccatgagta ttgctacaga
      361 cataggtata aagtaccctg tagatacaca aagtggcact cctcacgttt taacaagcga
      421 ctttatgctt acagtcaacc gcgatgggaa gcagatgcag attgcccgga ctgttaagcc
      481 gtcaacagaa ctggagaaaa agcaagttaa tgagaaattc gagatagaaa gacaatatta
      541 cttagcaaag ggtattgact ggggaattat cacagaaaaa gaaattccca ggcttttagc
      601 tgaaaatgtt gagtgggttc attctgctta taggctagaa gcaaattctg aaatggacac
      661 tgcacaactg agtgaattgt caacaatttt aaaatctaag cttcaggaag aagatacaac
      721 tataaataga gtaactactg ctttagacaa agaaatgaat ataaattccg gtacatcact
      781 ttatgtattt aaacatttaa ttgctaggaa agaaatcctt gtggatctag tagcaataaa
      841 attatctgct tgtccgtcaa ctaaagttat tgaaaaaata atattttaga cccacatgat
      901 aggtaaataa tatggttaaa gatttatttg tcaatgattt aatagagtgg attgtatacc
      961 aaaacactca tatagtcgaa agaattattt ggatagatga aggttacatt attgcatttg
     1021 ttatagatat taatgctaag aaaggtctac ctgaaccaag gaaaatatca gatattttag
     1081 aagctataac tgaaggtatt gcttttaagc agcaacaaga tccttgggca agaattattc
     1141 gggatgaaaa tttgacagat aaggaaaagg aatacagaga taaagcttgg gagataattt
     1201 catcattaat tatagaagaa ccatcaatat attatagaaa ttttagagga tcgatggtta
     1261 aagaagcggt tgaaaagtac aacgaagaaa gggataaaga taaattagta gaaaaaaccg
     1321 tctatggtta tttgagaaga ttttggcaga gaggaaaaac tataaatgct ctcatacctg
     1381 attttatcaa ttcatcaggt aagggcaaga ttcgaggaca atcgggcaag aagagaggaa
     1441 gaccaagaaa atatgcacat gacccagata ttggtgaagg tgtaaatgtt acagaagaag
     1501 atagaaagat ttttagagta tctataagca aattttacaa taatccaaaa gaaaactact
     1561 taacaacagc ctatgaatta atggtgaaag aatattacaa ggaaggaatt agatatgacg
     1621 agaatggtgt tcaaaaatct atattaaccc caactgataa aatacctaca attactcaat
     1681 tcaaatactg gtatcaactt gaacaggatg atgttaccaa aactttaaca tctcgaaaag
     1741 gagccaagcg agtagcttta gagaatagag caattttggg aaactctaag atggaaacta
     1801 ttggccctgg ttctagatat caaattgatg ccacagttgc tgatgtatat ttagtttcaa
     1861 aatataaccg tgactggata attggtagac cagttatcta tgtaattatt gatgttttta
     1921 gtagaatgat tacaggtgtt tatgtcggtt tagaaggccc atcttggata ggagcaatga
     1981 tggcactagc caatgtagct acagataaaa ccctgttttg ccaggaatat aacattaata
     2041 ttactgaaaa tgaatggcct tgcaatcaaa tgccagatgc aattttagga gataggggag
     2101 aattagcagg tatgacagta gaaacattaa tccctaatct ttatgttcgt attgaaaatg
     2161 cagcttccta tagagcagat tggaaagggt tagtagaaag acactttcgt ataattcatg
     2221 aatatgtaaa acctttttta cctggttatg tggatacgga ttttaggcaa agaggtgcaa
     2281 gggattatag gcttgatggc aaacttgata tagatcaatt tactgagatt attattcgtt
     2341 taattgttca ccataacaat aaacctctta ctgaatatga aagagatgaa gcaatgattg
     2401 ctgatgatac ccctcctata cctagagagt tgtggaagtg gggtatcgcc aatcgttctg
     2461 gcagactcag aactttttct gaagatattg tcaaattgaa cttgatgcct actgagaaag
     2521 caactattac tgctaaaggc atcaaaatga gaggtaagga gatgtactat acctgtgata
     2581 gtgctaaaaa tgagcaatgg tttgaaaaag ctagaagtaa tctcctttct aagtcagaaa
     2641 aatcattgat tgtatcttat gacatcagaa agccaaactt catttatctt ccatctagtg
     2701 atggtagaga ttttgaaaaa tgttttctta ttgacttaga agggcggtat gctaataaaa
     2761 actttcatga tattgaatat ctgcttgctt atgaagaatt gcaaagacag aaaagccaag
     2821 gtaaacagct acaagaaaag gtagatttaa tagctgatat tgaaagcata gtagatagag
     2881 cagaaaaaat gactgatgct tctcaatatc ataatttaag taagagcaag aaagtttctg
     2941 gaataagaga taatagggct tttgaaaagg ctaaacgacg agagaatgaa ggatttgaat
     3001 tagcaattac agataatcag agcagttttc aggttgcaga gagtacagca gaaacctccg
     3061 aacagccaga atcactacag ccaaaccatc tgaatcttct taagaaaaag cgacaggagc
     3121 gaaaacgtgg acagaattga gagtgaactg gttaaaattc ctaatggaga actagcagtt
     3181 attgccagat atatagatca aaaactgcca gaatacaaca ctaatccatt aattcaggca
     3241 ttacctccta tcctatcagc agcagaattt atagataaag tagccagaga accaaatttt
     3301 gatgaacaag agagagagct agaaccacat tatagatttc attgtattga gaggttatct
     3361 agatattttg accctcaaaa taaaactctt gaattacaaa aagttatttg tacgcttatc
     3421 atgacaggct atctgtcaag gaacctttta aaacctgaat atgcaaatcg ctctaggcaa
     3481 atttataatg caataggtaa tggaggaggt aaaaatttag aaaactatgt taatcttcct
     3541 acatctgcgt ccggcttaac tctaattggc ccatcaggta ttggaaagac tacaaatttc
     3601 aaaaatattc ttgagcttta tccccaaata atttttcatc ctgattatag tgtttaccaa
     3661 atagtttggc tgcgggtaga ttgccctaaa actttaaagg ggttatgtat agatactttt
     3721 gcaggtatcg ataaattgtt aggaactaat ttattaaaga aatttaaccc agcacgcaca
     3781 tctgaagatt atctattggc tcaacttgca caaacagcac acactcatca tctaggagtt
     3841 ttggtgattg atgaaataca aaatttagca actgcaaaaa gaaatagaga cgaaatatta
     3901 aattttttag taaagctcga taatactatt ggaattccag ttattcgtgt aggtacgaat
     3961 gaagccatta gttttcttca aggtaatctt agaaatgcta gaagaaatac tggagaagga
     4021 agtagaatgt gggatagaat ggtaaataat gatgaatggt gttacttttt agaaggaata
     4081 tgggaatacc aatggacaaa aacacctgtt catttctcgg atgaaataaa agaagttttt
     4141 tatgatgaaa gccagggaat tattgatatt gctataaaac tttacaaaat ggttcaatgg
     4201 agagcgattt caataggtga ccaagaaatt attacggttg atctgattcg tcaagtagct
     4261 aaagaaggat tgtatttagt aaagccaatg ctagatgcaa ttaggttagg ggacagagag
     4321 tggatgatta aatataagga tattgctcct ctcgatatta cggaatatca caatagatgt
     4381 gtatctgaga ttgatgctaa agatttgaca gagattaaga gattagctaa aaagcagcag
     4441 aatcagcaga aagtatctcc gaagctcaat tatgtaatta gtgagctgtt gaaattggaa
     4501 gtagaatctg gtaaagcaaa ggaatgtgca gaacaaattg tttatagtca tgaagatcca
     4561 gacattccaa ttccatcttt agttaataag gcatatgctc ttgctttgca aggatgtcaa
     4621 gtaaatgaga gtcttgctaa aacaagtacc aggaaagcta aattaaagac aaagtaccaa
     4681 gagaatgata ttcggcgaat tgtcgagaat gccaagaaag acaaaaaatc tgcatacgag
     4741 gatttgaaat tagcaggtat aatcaaaaat ccagttgaag attttatcaa tatttagctt
     4801 ctaaatgctt agttttttcc caactctgta tcccgatgaa cttttatata gcactttagc
     4861 tagataccat attcggagtg gaaataaaag tttcaagcaa actgacttag agctattcgg
     4921 ttacagttcc caacaaatat gcaaaattac cttaactaat aatttaaatt atttagtgaa
     4981 aaacttacct ttacgctccc agcaaaaagt tgagaattta ctacaaaatc atactttata
     5041 ttccttttat gcaactttat taatgccgca ggaggcttgg ctgctgaaaa actcaatgag
     5101 taataaattc agtgggtcta tactagattt tgctaaagtg gctaataatt ctagtgatga
     5161 ttcaaaaaaa tttttaaaat tttgccctga atgtttagag aaagataccc aaaagtatgg
     5221 tgaaccttac tggcatagaa tacatcaagt ccctggcatt ttagtttgtc caattcatcg
     5281 tgttgtttta cacaatagct gtatagaagt tgaatcgaaa ggcatacatt atcatgctgc
     5341 cagcatagat aattgtttat tagcaaataa tgtgaaaaaa ttgacaaata aaactgtaga
     5401 aacactttca atactggcac atgatattaa ttggctaatt catagcagtt ttgcctttca
     5461 aggtttggct tggcttcgcg ctcagtacca gcgttactta ttggataaag gactgctaaa
     5521 aacctttgct ggtggcaagt ttaagtttaa tgattgtggt tttgcccagc ttatatttga
     5581 gttttatggt caagaatcct tagaagccct caatccaaag ctgacccaaa atcaaggtaa
     5641 atatttttct cactgcttgt ttggatgcga cttaaaccca gcaattgacc gcataactca
     5701 tatcttaatg ataaaatttt tagcaaattc tatagaagag ttctttcagt cataacttaa
     5761 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
     5821 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
     5881 ggagatatac atggtgagtt ttttccctca accttatcca gatgaaattt tatatagtgt
     5941 tattgctcgt tatcacatta gaagtggcaa tacgagtcca aaaattacat tacaagattt
     6001 atttaagtca cagactactg tggcgacagt agatttacca tcgaacctta gtaatttaat
     6061 taaaaaccta caatttatct caaattacca agttgaagag ttgatatata aacatactct
     6121 ctacccttta tatagccctt ttttgccagc aaacaggtct agtcaggtac tggaatctat
     6181 gaaaggagat tatgggggag atattcatac aagagccgga attatggcaa gctcaattac
     6241 tatgccgaaa tatttgcggt tttgtccaac ttgctgggaa gaagacttaa aaaattatgg
     6301 cgaaccttac tggcatcgca tccatcaaat accagggatt ttagtttgtc catttcatgg
     6361 tgaagttttg caagatagcc tagctccttt acatggtttt aataagcatg aatattatgc
     6421 agctagtaca gacaactgtt ctattactaa agaacccagt attcttagcc ctgacacact
     6481 gaaaaaatta ctaatattag ccaaagattt ttggtggtta ctcaatagtg aaataccttg
     6541 ccaagagcca cagtggtttc gcaaacagta cacaaatctg atgattgagc agggttttgc
     6601 aactccaact ggtcgaattt accagaacaa actactagat aattttttgt tcttttatgg
     6661 tcaagaaatg ctcagtgctg ttgattcaat ggtaaatcat aatgattcaa acaactgggt
     6721 tactagcatc gtcaggaaat ataggaaatc tttccatcct cttagacatc tgctaatgat
     6781 gcgttttttg aaaacatcag tagaggaatt ttttcaaaaa gatactgaat acaagccttt
     6841 tggcgaaccc ccctggttgt gtctcaatgc tgccgcagaa cattatctac aaccagtgat
     6901 taccaattta gtcattactt tatgctgcga tacaaaaaag cctgtcggta ctttttcttg
     6961 tgcttgcgga atggtttatt gccggactgg gcctgatgaa acggatgaag acaaagttcg
     7021 cattggtaaa gtcaaggcat tcggtcaaat atgggagcaa aagctgaaag aattggtgga
     7081 agttgaaaag ttgggtttgc gggagacagc cagaagatta aatgttgatg ccagaactat
     7141 caaacgttat gtatctcgtc taaaattgac aacttattgg cagactagaa cagaaaatga
     7201 ctctgtaggc ttacagggag tcccagaaat agatcccaat tttgcaactg aactcaaatt
     7261 aaaacacagg caaaattgga tggctttaca agtacagaac cctgaagctt caaagacaac
     7321 tctgagaaaa ctagtaccag ccacctatat ttggctatac aggaatgaca gagaatggct
     7381 caatcaaaac tcacccactt tacaagttgc cgttccttcc gttgtgagag ttgattggca
     7441 tgagcgagat cagcaaattc tcgctcaggt tcaagatgca gtgcgatcgc tacttaaggc
     7501 tgatatacca gagaaaattt ctattagtag ggtagggaaa actatcggct tacttgcatt
     7561 actcgaaaaa catctcgacc agatgccttt aacaaaggct tatttggaat cagtcgttga
     7621 aactgtagaa gaatttcaga tgcgccggat caagtgggca atcaagctgc ttgatgaatg
     7681 tggagaagaa attatgcgct ggaaggtagt cagagttgct gctttgcgag aggattgctc
     7741 ccaaagagtc agtgcatttc tggaaagtga actggagaaa gcatacaaga aagagaggtg
     7801 attaattaac ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     7861 ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca
     7921 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     7981 gaccctgccc tgaaccgacg acaagctgac gaccgggtct ccgcaagtgg cacttttcgg
     8041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8101 ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     8161 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     8221 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     8281 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     8341 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     8401 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     8461 attcattcgt gattgcgcct gagcgagacg aaatacgcgg tcgctgttaa aaggacaatt
     8521 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     8581 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     8641 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     8701 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     8761 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     8821 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     8881 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca tactcttcct
     8941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9001 atgtatttag aaaaataaac aaataggcat gctagcgcag aaacgtccta gaagatgcca
     9061 ggaggatact tagcagagag acaataaggc cggagcgaag ccgtttttcc ataggctccg
     9121 cccccctgac gaacatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     9181 actataaaga taccaggcgt ttccccctga tggctccctc ttgcgctctc ctgttcccgt
     9241 cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc accacgtccc gttccgtgta
     9301 gacagttcgc tccaagctgg gctgtgtgca agaacccccc gttcagcccg actgctgcgc
     9361 cttatccggt aactatcatc ttgagtccaa cccggaaaga cacgacaaaa cgccactggc
     9421 agcagccatt ggtaactgag aattagtgga tttagatatc gagagtcttg aagtggtggc
     9481 ctaacagagg ctacactgaa aggacagtat ttggtatctg cgctccacta aagccagtta
     9541 ccaggttaag cagttcccca actgacttaa ccttcgatca aaccgcctcc ccaggcggtt
     9601 ttttcgttta cagagcagga gattacgacg atcgtaaaag gatctcaaga agatccttta
     9661 cggattcccg acaccatcac tctagatttc agtgcaattt atctcttcaa atgtagcacc
     9721 tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgcc ccgcgcccac
     9781 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     9841 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9961 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
    10021 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
    10081 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
    10141 tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
    10201 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
    10261 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
    10321 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
    10381 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
    10441 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
    10501 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
    10561 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
    10621 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
    10681 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
    10741 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
    10801 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
    10861 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
    10921 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
    10981 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
    11041 ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc actata
//
