LOCUS       Exported                9185 bp ds-DNA     circular SYN 06-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             71..889
                     /codon_start=1
                     /label=AvTnsA
                     /translation="MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSS
                     RTPGWKTNRVHHFFSDHEKRLFYLFEWSDIVTDIREQFPLDNLDLAMSIATDIGIKYPV
                     DTQSGTPHVLTSDFMLTVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAKGID
                     WGIITEKEIPRLLAENVEWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVT
                     TALDKEMNINSGTSLYVFKHLIARKEILVDLVAIKLSACPSTKVIEKIIF"
     CDS             912..3140
                     /codon_start=1
                     /label=AvTnsB
                     /translation="MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKG
                     LPEPRKISDILEAITEGIAFKQQQDPWARIIRDENLTDKEKEYRDKAWEIISSLIIEEP
                     SIYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFINSSG
                     KGKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYE
                     LMVKEYYKEGIRYDENGVQKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRV
                     ALENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNRDWIIGRPVIYVIIDVFSRMI
                     TGVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELA
                     GMTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARD
                     YRLDGKLDIDQFTEIIIRLIVHHNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSG
                     RLRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKNEQWFEKARSNLLSKSE
                     KSLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKS
                     QGKQLQEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEG
                     FELAITDNQSSFQVAESTAETSEQPESLQPNHLNLLKKKRQERKRGQN"
     CDS             3127..4797
                     /codon_start=1
                     /label=AvTnsC
                     /translation="VDRIESELVKIPNGELAVIARYIDQKLPEYNTNPLIQALPPILSA
                     AEFIDKVAREPNFDEQERELEPHYRFHCIERLSRYFDPQNKTLELQKVICTLIMTGYLS
                     RNLLKPEYANRSRQIYNAIGNGGGKNLENYVNLPTSASGLTLIGPSGIGKTTNFKNILE
                     LYPQIIFHPDYSVYQIVWLRVDCPKTLKGLCIDTFAGIDKLLGTNLLKKFNPARTSEDY
                     LLAQLAQTAHTHHLGVLVIDEIQNLATAKRNRDEILNFLVKLDNTIGIPVIRVGTNEAI
                     SFLQGNLRNARRNTGEGSRMWDRMVNNDEWCYFLEGIWEYQWTKTPVHFSDEIKEVFYD
                     ESQGIIDIAIKLYKMVQWRAISIGDQEIITVDLIRQVAKEGLYLVKPMLDAIRLGDREW
                     MIKYKDIAPLDITEYHNRCVSEIDAKDLTEIKRLAKKQQNQQKVSPKLNYVISELLKLE
                     VESGKAKECAEQIVYSHEDPDIPIPSLVNKAYALALQGCQVNESLAKTSTRKAKLKTKY
                     QENDIRRIVENAKKDKKSAYEDLKLAGIIKNPVEDFINI"
     CDS             4805..5755
                     /codon_start=1
                     /label=AvTniQ
                     /translation="MLSFFPTLYPDELLYSTLARYHIRSGNKSFKQTDLELFGYSSQQI
                     CKITLTNNLNYLVKNLPLRSQQKVENLLQNHTLYSFYATLLMPQEAWLLKNSMSNKFSG
                     SILDFAKVANNSSDDSKKFLKFCPECLEKDTQKYGEPYWHRIHQVPGILVCPIHRVVLH
                     NSCIEVESKGIHYHAASIDNCLLANNVKKLTNKTVETLSILAHDINWLIHSSFAFQGLA
                     WLRAQYQRYLLDKGLLKTFAGGKFKFNDCGFAQLIFEFYGQESLEALNPKLTQNQGKYF
                     SHCLFGCDLNPAIDRITHILMIKFLANSIEEFFQS"
     promoter        5807..5825
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5826..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5928..5975
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6205..7020)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(7021..7112)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7130..7765)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     CDS             complement(7963..9045)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(9046..9123)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(9169..9185,1..2)
                     /label=T7 promoter
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggttaggg gtagaaggga atggactcaa gctaaatatg agcgatatat
      121 aaaagaaggt cgtggccgag gaagtggtaa gcactataaa ccttggctta caatccaaga
      181 tgttccctct aaaggacgtt cttctagaac tcctggttgg aaaacaaatc gagtacatca
      241 tttcttctca gaccatgaaa aacggctatt ttatttgttt gagtggtcag atatagtcac
      301 agatataaga gagcaatttc ctttagacaa tctcgatcta gccatgagta ttgctacaga
      361 cataggtata aagtaccctg tagatacaca aagtggcact cctcacgttt taacaagcga
      421 ctttatgctt acagtcaacc gcgatgggaa gcagatgcag attgcccgga ctgttaagcc
      481 gtcaacagaa ctggagaaaa agcaagttaa tgagaaattc gagatagaaa gacaatatta
      541 cttagcaaag ggtattgact ggggaattat cacagaaaaa gaaattccca ggcttttagc
      601 tgaaaatgtt gagtgggttc attctgctta taggctagaa gcaaattctg aaatggacac
      661 tgcacaactg agtgaattgt caacaatttt aaaatctaag cttcaggaag aagatacaac
      721 tataaataga gtaactactg ctttagacaa agaaatgaat ataaattccg gtacatcact
      781 ttatgtattt aaacatttaa ttgctaggaa agaaatcctt gtggatctag tagcaataaa
      841 attatctgct tgtccgtcaa ctaaagttat tgaaaaaata atattttaga cccacatgat
      901 aggtaaataa tatggttaaa gatttatttg tcaatgattt aatagagtgg attgtatacc
      961 aaaacactca tatagtcgaa agaattattt ggatagatga aggttacatt attgcatttg
     1021 ttatagatat taatgctaag aaaggtctac ctgaaccaag gaaaatatca gatattttag
     1081 aagctataac tgaaggtatt gcttttaagc agcaacaaga tccttgggca agaattattc
     1141 gggatgaaaa tttgacagat aaggaaaagg aatacagaga taaagcttgg gagataattt
     1201 catcattaat tatagaagaa ccatcaatat attatagaaa ttttagagga tcgatggtta
     1261 aagaagcggt tgaaaagtac aacgaagaaa gggataaaga taaattagta gaaaaaaccg
     1321 tctatggtta tttgagaaga ttttggcaga gaggaaaaac tataaatgct ctcatacctg
     1381 attttatcaa ttcatcaggt aagggcaaga ttcgaggaca atcgggcaag aagagaggaa
     1441 gaccaagaaa atatgcacat gacccagata ttggtgaagg tgtaaatgtt acagaagaag
     1501 atagaaagat ttttagagta tctataagca aattttacaa taatccaaaa gaaaactact
     1561 taacaacagc ctatgaatta atggtgaaag aatattacaa ggaaggaatt agatatgacg
     1621 agaatggtgt tcaaaaatct atattaaccc caactgataa aatacctaca attactcaat
     1681 tcaaatactg gtatcaactt gaacaggatg atgttaccaa aactttaaca tctcgaaaag
     1741 gagccaagcg agtagcttta gagaatagag caattttggg aaactctaag atggaaacta
     1801 ttggccctgg ttctagatat caaattgatg ccacagttgc tgatgtatat ttagtttcaa
     1861 aatataaccg tgactggata attggtagac cagttatcta tgtaattatt gatgttttta
     1921 gtagaatgat tacaggtgtt tatgtcggtt tagaaggccc atcttggata ggagcaatga
     1981 tggcactagc caatgtagct acagataaaa ccctgttttg ccaggaatat aacattaata
     2041 ttactgaaaa tgaatggcct tgcaatcaaa tgccagatgc aattttagga gataggggag
     2101 aattagcagg tatgacagta gaaacattaa tccctaatct ttatgttcgt attgaaaatg
     2161 cagcttccta tagagcagat tggaaagggt tagtagaaag acactttcgt ataattcatg
     2221 aatatgtaaa acctttttta cctggttatg tggatacgga ttttaggcaa agaggtgcaa
     2281 gggattatag gcttgatggc aaacttgata tagatcaatt tactgagatt attattcgtt
     2341 taattgttca ccataacaat aaacctctta ctgaatatga aagagatgaa gcaatgattg
     2401 ctgatgatac ccctcctata cctagagagt tgtggaagtg gggtatcgcc aatcgttctg
     2461 gcagactcag aactttttct gaagatattg tcaaattgaa cttgatgcct actgagaaag
     2521 caactattac tgctaaaggc atcaaaatga gaggtaagga gatgtactat acctgtgata
     2581 gtgctaaaaa tgagcaatgg tttgaaaaag ctagaagtaa tctcctttct aagtcagaaa
     2641 aatcattgat tgtatcttat gacatcagaa agccaaactt catttatctt ccatctagtg
     2701 atggtagaga ttttgaaaaa tgttttctta ttgacttaga agggcggtat gctaataaaa
     2761 actttcatga tattgaatat ctgcttgctt atgaagaatt gcaaagacag aaaagccaag
     2821 gtaaacagct acaagaaaag gtagatttaa tagctgatat tgaaagcata gtagatagag
     2881 cagaaaaaat gactgatgct tctcaatatc ataatttaag taagagcaag aaagtttctg
     2941 gaataagaga taatagggct tttgaaaagg ctaaacgacg agagaatgaa ggatttgaat
     3001 tagcaattac agataatcag agcagttttc aggttgcaga gagtacagca gaaacctccg
     3061 aacagccaga atcactacag ccaaaccatc tgaatcttct taagaaaaag cgacaggagc
     3121 gaaaacgtgg acagaattga gagtgaactg gttaaaattc ctaatggaga actagcagtt
     3181 attgccagat atatagatca aaaactgcca gaatacaaca ctaatccatt aattcaggca
     3241 ttacctccta tcctatcagc agcagaattt atagataaag tagccagaga accaaatttt
     3301 gatgaacaag agagagagct agaaccacat tatagatttc attgtattga gaggttatct
     3361 agatattttg accctcaaaa taaaactctt gaattacaaa aagttatttg tacgcttatc
     3421 atgacaggct atctgtcaag gaacctttta aaacctgaat atgcaaatcg ctctaggcaa
     3481 atttataatg caataggtaa tggaggaggt aaaaatttag aaaactatgt taatcttcct
     3541 acatctgcgt ccggcttaac tctaattggc ccatcaggta ttggaaagac tacaaatttc
     3601 aaaaatattc ttgagcttta tccccaaata atttttcatc ctgattatag tgtttaccaa
     3661 atagtttggc tgcgggtaga ttgccctaaa actttaaagg ggttatgtat agatactttt
     3721 gcaggtatcg ataaattgtt aggaactaat ttattaaaga aatttaaccc agcacgcaca
     3781 tctgaagatt atctattggc tcaacttgca caaacagcac acactcatca tctaggagtt
     3841 ttggtgattg atgaaataca aaatttagca actgcaaaaa gaaatagaga cgaaatatta
     3901 aattttttag taaagctcga taatactatt ggaattccag ttattcgtgt aggtacgaat
     3961 gaagccatta gttttcttca aggtaatctt agaaatgcta gaagaaatac tggagaagga
     4021 agtagaatgt gggatagaat ggtaaataat gatgaatggt gttacttttt agaaggaata
     4081 tgggaatacc aatggacaaa aacacctgtt catttctcgg atgaaataaa agaagttttt
     4141 tatgatgaaa gccagggaat tattgatatt gctataaaac tttacaaaat ggttcaatgg
     4201 agagcgattt caataggtga ccaagaaatt attacggttg atctgattcg tcaagtagct
     4261 aaagaaggat tgtatttagt aaagccaatg ctagatgcaa ttaggttagg ggacagagag
     4321 tggatgatta aatataagga tattgctcct ctcgatatta cggaatatca caatagatgt
     4381 gtatctgaga ttgatgctaa agatttgaca gagattaaga gattagctaa aaagcagcag
     4441 aatcagcaga aagtatctcc gaagctcaat tatgtaatta gtgagctgtt gaaattggaa
     4501 gtagaatctg gtaaagcaaa ggaatgtgca gaacaaattg tttatagtca tgaagatcca
     4561 gacattccaa ttccatcttt agttaataag gcatatgctc ttgctttgca aggatgtcaa
     4621 gtaaatgaga gtcttgctaa aacaagtacc aggaaagcta aattaaagac aaagtaccaa
     4681 gagaatgata ttcggcgaat tgtcgagaat gccaagaaag acaaaaaatc tgcatacgag
     4741 gatttgaaat tagcaggtat aatcaaaaat ccagttgaag attttatcaa tatttagctt
     4801 ctaaatgctt agttttttcc caactctgta tcccgatgaa cttttatata gcactttagc
     4861 tagataccat attcggagtg gaaataaaag tttcaagcaa actgacttag agctattcgg
     4921 ttacagttcc caacaaatat gcaaaattac cttaactaat aatttaaatt atttagtgaa
     4981 aaacttacct ttacgctccc agcaaaaagt tgagaattta ctacaaaatc atactttata
     5041 ttccttttat gcaactttat taatgccgca ggaggcttgg ctgctgaaaa actcaatgag
     5101 taataaattc agtgggtcta tactagattt tgctaaagtg gctaataatt ctagtgatga
     5161 ttcaaaaaaa tttttaaaat tttgccctga atgtttagag aaagataccc aaaagtatgg
     5221 tgaaccttac tggcatagaa tacatcaagt ccctggcatt ttagtttgtc caattcatcg
     5281 tgttgtttta cacaatagct gtatagaagt tgaatcgaaa ggcatacatt atcatgctgc
     5341 cagcatagat aattgtttat tagcaaataa tgtgaaaaaa ttgacaaata aaactgtaga
     5401 aacactttca atactggcac atgatattaa ttggctaatt catagcagtt ttgcctttca
     5461 aggtttggct tggcttcgcg ctcagtacca gcgttactta ttggataaag gactgctaaa
     5521 aacctttgct ggtggcaagt ttaagtttaa tgattgtggt tttgcccagc ttatatttga
     5581 gttttatggt caagaatcct tagaagccct caatccaaag ctgacccaaa atcaaggtaa
     5641 atatttttct cactgcttgt ttggatgcga cttaaaccca gcaattgacc gcataactca
     5701 tatcttaatg ataaaatttt tagcaaattc tatagaagag ttctttcagt cataacttaa
     5761 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
     5821 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
     5881 ggagatatac ttaattaacc taggctgctg ccaccgctga gcaataacta gcataacccc
     5941 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc
     6001 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     6061 ctatttaacg accctgccct gaaccgacga caagctgacg accgggtctc cgcaagtggc
     6121 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6181 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
     6241 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     6301 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     6361 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     6421 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     6481 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     6541 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcggt cgctgttaaa
     6601 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     6661 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     6721 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     6781 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     6841 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     6901 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     6961 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     7021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7081 catatttgaa tgtatttaga aaaataaaca aataggcatg ctagcgcaga aacgtcctag
     7141 aagatgccag gaggatactt agcagagaga caataaggcc ggagcgaagc cgtttttcca
     7201 taggctccgc ccccctgacg aacatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     7261 cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctct tgcgctctcc
     7321 tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt acccaaatca ccacgtcccg
     7381 ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa gaaccccccg ttcagcccga
     7441 ctgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac acgacaaaac
     7501 gccactggca gcagccattg gtaactgaga attagtggat ttagatatcg agagtcttga
     7561 agtggtggcc taacagaggc tacactgaaa ggacagtatt tggtatctgc gctccactaa
     7621 agccagttac caggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc
     7681 caggcggttt tttcgtttac agagcaggag attacgacga tcgtaaaagg atctcaagaa
     7741 gatcctttac ggattcccga caccatcact ctagatttca gtgcaattta tctcttcaaa
     7801 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc
     7861 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     7921 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     7981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8041 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     8101 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     8161 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     8221 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     8281 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     8341 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     8401 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     8461 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     8521 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     8581 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     8641 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     8701 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     8761 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     8821 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     8881 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     8941 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     9001 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     9061 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     9121 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta atacgactca
     9181 ctata
//
