LOCUS       Exported                3572 bp ds-DNA     circular UNA 06-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...1q-cyc16jn
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: 3f2e35cc-83ae-4267-861b-ee70dd43380e 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    5..156
                     /label=AvCAST_RE
     misc_feature    157..630
                     /label=Cargo
     misc_feature    631..925
                     /label=AvCAST_LE
     primer_bind     complement(943..959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1433..1537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1538..2398
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2569..3157
                     /direction=RIGHT
                     /label=pUC ori
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3445..3466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3481..3511
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3519..3535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3543..3559
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgtgga ggaataattc ggttcaacaa tatataattc ggttcaacaa ttaataaaac
       61 gattgaacaa taacctctaa aggagtacga ttgtaccaag taaaagtgcg atcgcttcca
      121 gaggttattt ttatggttag gggtagaagg gaatggcggg ttcgtgagtg gcttccctgc
      181 cgcggccgcc gcgggcgcgg gccgctcgct ctacggtggg cccgagctcg tgttccccga
      241 ggccatgaac caccccgcgc tgaccgtgca tccggcgcac cagctgggcg cctccccgct
      301 gcagcccccg cactccttct tcggcgccca gcaccgggac cctctccatt tctacccctg
      361 ggtcctgcgg aaccgcttct tcggccaccg cttccagggt gagtgtccac gctgtgcccg
      421 ccgaggcggc cggccggcgc ccgtgctgcg gcgatgcggg ggaggctcgg gggcgcgcgg
      481 ggctgtttag aagttactgc cgggaaggct gcaggtccgc ggaggtagat tcccaggcag
      541 ggaagagctg tgcggcatcc acccgcgcct tcgccgcgta ggtctccctc ccaggaaagc
      601 aggtggagat attggtgccc ttctagagtc ccgggatatt tacgttttgt aatcacgaat
      661 agattcttga ttgcaagatg aattgcgatc gcatctttgt cgcacagcag aactttctca
      721 aataattttg aacttatgga attactcaaa acctttatca gtaatggatt caaacaattg
      781 tttaaccgtt ttatttgaga tttttactaa aaatcaagtt taccatagag gttaagctga
      841 aattgttcaa tcgttttatt ttatcgacaa cggcagatta aatattcgaa ctgtaataat
      901 tgtttaaccg aattctttgt ctacagtacc gagctcgaat tcactggccg tcgttttaca
      961 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1081 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1141 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1201 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1261 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1321 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1381 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1441 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1501 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1561 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1621 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1681 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1741 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1801 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1861 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1921 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1981 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2041 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2101 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2161 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2221 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2281 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2341 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2701 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3181 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3301 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3361 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3421 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     3481 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     3541 cacaggaaac agctatgacc atgattacgc ca
//
