LOCUS       Exported                8309 bp ds-DNA     circular UNA 07-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...6l-cyfg2ai
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: da58e375-cec9-414c-af14-f7f53b755a41 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1020..1211
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1313..1452
                     /label=bom
     rep_origin      complement(1620..2302)
                     /direction=LEFT
                     /label=ColE1 ori
                     /label=ColE1 origin
     CDS             complement(2397..3059)
                     /label=AmpR
     promoter        3775..3793
                     /label=T7 promoter
     misc_feature    3794..3818
                     /label=lac operator
     misc_feature    3865..4014
                     /label=6x His tag/ Twin Strep
     misc_feature    4015..4308
                     /label=Sumo
     CDS             4312..6537
                     /codon_start=1
                     /label=AvTnsB
                     /label=TnsB
                     /translation="VKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGL
                     PEPRKISDILEAITEGIAFKQQQDPWARIIRDENLTDKEKEYRDKAWEIISSLIIEEPS
                     IYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFINSSGK
                     GKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYEL
                     MVKEYYKEGIRYDENGVQKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVA
                     LENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNRDWIIGRPVIYVIIDVFSRMIT
                     GVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAG
                     MTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDY
                     RLDGKLDIDQFTEIIIRLIVHHNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGR
                     LRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKNEQWFEKARSNLLSKSEK
                     SLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQ
                     GKQLQEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGF
                     ELAITDNQSSFQVAESTAETSEQPESLQPNHLNLLKKKRQERKRGQN"
     terminator      6598..6645
                     /label=T7 terminator
     promoter        6858..6934
                     /label=lacI promoter
     CDS             6935..8017
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaaccggg atctcgacgc
     3481 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3541 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     3601 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3661 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3721 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
     3781 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3841 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
     3901 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
     3961 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
     4021 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
     4081 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
     4141 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
     4201 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
     4261 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc cgttaaagat
     4321 ttatttgtca atgatttaat agagtggatt gtataccaaa acactcatat agtcgaaaga
     4381 attatttgga tagatgaagg ttacattatt gcatttgtta tagatattaa tgctaagaaa
     4441 ggtctacctg aaccaaggaa aatatcagat attttagaag ctataactga aggtattgct
     4501 tttaagcagc aacaagatcc ttgggcaaga attattcggg atgaaaattt gacagataag
     4561 gaaaaggaat acagagataa agcttgggag ataatttcat cattaattat agaagaacca
     4621 tcaatatatt atagaaattt tagaggatcg atggttaaag aagcggttga aaagtacaac
     4681 gaagaaaggg ataaagataa attagtagaa aaaaccgtct atggttattt gagaagattt
     4741 tggcagagag gaaaaactat aaatgctctc atacctgatt ttatcaattc atcaggtaag
     4801 ggcaagattc gaggacaatc gggcaagaag agaggaagac caagaaaata tgcacatgac
     4861 ccagatattg gtgaaggtgt aaatgttaca gaagaagata gaaagatttt tagagtatct
     4921 ataagcaaat tttacaataa tccaaaagaa aactacttaa caacagccta tgaattaatg
     4981 gtgaaagaat attacaagga aggaattaga tatgacgaga atggtgttca aaaatctata
     5041 ttaaccccaa ctgataaaat acctacaatt actcaattca aatactggta tcaacttgaa
     5101 caggatgatg ttaccaaaac tttaacatct cgaaaaggag ccaagcgagt agctttagag
     5161 aatagagcaa ttttgggaaa ctctaagatg gaaactattg gccctggttc tagatatcaa
     5221 attgatgcca cagttgctga tgtatattta gtttcaaaat ataaccgtga ctggataatt
     5281 ggtagaccag ttatctatgt aattattgat gtttttagta gaatgattac aggtgtttat
     5341 gtcggtttag aaggcccatc ttggatagga gcaatgatgg cactagccaa tgtagctaca
     5401 gataaaaccc tgttttgcca ggaatataac attaatatta ctgaaaatga atggccttgc
     5461 aatcaaatgc cagatgcaat tttaggagat aggggagaat tagcaggtat gacagtagaa
     5521 acattaatcc ctaatcttta tgttcgtatt gaaaatgcag cttcctatag agcagattgg
     5581 aaagggttag tagaaagaca ctttcgtata attcatgaat atgtaaaacc ttttttacct
     5641 ggttatgtgg atacggattt taggcaaaga ggtgcaaggg attataggct tgatggcaaa
     5701 cttgatatag atcaatttac tgagattatt attcgtttaa ttgttcacca taacaataaa
     5761 cctcttactg aatatgaaag agatgaagca atgattgctg atgatacccc tcctatacct
     5821 agagagttgt ggaagtgggg tatcgccaat cgttctggca gactcagaac tttttctgaa
     5881 gatattgtca aattgaactt gatgcctact gagaaagcaa ctattactgc taaaggcatc
     5941 aaaatgagag gtaaggagat gtactatacc tgtgatagtg ctaaaaatga gcaatggttt
     6001 gaaaaagcta gaagtaatct cctttctaag tcagaaaaat cattgattgt atcttatgac
     6061 atcagaaagc caaacttcat ttatcttcca tctagtgatg gtagagattt tgaaaaatgt
     6121 tttcttattg acttagaagg gcggtatgct aataaaaact ttcatgatat tgaatatctg
     6181 cttgcttatg aagaattgca aagacagaaa agccaaggta aacagctaca agaaaaggta
     6241 gatttaatag ctgatattga aagcatagta gatagagcag aaaaaatgac tgatgcttct
     6301 caatatcata atttaagtaa gagcaagaaa gtttctggaa taagagataa tagggctttt
     6361 gaaaaggcta aacgacgaga gaatgaagga tttgaattag caattacaga taatcagagc
     6421 agttttcagg ttgcagagag tacagcagaa acctccgaac agccagaatc actacagcca
     6481 aaccatctga atcttcttaa gaaaaagcga caggagcgaa aacgtggaca gaattgagcg
     6541 gccgcactcg aggcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     6601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     6661 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     6721 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     6781 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
     6841 gctgtcaaac atgagaaaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
     6901 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
     6961 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     7021 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     7081 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     7141 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     7201 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     7261 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     7321 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     7381 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     7441 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     7501 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     7561 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     7621 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     7681 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     7741 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     7801 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     7861 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     7921 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7981 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
     8041 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
     8101 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     8161 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     8221 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     8281 tcgctcaagc cttcgtcact ggtcccgcc
//
