LOCUS       Exported                7991 bp ds-DNA     circular UNA 07-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...6l-cyfg2ai
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: da58e375-cec9-414c-af14-f7f53b755a41 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1020..1211
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1313..1452
                     /label=bom
     rep_origin      complement(1620..2302)
                     /direction=LEFT
                     /label=ColE1 ori
                     /label=ColE1 origin
     CDS             complement(2397..3059)
                     /label=AmpR
     promoter        3775..3793
                     /label=T7 promoter
     misc_feature    3794..3818
                     /label=lac operator
     misc_feature    3865..4014
                     /label=6x His tag/ Twin Strep
     misc_feature    4015..4308
                     /label=Sumo
     CDS             4312..6219
                     /codon_start=1
                     /label=AvTnsD
                     /label=TniQ2
                     /translation="VSFFPQPYPDEILYSVIARYHIRSGNTSPKITLQDLFKSQTTVAT
                     VDLPSNLSNLIKNLQFISNYQVEELIYKHTLYPLYSPFLPANRSSQVLESMKGDYGGDI
                     HTRAGIMASSITMPKYLRFCPTCWEEDLKNYGEPYWHRIHQIPGILVCPFHGEVLQDSL
                     APLHGFNKHEYYAASTDNCSITKEPSILSPDTLKKLLILAKDFWWLLNSEIPCQEPQWF
                     RKQYTNLMIEQGFATPTGRIYQNKLLDNFLFFYGQEMLSAVDSMVNHNDSNNWVTSIVR
                     KYRKSFHPLRHLLMMRFLKTSVEEFFQKDTEYKPFGEPPWLCLNAAAEHYLQPVITNLV
                     ITLCCDTKKPVGTFSCACGMVYCRTGPDETDEDKVRIGKVKAFGQIWEQKLKELVEVEK
                     LGLRETARRLNVDARTIKRYVSRLKLTTYWQTRTENDSVGLQGVPEIDPNFATELKLKH
                     RQNWMALQVQNPEASKTTLRKLVPATYIWLYRNDREWLNQNSPTLQVAVPSVVRVDWHE
                     RDQQILAQVQDAVRSLLKADIPEKISISRVGKTIGLLALLEKHLDQMPLTKAYLESVVE
                     TVEEFQMRRIKWAIKLLDECGEEIMRWKVVRVAALREDCSQRVSAFLESELEKAYKKER
                     "
     terminator      6280..6327
                     /label=T7 terminator
     promoter        6540..6616
                     /label=lacI promoter
     CDS             6617..7699
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaaccggg atctcgacgc
     3481 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3541 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     3601 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3661 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3721 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
     3781 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3841 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
     3901 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
     3961 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
     4021 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
     4081 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
     4141 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
     4201 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
     4261 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc cgtgagtttt
     4321 ttccctcaac cttatccaga tgaaatttta tatagtgtta ttgctcgtta tcacattaga
     4381 agtggcaata cgagtccaaa aattacatta caagatttat ttaagtcaca gactactgtg
     4441 gcgacagtag atttaccatc gaaccttagt aatttaatta aaaacctaca atttatctca
     4501 aattaccaag ttgaagagtt gatatataaa catactctct accctttata tagccctttt
     4561 ttgccagcaa acaggtctag tcaggtactg gaatctatga aaggagatta tgggggagat
     4621 attcatacaa gagccggaat tatggcaagc tcaattacta tgccgaaata tttgcggttt
     4681 tgtccaactt gctgggaaga agacttaaaa aattatggcg aaccttactg gcatcgcatc
     4741 catcaaatac cagggatttt agtttgtcca tttcatggtg aagttttgca agatagccta
     4801 gctcctttac atggttttaa taagcatgaa tattatgcag ctagtacaga caactgttct
     4861 attactaaag aacccagtat tcttagccct gacacactga aaaaattact aatattagcc
     4921 aaagattttt ggtggttact caatagtgaa ataccttgcc aagagccaca gtggtttcgc
     4981 aaacagtaca caaatctgat gattgagcag ggttttgcaa ctccaactgg tcgaatttac
     5041 cagaacaaac tactagataa ttttttgttc ttttatggtc aagaaatgct cagtgctgtt
     5101 gattcaatgg taaatcataa tgattcaaac aactgggtta ctagcatcgt caggaaatat
     5161 aggaaatctt tccatcctct tagacatctg ctaatgatgc gttttttgaa aacatcagta
     5221 gaggaatttt ttcaaaaaga tactgaatac aagccttttg gcgaaccccc ctggttgtgt
     5281 ctcaatgctg ccgcagaaca ttatctacaa ccagtgatta ccaatttagt cattacttta
     5341 tgctgcgata caaaaaagcc tgtcggtact ttttcttgtg cttgcggaat ggtttattgc
     5401 cggactgggc ctgatgaaac ggatgaagac aaagttcgca ttggtaaagt caaggcattc
     5461 ggtcaaatat gggagcaaaa gctgaaagaa ttggtggaag ttgaaaagtt gggtttgcgg
     5521 gagacagcca gaagattaaa tgttgatgcc agaactatca aacgttatgt atctcgtcta
     5581 aaattgacaa cttattggca gactagaaca gaaaatgact ctgtaggctt acagggagtc
     5641 ccagaaatag atcccaattt tgcaactgaa ctcaaattaa aacacaggca aaattggatg
     5701 gctttacaag tacagaaccc tgaagcttca aagacaactc tgagaaaact agtaccagcc
     5761 acctatattt ggctatacag gaatgacaga gaatggctca atcaaaactc acccacttta
     5821 caagttgccg ttccttccgt tgtgagagtt gattggcatg agcgagatca gcaaattctc
     5881 gctcaggttc aagatgcagt gcgatcgcta cttaaggctg atataccaga gaaaatttct
     5941 attagtaggg tagggaaaac tatcggctta cttgcattac tcgaaaaaca tctcgaccag
     6001 atgcctttaa caaaggctta tttggaatca gtcgttgaaa ctgtagaaga atttcagatg
     6061 cgccggatca agtgggcaat caagctgctt gatgaatgtg gagaagaaat tatgcgctgg
     6121 aaggtagtca gagttgctgc tttgcgagag gattgctccc aaagagtcag tgcatttctg
     6181 gaaagtgaac tggagaaagc atacaagaaa gagaggtgag cggccgcact cgaggcccga
     6241 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     6301 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca
     6361 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     6421 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
     6481 ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaaa
     6541 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     6601 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     6661 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     6721 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     6781 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     6841 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     6901 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     6961 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     7021 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     7081 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     7141 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     7201 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     7261 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     7321 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     7381 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     7441 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     7501 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     7561 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     7621 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7681 actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
     7741 cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     7801 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     7861 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     7921 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     7981 ctggtcccgc c
//
