LOCUS       Exported               14170 bp ds-DNA     circular SYN 06-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 6, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    74..109
                     /label=PmcCAST_DR
     misc_feature    110..144
                     /label=Spacer for PmcPSP1
     misc_feature    145..180
                     /label=PmcCAST_DR
     promoter        288..306
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    307..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             374..1036
                     /codon_start=1
                     /label=PmcCas6
                     /translation="MTAILIQSEEYVDLTFKLRGAPIPLDNGYLTYAALSRICPPLHEL
                     KSIGIHPIAGIPTRNNLLELTAQSRLKIRIYHQQIPLIYPYLAGQAFHIGQNFYQLDIP
                     DYKPLISSESVYSRLVIIKGFQDSTNFIEAVQRQMDNLGIQGKIELLTRQDGTPQRRQL
                     TINKEGKQFKVRGFGVKISELNPEDSLTLQEQGIGGKRKMMCGIFVPATRSKEEEET"
     CDS             1036..2616
                     /codon_start=1
                     /label=PmcCas8
                     /translation="MIVSTQPKISLSLHAADTTIMHRVGMTGLYMTLKRLEKQYPLSRQ
                     RGGHISWFLTADTIELFWEGSDFIALSWLINESFQLDDTGLIHLVGLDNDRIDLRQKIH
                     IHEGICGVFLRLNKFYQAGEIINTELRFEEKQVEYQYKSLTWYAHQTFAEKLCEADTQQ
                     LRHDYIQITSWLYLGGIVRHARTQNTTKLEEKPEYALALLFVPVVCHYCLLHIPSEDLK
                     ERKPHRYLVVIPEIKDFEDASQRRWRLQQLETKQFHVSSLGEAGLLYYSLDDIQPEVAY
                     YQACQVWLYEKTNKASRQRTLMSIEEIKIDKNILITYQQVQKYFKTNYQIIKYKQIFIK
                     VNPIRSLIADNLVKGIHWWSNFWEKLVIEDSKEYLFNQLFSNREGFIIMAENSEEDKQY
                     LIFIKVFQQAMKGNFAKIYAKTEEGKDPPIKKKVERLRAELNYCYDELSFKEYLSDFLV
                     RGGLNKYFNEHQEEIALLIKKSPWQEIRIWSLLAIASYKPKDKLTNRDDSSLSNNQKLE
                     EVNDDSEEE"
     CDS             2594..3565
                     /codon_start=1
                     /label=PmcCas7
                     /translation="MMTQKKNDSNIPNYYLYGTVLTRYGLASLNHDIRRGNKTILQKGY
                     WNNGKIHSFVGSSAIRWALRFYLQKQGYLVNRVWDEEEHINRLTSEDFDPEKFYDDDIF
                     GFALLESAETEEDTSTTKRKKKQTKTSTPNQRMGALGMNMAVSLTPYDGAVKLGAKSGR
                     EKDSTSLHFTEYHATRYQYYFGIDATHLKDFSRILPMIDGIMNLPKVGGSSNIFNYPFC
                     PDSLVFQWTNHFASYISYCFEYCDPKSKEAKLSQEFIDEVECGQIDPSKLWIGGTIVKD
                     LQQLDNFESSPLNKAHIYRNRNEMIEALKTVIKRDLGLEESK"
     CDS             3562..4200
                     /codon_start=1
                     /label=PmcCas5
                     /translation="MIAPLILYLDVPFTTFRESHAREMGKTYPVPPPATVYGMLLSLVG
                     ETNVYRHCGVELAIAMLSSPKKSRILRQMRRFKNADFSHPENVIPCYQEILSNLKCLIW
                     VRSDEEKIQPSLRERIQLAFDHPELVRRFGCLFLGESDQLIKTIKLAREDYLEGVRQWA
                     IRDNRGRLTLPYWVDHVGSRNTRFLRYRIEEMDRLSPPDLAWTMVQSPI"
     terminator      4230..4277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4442..4466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4510..7206
                     /codon_start=1
                     /label=PmcTnsAB
                     /translation="MKEQFEQWVARLQISEAGQKIIEKVRSSEPSRRVGGGSKNVSGRY
                     PSRKMGVTIQFESHRVELPIIYQLEHDKDVLEFYDQPPQIKLDYQASNGRRLGVLHTPD
                     LFVIRTNSAGWEECKTEQDLKKLAEKNPNRYFYSEENNQWHSLPGEVYANQFGLYHRIH
                     SDREINWVLQRNLQFLEDYYRSESLVVEEAIAQSLLAIVSSQPGITLAELLNRSTGAKS
                     DDIYTLIIQEQIYIDLNATPLAEPERCLIFRDEQTASAYRLMVEQPSVSLPAISPVVNI
                     VTGATVSWDGKGLNIIHVGETEVILGAENNQLIELKKAIFENLVRQGKITSLQTPEKTA
                     ISTESWQRFHQASPEDQAEALERYKTIEPYLNGHPPENETIPARTIRHWKAKYLIAIQK
                     YGCGYIGLLSHRSVKGNRQRKLPEDTLAIMEKFILEDYETLKQKRMWEVHAALVRACEE
                     AGVIAPSYKAFTKEVQRRTGYEQTKKRQGRRAAYQHESFYWELAITTPRHGDRPFEIGH
                     IDHTELDVELVCSDTGRNLGRPWATFLVDAYSRRLLAVYLTFDSPSYRSCLMVLRICVK
                     RHGRLPQIVVVDNGAEFHSVYFETLLATFECTKKQRPPAKARFGSVCERLFGTSNTQFV
                     HNLLGNTQITRNVRQVTKSVNPKNLAVWTLGLLYEYLCAWAYEVYDTDEHPALFQSPRD
                     AFAAGMAIGGSRVHRMIPYDENFQILTLPTTSEGKAKVQVGRGIKINYIYYWSNSFRDP
                     QIENTSVQIRYDPFNIGIAYAFVRGQWVQCISQYYAELQGRSEKELKLASIELRKHYSN
                     HTQQSKVSAKNLAEFLASVEAQEALLEQRSYDAEVKEVFRVIEGGKATTSRNEEPKLIQ
                     VTFANTDFQADEDEAIVPETLVVYEEF"
     CDS             7209..8360
                     /codon_start=1
                     /label=PmcTnsC
                     /translation="MTKSTGFPLELLTRPATERLAYFENYTVAHPRLKEVYEILMRTIA
                     EPAGASFIFVYGASGVGKTTLRLRVEQKLTELALPKLESDRARVPVVGIEAIAPESRYF
                     NWKEYYTRALITLEEPLIDHKFDYGVRGISRDNFGKINVESKVVAPALRRALENALIHR
                     HPDVFFVDEAQHFGKVASGYKLQDQLDCLKSLANMTGILHCLLGTYELLTFRNLSGQLS
                     RRSVDIHFRRYCADSPEDVQAFKSVLLTFQQHLPLAETPNLVDHWEYFYERTLGCIGTL
                     KDWLKRVLSDALDREATTITLKDLQKRALSVAQCQKMFKEIQEGERQLSETEADVQNLR
                     SALGLGAKPIVLPEETPKTTRPPGKVGKRKPKRDPIGVQQDVS"
     CDS             8350..9738
                     /codon_start=1
                     /label=PmcTnsD
                     /translation="MLASVLETYESWNLKKPLIPQRSRLYQPQPVGIGTPYIESLTGYI
                     TRIAELHGVLPGVLMTREIAPLVNKIYFQNGANRGFREIFNRSQALNGMGEMAADLVQV
                     LQKLTLRDDLRFLTMLFWSNILTPRNLFRTRKAWCPICYQERHQNGLVVYEQLLWTINL
                     ITICPQHQKPLVELCPHCNHESPLLNWRSRPGYCSKCGEWLGANQCLKTFTDGEGSIKL
                     QLEWQYWTANVVGELILASQCFESAPSKENITKSLNIVIDKVAENNAAAFSRLIGVPKN
                     SLWMWQSTKTLPELNTLLKICYELEISLVEFLTPKNLITKSFTKISQKHLQLSRTPRVS
                     PKSFDQYQVKDALLAILAGNEEPPPTMEEVGKRLGHHNRTISRHFPDLCSAISAKCRNY
                     NKACRLKSIEKLCSEVREIVLSLNAQGVYPTEGRVCELMPNPGCFRYKQVRAAFNDARR
                     EFGL"
     promoter        9790..9808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9809..9833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9874..10863
                     /codon_start=1
                     /label=PmcTniQ
                     /translation="MTIYSEHWSLNPLEIPQRSRLFSLEPVAVGTPYAESLSSYLHRLA
                     QAHCLTSEKLVMGEIAPLILKDEDKSELLSKNLSHLLGNSDAKPAINGMREMTEKLVTV
                     LEELTMRQDLRFLTLLSWKGMIYDKGLFRNYRAWCPCCCEEWMQKNKTIYEPLSWSFKD
                     VEFCLIHKQRLIEECSHCGARLPVMARLSPAGFCSRCYGWLGQEIKGEEEIEKYRVNIQ
                     GISELIALTPQLGYKPIPIELTRKLQLILLVFEQAIGKDVKLLGDLGGIMESLRIASTT
                     NQSQPYHLVKLIIPVCEKAKISVFQLFGSDFKELGKILFGNFSLELKL"
     terminator      10901..10948
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(11069..11860)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(11861..11952)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12000..12738)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(12948..14030)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(14031..14108)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(14154..14170,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtgagtc gtggctttat gccgttaggc gttgctcaag agaagtcatt
      121 taataaggcc actgttaaac gtaggtgagt cgtggcttta tgccgttagg cgttgctcaa
      181 gatccgaatt cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta
      241 agtcgaacag aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac
      301 tataggggaa ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga
      361 aggagatata catatgacag ctatattaat tcaaagtgag gaatatgtag acttaacatt
      421 taagctaaga ggcgcaccga ttcctcttga caacggttat cttacctacg ctgccttatc
      481 ccgtatttgt cccccactac atgaactaaa gtctattggg attcatccga ttgctggaat
      541 accgaccagg aacaatttgc ttgagctaac tgctcaatct cgtctaaaaa ttcggattta
      601 tcatcagcaa attcctttaa tttatcctta tttagcgggt caagcttttc atataggtca
      661 aaatttttat caactagata tccccgacta caaaccgtta atttcttcag aaagtgtcta
      721 ttccagatta gtgataatta aagggttcca agactctact aactttattg aagctgtgca
      781 acggcaaatg gataatttag gaatacaagg aaaaattgaa cttcttaccc gacaagatgg
      841 aacacctcaa cgaagacaat taaccatcaa taaagaaggg aaacagttta aagttagagg
      901 atttggcgta aaaatcagtg aactcaatcc tgaagattcc ttaaccttgc aagaacaggg
      961 aattggcggg aaacgaaaga tgatgtgtgg aatatttgtc ccagcgactc gcagcaagga
     1021 agaagaggaa acctgatgat agtctctacc caacccaaaa tttccctatc tctccatgct
     1081 gcggatacga caataatgca ccgcgtcgga atgaccggac tttacatgac tttaaagcga
     1141 ttggaaaagc aatatccctt atctcgtcaa cggggaggac atatatcatg gtttttgact
     1201 gctgatacta ttgaattatt ctgggaagga agtgatttca tcgccttatc ttggttaatt
     1261 aatgagtctt ttcaattaga tgatacaggt ttaattcatt tagtaggatt agacaatgat
     1321 cgaatagatt taaggcagaa aatccatatt catgagggaa tatgcggtgt ttttctacgt
     1381 cttaataagt tttatcaagc aggagagata attaacactg aactaaggtt tgaagaaaaa
     1441 caagtcgagt atcaatataa atccctaact tggtacgcac atcaaacctt tgcagagaag
     1501 ttatgcgagg cagataccca acaactcaga catgattaca ttcaaataac cagttggttg
     1561 tatttagggg gaattgttcg ccatgctcgg acgcagaata ctacaaaact agaagagaaa
     1621 cctgaatacg ctttagcatt attatttgta ccagtagttt gtcattattg cttacttcat
     1681 ataccatcag aagacttaaa agaaaggaaa ccccatcgct acttggtggt gattccagaa
     1741 ataaaggatt ttgaagacgc ttctcaaagg agatggcgat tacagcaatt agaaactaaa
     1801 cagtttcatg ttagtagcct tggggaagca ggattacttt attacagctt agacgatatt
     1861 cagcctgaag tggcctacta tcaagcttgt caagtttggt tatatgaaaa aacaaataaa
     1921 gcttctcgtc aacggacatt gatgagtata gaagaaatta aaattgataa gaatatttta
     1981 atcacttatc aacaggtgca aaaatacttt aaaactaatt atcagataat taaatataag
     2041 caaattttta ttaaggtcaa tccgattcgc tccctgattg ctgataattt agtgaaagga
     2101 atccattggt ggtctaattt ttgggaaaaa ttggtgatag aggattcaaa agaatattta
     2161 tttaaccagt tattttccaa tcgagaagga ttcatcataa tggcagagaa tagtgaagaa
     2221 gacaagcagt atcttatttt tattaaggta tttcagcaag ctatgaaggg caactttgct
     2281 aaaatttatg ccaaaacaga ggaaggtaaa gatcctccaa ttaagaagaa agttgaacga
     2341 ttaagggcag agctaaacta ttgttatgat gaattgtcgt ttaaggaata tttgtctgac
     2401 tttttagtaa gagggggatt aaataagtat tttaatgagc atcaggaaga gattgcacta
     2461 ctaattaaaa aatcaccttg gcaagaaata agaatttggt cattgttagc gatcgccagt
     2521 tataaaccca aagataaact cactaatagg gatgatagtt cattgtcaaa caatcaaaaa
     2581 ctagaagaag tgaatgatga ctcagaagaa gaatgacagt aatatcccaa attattatct
     2641 ttacggaaca gtcctcactc gttatggttt agcttcctta aatcatgaca ttagacgagg
     2701 aaataagact attctgcaaa aaggctattg gaataatggt aaaattcatt cttttgtagg
     2761 ttcaagtgca attcgttggg cgttacgttt ttatcttcaa aagcaaggtt atttagttaa
     2821 tcgagtctgg gatgaggaag aacatattaa tcggttaaca agtgaagatt tcgatccaga
     2881 aaaattttat gatgatgata tttttgggtt tgctttatta gagtctgctg aaacagagga
     2941 ggacacttca acaacaaaga gaaaaaagaa acaaacaaag accagcactc ctaatcagcg
     3001 aatgggtgct ttagggatga atatggctgt ttcgctaacg ccttatgatg gtgcagttaa
     3061 gttaggggca aaaagtggca gagagaagga tagtacatct ctccatttta cagaatatca
     3121 tgcaactcga tatcaatatt attttggaat tgatgccact catctcaaag atttttcgcg
     3181 gattttacct atgatagatg ggattatgaa tctgcccaag gtaggaggga gtagtaatat
     3241 ttttaattat cctttctgtc ccgatagttt ggtatttcaa tggacaaatc attttgcttc
     3301 atatatttcc tattgctttg aatattgcga tcctaagagt aaggaagcaa aactttcaca
     3361 agagtttata gatgaagtgg aatgcggaca gattgaccct agtaaattgt ggattggggg
     3421 aacaattgtt aaggatttac agcaattgga taattttgaa agttcccctt tgaacaaggc
     3481 gcatatttat cgtaatcgaa atgaaatgat tgaagctttg aaaacagtca ttaaaagaga
     3541 tttgggcttg gaagaatcaa aatgattgca ccattaattc tctatttgga tgttccattt
     3601 acgacttttc gggagtccca tgcaagggaa atgggaaaaa cctatcctgt tcctcctcca
     3661 gcgacagtgt atgggatgtt gttgtctttg gtaggagaaa cgaatgtcta tcgccactgt
     3721 ggggtagaat tggccattgc aatgttatct agccccaaaa agtcgcgaat tttgcgtcaa
     3781 atgagacggt ttaagaatgc tgattttagc catccagaga atgttattcc ctgttatcaa
     3841 gaaattttgt ctaatctgaa gtgtttgatt tgggttcgtt ctgacgagga aaagatacaa
     3901 ccaagtttaa gggagaggat acagttagcg tttgaccatc ctgagctagt aagacggttt
     3961 ggttgtttat ttttgggaga aagtgaccag ttgattaaaa cgatcaaact tgcaagggaa
     4021 gattatctag agggagtgag acagtgggcg attagagata atcgaggcag gttaacttta
     4081 ccctattggg ttgaccatgt ggggtcaaga aatacccgat ttttgaggta tcggattgag
     4141 gaaatggata gattatcacc tcctgattta gcctggacaa tggttcaatc tccgatttga
     4201 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     4261 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     4321 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctcgg
     4381 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag
     4441 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg
     4501 agatatacca tgaaagagca atttgaacaa tgggttgctc gccttcaaat atccgaggca
     4561 gggcaaaaaa ttattgagaa agttcgctca tctgagccat cccgtcgcgt tggtggcggt
     4621 agcaagaatg tttcgggacg ctaccctagt cgcaagatgg gagtcacgat tcaatttgag
     4681 tctcatcggg ttgaactgcc aattatctat cagttagaac atgataaaga tgttctagaa
     4741 ttctacgatc aaccaccgca aattaaactt gactatcaag caagtaacgg acgtagatta
     4801 ggcgttttac acaccccaga tttatttgtc atccgaacca attcggctgg ttgggaagag
     4861 tgtaaaactg agcaggattt gaaaaaacta gcagagaaaa accccaatcg ttacttttac
     4921 tctgaagaaa ataatcaatg gcattccttg ccaggagaag tttatgctaa ccagtttggg
     4981 ctttatcacc gcattcattc agatcgcgaa atcaattggg ttctgcaacg taatttgcaa
     5041 tttttagaag attattatcg ctcagaatct ctagtagtgg aagaagcgat cgctcaatca
     5101 ttacttgcaa ttgtgtcatc tcaaccagga atcactttag cagaattact caatcggtca
     5161 acaggagcta aatctgacga tatctacacc ttaattattc aagagcaaat ctatattgat
     5221 ttaaatgcta caccactagc tgaaccagaa agatgtctaa tcttccgtga cgagcaaact
     5281 gctagtgctt atagattaat ggttgagcaa ccaagtgtaa gtcttccggc tatctctcct
     5341 gtagtcaata ttgttactgg agcaactgtt agttgggacg gcaaaggttt aaatatcatc
     5401 catgttggag aaacagaggt tatacttggc gcagaaaata atcagttaat tgaactcaaa
     5461 aaagctatat ttgagaatct ggttcgccaa ggaaaaatca caagtctcca aacaccagaa
     5521 aaaacagcta ttagcacaga atcttggcag cgatttcatc aagctagtcc tgaagaccaa
     5581 gcagaagccc ttgaacgcta caaaactatc gaaccttact taaacggaca tcctccagaa
     5641 aacgaaacaa taccagcccg taccattcgt cactggaaag ctaaatattt aattgcaatt
     5701 caaaaatacg gttgcggcta cattgggctt ttgtcccatc gcagtgtcaa aggtaatcga
     5761 cagcgcaagc tgcccgaaga caccttagca attatggaga agtttatttt agaagactat
     5821 gaaactctta aacaaaaaag aatgtgggag gttcacgcgg ctttagttcg agcttgcgaa
     5881 gaagctggtg taattgctcc gagctataaa gcatttacca aagaagtaca acgccggact
     5941 ggttacgaac agactaaaaa acgccaaggt cgtcgtgccg cttatcagca tgaatccttc
     6001 tattgggagc ttgctataac taccccaaga cacggcgatc gcccttttga aattgggcat
     6061 atagatcata ctgagttaga tgtagagcta gtttgctctg atacaggtcg caatcttggc
     6121 agaccttggg caacatttct cgtagatgct tattcacggc gattgctggc agtttaccta
     6181 acgtttgact caccctcata tcgttcttgc ttaatggtgc taagaatttg tgtcaagcgt
     6241 catggcaggc ttccccaaat tgtagtggta gataacggtg cagagtttca cagcgtttac
     6301 tttgaaacac tactagctac ttttgaatgt acaaaaaagc aaagaccccc agccaaagct
     6361 cgttttggtt cggtttgcga acgcctattt ggaacttcca atactcaatt tgtccataac
     6421 ttacttggta atacacaaat cactcgtaat gtccgccaag tcactaagtc agttaaccct
     6481 aaaaatctag ctgtatggac acttggttta ctttatgaat atctttgtgc ttgggcttac
     6541 gaagtttatg atacagacga acatccggca cttttccaga gtccgaggga tgcgtttgct
     6601 gcgggtatgg cgattggagg cagtcgggtt caccgcatga ttccttacga tgaaaacttt
     6661 cagattctta ccctacctac aacctcagag ggaaaagcaa aagttcaagt tggacgcggc
     6721 ataaaaatta actacatata ctactggtct aatagtttcc gcgatcctca aattgaaaat
     6781 acctctgtgc agataagata cgacccgttt aatattggca tcgcttacgc ctttgttcga
     6841 ggacagtggg tacagtgtat ctctcaatat tatgcagaac tccaaggtcg ttcagaaaaa
     6901 gaactgaagt tagctagtat cgagttacgc aaacactatt caaatcatac acaacaatct
     6961 aaagtttcgg ctaagaattt agcagaattt ttagcatcgg tagaagctca agaagctcta
     7021 ttagaacagc gttcctatga tgcagaagtt aaggaagtct ttagggtaat cgagggtgga
     7081 aaagcgacaa caagccgtaa cgaagaacca aaacttattc aagttacttt tgccaatacc
     7141 gattttcaag cggatgaaga tgaagcgatc gtcccagaaa cacttgtagt gtatgaggag
     7201 ttttaactat gacaaaatct actggatttc cgcttgaact cctcacacgg cctgctacag
     7261 aaaggcttgc ttactttgaa aattacaccg tagcgcatcc tcgacttaaa gaagtctatg
     7321 aaattctcat gcgaacgatt gccgaaccag caggagcttc atttatcttt gtttacggtg
     7381 ctagtggtgt tggtaaaaca actttgcgac tccgcgttga gcagaaatta acagaactag
     7441 cattacctaa attggaaagc gatcgcgctc gtgttcctgt tgttggtatt gaagctatag
     7501 cgccagaatc gagatacttc aactggaaag aatactacac acgcgcctta attactttgg
     7561 aagaaccatt aatcgatcac aagtttgatt acggtgtgcg tggtatctct cgcgataatt
     7621 ttggcaaaat taatgttgag tcgaaagtag ttgcacctgc tctacgcaga gctttagaaa
     7681 atgctctcat ccatcgtcat ccagacgtat tttttgtaga tgaagcacaa cattttggta
     7741 aggtggcaag cggatacaaa ctacaagatc aattagactg tctcaaatct ttagcaaata
     7801 tgacaggcat cttgcactgt ctattaggaa catacgaact acttacattc cgtaatttga
     7861 gcggtcaact ctcacgtcgt agtgttgata ttcatttccg ccgttattgt gctgatagcc
     7921 cagaagatgt acaggctttt aagagtgtgt tgttaacttt tcaacagcat ctgccactag
     7981 cagaaactcc aaatttagtc gatcattggg aatattttta cgaacgtact ttaggatgta
     8041 ttggcacact gaaagattgg ctgaagcgtg ttcttagtga tgctttggat cgagaagcaa
     8101 caacaatcac tctcaaagat ttacaaaaac gtgctttgtc ggttgctcag tgtcagaaaa
     8161 tgttcaagga aattcaagag ggtgaacgac aattatctga aacagaagca gatgtgcaaa
     8221 acttacgctc tgcattagga cttggcgcaa aaccaattgt gctacccgaa gaaaccccaa
     8281 aaactactcg tccgccagga aaggttggca aacgcaaacc taaaagagat cccatcggag
     8341 tgcagcaaga tgttagctag tgtattagaa acatacgaat cttggaattt aaaaaagcca
     8401 ttaattccac aacgtagtcg cctctatcaa cctcaacctg taggtattgg gactccttac
     8461 atagagagtt taacaggtta tatcacacgg atagcagagt tgcatggagt tcttccagga
     8521 gtcttgatga ctagagaaat tgctccgctt gtcaataaaa tatatttcca aaatggagct
     8581 aatcgaggtt ttcgtgaaat attcaaccgt agtcaagcgt taaatggcat gggagagatg
     8641 gcagcagatt tagttcaagt gttgcaaaaa ttgacgctta gagatgactt gcgtttttta
     8701 accatgctgt tttggtctaa cattttgact ccacgcaatt tatttcgtac cagaaaagct
     8761 tggtgtccaa tttgttatca agagcggcat caaaatgggc tggtagtcta tgagcaattg
     8821 ctttggacaa ttaacctaat tacaatttgc ccgcagcatc aaaaaccctt ggttgaactt
     8881 tgccctcact gtaatcatga atcacctttg ttaaactggc gctcaagacc tggttactgt
     8941 tcaaagtgtg gagagtggtt aggcgcaaat caatgcctta agacatttac agatggtgaa
     9001 ggatcaataa aattacagtt ggagtggcag tattggactg ccaatgttgt aggagagcta
     9061 attttagctt ctcaatgctt tgaatctgcc ccatcaaaag aaaatattac aaaatcatta
     9121 aatatagtta ttgataaagt tgcggaaaat aatgcagctg cattttctcg tcttattgga
     9181 gtaccaaaaa atagtttgtg gatgtggcag tctactaaaa ccttgccaga gctaaataca
     9241 cttctgaaaa tttgttacga attagagata tcgttggtag aatttcttac tccaaaaaat
     9301 ctaattacta aatcttttac aaaaatctcg cagaagcacc tccaactttc tcgcacacca
     9361 agggtatctc caaaatcttt cgaccaatat caagtaaaag atgctttatt agcaatactt
     9421 gcaggtaatg aagaaccacc accaacaatg gaagaagttg gaaagcgttt aggtcatcac
     9481 aacagaacta tttctagaca ctttccagat ttgtgtagtg ctatttctgc taaatgtcgt
     9541 aattacaata aagcttgccg tctcaaaagc atagaaaaac tttgcagcga agttagagag
     9601 attgtcctta gccttaatgc tcagggtgtg tatcccacag aaggtcgtgt ttgtgaatta
     9661 atgccaaatc cggggtgttt tagatataaa caagtccgcg ctgcttttaa tgatgcacga
     9721 cgtgagtttg gtttgtaact taagtcgaac agaaagtaat cgtattgtac acggccgcat
     9781 aatcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccatctt
     9841 agtatattag ttaagtataa gaaggagata tacatgacta tttactcaga acattggagc
     9901 ctaaatcctc tagaaatccc gcaacggagt cggttatttt ctcttgaacc tgtggcagtg
     9961 ggaactccct atgcagaaag tcttagtagc tatctccatc gtttagcgca ggcgcattgt
    10021 ttaacctctg agaagctcgt tatgggagaa attgcgccac ttattctcaa agatgaggat
    10081 aaatctgagc tattgtcaaa aaatctgagt cacctattgg gaaatagcga cgctaaaccg
    10141 gctattaacg gaatgcgaga aatgacagaa aagttagtca ctgtcttaga agagttaaca
    10201 atgcgtcagg atttacgctt tttgaccctt ttaagctgga aaggaatgat ttatgacaag
    10261 ggattatttc ggaactatcg cgcttggtgt ccttgttgtt gtgaagagtg gatgcagaag
    10321 aacaaaacta tctatgaacc attatcatgg tcatttaagg acgtagaatt ttgtttgatt
    10381 cacaaacagc gattaataga agaatgttct cattgtggtg cccgtttacc tgtgatggct
    10441 aggctttcac ctgcgggatt ttgttctcgt tgttacgggt ggttaggaca ggaaattaaa
    10501 ggcgaagaag aaatagagaa atatcgagtt aatattcaag gaattagcga attaattgca
    10561 cttactcccc aattaggtta taaacccatc ccgatcgagt taacccgaaa attacaattg
    10621 attttgttag tgtttgaaca agcaattgga aaggatgtga agttattggg agatttagga
    10681 ggaatcatgg aatcgttgag gatagcctca acaacaaatc aaagtcaacc ttatcatttg
    10741 gtgaagttga ttattcctgt gtgcgagaag gcgaagatta gtgtattcca gttgtttggg
    10801 tcagatttta aggaattggg caagatattg ttcgggaatt ttagtctgga attaaagtta
    10861 tagttaatta acctaggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
    10921 ctctaaacgg gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt
    10981 cacactgctt ccggtagtgc cctgaaccga cgaccgggtc atcgtggccg gatcttgcgg
    11041 cccctcggct tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt
    11101 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
    11161 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
    11221 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
    11281 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
    11341 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
    11401 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
    11461 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
    11521 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
    11581 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
    11641 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
    11701 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
    11761 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
    11821 atagttgagt cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt
    11881 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11941 aaaataaaca aatagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg
    12001 cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt
    12061 gaggcaaaac agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag
    12121 agttcacata aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga
    12181 atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc
    12241 gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt
    12301 ctcccttacg ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg
    12361 taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc
    12421 gccttatccg gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg
    12481 gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc
    12541 tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct
    12601 gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca
    12661 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
    12721 caagaagatc ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct
    12781 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
    12841 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
    12901 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
    12961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    13021 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
    13081 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
    13141 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
    13201 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
    13261 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
    13321 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
    13381 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
    13441 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
    13501 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
    13561 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
    13621 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
    13681 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
    13741 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
    13801 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
    13861 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
    13921 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
    13981 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
    14041 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
    14101 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga aattaatacg
    14161 actcactata
//
