LOCUS       Exported               12792 bp ds-DNA     circular SYN 07-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    74..109
                     /label=PmcCAST_DR
     misc_feature    110..144
                     /label=Spacer for PmcPSP1
     misc_feature    145..180
                     /label=PmcCAST_DR
     promoter        288..306
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    307..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             374..1036
                     /codon_start=1
                     /label=PmcCas6
                     /translation="MTAILIQSEEYVDLTFKLRGAPIPLDNGYLTYAALSRICPPLHEL
                     KSIGIHPIAGIPTRNNLLELTAQSRLKIRIYHQQIPLIYPYLAGQAFHIGQNFYQLDIP
                     DYKPLISSESVYSRLVIIKGFQDSTNFIEAVQRQMDNLGIQGKIELLTRQDGTPQRRQL
                     TINKEGKQFKVRGFGVKISELNPEDSLTLQEQGIGGKRKMMCGIFVPATRSKEEEET"
     CDS             1036..2616
                     /codon_start=1
                     /label=PmcCas8
                     /translation="MIVSTQPKISLSLHAADTTIMHRVGMTGLYMTLKRLEKQYPLSRQ
                     RGGHISWFLTADTIELFWEGSDFIALSWLINESFQLDDTGLIHLVGLDNDRIDLRQKIH
                     IHEGICGVFLRLNKFYQAGEIINTELRFEEKQVEYQYKSLTWYAHQTFAEKLCEADTQQ
                     LRHDYIQITSWLYLGGIVRHARTQNTTKLEEKPEYALALLFVPVVCHYCLLHIPSEDLK
                     ERKPHRYLVVIPEIKDFEDASQRRWRLQQLETKQFHVSSLGEAGLLYYSLDDIQPEVAY
                     YQACQVWLYEKTNKASRQRTLMSIEEIKIDKNILITYQQVQKYFKTNYQIIKYKQIFIK
                     VNPIRSLIADNLVKGIHWWSNFWEKLVIEDSKEYLFNQLFSNREGFIIMAENSEEDKQY
                     LIFIKVFQQAMKGNFAKIYAKTEEGKDPPIKKKVERLRAELNYCYDELSFKEYLSDFLV
                     RGGLNKYFNEHQEEIALLIKKSPWQEIRIWSLLAIASYKPKDKLTNRDDSSLSNNQKLE
                     EVNDDSEEE"
     CDS             2594..3565
                     /codon_start=1
                     /label=PmcCas7
                     /translation="MMTQKKNDSNIPNYYLYGTVLTRYGLASLNHDIRRGNKTILQKGY
                     WNNGKIHSFVGSSAIRWALRFYLQKQGYLVNRVWDEEEHINRLTSEDFDPEKFYDDDIF
                     GFALLESAETEEDTSTTKRKKKQTKTSTPNQRMGALGMNMAVSLTPYDGAVKLGAKSGR
                     EKDSTSLHFTEYHATRYQYYFGIDATHLKDFSRILPMIDGIMNLPKVGGSSNIFNYPFC
                     PDSLVFQWTNHFASYISYCFEYCDPKSKEAKLSQEFIDEVECGQIDPSKLWIGGTIVKD
                     LQQLDNFESSPLNKAHIYRNRNEMIEALKTVIKRDLGLEESK"
     CDS             3562..4200
                     /codon_start=1
                     /label=PmcCas5
                     /translation="MIAPLILYLDVPFTTFRESHAREMGKTYPVPPPATVYGMLLSLVG
                     ETNVYRHCGVELAIAMLSSPKKSRILRQMRRFKNADFSHPENVIPCYQEILSNLKCLIW
                     VRSDEEKIQPSLRERIQLAFDHPELVRRFGCLFLGESDQLIKTIKLAREDYLEGVRQWA
                     IRDNRGRLTLPYWVDHVGSRNTRFLRYRIEEMDRLSPPDLAWTMVQSPI"
     terminator      4230..4277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4423..4441
                     /label=T7 promoter
     protein_bind    4442..4466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4510..7206
                     /codon_start=1
                     /label=PmcTnsAB
                     /translation="MKEQFEQWVARLQISEAGQKIIEKVRSSEPSRRVGGGSKNVSGRY
                     PSRKMGVTIQFESHRVELPIIYQLEHDKDVLEFYDQPPQIKLDYQASNGRRLGVLHTPD
                     LFVIRTNSAGWEECKTEQDLKKLAEKNPNRYFYSEENNQWHSLPGEVYANQFGLYHRIH
                     SDREINWVLQRNLQFLEDYYRSESLVVEEAIAQSLLAIVSSQPGITLAELLNRSTGAKS
                     DDIYTLIIQEQIYIDLNATPLAEPERCLIFRDEQTASAYRLMVEQPSVSLPAISPVVNI
                     VTGATVSWDGKGLNIIHVGETEVILGAENNQLIELKKAIFENLVRQGKITSLQTPEKTA
                     ISTESWQRFHQASPEDQAEALERYKTIEPYLNGHPPENETIPARTIRHWKAKYLIAIQK
                     YGCGYIGLLSHRSVKGNRQRKLPEDTLAIMEKFILEDYETLKQKRMWEVHAALVRACEE
                     AGVIAPSYKAFTKEVQRRTGYEQTKKRQGRRAAYQHESFYWELAITTPRHGDRPFEIGH
                     IDHTELDVELVCSDTGRNLGRPWATFLVDAYSRRLLAVYLTFDSPSYRSCLMVLRICVK
                     RHGRLPQIVVVDNGAEFHSVYFETLLATFECTKKQRPPAKARFGSVCERLFGTSNTQFV
                     HNLLGNTQITRNVRQVTKSVNPKNLAVWTLGLLYEYLCAWAYEVYDTDEHPALFQSPRD
                     AFAAGMAIGGSRVHRMIPYDENFQILTLPTTSEGKAKVQVGRGIKINYIYYWSNSFRDP
                     QIENTSVQIRYDPFNIGIAYAFVRGQWVQCISQYYAELQGRSEKELKLASIELRKHYSN
                     HTQQSKVSAKNLAEFLASVEAQEALLEQRSYDAEVKEVFRVIEGGKATTSRNEEPKLIQ
                     VTFANTDFQADEDEAIVPETLVVYEEF"
     CDS             7209..8360
                     /codon_start=1
                     /label=PmcTnsC
                     /translation="MTKSTGFPLELLTRPATERLAYFENYTVAHPRLKEVYEILMRTIA
                     EPAGASFIFVYGASGVGKTTLRLRVEQKLTELALPKLESDRARVPVVGIEAIAPESRYF
                     NWKEYYTRALITLEEPLIDHKFDYGVRGISRDNFGKINVESKVVAPALRRALENALIHR
                     HPDVFFVDEAQHFGKVASGYKLQDQLDCLKSLANMTGILHCLLGTYELLTFRNLSGQLS
                     RRSVDIHFRRYCADSPEDVQAFKSVLLTFQQHLPLAETPNLVDHWEYFYERTLGCIGTL
                     KDWLKRVLSDALDREATTITLKDLQKRALSVAQCQKMFKEIQEGERQLSETEADVQNLR
                     SALGLGAKPIVLPEETPKTTRPPGKVGKRKPKRDPIGVQQDVS"
     promoter        8412..8430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8431..8455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8496..9485
                     /codon_start=1
                     /label=PmcTniQ
                     /translation="MTIYSEHWSLNPLEIPQRSRLFSLEPVAVGTPYAESLSSYLHRLA
                     QAHCLTSEKLVMGEIAPLILKDEDKSELLSKNLSHLLGNSDAKPAINGMREMTEKLVTV
                     LEELTMRQDLRFLTLLSWKGMIYDKGLFRNYRAWCPCCCEEWMQKNKTIYEPLSWSFKD
                     VEFCLIHKQRLIEECSHCGARLPVMARLSPAGFCSRCYGWLGQEIKGEEEIEKYRVNIQ
                     GISELIALTPQLGYKPIPIELTRKLQLILLVFEQAIGKDVKLLGDLGGIMESLRIASTT
                     NQSQPYHLVKLIIPVCEKAKISVFQLFGSDFKELGKILFGNFSLELKL"
     terminator      9523..9570
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9691..10482)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(10483..10574)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10622..11360)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(11570..12652)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(12653..12730)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(12776..12792,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtgagtc gtggctttat gccgttaggc gttgctcaag agaagtcatt
      121 taataaggcc actgttaaac gtaggtgagt cgtggcttta tgccgttagg cgttgctcaa
      181 gatccgaatt cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta
      241 agtcgaacag aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac
      301 tataggggaa ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga
      361 aggagatata catatgacag ctatattaat tcaaagtgag gaatatgtag acttaacatt
      421 taagctaaga ggcgcaccga ttcctcttga caacggttat cttacctacg ctgccttatc
      481 ccgtatttgt cccccactac atgaactaaa gtctattggg attcatccga ttgctggaat
      541 accgaccagg aacaatttgc ttgagctaac tgctcaatct cgtctaaaaa ttcggattta
      601 tcatcagcaa attcctttaa tttatcctta tttagcgggt caagcttttc atataggtca
      661 aaatttttat caactagata tccccgacta caaaccgtta atttcttcag aaagtgtcta
      721 ttccagatta gtgataatta aagggttcca agactctact aactttattg aagctgtgca
      781 acggcaaatg gataatttag gaatacaagg aaaaattgaa cttcttaccc gacaagatgg
      841 aacacctcaa cgaagacaat taaccatcaa taaagaaggg aaacagttta aagttagagg
      901 atttggcgta aaaatcagtg aactcaatcc tgaagattcc ttaaccttgc aagaacaggg
      961 aattggcggg aaacgaaaga tgatgtgtgg aatatttgtc ccagcgactc gcagcaagga
     1021 agaagaggaa acctgatgat agtctctacc caacccaaaa tttccctatc tctccatgct
     1081 gcggatacga caataatgca ccgcgtcgga atgaccggac tttacatgac tttaaagcga
     1141 ttggaaaagc aatatccctt atctcgtcaa cggggaggac atatatcatg gtttttgact
     1201 gctgatacta ttgaattatt ctgggaagga agtgatttca tcgccttatc ttggttaatt
     1261 aatgagtctt ttcaattaga tgatacaggt ttaattcatt tagtaggatt agacaatgat
     1321 cgaatagatt taaggcagaa aatccatatt catgagggaa tatgcggtgt ttttctacgt
     1381 cttaataagt tttatcaagc aggagagata attaacactg aactaaggtt tgaagaaaaa
     1441 caagtcgagt atcaatataa atccctaact tggtacgcac atcaaacctt tgcagagaag
     1501 ttatgcgagg cagataccca acaactcaga catgattaca ttcaaataac cagttggttg
     1561 tatttagggg gaattgttcg ccatgctcgg acgcagaata ctacaaaact agaagagaaa
     1621 cctgaatacg ctttagcatt attatttgta ccagtagttt gtcattattg cttacttcat
     1681 ataccatcag aagacttaaa agaaaggaaa ccccatcgct acttggtggt gattccagaa
     1741 ataaaggatt ttgaagacgc ttctcaaagg agatggcgat tacagcaatt agaaactaaa
     1801 cagtttcatg ttagtagcct tggggaagca ggattacttt attacagctt agacgatatt
     1861 cagcctgaag tggcctacta tcaagcttgt caagtttggt tatatgaaaa aacaaataaa
     1921 gcttctcgtc aacggacatt gatgagtata gaagaaatta aaattgataa gaatatttta
     1981 atcacttatc aacaggtgca aaaatacttt aaaactaatt atcagataat taaatataag
     2041 caaattttta ttaaggtcaa tccgattcgc tccctgattg ctgataattt agtgaaagga
     2101 atccattggt ggtctaattt ttgggaaaaa ttggtgatag aggattcaaa agaatattta
     2161 tttaaccagt tattttccaa tcgagaagga ttcatcataa tggcagagaa tagtgaagaa
     2221 gacaagcagt atcttatttt tattaaggta tttcagcaag ctatgaaggg caactttgct
     2281 aaaatttatg ccaaaacaga ggaaggtaaa gatcctccaa ttaagaagaa agttgaacga
     2341 ttaagggcag agctaaacta ttgttatgat gaattgtcgt ttaaggaata tttgtctgac
     2401 tttttagtaa gagggggatt aaataagtat tttaatgagc atcaggaaga gattgcacta
     2461 ctaattaaaa aatcaccttg gcaagaaata agaatttggt cattgttagc gatcgccagt
     2521 tataaaccca aagataaact cactaatagg gatgatagtt cattgtcaaa caatcaaaaa
     2581 ctagaagaag tgaatgatga ctcagaagaa gaatgacagt aatatcccaa attattatct
     2641 ttacggaaca gtcctcactc gttatggttt agcttcctta aatcatgaca ttagacgagg
     2701 aaataagact attctgcaaa aaggctattg gaataatggt aaaattcatt cttttgtagg
     2761 ttcaagtgca attcgttggg cgttacgttt ttatcttcaa aagcaaggtt atttagttaa
     2821 tcgagtctgg gatgaggaag aacatattaa tcggttaaca agtgaagatt tcgatccaga
     2881 aaaattttat gatgatgata tttttgggtt tgctttatta gagtctgctg aaacagagga
     2941 ggacacttca acaacaaaga gaaaaaagaa acaaacaaag accagcactc ctaatcagcg
     3001 aatgggtgct ttagggatga atatggctgt ttcgctaacg ccttatgatg gtgcagttaa
     3061 gttaggggca aaaagtggca gagagaagga tagtacatct ctccatttta cagaatatca
     3121 tgcaactcga tatcaatatt attttggaat tgatgccact catctcaaag atttttcgcg
     3181 gattttacct atgatagatg ggattatgaa tctgcccaag gtaggaggga gtagtaatat
     3241 ttttaattat cctttctgtc ccgatagttt ggtatttcaa tggacaaatc attttgcttc
     3301 atatatttcc tattgctttg aatattgcga tcctaagagt aaggaagcaa aactttcaca
     3361 agagtttata gatgaagtgg aatgcggaca gattgaccct agtaaattgt ggattggggg
     3421 aacaattgtt aaggatttac agcaattgga taattttgaa agttcccctt tgaacaaggc
     3481 gcatatttat cgtaatcgaa atgaaatgat tgaagctttg aaaacagtca ttaaaagaga
     3541 tttgggcttg gaagaatcaa aatgattgca ccattaattc tctatttgga tgttccattt
     3601 acgacttttc gggagtccca tgcaagggaa atgggaaaaa cctatcctgt tcctcctcca
     3661 gcgacagtgt atgggatgtt gttgtctttg gtaggagaaa cgaatgtcta tcgccactgt
     3721 ggggtagaat tggccattgc aatgttatct agccccaaaa agtcgcgaat tttgcgtcaa
     3781 atgagacggt ttaagaatgc tgattttagc catccagaga atgttattcc ctgttatcaa
     3841 gaaattttgt ctaatctgaa gtgtttgatt tgggttcgtt ctgacgagga aaagatacaa
     3901 ccaagtttaa gggagaggat acagttagcg tttgaccatc ctgagctagt aagacggttt
     3961 ggttgtttat ttttgggaga aagtgaccag ttgattaaaa cgatcaaact tgcaagggaa
     4021 gattatctag agggagtgag acagtgggcg attagagata atcgaggcag gttaacttta
     4081 ccctattggg ttgaccatgt ggggtcaaga aatacccgat ttttgaggta tcggattgag
     4141 gaaatggata gattatcacc tcctgattta gcctggacaa tggttcaatc tccgatttga
     4201 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     4261 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     4321 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctcgg
     4381 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag
     4441 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg
     4501 agatatacca tgaaagagca atttgaacaa tgggttgctc gccttcaaat atccgaggca
     4561 gggcaaaaaa ttattgagaa agttcgctca tctgagccat cccgtcgcgt tggtggcggt
     4621 agcaagaatg tttcgggacg ctaccctagt cgcaagatgg gagtcacgat tcaatttgag
     4681 tctcatcggg ttgaactgcc aattatctat cagttagaac atgataaaga tgttctagaa
     4741 ttctacgatc aaccaccgca aattaaactt gactatcaag caagtaacgg acgtagatta
     4801 ggcgttttac acaccccaga tttatttgtc atccgaacca attcggctgg ttgggaagag
     4861 tgtaaaactg agcaggattt gaaaaaacta gcagagaaaa accccaatcg ttacttttac
     4921 tctgaagaaa ataatcaatg gcattccttg ccaggagaag tttatgctaa ccagtttggg
     4981 ctttatcacc gcattcattc agatcgcgaa atcaattggg ttctgcaacg taatttgcaa
     5041 tttttagaag attattatcg ctcagaatct ctagtagtgg aagaagcgat cgctcaatca
     5101 ttacttgcaa ttgtgtcatc tcaaccagga atcactttag cagaattact caatcggtca
     5161 acaggagcta aatctgacga tatctacacc ttaattattc aagagcaaat ctatattgat
     5221 ttaaatgcta caccactagc tgaaccagaa agatgtctaa tcttccgtga cgagcaaact
     5281 gctagtgctt atagattaat ggttgagcaa ccaagtgtaa gtcttccggc tatctctcct
     5341 gtagtcaata ttgttactgg agcaactgtt agttgggacg gcaaaggttt aaatatcatc
     5401 catgttggag aaacagaggt tatacttggc gcagaaaata atcagttaat tgaactcaaa
     5461 aaagctatat ttgagaatct ggttcgccaa ggaaaaatca caagtctcca aacaccagaa
     5521 aaaacagcta ttagcacaga atcttggcag cgatttcatc aagctagtcc tgaagaccaa
     5581 gcagaagccc ttgaacgcta caaaactatc gaaccttact taaacggaca tcctccagaa
     5641 aacgaaacaa taccagcccg taccattcgt cactggaaag ctaaatattt aattgcaatt
     5701 caaaaatacg gttgcggcta cattgggctt ttgtcccatc gcagtgtcaa aggtaatcga
     5761 cagcgcaagc tgcccgaaga caccttagca attatggaga agtttatttt agaagactat
     5821 gaaactctta aacaaaaaag aatgtgggag gttcacgcgg ctttagttcg agcttgcgaa
     5881 gaagctggtg taattgctcc gagctataaa gcatttacca aagaagtaca acgccggact
     5941 ggttacgaac agactaaaaa acgccaaggt cgtcgtgccg cttatcagca tgaatccttc
     6001 tattgggagc ttgctataac taccccaaga cacggcgatc gcccttttga aattgggcat
     6061 atagatcata ctgagttaga tgtagagcta gtttgctctg atacaggtcg caatcttggc
     6121 agaccttggg caacatttct cgtagatgct tattcacggc gattgctggc agtttaccta
     6181 acgtttgact caccctcata tcgttcttgc ttaatggtgc taagaatttg tgtcaagcgt
     6241 catggcaggc ttccccaaat tgtagtggta gataacggtg cagagtttca cagcgtttac
     6301 tttgaaacac tactagctac ttttgaatgt acaaaaaagc aaagaccccc agccaaagct
     6361 cgttttggtt cggtttgcga acgcctattt ggaacttcca atactcaatt tgtccataac
     6421 ttacttggta atacacaaat cactcgtaat gtccgccaag tcactaagtc agttaaccct
     6481 aaaaatctag ctgtatggac acttggttta ctttatgaat atctttgtgc ttgggcttac
     6541 gaagtttatg atacagacga acatccggca cttttccaga gtccgaggga tgcgtttgct
     6601 gcgggtatgg cgattggagg cagtcgggtt caccgcatga ttccttacga tgaaaacttt
     6661 cagattctta ccctacctac aacctcagag ggaaaagcaa aagttcaagt tggacgcggc
     6721 ataaaaatta actacatata ctactggtct aatagtttcc gcgatcctca aattgaaaat
     6781 acctctgtgc agataagata cgacccgttt aatattggca tcgcttacgc ctttgttcga
     6841 ggacagtggg tacagtgtat ctctcaatat tatgcagaac tccaaggtcg ttcagaaaaa
     6901 gaactgaagt tagctagtat cgagttacgc aaacactatt caaatcatac acaacaatct
     6961 aaagtttcgg ctaagaattt agcagaattt ttagcatcgg tagaagctca agaagctcta
     7021 ttagaacagc gttcctatga tgcagaagtt aaggaagtct ttagggtaat cgagggtgga
     7081 aaagcgacaa caagccgtaa cgaagaacca aaacttattc aagttacttt tgccaatacc
     7141 gattttcaag cggatgaaga tgaagcgatc gtcccagaaa cacttgtagt gtatgaggag
     7201 ttttaactat gacaaaatct actggatttc cgcttgaact cctcacacgg cctgctacag
     7261 aaaggcttgc ttactttgaa aattacaccg tagcgcatcc tcgacttaaa gaagtctatg
     7321 aaattctcat gcgaacgatt gccgaaccag caggagcttc atttatcttt gtttacggtg
     7381 ctagtggtgt tggtaaaaca actttgcgac tccgcgttga gcagaaatta acagaactag
     7441 cattacctaa attggaaagc gatcgcgctc gtgttcctgt tgttggtatt gaagctatag
     7501 cgccagaatc gagatacttc aactggaaag aatactacac acgcgcctta attactttgg
     7561 aagaaccatt aatcgatcac aagtttgatt acggtgtgcg tggtatctct cgcgataatt
     7621 ttggcaaaat taatgttgag tcgaaagtag ttgcacctgc tctacgcaga gctttagaaa
     7681 atgctctcat ccatcgtcat ccagacgtat tttttgtaga tgaagcacaa cattttggta
     7741 aggtggcaag cggatacaaa ctacaagatc aattagactg tctcaaatct ttagcaaata
     7801 tgacaggcat cttgcactgt ctattaggaa catacgaact acttacattc cgtaatttga
     7861 gcggtcaact ctcacgtcgt agtgttgata ttcatttccg ccgttattgt gctgatagcc
     7921 cagaagatgt acaggctttt aagagtgtgt tgttaacttt tcaacagcat ctgccactag
     7981 cagaaactcc aaatttagtc gatcattggg aatattttta cgaacgtact ttaggatgta
     8041 ttggcacact gaaagattgg ctgaagcgtg ttcttagtga tgctttggat cgagaagcaa
     8101 caacaatcac tctcaaagat ttacaaaaac gtgctttgtc ggttgctcag tgtcagaaaa
     8161 tgttcaagga aattcaagag ggtgaacgac aattatctga aacagaagca gatgtgcaaa
     8221 acttacgctc tgcattagga cttggcgcaa aaccaattgt gctacccgaa gaaaccccaa
     8281 aaactactcg tccgccagga aaggttggca aacgcaaacc taaaagagat cccatcggag
     8341 tgcagcaaga tgttagctag cttaagtcga acagaaagta atcgtattgt acacggccgc
     8401 ataatcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attccccatc
     8461 ttagtatatt agttaagtat aagaaggaga tatacatgac tatttactca gaacattgga
     8521 gcctaaatcc tctagaaatc ccgcaacgga gtcggttatt ttctcttgaa cctgtggcag
     8581 tgggaactcc ctatgcagaa agtcttagta gctatctcca tcgtttagcg caggcgcatt
     8641 gtttaacctc tgagaagctc gttatgggag aaattgcgcc acttattctc aaagatgagg
     8701 ataaatctga gctattgtca aaaaatctga gtcacctatt gggaaatagc gacgctaaac
     8761 cggctattaa cggaatgcga gaaatgacag aaaagttagt cactgtctta gaagagttaa
     8821 caatgcgtca ggatttacgc tttttgaccc ttttaagctg gaaaggaatg atttatgaca
     8881 agggattatt tcggaactat cgcgcttggt gtccttgttg ttgtgaagag tggatgcaga
     8941 agaacaaaac tatctatgaa ccattatcat ggtcatttaa ggacgtagaa ttttgtttga
     9001 ttcacaaaca gcgattaata gaagaatgtt ctcattgtgg tgcccgttta cctgtgatgg
     9061 ctaggctttc acctgcggga ttttgttctc gttgttacgg gtggttagga caggaaatta
     9121 aaggcgaaga agaaatagag aaatatcgag ttaatattca aggaattagc gaattaattg
     9181 cacttactcc ccaattaggt tataaaccca tcccgatcga gttaacccga aaattacaat
     9241 tgattttgtt agtgtttgaa caagcaattg gaaaggatgt gaagttattg ggagatttag
     9301 gaggaatcat ggaatcgttg aggatagcct caacaacaaa tcaaagtcaa ccttatcatt
     9361 tggtgaagtt gattattcct gtgtgcgaga aggcgaagat tagtgtattc cagttgtttg
     9421 ggtcagattt taaggaattg ggcaagatat tgttcgggaa ttttagtctg gaattaaagt
     9481 tatagttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     9541 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
     9601 gtcacactgc ttccggtagt gccctgaacc gacgaccggg tcatcgtggc cggatcttgc
     9661 ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc
     9721 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
     9781 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     9841 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     9901 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     9961 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
    10021 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
    10081 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
    10141 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
    10201 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
    10261 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
    10321 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
    10381 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
    10441 tgatagttga gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata
    10501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10561 gaaaaataaa caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg
    10621 ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg ggactaacat
    10681 gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc
    10741 agagttcaca taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat
    10801 gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg
    10861 tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct
    10921 ttctccctta cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg
    10981 tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct
    11041 gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac
    11101 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
    11161 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
    11221 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
    11281 cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat
    11341 ctcaagaaga tcctttgatc ttttctactg aaccgctcta gatttcagtg caatttatct
    11401 cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt
    11461 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
    11521 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
    11581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11641 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
    11701 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
    11761 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
    11821 cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg
    11881 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
    11941 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
    12001 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
    12061 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
    12121 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
    12181 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
    12241 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
    12301 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
    12361 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
    12421 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
    12481 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
    12541 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
    12601 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
    12661 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
    12721 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata
    12781 cgactcacta ta
//
