LOCUS       Exported                8777 bp ds-DNA     circular UNA 07-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...6l-cyfg2ai
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: da58e375-cec9-414c-af14-f7f53b755a41 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1020..1211
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1313..1452
                     /label=bom
     rep_origin      complement(1620..2302)
                     /direction=LEFT
                     /label=ColE1 ori
                     /label=ColE1 origin
     CDS             complement(2397..3059)
                     /label=AmpR
     promoter        3775..3793
                     /label=T7 promoter
     misc_feature    3794..3818
                     /label=lac operator
     misc_feature    3865..4014
                     /label=6x His tag/ Twin Strep
     misc_feature    4015..4308
                     /label=Sumo
     CDS             4312..7005
                     /codon_start=1
                     /label=PmcTnsAB
                     /translation="KEQFEQWVARLQISEAGQKIIEKVRSSEPSRRVGGGSKNVSGRYP
                     SRKMGVTIQFESHRVELPIIYQLEHDKDVLEFYDQPPQIKLDYQASNGRRLGVLHTPDL
                     FVIRTNSAGWEECKTEQDLKKLAEKNPNRYFYSEENNQWHSLPGEVYANQFGLYHRIHS
                     DREINWVLQRNLQFLEDYYRSESLVVEEAIAQSLLAIVSSQPGITLAELLNRSTGAKSD
                     DIYTLIIQEQIYIDLNATPLAEPERCLIFRDEQTASAYRLMVEQPSVSLPAISPVVNIV
                     TGATVSWDGKGLNIIHVGETEVILGAENNQLIELKKAIFENLVRQGKITSLQTPEKTAI
                     STESWQRFHQASPEDQAEALERYKTIEPYLNGHPPENETIPARTIRHWKAKYLIAIQKY
                     GCGYIGLLSHRSVKGNRQRKLPEDTLAIMEKFILEDYETLKQKRMWEVHAALVRACEEA
                     GVIAPSYKAFTKEVQRRTGYEQTKKRQGRRAAYQHESFYWELAITTPRHGDRPFEIGHI
                     DHTELDVELVCSDTGRNLGRPWATFLVDAYSRRLLAVYLTFDSPSYRSCLMVLRICVKR
                     HGRLPQIVVVDNGAEFHSVYFETLLATFECTKKQRPPAKARFGSVCERLFGTSNTQFVH
                     NLLGNTQITRNVRQVTKSVNPKNLAVWTLGLLYEYLCAWAYEVYDTDEHPALFQSPRDA
                     FAAGMAIGGSRVHRMIPYDENFQILTLPTTSEGKAKVQVGRGIKINYIYYWSNSFRDPQ
                     IENTSVQIRYDPFNIGIAYAFVRGQWVQCISQYYAELQGRSEKELKLASIELRKHYSNH
                     TQQSKVSAKNLAEFLASVEAQEALLEQRSYDAEVKEVFRVIEGGKATTSRNEEPKLIQV
                     TFANTDFQADEDEAIVPETLVVYEEF"
     terminator      7066..7113
                     /label=T7 terminator
     promoter        7326..7402
                     /label=lacI promoter
     CDS             7403..8485
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaaccggg atctcgacgc
     3481 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3541 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     3601 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3661 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3721 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
     3781 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3841 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
     3901 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
     3961 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
     4021 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
     4081 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
     4141 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
     4201 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
     4261 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc caaagagcaa
     4321 tttgaacaat gggttgctcg ccttcaaata tccgaggcag ggcaaaaaat tattgagaaa
     4381 gttcgctcat ctgagccatc ccgtcgcgtt ggtggcggta gcaagaatgt ttcgggacgc
     4441 taccctagtc gcaagatggg agtcacgatt caatttgagt ctcatcgggt tgaactgcca
     4501 attatctatc agttagaaca tgataaagat gttctagaat tctacgatca accaccgcaa
     4561 attaaacttg actatcaagc aagtaacgga cgtagattag gcgttttaca caccccagat
     4621 ttatttgtca tccgaaccaa ttcggctggt tgggaagagt gtaaaactga gcaggatttg
     4681 aaaaaactag cagagaaaaa ccccaatcgt tacttttact ctgaagaaaa taatcaatgg
     4741 cattccttgc caggagaagt ttatgctaac cagtttgggc tttatcaccg cattcattca
     4801 gatcgcgaaa tcaattgggt tctgcaacgt aatttgcaat ttttagaaga ttattatcgc
     4861 tcagaatctc tagtagtgga agaagcgatc gctcaatcat tacttgcaat tgtgtcatct
     4921 caaccaggaa tcactttagc agaattactc aatcggtcaa caggagctaa atctgacgat
     4981 atctacacct taattattca agagcaaatc tatattgatt taaatgctac accactagct
     5041 gaaccagaaa gatgtctaat cttccgtgac gagcaaactg ctagtgctta tagattaatg
     5101 gttgagcaac caagtgtaag tcttccggct atctctcctg tagtcaatat tgttactgga
     5161 gcaactgtta gttgggacgg caaaggttta aatatcatcc atgttggaga aacagaggtt
     5221 atacttggcg cagaaaataa tcagttaatt gaactcaaaa aagctatatt tgagaatctg
     5281 gttcgccaag gaaaaatcac aagtctccaa acaccagaaa aaacagctat tagcacagaa
     5341 tcttggcagc gatttcatca agctagtcct gaagaccaag cagaagccct tgaacgctac
     5401 aaaactatcg aaccttactt aaacggacat cctccagaaa acgaaacaat accagcccgt
     5461 accattcgtc actggaaagc taaatattta attgcaattc aaaaatacgg ttgcggctac
     5521 attgggcttt tgtcccatcg cagtgtcaaa ggtaatcgac agcgcaagct gcccgaagac
     5581 accttagcaa ttatggagaa gtttatttta gaagactatg aaactcttaa acaaaaaaga
     5641 atgtgggagg ttcacgcggc tttagttcga gcttgcgaag aagctggtgt aattgctccg
     5701 agctataaag catttaccaa agaagtacaa cgccggactg gttacgaaca gactaaaaaa
     5761 cgccaaggtc gtcgtgccgc ttatcagcat gaatccttct attgggagct tgctataact
     5821 accccaagac acggcgatcg cccttttgaa attgggcata tagatcatac tgagttagat
     5881 gtagagctag tttgctctga tacaggtcgc aatcttggca gaccttgggc aacatttctc
     5941 gtagatgctt attcacggcg attgctggca gtttacctaa cgtttgactc accctcatat
     6001 cgttcttgct taatggtgct aagaatttgt gtcaagcgtc atggcaggct tccccaaatt
     6061 gtagtggtag ataacggtgc agagtttcac agcgtttact ttgaaacact actagctact
     6121 tttgaatgta caaaaaagca aagaccccca gccaaagctc gttttggttc ggtttgcgaa
     6181 cgcctatttg gaacttccaa tactcaattt gtccataact tacttggtaa tacacaaatc
     6241 actcgtaatg tccgccaagt cactaagtca gttaacccta aaaatctagc tgtatggaca
     6301 cttggtttac tttatgaata tctttgtgct tgggcttacg aagtttatga tacagacgaa
     6361 catccggcac ttttccagag tccgagggat gcgtttgctg cgggtatggc gattggaggc
     6421 agtcgggttc accgcatgat tccttacgat gaaaactttc agattcttac cctacctaca
     6481 acctcagagg gaaaagcaaa agttcaagtt ggacgcggca taaaaattaa ctacatatac
     6541 tactggtcta atagtttccg cgatcctcaa attgaaaata cctctgtgca gataagatac
     6601 gacccgttta atattggcat cgcttacgcc tttgttcgag gacagtgggt acagtgtatc
     6661 tctcaatatt atgcagaact ccaaggtcgt tcagaaaaag aactgaagtt agctagtatc
     6721 gagttacgca aacactattc aaatcataca caacaatcta aagtttcggc taagaattta
     6781 gcagaatttt tagcatcggt agaagctcaa gaagctctat tagaacagcg ttcctatgat
     6841 gcagaagtta aggaagtctt tagggtaatc gagggtggaa aagcgacaac aagccgtaac
     6901 gaagaaccaa aacttattca agttactttt gccaataccg attttcaagc ggatgaagat
     6961 gaagcgatcg tcccagaaac acttgtagtg tatgaggagt tttaagcggc cgcactcgag
     7021 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     7081 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
     7141 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     7201 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     7261 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
     7321 gagaaacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     7381 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     7441 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     7501 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     7561 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     7621 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     7681 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     7741 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     7801 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     7861 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     7921 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     7981 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     8041 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     8101 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     8161 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     8221 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     8281 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     8341 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     8401 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8461 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt
     8521 aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     8581 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     8641 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     8701 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     8761 tcgtcactgg tcccgcc
//
