LOCUS       Exported                7232 bp ds-DNA     circular UNA 07-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...6l-cyfg2ai
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: da58e375-cec9-414c-af14-f7f53b755a41 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1020..1211
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1313..1452
                     /label=bom
     rep_origin      complement(1620..2302)
                     /direction=LEFT
                     /label=ColE1 ori
                     /label=ColE1 origin
     CDS             complement(2397..3059)
                     /label=AmpR
     promoter        3775..3793
                     /label=T7 promoter
     misc_feature    3794..3818
                     /label=lac operator
     misc_feature    3865..4014
                     /label=6x His tag/ Twin Strep
     misc_feature    4015..4308
                     /label=Sumo
     CDS             4312..5460
                     /codon_start=1
                     /label=PmcTnsC
                     /translation="TKSTGFPLELLTRPATERLAYFENYTVAHPRLKEVYEILMRTIAE
                     PAGASFIFVYGASGVGKTTLRLRVEQKLTELALPKLESDRARVPVVGIEAIAPESRYFN
                     WKEYYTRALITLEEPLIDHKFDYGVRGISRDNFGKINVESKVVAPALRRALENALIHRH
                     PDVFFVDEAQHFGKVASGYKLQDQLDCLKSLANMTGILHCLLGTYELLTFRNLSGQLSR
                     RSVDIHFRRYCADSPEDVQAFKSVLLTFQQHLPLAETPNLVDHWEYFYERTLGCIGTLK
                     DWLKRVLSDALDREATTITLKDLQKRALSVAQCQKMFKEIQEGERQLSETEADVQNLRS
                     ALGLGAKPIVLPEETPKTTRPPGKVGKRKPKRDPIGVQQDVS"
     terminator      5521..5568
                     /label=T7 terminator
     promoter        5781..5857
                     /label=lacI promoter
     CDS             5858..6940
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaaccggg atctcgacgc
     3481 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3541 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     3601 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3661 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3721 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
     3781 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3841 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
     3901 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
     3961 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
     4021 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
     4081 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
     4141 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
     4201 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
     4261 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc cacaaaatct
     4321 actggatttc cgcttgaact cctcacacgg cctgctacag aaaggcttgc ttactttgaa
     4381 aattacaccg tagcgcatcc tcgacttaaa gaagtctatg aaattctcat gcgaacgatt
     4441 gccgaaccag caggagcttc atttatcttt gtttacggtg ctagtggtgt tggtaaaaca
     4501 actttgcgac tccgcgttga gcagaaatta acagaactag cattacctaa attggaaagc
     4561 gatcgcgctc gtgttcctgt tgttggtatt gaagctatag cgccagaatc gagatacttc
     4621 aactggaaag aatactacac acgcgcctta attactttgg aagaaccatt aatcgatcac
     4681 aagtttgatt acggtgtgcg tggtatctct cgcgataatt ttggcaaaat taatgttgag
     4741 tcgaaagtag ttgcacctgc tctacgcaga gctttagaaa atgctctcat ccatcgtcat
     4801 ccagacgtat tttttgtaga tgaagcacaa cattttggta aggtggcaag cggatacaaa
     4861 ctacaagatc aattagactg tctcaaatct ttagcaaata tgacaggcat cttgcactgt
     4921 ctattaggaa catacgaact acttacattc cgtaatttga gcggtcaact ctcacgtcgt
     4981 agtgttgata ttcatttccg ccgttattgt gctgatagcc cagaagatgt acaggctttt
     5041 aagagtgtgt tgttaacttt tcaacagcat ctgccactag cagaaactcc aaatttagtc
     5101 gatcattggg aatattttta cgaacgtact ttaggatgta ttggcacact gaaagattgg
     5161 ctgaagcgtg ttcttagtga tgctttggat cgagaagcaa caacaatcac tctcaaagat
     5221 ttacaaaaac gtgctttgtc ggttgctcag tgtcagaaaa tgttcaagga aattcaagag
     5281 ggtgaacgac aattatctga aacagaagca gatgtgcaaa acttacgctc tgcattagga
     5341 cttggcgcaa aaccaattgt gctacccgaa gaaaccccaa aaactactcg tccgccagga
     5401 aaggttggca aacgcaaacc taaaagagat cccatcggag tgcagcaaga tgttagctag
     5461 gcggccgcac tcgaggcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     5521 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     5581 actatatccg gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta
     5641 cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg
     5701 gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga
     5761 taagctgtca aacatgagaa acaccatcga atggcgcaaa acctttcgcg gtatggcatg
     5821 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     5881 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     5941 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     6001 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     6061 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     6121 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     6181 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     6241 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     6301 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     6361 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     6421 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     6481 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     6541 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     6601 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     6661 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     6721 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     6781 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     6841 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6901 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa
     6961 gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag
     7021 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     7081 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
     7141 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
     7201 ccctcgctca agccttcgtc actggtcccg cc
//
