LOCUS       Exported                7469 bp ds-DNA     circular UNA 07-MAR-2021
DEFINITION  natural circular DNA
ACCESSION   urn.local...6l-cyfg2ai
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: da58e375-cec9-414c-af14-f7f53b755a41 
            Sequence Type            :: Synthetic
            Other Notes              :: CreatedBy: Broad Institute 
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1020..1211
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1313..1452
                     /label=bom
     rep_origin      complement(1620..2302)
                     /direction=LEFT
                     /label=ColE1 ori
                     /label=ColE1 origin
     CDS             complement(2397..3059)
                     /label=AmpR
     promoter        3775..3793
                     /label=T7 promoter
     misc_feature    3794..3818
                     /label=lac operator
     misc_feature    3865..4014
                     /label=6x His tag/ Twin Strep
     misc_feature    4015..4308
                     /label=Sumo
     CDS             4312..5697
                     /codon_start=1
                     /label=PmcTnsD
                     /translation="LASVLETYESWNLKKPLIPQRSRLYQPQPVGIGTPYIESLTGYIT
                     RIAELHGVLPGVLMTREIAPLVNKIYFQNGANRGFREIFNRSQALNGMGEMAADLVQVL
                     QKLTLRDDLRFLTMLFWSNILTPRNLFRTRKAWCPICYQERHQNGLVVYEQLLWTINLI
                     TICPQHQKPLVELCPHCNHESPLLNWRSRPGYCSKCGEWLGANQCLKTFTDGEGSIKLQ
                     LEWQYWTANVVGELILASQCFESAPSKENITKSLNIVIDKVAENNAAAFSRLIGVPKNS
                     LWMWQSTKTLPELNTLLKICYELEISLVEFLTPKNLITKSFTKISQKHLQLSRTPRVSP
                     KSFDQYQVKDALLAILAGNEEPPPTMEEVGKRLGHHNRTISRHFPDLCSAISAKCRNYN
                     KACRLKSIEKLCSEVREIVLSLNAQGVYPTEGRVCELMPNPGCFRYKQVRAAFNDARRE
                     FGL"
     terminator      5758..5805
                     /label=T7 terminator
     promoter        6018..6094
                     /label=lacI promoter
     CDS             6095..7177
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
       61 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
      121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
      181 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
      241 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
      301 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
      361 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
      421 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
      481 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
      541 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
      601 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
      661 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
      721 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
      781 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
      841 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
      901 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
      961 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1081 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1141 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaaccggg atctcgacgc
     3481 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3541 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     3601 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3661 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3721 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
     3781 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3841 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
     3901 ctggtgccgc gcggcagcca tatggctagc tggagccatc cgcagtttga aaaaggtggt
     3961 ggtagcggtg gtggttcagg tggtagtgca tggtcacacc ctcagtttga gaaaatgtcg
     4021 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
     4081 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
     4141 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
     4201 ttaagattct tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac
     4261 atggaggata acgatattat tgaggctcac agagaacaga ttggtggatc cttagctagt
     4321 gtattagaaa catacgaatc ttggaattta aaaaagccat taattccaca acgtagtcgc
     4381 ctctatcaac ctcaacctgt aggtattggg actccttaca tagagagttt aacaggttat
     4441 atcacacgga tagcagagtt gcatggagtt cttccaggag tcttgatgac tagagaaatt
     4501 gctccgcttg tcaataaaat atatttccaa aatggagcta atcgaggttt tcgtgaaata
     4561 ttcaaccgta gtcaagcgtt aaatggcatg ggagagatgg cagcagattt agttcaagtg
     4621 ttgcaaaaat tgacgcttag agatgacttg cgttttttaa ccatgctgtt ttggtctaac
     4681 attttgactc cacgcaattt atttcgtacc agaaaagctt ggtgtccaat ttgttatcaa
     4741 gagcggcatc aaaatgggct ggtagtctat gagcaattgc tttggacaat taacctaatt
     4801 acaatttgcc cgcagcatca aaaacccttg gttgaacttt gccctcactg taatcatgaa
     4861 tcacctttgt taaactggcg ctcaagacct ggttactgtt caaagtgtgg agagtggtta
     4921 ggcgcaaatc aatgccttaa gacatttaca gatggtgaag gatcaataaa attacagttg
     4981 gagtggcagt attggactgc caatgttgta ggagagctaa ttttagcttc tcaatgcttt
     5041 gaatctgccc catcaaaaga aaatattaca aaatcattaa atatagttat tgataaagtt
     5101 gcggaaaata atgcagctgc attttctcgt cttattggag taccaaaaaa tagtttgtgg
     5161 atgtggcagt ctactaaaac cttgccagag ctaaatacac ttctgaaaat ttgttacgaa
     5221 ttagagatat cgttggtaga atttcttact ccaaaaaatc taattactaa atcttttaca
     5281 aaaatctcgc agaagcacct ccaactttct cgcacaccaa gggtatctcc aaaatctttc
     5341 gaccaatatc aagtaaaaga tgctttatta gcaatacttg caggtaatga agaaccacca
     5401 ccaacaatgg aagaagttgg aaagcgttta ggtcatcaca acagaactat ttctagacac
     5461 tttccagatt tgtgtagtgc tatttctgct aaatgtcgta attacaataa agcttgccgt
     5521 ctcaaaagca tagaaaaact ttgcagcgaa gttagagaga ttgtccttag ccttaatgct
     5581 cagggtgtgt atcccacaga aggtcgtgtt tgtgaattaa tgccaaatcc ggggtgtttt
     5641 agatataaac aagtccgcgc tgcttttaat gatgcacgac gtgagtttgg tttgtaagcg
     5701 gccgcactcg aggcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     5761 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     5821 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     5881 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     5941 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
     6001 gctgtcaaac atgagaaaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
     6061 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
     6121 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     6181 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     6241 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     6301 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     6361 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     6421 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     6481 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     6541 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     6601 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     6661 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     6721 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     6781 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     6841 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     6901 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     6961 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     7021 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     7081 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7141 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
     7201 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
     7261 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     7321 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     7381 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     7441 tcgctcaagc cttcgtcact ggtcccgcc
//
