LOCUS       pTH32-pmyo-3-YFP-CaCycl 9340 bp ds-DNA     circular     04-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..9340
                     /label="color: #ffffff"
     misc_feature    1..2380
                     /label="myo-3"
     CDS             2386..3102
                     /label="Venus YFP with monomerizing A206K mutation (Nag"
     misc_feature    3109..4995
                     /label="CaCyclOp"
     CDS             4603..4605
                     /label="E497K"
     CDS             4810..4812
                     /label="C566D"
     misc_feature    5006..5236
                     /label="SL2"
     CDS             5261..5971
                     /label="monomeric derivative of DsRed fluorescent prote"
     promoter        7196..7300
                     /label="bla promoter"
     CDS             7301..8161
                     /label="bla"
     rep_origin      8332..8920
                     /label="ori"
     protein_bind    9207..9228
                     /label="E. coli catabolite activator protein binding site"
     promoter        9243..9273
                     /label="lac promoter;promoter for the E. coli lac operon;color: #ffffff"
     protein_bind    9281..9297
                     /label="lac repressor encoded by lacI binding site"
     primer_bind     9305..9321
                     /label="M13 rev"
ORIGIN
        1 cggctataat aagttcttga ataaaataat tttcccgaca aaacatgagt atttctttcg
       61 aaaataaaag tgcaggctaa ttagagatta ttctgtaatt aactgcataa tttgtcacgt
      121 gccatagttt tacattccac tacgtcatag ttcttaaaat actaatctcc tgaaaataga
      181 agtaggtgaa gaaagtttaa ttatcagttc taaaatgaca attgatcttt ggaatatgtt
      241 ctgaaactac cgatcattga acagatgcta tttgaatgat atagaattgt atatttgcaa
      301 tttctgaaac gcgttcttaa aggcacacag attaattcaa aagggtctgg ccgcaaaaag
      361 gtttatggtg gccgattttg agttttgtgt gtgattgctt tttcacaatc agtgttttca
      421 ggattatgtg atgaactaga tcttcaagtt tcgttacatt tcatatgttt tcggaactca
      481 cgaagtacat attgggtatt gtgctcaaaa aattcagcaa tcagcttcgc tccgctgact
      541 ttagaaccca aaaaaatagt atggccaaac tgactgtgtt acgatcattt caatttttca
      601 atacatattt aagatttcta agagtaagaa ggtcaaaaac tgttctggaa tacatatata
      661 tttttcaggt tacaattagt caaaaagtgc actgaaatat acgttttaat ttcacgaata
      721 acccaattag ttcaatgtat ttttggtcaa ccaacgttaa agtttggctt ccaaccaatt
      781 atcatttctg atcaaccaca atgttttttc tttatctgca agttaatttt ttatttttat
      841 ccagatgttt ggcatatttt tcaattcttc actagcgccc acttcttgca cttccggcgc
      901 cctgaatcta atgcatctgt tgcaagaatt gaaagaccaa tcaacacatt gttttcttca
      961 cgagatactg aagaaaatga ataaaaacag agaaaaagag ccatgtgatt agtgacaact
     1021 gttgctaaca gataccatag cttggacttg gtacgtgatg gcaacgtatg ggtcaacaaa
     1081 aatgattgca gagggggtgc aaaacagtca agtcgagaaa atatgaaaaa cagaaaacaa
     1141 agaacagaaa aatgggtttg agagtcagta taatttataa aagaaaaatt gtacatagaa
     1201 attaaccatt tttgtagaag aagttatttt tcaagcatcg ttaaaaatta ttcaaagcac
     1261 cttatttcat atttaatttt aaacatggtt aaatgaacaa cacggtgcgc aatcaggaaa
     1321 acttgaaatc tgaaactgtt gttgtgatct tcttcgcaac tgttcagata gcactagtgt
     1381 aatgttaaga gtgcgcgaat ataatggaat ataatggatc acacctcctg ccatcaggta
     1441 aacgtctctg ttatcacata tttccaacta ttaaattttt accttttaca gttttacatt
     1501 tttttgaaaa aagtaacttt ttgtcttcaa aatccctgac gaaaatatca aatattttaa
     1561 tcgagactgc agaggaaccg attgatgatt tggaaaatcc agctttacct gtgtaagaac
     1621 tgaaaagttt cataacccta gggtattccc agttacattc cccactggct aacaatagca
     1681 cccagttttt catcaccttt cttcaaattt ctcggcgatt tgttaaaaac aaaatttgtg
     1741 tcccttctct gatatctcta tgtctctaaa cacaagttca tcggaaaacg aaggagggta
     1801 ggtgttggtt gggctcccga agtgaaaata gaagagcaag aatagaatat tagagagaga
     1861 gtgcagagag ggcgggatag ctcccgggat tccgttttct tcttctttat cttcaacgat
     1921 gatgtgtgtg cgtgttgtat agattctgtt gctcccccac aactcgctcc gaaggctcaa
     1981 tacaattcaa ttgatattgg aggagagcct accggagtgg gaggataaga agaaacataa
     2041 gaagaagaag aagaagaagc atgcttctgg tttttgatgc tatgaaaacg gcacaaaaag
     2101 atgattgagg tcccttttca ataccttctc tcatctttca aatcccattg aaacctaaaa
     2161 cttctcacca cgctttacca ttgttctcca aaaacttata gcaatgtcta taactttttt
     2221 atctctgaaa agcagtgttc catttttctt tttcctattt tatttcaatt gtttctcaca
     2281 tttcgtttgg attctttgct tgtcaaccag cttcttcttc cacttttacc gtctaatttt
     2341 cagggcaggg agccatcaaa cccacgacca ctagatccat ctagaatggt gagcaagggc
     2401 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2461 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2521 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2581 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     2641 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2701 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2761 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2821 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     2881 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     2941 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3001 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3061 accgccgccg ggatcactct cggcatggac gagctgtaca agggatcaat gtctatgaaa
     3121 gataaagata ataacctgcg gggagcatgt agtggctgtt cctgccctga atactgttac
     3181 tcaccaacct caaccctgtg cgacgattgc aagtgtagcg tgacaaaaca ccctatcgtc
     3241 gagcagccac tgactcgaaa cggaagtttc cggagctccg gagcctcact gctgccatct
     3301 cccagtcagc ccaatatcaa ggtcactggc tctagtaccg cctcaagcaa cgctaatatg
     3361 cggaacagac agaacaattc actgagcgtg tccaatgtcc gcagcacctc ctctgcaagt
     3421 tcaagcaacg tgtcctctcc cgccaatagc cgacctggct ctcctagtaa gcagtccgct
     3481 ctgcagcagt accagacaaa catcgcagac atgtggtcct gggatatgat gctgagtacc
     3541 ccttcactga aattcctgac aggacagttt atcatgtggg ccattctgac cgtggccggc
     3601 gctttctacg ctctgtttat ccaggaaaga caggcttata ataggggctg ggcagacatt
     3661 tggtacggct atggggcctt cggatttggc atcgggattg ctttcagtta tatgggattt
     3721 gcaggcgcca gaaacccaga gaagaaagca ctgtccctgt gcctgctggg agtgaacatc
     3781 atcgctttta gttcatacat cctgattatg ctgcggctga ctccaaccat cgaaggcacc
     3841 ctgagcaacc cagtggagcc tgcaagatac ctggagtgga ttgctacctg tcccgtcctg
     3901 attctgctga tctccgagat tacacaggca gaccataatg cctggggggt ggtcttctct
     3961 gattacgccc tggtggtctg cgggttcttt ggagctgtgc tgccccctta tccctgggga
     4021 aacctgttta atatcctgtc atgtgccttc tttagcttcg tggtctacag cctgtggagg
     4081 tcctttactg gggccatcaa cggagagacc ccttgcaaca ttgaagtgaa tggcctgagg
     4141 tggacacgct tctcaactgt caccacatgg accctgtttc cactgagctg gttcgccttt
     4201 acatctggca tgctgagttt cacaatgact gaggcatctt ttaccatgat cgatattggg
     4261 gccaaggtgt tcctgacact ggtgctggtc aacagcactg tggagcaggc acagaatcag
     4321 aaagtcgaag ccatcacagc tattgcagag gaactggaat ctcagatcac taactgtgac
     4381 gccattctgc agaagatgat gcctgagggg gtgctggaac agctgaagaa tggacaggca
     4441 accgaggcca aagagtacga atccgtgaca gtcttctttt ctgacatcac aaactttact
     4501 gtgattagct ccaggacctc cacaaaggac atgatggcca ccctgaacaa gctgtggctg
     4561 gagtatgatg ccatcgctaa gcggtggggc gtgtataagg tcgaaactat tggggacgct
     4621 tatctgggag tgaccggagc accagaggtg gtccccgacc acgctgatcg agcagtgaac
     4681 ttcgccctgg acatcatcga gatgatcaag acttttaaaa ctgctaccgg cgaatctatc
     4741 aacattcgca tcggcctgaa tagtggacca gtgacagccg gagtcctggg cgacctgaac
     4801 cctcattggt gcctggtggg ggataccgtc aatacagctt cccggatgga gtcaaccagc
     4861 aaggcagggc acattcatat ctccgactct acttaccaga tgatcaaggg aaaattcgtg
     4921 acccagccac tggacctgat ggaagtgaaa ggaaagggaa aaatgcagac atattgggtg
     4981 accgctagaa agtaaggtac cgctgtctca tcctactttc acctagttaa ctgcttgtct
     5041 taaaatctat gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat
     5101 ggtctcttct caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt
     5161 ttttgcgagt ttatatacct tccaattttc tttctattgt attttcaact tctaatttta
     5221 attcagggaa actgctgtac cggtagaaaa aatgctcgag atggtgagca agggcgagga
     5281 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     5341 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5401 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5461 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5521 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5581 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5641 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     5701 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5761 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     5821 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     5881 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     5941 caccggcggc atggacgagc tgtacaagta agaattccaa ctgagcgccg gtcgctacca
     6001 ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac
     6061 tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg
     6121 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     6181 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     6241 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     6301 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     6361 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     6421 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     6481 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     6541 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     6601 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     6661 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     6721 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     6781 gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct actagtcggc
     6841 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6901 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6961 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     7021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7081 aataccgcat caggcggcct taattaaggg cctcgtgata cgcctatttt tataggttaa
     7141 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7201 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7261 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7321 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7381 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7441 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7501 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7561 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7621 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7681 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7741 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7801 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7861 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7921 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7981 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8041 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8101 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8161 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8221 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8281 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8461 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8881 atttttgtga tgctcgtcag gggggcggag cctatggaaa acgccagcaa cgcggccttt
     8941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9301 cacacaggaa acagctatga ccatgattac gccaagcttg
//