LOCUS       pTH42-punc-17-YFP-CaCyc 9788 bp ds-DNA     circular     04-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..9788
                     /label="color: #ffffff"
     misc_feature    1..3184
                     /label="color: #a6acb3"
     CDS             3195..3911
                     /label="Venus YFP with monomerizing A206K mutation (Nagaiet al., 2002)"
     misc_feature    3918..5804
                     /label="color: #a6acb3"
     misc_feature    5412..5414
                     /label="color: #a6acb3"
     misc_feature    5619..5621
                     /label="color: #a6acb3"
     CDS             6070..6780
                     /label="monomeric derivative of DsRed fluorescent protein(Shaner et al., 2004)"
     CDS             8110..8970
                     /label="bla CDS"
ORIGIN
        1 ggattacacc aatcatttct cccctccgaa atgtgtagtc gtcatgacaa agtggtgaca
       61 ctggattacg gtaggctccg cccacttctt ggaaaagtga cgtgggctcg gtgagcccta
      121 aagcttttgg ttttcacaat tttctggttt ttgggatttt tgcgggaatt ttgagaggag
      181 aaagttggga gagaagatat tggaatggtc cccggggagc tgtacattta tacatctaga
      241 ctgaagctgg ggtattgcaa aaagagaaaa gcctatcact ttctttccat cctgcatact
      301 tgccaaaaag ttttggctga gcttattttt aaaattggaa ttttttggaa ttttcagaaa
      361 agaaaaagtt ttttcttttt ttttaattta aaaaattttt gctatttatt gcatggtact
      421 acagaaaaac ttgaatgaaa taaaaaattt ggaaaattca agaaaacttt ttttttcata
      481 gtttatttat tcgagtttta atgtggcttt tattttgttc ggtttttgtt ttaaaaagat
      541 tttatacaaa atttcaagaa tttacaattt ttccgttgta aaaaatccaa aaaaaaatcc
      601 aaaaaaatag ttcagacctt tttttaaatt ttaaatttaa aaattcaata ccaaaaaatt
      661 aaaaaaaatt agtttttccg agaataaagg aaaaatgttg aaaaaatatt atatataaaa
      721 tttccagaaa atgtgaaaac tccaaaaaac tgcagacttt tccccaaact agctattttt
      781 tgaattcatt acctttaaag gtggagtacc gaaatttaag actttgcttt tttagaccca
      841 aaatggtcca aaactaccaa atttcgtaat gagacgttct gaaaattttt caaaaaaatg
      901 ttatgggagc tcaaagtttt tgaaaaaatg gtatattttt agctaatttt aaccacttct
      961 tggcgtcgca gcggttggaa ctcaatttta attaaattat catccttcaa tgtccttcaa
     1021 tctttgcaat ttcaatcgac catacgcgtt cagagcattt ttttttttcg tttttcctcc
     1081 cgtccagcac atatttaaat attctctctc cccctcccct ggttcctaaa aacttgggaa
     1141 aggggaattt cgaaggaggt ggaagcttct tcttctcccc gagcacacac acacatcctc
     1201 tcttcaaaat ctttaagaaa tagtgcgtgt gaatgactag acgctgaagc taacttggtt
     1261 tccgcgaaca atcccaatca gtgcctgtgc cgaggcttcg gagaagaaga gattgacgtc
     1321 gtctttttct ttttttgtga aacagaataa aaaagaatac gaaatggact ttttcacaca
     1381 ttttcgttac ttttccagaa aaaaacgaag ctctagccat ggaagtttga attgaaaacc
     1441 taaaccccga tgtgtattca tttgagctta agcctaagcc taattctacg cctaagcata
     1501 agcctaagcc tatgttcaag cctaagccaa attgtagaac gttaccaaaa gatcaaacct
     1561 atttttccta cagaaaaggt ctaatgataa ctgttaagcg agagacaagg agagatatat
     1621 atcaaaaact agcccaaact gtgattaaca tctattaact gacctcaaaa aaaaaaatag
     1681 tagagattaa atactgctca aaaagtttct tggaaaagta aattgttagg tttctggggc
     1741 ctgtaaaact ggaccgagtt ttgaagaatt tttttaactt ttcaaagttt ggcgtcactg
     1801 aaagctttat attgtacatg ttccaccaat tttcttttgt atccgttttt tctgtctcaa
     1861 aacggagctc gagcccatga cgtgtggata ccgtgagaat ccgcccaatt ccttcacatc
     1921 gatctcatat ttctttccca gtttatattg aattcccgtt gaattgcggc tttgcagggg
     1981 agaagatgtg cgtctttata tttttttttc tatccttacc cgccgtgaat ccggctcgaa
     2041 ttgagccaat ttttatgaat agtagagcta attgacttcg tctccctaga attgatgagg
     2101 taaagctcgt ggggagatat ggagattttt cgattttttt tccagtaatt ttcagttcaa
     2161 aatttgagct tttgagcagg agttttgaga tttgtgccaa aatttcctat tattttttca
     2221 aatatttttg cttttgtaaa aaaggtgtag tagaagtttt gtagtgccac tatttttttt
     2281 ttgaaaatta tgttttggcg gaaagttctt taaaaaagtt aatcaaaatt tttggcgctt
     2341 tagtcaaatt aattttctgg caaaaatatt agtaattttg gcgtgagttc aattttccca
     2401 ttcttaaaat ttcacacaat taagaatttt aagatttggg tttttttttc aacaaaaaat
     2461 caaatattta catgaaaatt cggcgggaaa taaaaaaaaa tattccgatt caatgtgtga
     2521 ataacttttt gcggggaatt caaatttgca ttatttaaac tttttttaag ctcgaaaaat
     2581 tgaaattctt aaataaataa ataaaatttt cctcgaattt tgaatgtttc gattttttag
     2641 cgttattttt tcaaaatttc cactaaaact ctagttctaa agttctaaaa ctttaaaaac
     2701 ttcctaatgt tgtggtcttg tgtttttttg gcagcgattt ccaaatttct gaaattccaa
     2761 caaaaaaacc actacgtcag tttgcatatc caaccccccc cccccccccc ccccctctga
     2821 gccaatcaat catgtgcatt ttgaattggc gccaaggcaa tcttcgtaaa aaataacaat
     2881 agagtcatcc ctcagaacag aaaagaaacc cgcccggggg ctcaaataca catgtcattt
     2941 ttcttttttc gcctgatttt tagcctaaat tagtgtttag caatttttta aggtaactcg
     3001 gtggagtcat ttgaactttt taaactaaaa tttcagaaaa aattcttttt ttttgtcaaa
     3061 aaataatttt cccgccggaa tttttcaaaa aaataatttt tattttattt ttcaagttcc
     3121 ttgtttcctc tttatttttt tattttgccc ccaaaagttt tacactaaaa tatttaattt
     3181 tcagctcggc tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3301 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     3361 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     3421 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     3661 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     3721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     3841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3901 agctgtacaa gggatcaatg tctatgaaag ataaagataa taacctgcgg ggagcatgta
     3961 gtggctgttc ctgccctgaa tactgttact caccaacctc aaccctgtgc gacgattgca
     4021 agtgtagcgt gacaaaacac cctatcgtcg agcagccact gactcgaaac ggaagtttcc
     4081 ggagctccgg agcctcactg ctgccatctc ccagtcagcc caatatcaag gtcactggct
     4141 ctagtaccgc ctcaagcaac gctaatatgc ggaacagaca gaacaattca ctgagcgtgt
     4201 ccaatgtccg cagcacctcc tctgcaagtt caagcaacgt gtcctctccc gccaatagcc
     4261 gacctggctc tcctagtaag cagtccgctc tgcagcagta ccagacaaac atcgcagaca
     4321 tgtggtcctg ggatatgatg ctgagtaccc cttcactgaa attcctgaca ggacagttta
     4381 tcatgtgggc cattctgacc gtggccggcg ctttctacgc tctgtttatc caggaaagac
     4441 aggcttataa taggggctgg gcagacattt ggtacggcta tggggccttc ggatttggca
     4501 tcgggattgc tttcagttat atgggatttg caggcgccag aaacccagag aagaaagcac
     4561 tgtccctgtg cctgctggga gtgaacatca tcgcttttag ttcatacatc ctgattatgc
     4621 tgcggctgac tccaaccatc gaaggcaccc tgagcaaccc agtggagcct gcaagatacc
     4681 tggagtggat tgctacctgt cccgtcctga ttctgctgat ctccgagatt acacaggcag
     4741 accataatgc ctggggggtg gtcttctctg attacgccct ggtggtctgc gggttctttg
     4801 gagctgtgct gcccccttat ccctggggaa acctgtttaa tatcctgtca tgtgccttct
     4861 ttagcttcgt ggtctacagc ctgtggaggt cctttactgg ggccatcaac ggagagaccc
     4921 cttgcaacat tgaagtgaat ggcctgaggt ggacacgctt ctcaactgtc accacatgga
     4981 ccctgtttcc actgagctgg ttcgccttta catctggcat gctgagtttc acaatgactg
     5041 aggcatcttt taccatgatc gatattgggg ccaaggtgtt cctgacactg gtgctggtca
     5101 acagcactgt ggagcaggca cagaatcaga aagtcgaagc catcacagct attgcagagg
     5161 aactggaatc tcagatcact aactgtgacg ccattctgca gaagatgatg cctgaggggg
     5221 tgctggaaca gctgaagaat ggacaggcaa ccgaggccaa agagtacgaa tccgtgacag
     5281 tcttcttttc tgacatcaca aactttactg tgattagctc caggacctcc acaaaggaca
     5341 tgatggccac cctgaacaag ctgtggctgg agtatgatgc catcgctaag cggtggggcg
     5401 tgtataaggt cgaaactatt ggggacgctt atctgggagt gaccggagca ccagaggtgg
     5461 tccccgacca cgctgatcga gcagtgaact tcgccctgga catcatcgag atgatcaaga
     5521 cttttaaaac tgctaccggc gaatctatca acattcgcat cggcctgaat agtggaccag
     5581 tgacagccgg agtcctgggc gacctgaacc ctcattggtg cctggtgggg gataccgtca
     5641 atacagcttc ccggatggag tcaaccagca aggcagggca cattcatatc tccgactcta
     5701 cttaccagat gatcaaggga aaattcgtga cccagccact ggacctgatg gaagtgaaag
     5761 gaaagggaaa aatgcagaca tattgggtga ccgctagaaa gtaaggtacc gctgtctcat
     5821 cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta gtatctaaaa
     5881 ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt gtatatttat
     5941 tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt ccaattttct
     6001 ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgctgtacc ggtagaaaaa
     6061 atgctcgaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     6121 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     6181 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     6241 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     6301 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     6361 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     6421 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     6481 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     6541 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     6601 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     6661 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     6721 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     6781 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
     6841 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
     6901 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     6961 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     7021 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     7081 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     7141 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     7201 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     7261 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     7321 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     7381 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     7441 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     7501 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     7561 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     7621 ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     7681 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7741 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7801 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7861 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aattaagggc
     7921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9721 ctatggaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9781 ctcacatg
//