LOCUS       pMS04-myo3-bPGC-SL2-mCh 7812 bp ds-DNA     circular     04-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..7812
                     /label="source"
     misc_feature    1..2385
                     /label="pmyo-3"
     misc_feature    2404..3456
                     /label="bPGC"
     misc_feature    3478..3708
                     /label="SL2"
     misc_feature    3733..4443
                     /label="mCherry"
     misc_feature    4572..5299
                     /label="unc54"
     misc_feature    5773..6633
                     /label="AmpR"
     misc_feature    6788..7406
                     /label="pBR322_ori"
ORIGIN
        1 cggctataat aagttcttga ataaaataat tttcccgaca aaacatgagt atttctttcg
       61 aaaataaaag tgcaggctaa ttagagatta ttctgtaatt aactgcataa tttgtcacgt
      121 gccatagttt tacattccac tacgtcatag ttcttaaaat actaatctcc tgaaaataga
      181 agtaggtgaa gaaagtttaa ttatcagttc taaaatgaca attgatcttt ggaatatgtt
      241 ctgaaactac cgatcattga acagatgcta tttgaatgat atagaattgt atatttgcaa
      301 tttctgaaac gcgttcttaa aggcacacag attaattcaa aagggtctgg ccgcaaaaag
      361 gtttatggtg gccgattttg agttttgtgt gtgattgctt tttcacaatc agtgttttca
      421 ggattatgtg atgaactaga tcttcaagtt tcgttacatt tcatatgttt tcggaactca
      481 cgaagtacat attgggtatt gtgctcaaaa aattcagcaa tcagcttcgc tccgctgact
      541 ttagaaccca aaaaaatagt atggccaaac tgactgtgtt acgatcattt caatttttca
      601 atacatattt aagatttcta agagtaagaa ggtcaaaaac tgttctggaa tacatatata
      661 tttttcaggt tacaattagt caaaaagtgc actgaaatat acgttttaat ttcacgaata
      721 acccaattag ttcaatgtat ttttggtcaa ccaacgttaa agtttggctt ccaaccaatt
      781 atcatttctg atcaaccaca atgttttttc tttatctgca agttaatttt ttatttttat
      841 ccagatgttt ggcatatttt tcaattcttc actagcgccc acttcttgca cttccggcgc
      901 cctgaatcta atgcatctgt tgcaagaatt gaaagaccaa tcaacacatt gttttcttca
      961 cgagatactg aagaaaatga ataaaaacag agaaaaagag ccatgtgatt agtgacaact
     1021 gttgctaaca gataccatag cttggacttg gtacgtgatg gcaacgtatg ggtcaacaaa
     1081 aatgattgca gagggggtgc aaaacagtca agtcgagaaa atatgaaaaa cagaaaacaa
     1141 agaacagaaa aatgggtttg agagtcagta taatttataa aagaaaaatt gtacatagaa
     1201 attaaccatt tttgtagaag aagttatttt tcaagcatcg ttaaaaatta ttcaaagcac
     1261 cttatttcat atttaatttt aaacatggtt aaatgaacaa cacggtgcgc aatcaggaaa
     1321 acttgaaatc tgaaactgtt gttgtgatct tcttcgcaac tgttcagata gcactagtgt
     1381 aatgttaaga gtgcgcgaat ataatggaat ataatggatc acacctcctg ccatcaggta
     1441 aacgtctctg ttatcacata tttccaacta ttaaattttt accttttaca gttttacatt
     1501 tttttgaaaa aagtaacttt ttgtcttcaa aatccctgac gaaaatatca aatattttaa
     1561 tcgagactgc agaggaaccg attgatgatt tggaaaatcc agctttacct gtgtaagaac
     1621 tgaaaagttt cataacccta gggtattccc agttacattc cccactggct aacaatagca
     1681 cccagttttt catcaccttt cttcaaattt ctcggcgatt tgttaaaaac aaaatttgtg
     1741 tcccttctct gatatctcta tgtctctaaa cacaagttca tcggaaaacg aaggagggta
     1801 ggtgttggtt gggctcccga agtgaaaata gaagagcaag aatagaatat tagagagaga
     1861 gtgcagagag ggcgggatag ctcccgggat tccgttttct tcttctttat cttcaacgat
     1921 gatgtgtgtg cgtgttgtat agattctgtt gctcccccac aactcgctcc gaaggctcaa
     1981 tacaattcaa ttgatattgg aggagagcct accggagtgg gaggataaga agaaacataa
     2041 gaagaagaag aagaagaagc atgcttctgg tttttgatgc tatgaaaacg gcacaaaaag
     2101 atgattgagg tcccttttca ataccttctc tcatctttca aatcccattg aaacctaaaa
     2161 cttctcacca cgctttacca ttgttctcca aaaacttata gcaatgtcta taactttttt
     2221 atctctgaaa agcagtgttc catttttctt tttcctattt tatttcaatt gtttctcaca
     2281 tttcgtttgg attctttgct tgtcaaccag cttcttcttc cacttttacc gtctaatttt
     2341 cagggcaggg agccatcaaa cccacgacca ctagatccat ctagaggatc caagcttgcc
     2401 accatgatga agcggctggt gtacatcagc aagatcagcg gccacctgag cctggaagag
     2461 atccagcgga tcggcaaggt gtccatcaag aacaaccagc gggacaacat caccggcgtg
     2521 ctgctgtacc tgcagggcct gttcttccag atcctggaag gcgagaacga gaaggtggac
     2581 aagctgtaca agaaaatcct ggtggacgac cggcacacca acatcctgtg cctgaaaacc
     2641 gagtacgaca tcaccgaccg gatgttcccc aactgggcca tgaaaaccat caacctgaac
     2701 gagaacagcg agctgatgat ccagcccatc aagagcctgc tgcagaccat cacccagagc
     2761 caccgggtgc tggaaaagta catgcccgcc agagtgatct acctgatcaa ccagggcatc
     2821 aaccccctga ccgtggagcc ccagctggtc gagaagatca tcttcttcag cgacatcctg
     2881 gccttcagca ccctgaccga gaagctgccc gtgaacgagg tggtcatcct ggtcaaccgg
     2941 tacttcagca tctgcacccg gatcatcagc gcctacggcg gcgaagtgac cgagttcatc
     3001 ggcgactgcg tgatggccag cttcaccaaa gagcagggcg acgccgccat ccggaccagc
     3061 ctggacatca tctccgagct gaagcagctg cggcaccacg tggaggccac caaccccctg
     3121 cacctgctgt acaccggcat cggcctgagc tacggccacg tgatcgaggg caacatgggc
     3181 agcagcctga agatgaaaca cggcctgctg ggggacgccg tgaacgtggc cgccaggctg
     3241 gaagccctga caagacagct gccctacgcc ctggccttta cagccggcgt gaagaagtgc
     3301 tgccaggccc agtggacctt catcaacctg ggagcccacc aggtcaaggg caagcaggaa
     3361 gccatcgagg tctacaccgt gaatgaggcc cagaagtact acgacaccct gcagatcacc
     3421 cagctgatcc ggcagaccct ggaaaacgac aagtaactag cgtcgacggt accgctgtct
     3481 catcctactt tcacctagtt aactgcttgt cttaaaatct atgcttctct ttagtatcta
     3541 aaattttcct agaagcttac aagtatataa atggtctctt ctcaataaag gttgtatatt
     3601 tattcatctt attgaatctg ccatttcctc gtttttgcga gtttatatac cttccaattt
     3661 tctttctatt gtattttcaa cttctaattt taattcaggg aaactgctgt accggtagaa
     3721 aaaatgctcg agatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3781 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3841 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3901 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3961 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     4021 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     4081 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     4141 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     4201 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4261 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4321 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4381 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4441 taagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     4501 taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt
     4561 taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     4621 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     4681 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     4741 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     4801 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     4861 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     4921 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     4981 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     5041 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     5101 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     5161 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     5221 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     5281 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     5341 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5401 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     5461 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5521 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaattaag
     5581 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6001 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6301 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7441 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7501 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7561 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7621 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7681 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7741 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7801 acgccaagct tg
//