LOCUS       pCS2-zfTpo              4771 bp ds-DNA     circular     09-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     mRNA            1..1
                     /label="mRNA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     35..52
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             94..775
                     /label="zf Tpo"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    175..753
                     /label="zf tpo CDS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    776..796
                     /label="polylinker [Split]"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(799..816)
                     /label="T7"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    824..1015
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1065..1084)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1102..1122)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(1128..1150)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1490..2172)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2270..2929)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3390..3696)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3715..3783)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCG CCCTTGAAGT CTGAAAGCGT
      121 GGGGGCCATG CACATGATAA AGCTTTCGCG TTACTTCGAA AAAGACGCTC TCCAATGGAA
      181 AGCAACGTGA CTGGAGATTT CATGTTGTCG CTGGGCATGG ATTTAAATAG AGTGCTGGTT
      241 GTAGTTTTCA GCATGGTTGT CTCTGAACTG TCTCACGTTC ACACTAGACC AATTGACTTT
      301 GTGTGTGACA GCGAGGCCAG GAGAGTGATG AACAAGGTGA AGGATCTACA AGAAGAAAAG
      361 GTAATCTGCA GTGGCGTAGA CGCTCTCCCC TCTTCTATTC AATTACCATG CATCAGCATT
      421 CACAAAGCAA CCTGGGAGAG AAAATCAGTA CAGGAGCGCA GGGCCGAGAT CCTGCTGTCT
      481 TTGGGGACTC TAGCGCAGGA TGTTCGGTCT GCCAGGACAC TCAGCCAACC TGGCTGTGGA
      541 CTCACTCTGC TGAAGCGCTT GGAACACAGT ATCAACAACT ACCTGCATGT AGTCAGACTG
      601 CTCCACGTGG AGCAGGGGGA GCAGGAAGGG CCAGCGGTAG AGAGGTGTCT GGGTCAGCCC
      661 TCGAAGGATT TGGGTTTGGT GCTGAAGTAC TTTGGGCTTC TGCTCACAGG CAAGCTAGAG
      721 TTGCTCATTG CAGAGATGGC AAAATGGTGC TAGGGATCCC GCCAAGGGCG AATTCAAGGC
      781 CTCTCGAGCC TCTAGAACTA TAGTGAGTCG TATTACGTAG ATCCAGACAT GATAAGATAC
      841 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA
      901 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC
      961 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAATTC
     1021 GCGGCCGCGG CGCCAATGCA TTGGGCCCGG TACCCAGCTT TTGTTCCCTT TAGTGAGGGT
     1081 TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
     1141 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
     1201 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
     1261 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
     1321 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
     1381 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
     1441 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
     1501 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     1561 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     1621 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     1681 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     1741 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     1801 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     1861 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     1921 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     1981 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     2041 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     2101 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
     2161 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
     2221 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
     2281 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
     2341 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
     2401 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
     2461 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
     2521 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
     2581 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
     2641 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
     2701 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
     2761 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
     2821 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
     2881 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
     2941 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
     3001 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
     3061 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
     3121 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
     3181 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
     3241 CCCGAAAAGT GCCACCTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT
     3301 TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC
     3361 AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT
     3421 AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT
     3481 ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG
     3541 GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG
     3601 AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC
     3661 GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC
     3721 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG
     3781 CCAGTCGACC ATAGCCAATT CAATATGGCG TATATGGACT CATGCCAATT CAATATGGTG
     3841 GATCTGGACC TGTGCCAATT CAATATGGCG TATATGGACT CGTGCCAATT CAATATGGTG
     3901 GATCTGGACC CCAGCCAATT CAATATGGCG GACTTGGCAC CATGCCAATT CAATATGGCG
     3961 GACTTGGCAC TGTGCCAACT GGGGAGGGGT CTACTTGGCA CGGTGCCAAG TTTGAGGAGG
     4021 GGTCTTGGCC CTGTGCCAAG TCCGCCATAT TGAATTGGCA TGGTGCCAAT AATGGCGGCC
     4081 ATATTGGCTA TATGCCAGGA TCAATATATA GGCAATATCC AATATGGCCC TATGCCAATA
     4141 TGGCTATTGG CCAGGTTCAA TACTATGTAT TGGCCCTATG CCATATAGTA TTCCATATAT
     4201 GGGTTTTCCT ATTGACGTAG ATAGCCCCTC CCAATGGGCG GTCCCATATA CCATATATGG
     4261 GGCTTCCTAA TACCGCCCAT AGCCACTCCC CCATTGACGT CAATGGTCTC TATATATGGT
     4321 CTTTCCTATT GACGTCATAT GGGCGGTCCT ATTGACGTAT ATGGCGCCTC CCCCATTGAC
     4381 GTCAATTACG GTAAATGGCC CGCCTGGCTC AATGCCCATT GACGTCAATA GGACCACCCA
     4441 CCATTGACGT CAATGGGATG GCTCATTGCC CATTCATATC CGTTCTCACG CCCCCTATTG
     4501 ACGTCAATGA CGGTAAATGG CCCACTTGGC AGTACATCAA TATCTATTAA TAGTAACTTG
     4561 GCAAGTACAT TACTATTGGA AGGACGCCAG GGTACATTGG CAGTACTCCC ATTGACGTCA
     4621 ATGGCGGTAA ATGGCCCGCG ATGGCTGCCA AGTACATCCC CATTGACGTC AATGGGGAGG
     4681 GGCAATGACG CAAATGGGCG TTCCATTGAC GTAAATGGGC GGTAGGCGTG CCTAATGGGA
     4741 GGTCTATATA AGCAATGCTC GTTTAGGGAA C
//