LOCUS       pCS2-Kitlga_1+2FINAL    5018 bp ds-DNA     circular     08-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     35..52
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             79..1007
                     /label="Kitlga 1+2"
                     /ApEinfo_revcolor=#4444ff
                     /ApEinfo_fwdcolor=#4444ff
     misc_feature    85..104
                     /label="kitla1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    122..193
                     /label="SP"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             122..940
                     /label="CDS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             122..940
                     /label="Translation 122-940"
     primer_bind     194..213
                     /label="kitla3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    194..739
                     /label="NC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(650..669)
                     /label="kitla5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    739..793
                     /label="TM"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    794..940
                     /label="C"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     921..940
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(982..1001)
                     /label="kitla2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1046..1063)
                     /label="T7"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    1071..1262
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1312..1331)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1349..1369)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(1375..1397)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1737..2419)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2517..3176)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3637..3943)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3962..4030)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCGGTTTC GCTGACATTG GAGTTACTCC TCTCGTTCCA
      121 TATGAAGAAG TCAAACATTT GGATATGCAC CTGTGTCCAT TTATTGCTGT ACATCACAGT
      181 TGCTGCCTAT TCCATTGAAA TAGGAAATCC CATTACAGAT GACATCAAGA AGATTTCTTT
      241 GTTGAAACAG AATATTCCAA AAGACTACAA GATTACAGTG CGTTATATAC CTAAAGAAGT
      301 GAGTGGCATG TGCTGGGTGA AGCTAAATGT ATTTCACTTG GAGGTGAGCT TAAAAGGCCT
      361 GGCGCAGAAG TTTGGGAACA TATCCTCCAA TAAAGATAAT ATAGGCACTT TCGTCCAAAT
      421 ACTGCAAGAT ATGCGGTATC ACATTGGACC TGGTTTGGAG GATTCAATGC TTGACTTTGA
      481 GTGTCACTAT GTAGAGGAGA TGTGGCTTAC GGCGAAGTAT TTTGAATTTT TGGAGGATTT
      541 TTTCAACACT GCCAATTCAT CAAGAGATGC TGAGGACTGC GAGCCACCGC CATGTCCCAC
      601 ATCCACAAAA ACAACAATAA CAACAACTAC AACAGCGTCG ACAACATCAG CGCAACATAG
      661 TACAAATGAG AAACGAAACG GTTTGCCTGA TGACCCCGAA AAAGGGGCAT TTCTTTCAAA
      721 AGTTTTGGAG AGTAATCTCA TGTGGCTCCT CACCATCCCG TTTGCAATCG CAGTGGTGGT
      781 TTTGCTGGTC TGGAAGATTA AGTCCAGAAG AAACACCCCC CAAACTGACC GGAGTCCAGA
      841 AGAAGGTCCC GCTCTGTTCT CAGGAGAGGA AGCCAATATA TCGCCATTAG ATGTGGGGAT
      901 ATCTGAAAAA AACAGATTAA ATATTATCAT GGATGTGTAA ATCCAGCCCC AGCTTAAGCA
      961 ACGCAGTCAC TCCTTCACAT GGCCCAACCT ACAGGAACAA AGGATCCCAT CGATTCGAAT
     1021 TCAAGGCCTC TCGAGCCTCT AGAACTATAG TGAGTCGTAT TACGTAGATC CAGACATGAT
     1081 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
     1141 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
     1201 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
     1261 TTAATTCGCG GCCGCGGCGC CAATGCATTG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG
     1321 TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
     1381 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
     1441 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     1501 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     1561 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     1621 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     1681 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     1741 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     1801 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     1861 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     1921 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     1981 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     2041 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     2101 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     2161 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     2221 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     2281 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     2341 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2401 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2461 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2521 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2581 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2641 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2701 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2761 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2821 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2881 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2941 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     3001 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     3061 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     3121 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3181 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3241 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3301 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3361 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3421 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3481 ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT
     3541 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
     3601 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
     3661 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG
     3721 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
     3781 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
     3841 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
     3901 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
     3961 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
     4021 TATTACGCCA GTCGACCATA GCCAATTCAA TATGGCGTAT ATGGACTCAT GCCAATTCAA
     4081 TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT ATGGACTCGT GCCAATTCAA
     4141 TATGGTGGAT CTGGACCCCA GCCAATTCAA TATGGCGGAC TTGGCACCAT GCCAATTCAA
     4201 TATGGCGGAC TTGGCACTGT GCCAACTGGG GAGGGGTCTA CTTGGCACGG TGCCAAGTTT
     4261 GAGGAGGGGT CTTGGCCCTG TGCCAAGTCC GCCATATTGA ATTGGCATGG TGCCAATAAT
     4321 GGCGGCCATA TTGGCTATAT GCCAGGATCA ATATATAGGC AATATCCAAT ATGGCCCTAT
     4381 GCCAATATGG CTATTGGCCA GGTTCAATAC TATGTATTGG CCCTATGCCA TATAGTATTC
     4441 CATATATGGG TTTTCCTATT GACGTAGATA GCCCCTCCCA ATGGGCGGTC CCATATACCA
     4501 TATATGGGGC TTCCTAATAC CGCCCATAGC CACTCCCCCA TTGACGTCAA TGGTCTCTAT
     4561 ATATGGTCTT TCCTATTGAC GTCATATGGG CGGTCCTATT GACGTATATG GCGCCTCCCC
     4621 CATTGACGTC AATTACGGTA AATGGCCCGC CTGGCTCAAT GCCCATTGAC GTCAATAGGA
     4681 CCACCCACCA TTGACGTCAA TGGGATGGCT CATTGCCCAT TCATATCCGT TCTCACGCCC
     4741 CCTATTGACG TCAATGACGG TAAATGGCCC ACTTGGCAGT ACATCAATAT CTATTAATAG
     4801 TAACTTGGCA AGTACATTAC TATTGGAAGG ACGCCAGGGT ACATTGGCAG TACTCCCATT
     4861 GACGTCAATG GCGGTAAATG GCCCGCGATG GCTGCCAAGT ACATCCCCAT TGACGTCAAT
     4921 GGGGAGGGGC AATGACGCAA ATGGGCGTTC CATTGACGTA AATGGGCGGT AGGCGTGCCT
     4981 AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAAC
//