LOCUS       pCS2-Kitlgb_1+2FINAL    5016 bp ds-DNA     circular     08-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     35..52
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    79..104
                     /label="kitlb1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             79..1005
                     /label="CDS"
                     /ApEinfo_revcolor=#4444ff
                     /ApEinfo_fwdcolor=#4444ff
     CDS             117..209
                     /label="SP"
                     /ApEinfo_revcolor=#4444ff
                     /ApEinfo_fwdcolor=#4444ff
     CDS             117..920
                     /label="Translation 117-920"
     misc_feature    150..670
                     /label="NC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    207..226
                     /label="kitlb3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(657..677)
                     /label="kitlb5"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    671..743
                     /label="TM"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    744..920
                     /label="C"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(980..1005)
                     /label="kitlb2"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1044..1061)
                     /label="T7"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    1069..1260
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1310..1329)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1347..1367)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(1373..1395)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1735..2417)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2515..3174)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3635..3941)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3960..4028)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCGGAGAC ACGGCTGATT TGTTTTTAAT ATTCCCATGT
      121 TCCACATGAG GGAGGTTAAG ATTGGAGAAA GCATTTGTGT ATTAGTGTTG CTTTTTTCGG
      181 GGCTTGTGAC CTGCTCAGGT GTTTTTGGGA GCCCTTTAAC AGATGATGTG GCCACGTTAG
      241 ATACCCTGAG TGAGAATATT CCCAGTGACT ACAGAATACC CATCAAGTTC ATCACAAAGG
      301 ATGTGGGTGG AGCTTGCTGG CTGCATTTGA ACCTGTATCC AGTCGAGAGC AGTTTAAAAA
      361 AATTGGCAGT CAAGTTTGGC AACCAGTCCA CCAATAAGGC CAACATCACC ATTTTCATAA
      421 CAATGCTGCA GGATTTTCGT TTCACTCTGA ACAGTGACGA CCTGGAGGAC AGAATGCAAG
      481 CCTTCAAGTG CCACTACAGA AGGGAAAAGT GGCCGACTAG GCGCTTCTTT AGCTATGTTA
      541 AATCCGTTCT TACAGTGGCC GGCTCCACAT ATGGTGATAT TCCTCCATGC ACACCTCCTC
      601 CTTGCCAGAC CCTTGCAGCT CCTCCTTTCA CGCCAGGTCA AAGCAGACAG CAGAATGGCA
      661 TGAACTCTGC GGTTCATGGG CTCCTGGCGC TGCTCATCAT CCCCAGTGTG GCTATTCTAG
      721 TTCTCACTAT CCAAATGGCA TTAGGAAGAA GAGGTCGTTG TGGAGCAAGA ATGCGTGAAA
      781 TTGAGCCTCA CGACAGAGCT GAAGAAAACA GAAATGAGCT CCACAGCGGA GCTGCACAGG
      841 AGGACCCGGC TTCCACCTCC GCCAGTGAAC AAGACAGGGC GTGGCTGGAT TCTTTAGGGT
      901 GTGCAGACAC AGAGGTCTAA TAAAAAAAAA AAGCAAAAGG AAAGTCGACG GAAACGCCAG
      961 TATGCTCCGG AGTTATGAAG GGAATATCCA GAACGGGATG GATCCCATCG ATTCGAATTC
     1021 AAGGCCTCTC GAGCCTCTAG AACTATAGTG AGTCGTATTA CGTAGATCCA GACATGATAA
     1081 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
     1141 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
     1201 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT
     1261 AATTCGCGGC CGCGGCGCCA ATGCATTGGG CCCGGTACCC AGCTTTTGTT CCCTTTAGTG
     1321 AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     1381 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
     1441 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
     1501 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
     1561 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
     1621 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     1681 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     1741 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1801 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1861 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1921 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1981 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2041 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2101 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2161 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
     2221 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2281 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2341 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     2401 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     2461 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     2521 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     2581 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     2641 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     2701 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2761 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2821 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2881 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2941 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     3001 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     3061 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     3121 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     3181 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     3241 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     3301 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     3361 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     3421 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     3481 ATTTCCCCGA AAAGTGCCAC CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA
     3541 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
     3601 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA
     3661 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     3721 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     3781 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     3841 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     3901 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC
     3961 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA
     4021 TTACGCCAGT CGACCATAGC CAATTCAATA TGGCGTATAT GGACTCATGC CAATTCAATA
     4081 TGGTGGATCT GGACCTGTGC CAATTCAATA TGGCGTATAT GGACTCGTGC CAATTCAATA
     4141 TGGTGGATCT GGACCCCAGC CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA
     4201 TGGCGGACTT GGCACTGTGC CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA
     4261 GGAGGGGTCT TGGCCCTGTG CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG
     4321 CGGCCATATT GGCTATATGC CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC
     4381 CAATATGGCT ATTGGCCAGG TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA
     4441 TATATGGGTT TTCCTATTGA CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA
     4501 TATGGGGCTT CCTAATACCG CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT
     4561 ATGGTCTTTC CTATTGACGT CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA
     4621 TTGACGTCAA TTACGGTAAA TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC
     4681 ACCCACCATT GACGTCAATG GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC
     4741 TATTGACGTC AATGACGGTA AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA
     4801 ACTTGGCAAG TACATTACTA TTGGAAGGAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA
     4861 CGTCAATGGC GGTAAATGGC CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG
     4921 GGAGGGGCAA TGACGCAAAT GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA
     4981 TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAAC
//