LOCUS       pCS2+GCSF10+7           4790 bp ds-DNA     circular     09-JUN-2021
DEFINITION  .
KEYWORDS    "Created At:2007-02-11" "Locus:                        4095 bp     
            ds-DNA     circular SYN 11-FEB-2007"
FEATURES             Location/Qualifiers
     ORF             1..1
                     /label="ORF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     35..52
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(95..108)
                     /label="ß-galactosidase alfa-fragment (lacZ’) [Split]"
                     /ApEinfo_revcolor=#339900
                     /ApEinfo_fwdcolor=#339900
     gene            109..794
                     /label="GCSF10+7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    114..785
                     /label="zfcsf3a"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(795..810)
                     /label="ß-galactosidase alfa-fragment (lacZ’) [Split]"
                     /ApEinfo_revcolor=#339900
                     /ApEinfo_fwdcolor=#339900
     misc_feature    complement(818..835)
                     /label="T7"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    843..1034
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1084..1103)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1121..1141)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(1147..1169)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1509..2191)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2289..2948)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3409..3715)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3734..3802)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCC TGCAGCCCTG AACATGGGGC
      121 TTTGTTACTA CTGGAGTCTG GTGATTGTGC TCCTCTGCTT TTTGATTGGT GTTGCTATAA
      181 TGAACTTCCA GGCTCAGTTT TTTTTCACAT TAGTGGGGAT CGTTGTGTCT GCTCCTGTTC
      241 CTCAGAAACT GGACATCATG AACAAGGATA CTATTGAACA AGCACACAGC TTAATCAGCA
      301 AAACTCTGGA AGATATTCCT GCAACTCATG CAGCATGGGT AAAGAGCAAG AGTCTGGCTT
      361 GGGGCAGCAG CACAGATAAG CTGCAGCACT TGAAACATTA CATACCCTCA GCCCCTGTTC
      421 TCCAGAACAT CACTGATATC AGCAGTCTGG AAACCTGTCT TGATAAAATA GTGAGGGGCC
      481 TGCAACTACA TCTGAACCTC CTGAAAGATC TCATTGAAGC AACGACCCTG TCGCAGACTG
      541 ACCAGGTGAC AGAACTGCAG GCTGACATTC AAGAACTTGT GCTTCTTATC GAGGAGCTTC
      601 AGAATCAGTC AGGATTTAAC CCATCACAGC AGACATCAGA AGAGCAGTCC CAGAGCTTCA
      661 AGCTCAATCT GACCCAACAC CTGAAAAGTG ATTTCCAGAC TGAGGCCGCG GCTCACCTAA
      721 TCCTGCATCA ACTGCGGGAT TTCAGCTGTG ACATCCTTCA GAGGATCCTC AGTATTCGTG
      781 TATGAATCGA TCCGGGGGGA TCCACTAGTT CTAGAACTAT AGTGAGTCGT ATTACGTAGA
      841 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
      901 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
      961 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT
     1021 GTGGGAGGTT TTTTAATTCG CGGCCGCGGC GCCAATGCAT TGGGCCCGGT ACCCAGCTTT
     1081 TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
     1141 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
     1201 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
     1261 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     1321 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     1381 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     1441 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     1501 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     1561 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     1621 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     1681 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     1741 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     1801 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     1861 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     1921 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
     1981 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     2041 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     2101 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     2161 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     2221 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     2281 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     2341 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     2401 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     2461 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     2521 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2581 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2641 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     2701 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2761 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     2821 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     2881 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     2941 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     3001 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     3061 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     3121 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     3181 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     3241 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATATTTTGT
     3301 TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG
     3361 GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT
     3421 GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT
     3481 ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT
     3541 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA
     3601 AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC
     3661 TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC
     3721 TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC
     3781 GGGCCTCTTC GCTATTACGC CAGTCGACCA TAGCCAATTC AATATGGCGT ATATGGACTC
     3841 ATGCCAATTC AATATGGTGG ATCTGGACCT GTGCCAATTC AATATGGCGT ATATGGACTC
     3901 GTGCCAATTC AATATGGTGG ATCTGGACCC CAGCCAATTC AATATGGCGG ACTTGGCACC
     3961 ATGCCAATTC AATATGGCGG ACTTGGCACT GTGCCAACTG GGGAGGGGTC TACTTGGCAC
     4021 GGTGCCAAGT TTGAGGAGGG GTCTTGGCCC TGTGCCAAGT CCGCCATATT GAATTGGCAT
     4081 GGTGCCAATA ATGGCGGCCA TATTGGCTAT ATGCCAGGAT CAATATATAG GCAATATCCA
     4141 ATATGGCCCT ATGCCAATAT GGCTATTGGC CAGGTTCAAT ACTATGTATT GGCCCTATGC
     4201 CATATAGTAT TCCATATATG GGTTTTCCTA TTGACGTAGA TAGCCCCTCC CAATGGGCGG
     4261 TCCCATATAC CATATATGGG GCTTCCTAAT ACCGCCCATA GCCACTCCCC CATTGACGTC
     4321 AATGGTCTCT ATATATGGTC TTTCCTATTG ACGTCATATG GGCGGTCCTA TTGACGTATA
     4381 TGGCGCCTCC CCCATTGACG TCAATTACGG TAAATGGCCC GCCTGGCTCA ATGCCCATTG
     4441 ACGTCAATAG GACCACCCAC CATTGACGTC AATGGGATGG CTCATTGCCC ATTCATATCC
     4501 GTTCTCACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCACTTGGCA GTACATCAAT
     4561 ATCTATTAAT AGTAACTTGG CAAGTACATT ACTATTGGAA GGACGCCAGG GTACATTGGC
     4621 AGTACTCCCA TTGACGTCAA TGGCGGTAAA TGGCCCGCGA TGGCTGCCAA GTACATCCCC
     4681 ATTGACGTCA ATGGGGAGGG GCAATGACGC AAATGGGCGT TCCATTGACG TAAATGGGCG
     4741 GTAGGCGTGC CTAATGGGAG GTCTATATAA GCAATGCTCG TTTAGGGAAC
//