LOCUS       Exported                8313 bp ds-DNA     circular SYN 05-MAR-2021
DEFINITION  pSP014.
ACCESSION   AY163491.2
VERSION     .
KEYWORDS    pSP014
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Patel SK, Dotson J, Allen KP, Fleckenstein JM.
  TITLE     Identification and molecular characterization of EatA, an 
            autotransporter protein of enterotoxigenic Escherichia coli.
  JOURNAL   Infect. Immun. 2004 Mar;72:1786-94
  PUBMED    14977988
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   fleckenstein lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 5, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/iai.72.3.1786-1794.2004"; journalName: "Infect. Immun."; 
            date: "2004-03"; volume: "72"; pages: "1786-94"
COMMENT     cloned from ETEC strain H10407 into pBAD-TOPO
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     CDS             1..4092
                     /codon_start=1
                     /label=eatA
                     /note="eatA cds"
                     /translation="MNKVFSLKYSFLAKGFIAVSELARRVSVKGKLKSASSIIISPITI
                     AIVSYAPPSLAATVNADISYQTFRDFAENKGAFIVGASNINIYDKNGVLVGVLDKAPMP
                     DFSSATMNTGTLPPGDHTLYSPQYVVTAKHVNGSDIMSFGHIQNNYTVVGENNHNSLDI
                     KIRRLNKIVTEVAPAEISSVGAVNGAYQEGGRFKAFYRLGGGLQYIKDKNGNLTPVYTN
                     GGFLTGGTISALSLYNNGQMITAPTGDIFNPANGPLANYLNKGDSGSPLFAYDSLDKKW
                     VLVGVLSSGSEHGNNWVVTTQDFLHQQPKHDFDKTISYDSEKGSLQWRYNKNSGVGTLS
                     QESVVWDMHGKKGGDLNAGKNLQFTGNNGEIILHDSIDQGAGYLQFFDNYTVTSLTDQT
                     WTGGGIITEKGVNVLWQVNGVNDDNLHKVGEGTLTVNGKGVNNGGLKVGDGTVILNQRP
                     DDNGHKQAFSSINISSGRATVILSDANQVNPDKISWGYRGGTLDLNGNNVNFTRLQAAD
                     YGAIVSNNNKNKSELTLKLQTLNENDISVDVKTYEVFGGHGSPGDLYYVPASNTYFILK
                     SKAYGPFFSDLDNTNVWQNVGHDRDKAIQIVKQQKIGESSQPYMFHGQLNGYMDVNIHP
                     LSGKDVLTLDGSVNLPEGVITKKSGTLIFQGHPVIHAGMTTSAGQSDWENRQFTMDKLR
                     LDAATFHLSRNAHMQGDISAANGSTVILGSSRVFTDKNDGTGNAVSSVEGSSIATTAGD
                     QSYYSGNVLLENHSSLEVRENFTGGIEAYDSSVSVTSQNAIFDHVGSFVNSSLLLEKGA
                     KLTAQSGIFTNNTMKIKENASLTLTGIPSVGKPGYYSPVTSTTEGIHLGERASLSVKNM
                     GYLSSNITAENSAAIINLGDSNATIGKTDSPLFSTLMRGYNAVLQGNIMGPQSSVNMNN
                     ALWHSDRNSELKELKANDSQIELGVRGHFAKLRVKELIASNSVFLVHANNSQADQLNVT
                     DKLQGSNNTILVDFFNKAANGTNVTLITAPKGSDENTFKAGTQQIGFSNITPEIRTENT
                     DTATQWVLTGYQSVADARASKIATDFMDSGYKSFLTEVNNLNKRMGDLRDSQGDAGGWA
                     RIMNGTGSGESGYRDNYTHVQIGADRKHELNGIDLFTGALLTYTDNNASSQAFSGKTKS
                     LGGGVYASGLFESGAYFDLIGKYLHHDNRYTLNFASLGERSYTSHSLYAGAEIGYRYHM
                     SENTWVEPQMELVYGSVSGKSFNWKDQGMQLSMKDKDYHPLIGRTGVDVGRAFSGDTWK
                     VTVRAGLGYQFDLLANGETVLQDASGKKHFKGEKDSRMLMNVGTNVEVKDNMRFGLELE
                     KSAFGRYNIDNSINANFRYYF"
     misc_feature    complement(1)
                     /label=stop2
     CDS             400..402
                     /codon_start=1
                     /product="serine protease catalytic triad H134, D162, S267 
                     "
                     /label=H134
                     /translation="H"
     CDS             484..486
                     /codon_start=1
                     /product="serine protease catalytic triad H134, D162, S267 
                     "
                     /label=D162
                     /translation="D"
     CDS             793..810
                     /label=serine protease motif
                     /note="serine protease motif containing Ser267"
     CDS             799..801
                     /codon_start=1
                     /product="serine protease catalytic triad H134, D162, S267 
                     "
                     /label=S267
                     /translation="S"
     CDS             4093..4095
                     /label=stop
     misc_feature    4189..4230
                     /label=V5
                     
                     /note="V5  epitope"
     misc_feature    4240..4257
                     /label=6H
                     /note="polyhistidine region"
     CDS             4800..5660
                     /label=ßlac
                     /note="ampicilllin R"
     misc_feature    5805..6478
                     /label=pBR322 ori
                     /note="origin of replication"
     CDS             complement(7009..7887)
                     /label=araC
     misc_feature    7918..8190
                     /label=Pbad
                     /note="Arabinose promoter and regulatory elements"
     misc_feature    8242..8245
                     /label=RBS
                     /note="ribosome binding site"
     misc_feature    complement(8311)
                     /label=stop1
ORIGIN
        1 atgaataaag tgttctctct taagtatagt tttttagcca aaggttttat tgctgtttct
       61 gaacttgccc gtcgcgtttc tgttaaaggg aaactgaaga gtgcttcatc aataattatt
      121 tcaccaataa caattgctat tgtttcttat gcacccccat ctcttgctgc aacagttaat
      181 gcagatatat cgtatcaaac atttcgggat tttgccgaaa ataaaggagc ttttatagtt
      241 ggcgcatcaa atataaatat ctacgataag aatggagtgt tagttggagt gcttgataaa
      301 gctccaatgc ctgattttag tagcgccacg atgaatacag ggacattacc accaggagac
      361 catacactgt actcacctca atatgttgtc acagcaaagc atgttaatgg atcagatata
      421 atgagttttg gacatattca aaataattat actgtagtag gagagaacaa ccataatagc
      481 cttgatatta aaatacggcg tttaaataag attgtcacgg aggtcgcccc tgcagaaatc
      541 tccagtgttg gagctgtaaa tggcgcttat caagaagggg ggcgttttaa agccttttat
      601 aggcttggag gtggattgca atatataaag gataaaaatg ggaatcttac accggtatat
      661 acaaatggtg gtttcctaac cggaggtact atcagtgctt taagcttata taacaatggc
      721 caaatgatta ccgcacctac aggcgatata tttaatccag ctaatgggcc tcttgcaaac
      781 tatctaaata aaggtgatag tggctctcct ttatttgcgt atgactctct ggacaaaaaa
      841 tgggttttag ttggcgttct gtcatcagga agcgagcatg gtaataactg ggttgtcaca
      901 actcaagatt ttcttcatca gcaaccaaaa catgattttg ataaaacaat atcatatgac
      961 tctgaaaagg gtagcttaca atggagatat aataaaaatt caggagtggg aacattaagt
     1021 caagagagcg ttgtgtggga catgcatgga aaaaaagggg gagatctaaa cgcaggtaaa
     1081 aatcttcaat ttacaggaaa taatggagag attattttac acgactctat agatcaaggg
     1141 gctggctatt tgcagttttt tgacaactac acagttacat ccttaactga ccaaacatgg
     1201 accggaggtg gtatcattac tgaaaaaggt gtaaatgtgc tttggcaggt taatggtgtt
     1261 aatgatgata acttacataa agttggtgaa ggcacattaa ctgttaatgg aaaaggggtt
     1321 aataatggag gactgaaagt cggtgatgga accgtaattc tgaatcaacg ccctgatgat
     1381 aatggacaca agcaagcctt tagctctatt aacatttcca gtggtcgtgc aacagttata
     1441 ctttcagatg ctaatcaagt taacccagat aaaatatcat ggggatatag aggcggtact
     1501 cttgatttaa atggaaataa tgtaaacttt actcgtcttc aggctgcaga ttatggtgct
     1561 attgtttcta acaataacaa aaacaaatct gaattaacac ttaaattaca aacactaaat
     1621 gaaaatgaca ttagtgttga tgtgaagaca tatgaagttt ttggggggca tggtagtcca
     1681 ggtgacttat attatgttcc tgcatcaaat acttacttta tcctgaaatc aaaggcgtac
     1741 ggtccatttt tcagtgattt agataatacc aatgtctggc aaaatgttgg tcacgatcgt
     1801 gataaagcga ttcaaatcgt gaaacagcag aagattgggg aaagctctca accttatatg
     1861 tttcatggac aacttaatgg ttatatggat gtaaatatac atccactctc tggtaaggat
     1921 gtgctgactc ttgatggttc tgttaatctg cctgaagggg tgataacgaa aaagtcaggt
     1981 actctgatat ttcaagggca tccggtgatt catgctggaa tgacaacctc agccggccag
     2041 agtgattggg aaaatcgtca gtttacaatg gataaactga ggcttgatgc agcaacattc
     2101 catctctcca gaaatgctca tatgcaggga gatattagtg ctgccaacgg aagcaccgtc
     2161 attctgggaa gttctcgggt ctttactgac aagaatgacg gaaccggtaa tgcggtatct
     2221 tctgttgaag ggagttccat tgcaacaaca gctggtgacc aaagttatta cagcggtaat
     2281 gtgctgctgg aaaaccattc gtctctagag gtcagggaga attttactgg tggtattgag
     2341 gcttatgaca gttctgttag tgtgacctct cagaatgcta tttttgacca tgttggtagc
     2401 tttgttaata gtagtctgct tctcgaaaaa ggagcaaaac tgacagcaca gagtggtatt
     2461 ttcacaaata acactatgaa aataaaagaa aacgcctccc tgactctgac agggatacct
     2521 tctgtaggaa agccagggta ttattcacct gtgacctcga ctactgaagg aattcatctc
     2581 ggtgagcgag ccagcctttc agtgaaaaat atgggctatc tgagttcaaa tattacagca
     2641 gagaactctg cagcaattat taatctggga gacagtaatg caactatcgg gaagacggac
     2701 tctccattat tcagtacctt aatgagggga tataatgctg ttttgcaggg caatattatg
     2761 gggccccaga gctcagtgaa tatgaacaat gctctgtggc actctgatag aaattcggaa
     2821 ctcaaagagc tgaaagccaa cgactcccaa atagagttgg gtgtaagagg gcattttgca
     2881 aaactgcggg taaaagagct tattgcgtct aactcagtgt ttcttgtaca tgcaaacaat
     2941 agccaggctg accagttgaa cgttaccgac aaactgcagg gcagcaacaa tactattctt
     3001 gttgactttt ttaacaaagc agccaatggt acaaatgtga cgttaattac tgcaccaaaa
     3061 ggcagtgatg aaaatacatt caaagccgga acccagcaga ttggattcag taatatcacg
     3121 ccagaaatca ggacagaaaa tacggataca gccacacagt gggtgctgac tggatatcag
     3181 tctgtcgctg atgccagagc ctcgaaaatc gcaacggact ttatggattc aggttataaa
     3241 tctttcctga cggaagtcaa taatctgaac aaacgtatgg gagatttacg ggatagtcag
     3301 ggagatgctg gagggtgggc gcgtatcatg aatggtaccg gttcaggtga gagtggttac
     3361 agagataact atacccacgt tcagattggt gcagacagaa agcatgagct gaacggtata
     3421 gatttattca ccggtgcatt actgacttat acagacaaca atgctagcag ccaggctttc
     3481 agcggtaaaa caaaatcgct agggggaggg gtgtatgcat caggtctctt tgagtctgga
     3541 gcttattttg acctgattgg taaatatctc catcatgata atcggtatac gttgaatttt
     3601 gcctccttgg gggaaagaag ctacacctcc cattctttgt atgctggagc tgaaatcggg
     3661 tatcgttatc acatgtcaga aaatacatgg gtggaaccac agatggaact ggtttatggt
     3721 tcggtatcag gaaagtcatt taactggaaa gaccagggaa tgcaactgag tatgaaagac
     3781 aaagactatc acccactaat tggtcgaaca ggtgtggatg taggtagagc gttctctgga
     3841 gatacctgga aagtaacagt acgtgcagga ctgggttacc agttcgattt gctggcaaat
     3901 ggagaaactg ttttacagga tgcttctggt aaaaaacact tcaaaggtga aaaagacagc
     3961 aggatgctaa tgaacgtggg gacgaatgtg gaagttaaag acaatatgcg ttttggtctg
     4021 gagttggaga agtcggcgtt tgggagatat aacatagaca actctataaa tgctaacttc
     4081 cgttattatt tctgattttg atagctgcgc agctatccaa cataatgcga actagttaag
     4141 ggagctgata tcaccttaac gatagtacag cgacaagggc gagcttgaag gtaagcctat
     4201 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     4261 agtttaaacg gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata
     4321 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     4381 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     4441 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     4501 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     4561 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
     4621 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     4681 tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg
     4741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5041 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     6661 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     6721 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     6781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc
     6901 gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     6961 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     7021 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     7081 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     7141 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     7201 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     7261 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     7321 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     7381 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     7441 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     7501 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     7561 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     7621 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     7681 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     7741 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     7801 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     7861 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     7921 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     7981 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     8041 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     8101 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     8161 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagaa
     8221 ataattttgt ttaactttaa gaaggagata tacataccca tgggctctgg atccggtgat
     8281 gacgatgaca agctcgccct tgaggaataa taa
//
