LOCUS       lyzC-GFP-pa-U6CA        9271 bp DNA     circular SYN 03-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1061..1081
                     /label=M13-rev
     misc_recomb     complement(1097..1117)
                     /label=attB4
     misc_feature    1145..3513
                     /label=lyzC promoter
     primer_bind     3563..3580
                     /label=M13-fwd
     primer_bind     3590..3609
                     /label=T7
     CDS             3678..4391
                     /label=EGFP
     primer_bind     complement(4672..4691)
                     /label=T3
     polyA_signal    4794..4985
                     /label=SV40 late polyA
     misc_feature    5195..5214
                     /label=rac guide
     misc_feature    5736..5755
                     /label=rac guide
     polyA_signal    6232..6423
                     /label=SV40 late polyA
     primer_bind     complement(7085..7104)
                     /label=T3
     primer_bind     complement(7122..7142)
                     /label=M13-rev
     misc_binding    complement(7148..7170)
                     /label=LacO
     promoter        complement(7175..7204)
                     /label=lac
     rep_origin      complement(7510..8138)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8290..8949)
                     /label=AmpR
     promoter        complement(9189..9217)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tattgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgcttttta attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttttata
     1741 tcagtgtttt agattgaagt acaatattta taggtaaaat tcaccagcat atgcttaaag
     1801 atagccatgc tccaggtcct tgtgtccttc taaggtctgt ttgtggttta agctgtttgt
     1861 tgacacacgc ttgtcttctc catttttctg tgcagttatt gtcagtttat tgcacactgg
     1921 gttgcattaa gctgcagttt tttcacaaca gttttccaca ttgtaacagt cagtctccaa
     1981 attattccat cagtcctcaa atttagtcat ttgaggccat gaaaacactt aaaactacat
     2041 aaaggtagtt tggcattagg ttttagactt gttcttgttt ggcaatattg caacctgtgt
     2101 aaaataattt gattcttttt tttttttttt tttttttaat tttcagaaag aaatggtcca
     2161 aaaggagcta tgtgaacatt tatccatgtg tttaatgttt ttttttttat tattaaacca
     2221 cttctgactt aacttttttt agttattcta ttttgtgacg agtctgaatg gccaaagtac
     2281 ttttgaatcc aaaatcttac tagtaaaatg ttaaatctta ctagcagtgt tgggtataat
     2341 ttgtaacaaa gttaaattcc atatctgcta agtgaaaaag taaagtaaga gattgctgta
     2401 aatatttagg taatttgttt tattaataca atatagaaag taacaaaaca caatgtaaaa
     2461 aaatcaataa agaaagtaaa taataaaagt aatgataaac taatgatcta aacaaaatta
     2521 gtaataaaat ttatacaatt cagggagggt aaagaatttc acctaatagt caaactctgt
     2581 attaaataat ataaaatatt tgttcttagt ctgaagagga gactgtgaga gaaagcttac
     2641 tggttattaa attagaaaat ttgtaacttg cagattaaag ctgtgtgcct tgcagattaa
     2701 aataatttaa ataatttaat aaaggccatg tttgagacaa atgtaaaaac ataataaatg
     2761 ttaagaattt cagatattga gattttacaa tttaaaaaaa gcaataaagt aatagcagag
     2821 acctacactc ttaaagtaaa ttactcaaca ctgcttacta gtaacttttg gaatagttac
     2881 tagtatccat tgaacaaata ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga
     2941 ttcttgtatt taaaacagta ccagtatcta tatatgtaca aaatttagtg ctagcaaaat
     3001 gcaaataaat actactgcat ataatggatt gcattgtaaa tcgacttgcc ataatacatt
     3061 tatttcctat ttttactttt aacaaaaaat aagttcactg agtatttcat gtcattaaat
     3121 cgcattaaac ctcttgcatg ctttatgtac actagtgcag ttcgctaaat gaccaccggt
     3181 gtcactaata acacgatcgt ttctgaattc tgcattaaac gccatttata cgttgctctt
     3241 attagtaaac aatttctcca tcatgagctc gaccagtatt gcatatttca tcctcttaaa
     3301 tccagagttg tttcttttaa tcactttctc ttcatgataa aagcagtttt tggttttcag
     3361 aagtttacaa caaacccagt cagttattgc ctcagcaaac actggagcaa ctgcacactt
     3421 gtgaaaagct tcttgcgaca caaacctttc tctggactta taaataaagg cattcagcag
     3481 ctccaccata cacctccact ggtcttgata aagggatcca aaaagggcga attcgaccca
     3541 agtttgtaca aaaaagcagg cttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     3601 actatagggc gaattgggta ccgggccccc cctcgagaat acaagctact tgttcttttt
     3661 gcaggatcca aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4381 agctgtacaa gtccggaaaa gagcgcccag agatctctct tccttcagac tttgagcata
     4441 cgattcatgt gggttttgat gcagtcaccg gggaattcac tggaattcct gagcaatggg
     4501 cacgactact ccaaacctcc aacataacaa agctggaaca gaagaagaac ccacaagctg
     4561 ttcttgatgt tctcaagttc tacgactcca aagaaacggt caacaaccag aaatctagaa
     4621 ctatagtgag tcgtattacg taggcggccg cggtggagct ccagcttttg ttccctttag
     4681 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc acccagcttt cttgtacaaa
     4741 gttgagatct ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat
     4801 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4861 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4921 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4981 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatcc
     5041 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagaggt ctctcaggtt
     5101 taaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttaa
     5161 acttgctatg ctgtttccag catagctctt aaacacaggg tctcgcattg gcccgaacta
     5221 ggagcctgga gaactgctat ataaacccgg gacttctggg tatgtttctg ccatgtggta
     5281 agtggataaa cctacttatt caactcattt acctcaaaca tgacagtaaa cctcttcggg
     5341 gcaaacctgc aaacgcttaa aaccttcaaa gtttgaaacc agcagttttg tagaggttta
     5401 ccgtagtatt tttgaactgt aaagcatatg cagaggaagc tggttttagg cttgtgtgat
     5461 tggatgagtt cttctgtgac gtaactgatc ttttgtcctc gtgtccttcg ccttccccca
     5521 caggcatgcg cggaccttcc cccgccgcag accagaggac aacatgctca gacgccagca
     5581 aacggcccat cgaaacattc acagactctc ggattggaga gaccgggccc gaggtctctg
     5641 actaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttattta
     5701 aacttgctat gctgtttcca gcatagctct taaacgggac tctgggatac agcccgaacc
     5761 aagagctgga gggagagcta tatataccag ggacttctgg gtatgttttt gggaggtggt
     5821 gagtgactaa accacttatt cagctccctt agatcaagtc tgaccctata tcatggtgac
     5881 ataaacctgc aaactgataa aacctgaagg atctcaaatc cagagtttgt gtgagggatt
     5941 accgtggtaa ttttcaaact gtaaagcata tgcaaaatta tctggtcttg gcttgagtga
     6001 ttgggtgtct cggtgtgatg cagggacgtt ttcagtgacg tgtccttctc cctcccccac
     6061 aggcatgcgc agaacatttc ccccctcctt gaagaccaga acaaaagacg ccgagagcag
     6121 gaaactcgtc ttactgaatg accgaggctg gagaaatcga ccctaggcaa ctttattata
     6181 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga
     6241 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     6301 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     6361 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     6421 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     6481 tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct
     6541 cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat
     6601 ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac
     6661 ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata
     6721 ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa
     6781 tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     6841 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     6901 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     6961 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     7021 agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     7081 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     7141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9241 aggggttccg cgcacatttc cccgaaaagt g
//
