LOCUS       Exported                9297 bp ds-DNA     circular SYN 31-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    lyzC-GFP-pa-U6CA-ctrlg-PBD.txt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 31, 2020 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1061..1081
                     /note="M13-rev"
     misc_recomb     complement(1097..1117)
                     /note="attB4"
     misc_feature    1145..3513
                     /note="lyzC promoter"
     primer_bind     3563..3580
                     /note="M13-fwd"
     primer_bind     3590..3609
                     /note="T7"
     CDS             3678..4391
                     /note="EGFP"
     primer_bind     complement(4672..4691)
                     /note="T3"
     polyA_signal    4794..4985
                     /note="SV40 late polyA"
     misc_feature    5195..5227
                     /note="ctrl guide"
     misc_feature    5749..5781
                     /note="ctrl guide"
     polyA_signal    6258..6449
                     /note="SV40 late polyA"
     primer_bind     complement(7111..7130)
                     /note="T3"
     primer_bind     complement(7148..7168)
                     /note="M13-rev"
     misc_binding    complement(7174..7196)
                     /note="LacO"
     promoter        complement(7201..7230)
                     /note="lac"
     rep_origin      complement(7536..8164)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(8316..8975)
                     /note="AmpR"
     promoter        complement(9215..9243)
                     /note="Amp prom"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tattgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgcttttta attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttttata
     1741 tcagtgtttt agattgaagt acaatattta taggtaaaat tcaccagcat atgcttaaag
     1801 atagccatgc tccaggtcct tgtgtccttc taaggtctgt ttgtggttta agctgtttgt
     1861 tgacacacgc ttgtcttctc catttttctg tgcagttatt gtcagtttat tgcacactgg
     1921 gttgcattaa gctgcagttt tttcacaaca gttttccaca ttgtaacagt cagtctccaa
     1981 attattccat cagtcctcaa atttagtcat ttgaggccat gaaaacactt aaaactacat
     2041 aaaggtagtt tggcattagg ttttagactt gttcttgttt ggcaatattg caacctgtgt
     2101 aaaataattt gattcttttt tttttttttt tttttttaat tttcagaaag aaatggtcca
     2161 aaaggagcta tgtgaacatt tatccatgtg tttaatgttt ttttttttat tattaaacca
     2221 cttctgactt aacttttttt agttattcta ttttgtgacg agtctgaatg gccaaagtac
     2281 ttttgaatcc aaaatcttac tagtaaaatg ttaaatctta ctagcagtgt tgggtataat
     2341 ttgtaacaaa gttaaattcc atatctgcta agtgaaaaag taaagtaaga gattgctgta
     2401 aatatttagg taatttgttt tattaataca atatagaaag taacaaaaca caatgtaaaa
     2461 aaatcaataa agaaagtaaa taataaaagt aatgataaac taatgatcta aacaaaatta
     2521 gtaataaaat ttatacaatt cagggagggt aaagaatttc acctaatagt caaactctgt
     2581 attaaataat ataaaatatt tgttcttagt ctgaagagga gactgtgaga gaaagcttac
     2641 tggttattaa attagaaaat ttgtaacttg cagattaaag ctgtgtgcct tgcagattaa
     2701 aataatttaa ataatttaat aaaggccatg tttgagacaa atgtaaaaac ataataaatg
     2761 ttaagaattt cagatattga gattttacaa tttaaaaaaa gcaataaagt aatagcagag
     2821 acctacactc ttaaagtaaa ttactcaaca ctgcttacta gtaacttttg gaatagttac
     2881 tagtatccat tgaacaaata ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga
     2941 ttcttgtatt taaaacagta ccagtatcta tatatgtaca aaatttagtg ctagcaaaat
     3001 gcaaataaat actactgcat ataatggatt gcattgtaaa tcgacttgcc ataatacatt
     3061 tatttcctat ttttactttt aacaaaaaat aagttcactg agtatttcat gtcattaaat
     3121 cgcattaaac ctcttgcatg ctttatgtac actagtgcag ttcgctaaat gaccaccggt
     3181 gtcactaata acacgatcgt ttctgaattc tgcattaaac gccatttata cgttgctctt
     3241 attagtaaac aatttctcca tcatgagctc gaccagtatt gcatatttca tcctcttaaa
     3301 tccagagttg tttcttttaa tcactttctc ttcatgataa aagcagtttt tggttttcag
     3361 aagtttacaa caaacccagt cagttattgc ctcagcaaac actggagcaa ctgcacactt
     3421 gtgaaaagct tcttgcgaca caaacctttc tctggactta taaataaagg cattcagcag
     3481 ctccaccata cacctccact ggtcttgata aagggatcca aaaagggcga attcgaccca
     3541 agtttgtaca aaaaagcagg cttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     3601 actatagggc gaattgggta ccgggccccc cctcgagaat acaagctact tgttcttttt
     3661 gcaggatcca aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4381 agctgtacaa gtccggaaaa gagcgcccag agatctctct tccttcagac tttgagcata
     4441 cgattcatgt gggttttgat gcagtcaccg gggaattcac tggaattcct gagcaatggg
     4501 cacgactact ccaaacctcc aacataacaa agctggaaca gaagaagaac ccacaagctg
     4561 ttcttgatgt tctcaagttc tacgactcca aagaaacggt caacaaccag aaatctagaa
     4621 ctatagtgag tcgtattacg taggcggccg cggtggagct ccagcttttg ttccctttag
     4681 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc acccagcttt cttgtacaaa
     4741 gttgagatct ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat
     4801 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4861 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4921 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4981 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatcc
     5041 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagaggt ctctcaggtt
     5101 taaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttaa
     5161 acttgctatg ctgtttccag catagctctt aaacagagac ggtcgacagt ctgcagtgtc
     5221 gtctctcgaa ctaggagcct ggagaactgc tatataaacc cgggacttct gggtatgttt
     5281 ctgccatgtg gtaagtggat aaacctactt attcaactca tttacctcaa acatgacagt
     5341 aaacctcttc ggggcaaacc tgcaaacgct taaaaccttc aaagtttgaa accagcagtt
     5401 ttgtagaggt ttaccgtagt atttttgaac tgtaaagcat atgcagagga agctggtttt
     5461 aggcttgtgt gattggatga gttcttctgt gacgtaactg atcttttgtc ctcgtgtcct
     5521 tcgccttccc ccacaggcat gcgcggacct tcccccgccg cagaccagag gacaacatgc
     5581 tcagacgcca gcaaacggcc catcgaaaca ttcacagact ctcggattgg agagaccggg
     5641 cccgaggtct ctgactaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
     5701 ctagccttat ttaaacttgc tatgctgttt ccagcatagc tcttaaacag agacggtcga
     5761 cagtctgcag tgtcgtctct cgaaccaaga gctggaggga gagctatata taccagggac
     5821 ttctgggtat gtttttggga ggtggtgagt gactaaacca cttattcagc tcccttagat
     5881 caagtctgac cctatatcat ggtgacataa acctgcaaac tgataaaacc tgaaggatct
     5941 caaatccaga gtttgtgtga gggattaccg tggtaatttt caaactgtaa agcatatgca
     6001 aaattatctg gtcttggctt gagtgattgg gtgtctcggt gtgatgcagg gacgttttca
     6061 gtgacgtgtc cttctccctc ccccacaggc atgcgcagaa catttccccc ctccttgaag
     6121 accagaacaa aagacgccga gagcaggaaa ctcgtcttac tgaatgaccg aggctggaga
     6181 aatcgaccct aggcaacttt attatacata gttgataatt cactggccgt cgttttacgg
     6241 taccatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
     6301 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6361 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6421 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     6481 ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc
     6541 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
     6601 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
     6661 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
     6721 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
     6781 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
     6841 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
     6901 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
     6961 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
     7021 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
     7081 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     7141 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7201 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7261 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7321 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
