LOCUS       Exported                9848 bp ds-DNA     circular SYN 31-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    lyzC-Utrophin-GFP-U6-ctrl guides.txt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 31, 2020 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1061..1081
                     /note="M13-rev"
     misc_recomb     complement(1097..1117)
                     /note="attB4"
     misc_feature    1145..3513
                     /note="LyzC promoter"
     primer_bind     3563..3580
                     /note="M13-fwd"
     primer_bind     3590..3609
                     /note="T7"
     misc_feature    3644..5194
                     /note="Utrophin GFP"
     CDS             4478..5191
                     /note="EGFP"
     primer_bind     complement(5223..5242)
                     /note="T3"
     polyA_signal    5345..5536
                     /note="SV40 late polyA"
     misc_feature    5746..5778
                     /note="ctrl guide"
     misc_feature    6300..6332
                     /note="ctrl guide"
     polyA_signal    6809..7000
                     /note="SV40 late polyA"
     primer_bind     complement(7662..7681)
                     /note="T3"
     primer_bind     complement(7699..7719)
                     /note="M13-rev"
     misc_binding    complement(7725..7747)
                     /note="LacO"
     promoter        complement(7752..7781)
                     /note="lac"
     rep_origin      complement(8087..8715)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(8867..9526)
                     /note="AmpR"
     promoter        complement(9766..9794)
                     /note="Amp prom"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tattgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgcttttta attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttttata
     1741 tcagtgtttt agattgaagt acaatattta taggtaaaat tcaccagcat atgcttaaag
     1801 atagccatgc tccaggtcct tgtgtccttc taaggtctgt ttgtggttta agctgtttgt
     1861 tgacacacgc ttgtcttctc catttttctg tgcagttatt gtcagtttat tgcacactgg
     1921 gttgcattaa gctgcagttt tttcacaaca gttttccaca ttgtaacagt cagtctccaa
     1981 attattccat cagtcctcaa atttagtcat ttgaggccat gaaaacactt aaaactacat
     2041 aaaggtagtt tggcattagg ttttagactt gttcttgttt ggcaatattg caacctgtgt
     2101 aaaataattt gattcttttt tttttttttt tttttttaat tttcagaaag aaatggtcca
     2161 aaaggagcta tgtgaacatt tatccatgtg tttaatgttt ttttttttat tattaaacca
     2221 cttctgactt aacttttttt agttattcta ttttgtgacg agtctgaatg gccaaagtac
     2281 ttttgaatcc aaaatcttac tagtaaaatg ttaaatctta ctagcagtgt tgggtataat
     2341 ttgtaacaaa gttaaattcc atatctgcta agtgaaaaag taaagtaaga gattgctgta
     2401 aatatttagg taatttgttt tattaataca atatagaaag taacaaaaca caatgtaaaa
     2461 aaatcaataa agaaagtaaa taataaaagt aatgataaac taatgatcta aacaaaatta
     2521 gtaataaaat ttatacaatt cagggagggt aaagaatttc acctaatagt caaactctgt
     2581 attaaataat ataaaatatt tgttcttagt ctgaagagga gactgtgaga gaaagcttac
     2641 tggttattaa attagaaaat ttgtaacttg cagattaaag ctgtgtgcct tgcagattaa
     2701 aataatttaa ataatttaat aaaggccatg tttgagacaa atgtaaaaac ataataaatg
     2761 ttaagaattt cagatattga gattttacaa tttaaaaaaa gcaataaagt aatagcagag
     2821 acctacactc ttaaagtaaa ttactcaaca ctgcttacta gtaacttttg gaatagttac
     2881 tagtatccat tgaacaaata ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga
     2941 ttcttgtatt taaaacagta ccagtatcta tatatgtaca aaatttagtg ctagcaaaat
     3001 gcaaataaat actactgcat ataatggatt gcattgtaaa tcgacttgcc ataatacatt
     3061 tatttcctat ttttactttt aacaaaaaat aagttcactg agtatttcat gtcattaaat
     3121 cgcattaaac ctcttgcatg ctttatgtac actagtgcag ttcgctaaat gaccaccggt
     3181 gtcactaata acacgatcgt ttctgaattc tgcattaaac gccatttata cgttgctctt
     3241 attagtaaac aatttctcca tcatgagctc gaccagtatt gcatatttca tcctcttaaa
     3301 tccagagttg tttcttttaa tcactttctc ttcatgataa aagcagtttt tggttttcag
     3361 aagtttacaa caaacccagt cagttattgc ctcagcaaac actggagcaa ctgcacactt
     3421 gtgaaaagct tcttgcgaca caaacctttc tctggactta taaataaagg cattcagcag
     3481 ctccaccata cacctccact ggtcttgata aagggatcca aaaagggcga attcgaccca
     3541 agtttgtaca aaaaagcagg cttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     3601 actatagggc gaattgggta ccgggccccc cctcgaggcc accatggcca agtatggaga
     3661 acatgaagcc agtcctgaca atgggcagaa cgaattcagt gatatcatta agtccagatc
     3721 tgatgaacac aatgacgtac agaagaaaac ctttaccaaa tggataaatg ctcgattttc
     3781 aaagagtggg aaaccaccca tcaatgatat gttcacagac ctcaaagatg gaaggaagct
     3841 attggatctt ctagaaggcc tcacaggaac atcactgcca aaggaacgtg gttccacaag
     3901 ggtacatgcc ttaaataacg tcaacagagt gctgcaggtt ttacatcaga acaatgtgga
     3961 attagtgaat atagggggaa ccgacattgt ggatggaaat cacaaactga ctttggggtt
     4021 actttggagc atcattttgc actggcaggt gaaagatgtc atgaaggatg tcatgtcgga
     4081 cctgcagcag acgaacagtg agaagatcct gctcagctgg gtgcgtcaga ccaccaggcc
     4141 ctacagccaa gtcaacgtcc tcaacttcac caccagctgg acagatggac tcgcctttaa
     4201 tgctgtcctc caccgacata aacctgatct cttcagctgg gataaagttg tcaaaatgtc
     4261 accaattgag agacttgaac atgccttcag caaggctcaa acttatttgg gaattgaaaa
     4321 gctgttagat cctgaagatg ttgccgttcg gcttcctgac aagaaatcca taattatgta
     4381 tttaacatct ttgtttgagg tgctacctca gcaagtcacc atagaccgaa ttctgcagtc
     4441 gacggtaccg cgggcccgtc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5161 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcggtggagc tccagctttt
     5221 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag cacccagctt
     5281 tcttgtacaa agttgagatc tataattcac tggccgtcgt tttacggtac catcgatgat
     5341 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5401 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5461 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5521 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     5581 cctctagatc ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggtctagagg
     5641 tctctcaggt ttaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
     5701 gccttattta aacttgctat gctgtttcca gcatagctct taaacagaga cggtcgacag
     5761 tctgcagtgt cgtctctcga actaggagcc tggagaactg ctatataaac ccgggacttc
     5821 tgggtatgtt tctgccatgt ggtaagtgga taaacctact tattcaactc atttacctca
     5881 aacatgacag taaacctctt cggggcaaac ctgcaaacgc ttaaaacctt caaagtttga
     5941 aaccagcagt tttgtagagg tttaccgtag tatttttgaa ctgtaaagca tatgcagagg
     6001 aagctggttt taggcttgtg tgattggatg agttcttctg tgacgtaact gatcttttgt
     6061 cctcgtgtcc ttcgccttcc cccacaggca tgcgcggacc ttcccccgcc gcagaccaga
     6121 ggacaacatg ctcagacgcc agcaaacggc ccatcgaaac attcacagac tctcggattg
     6181 gagagaccgg gcccgaggtc tctgactaaa aaagcaccga ctcggtgcca ctttttcaag
     6241 ttgataacgg actagcctta tttaaacttg ctatgctgtt tccagcatag ctcttaaaca
     6301 gagacggtcg acagtctgca gtgtcgtctc tcgaaccaag agctggaggg agagctatat
     6361 ataccaggga cttctgggta tgtttttggg aggtggtgag tgactaaacc acttattcag
     6421 ctcccttaga tcaagtctga ccctatatca tggtgacata aacctgcaaa ctgataaaac
     6481 ctgaaggatc tcaaatccag agtttgtgtg agggattacc gtggtaattt tcaaactgta
     6541 aagcatatgc aaaattatct ggtcttggct tgagtgattg ggtgtctcgg tgtgatgcag
     6601 ggacgttttc agtgacgtgt ccttctccct cccccacagg catgcgcaga acatttcccc
     6661 cctccttgaa gaccagaaca aaagacgccg agagcaggaa actcgtctta ctgaatgacc
     6721 gaggctggag aaatcgaccc taggcaactt tattatacat agttgataat tcactggccg
     6781 tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga tgagtttgga
     6841 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6901 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6961 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7021 aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaagatac ggccacgggt
     7081 gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata
     7141 cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt
     7201 gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt
     7261 ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat
     7321 taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag
     7381 tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac
     7441 ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc
     7501 aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca
     7561 agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag
     7621 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     7681 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7741 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7801 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7861 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7921 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7981 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8041 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8101 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8161 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8221 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8281 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8341 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8401 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8461 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8521 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8581 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8641 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8701 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8761 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9721 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9781 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9841 gaaaagtg
//
