LOCUS       lyzC-Utrophin-GF        9822 bp DNA     circular SYN 03-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1061..1081
                     /label=M13-rev
     misc_recomb     complement(1097..1117)
                     /label=attB4
     misc_feature    1145..3513
                     /label=LyzC promoter
     primer_bind     3563..3580
                     /label=M13-fwd
     primer_bind     3590..3609
                     /label=T7
     misc_feature    3644..5194
                     /label=Utrophin GFP
     CDS             4478..5191
                     /label=EGFP
     primer_bind     complement(5223..5242)
                     /label=T3
     polyA_signal    5345..5536
                     /label=SV40 late polyA
     misc_feature    5746..5765
                     /label=rac guide
     misc_feature    6287..6306
                     /label=rac guide
     polyA_signal    6783..6974
                     /label=SV40 late polyA
     primer_bind     complement(7636..7655)
                     /label=T3
     primer_bind     complement(7673..7693)
                     /label=M13-rev
     misc_binding    complement(7699..7721)
                     /label=LacO
     promoter        complement(7726..7755)
                     /label=lac
     rep_origin      complement(8061..8689)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8841..9500)
                     /label=AmpR
     promoter        complement(9740..9768)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tattgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgcttttta attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttttata
     1741 tcagtgtttt agattgaagt acaatattta taggtaaaat tcaccagcat atgcttaaag
     1801 atagccatgc tccaggtcct tgtgtccttc taaggtctgt ttgtggttta agctgtttgt
     1861 tgacacacgc ttgtcttctc catttttctg tgcagttatt gtcagtttat tgcacactgg
     1921 gttgcattaa gctgcagttt tttcacaaca gttttccaca ttgtaacagt cagtctccaa
     1981 attattccat cagtcctcaa atttagtcat ttgaggccat gaaaacactt aaaactacat
     2041 aaaggtagtt tggcattagg ttttagactt gttcttgttt ggcaatattg caacctgtgt
     2101 aaaataattt gattcttttt tttttttttt tttttttaat tttcagaaag aaatggtcca
     2161 aaaggagcta tgtgaacatt tatccatgtg tttaatgttt ttttttttat tattaaacca
     2221 cttctgactt aacttttttt agttattcta ttttgtgacg agtctgaatg gccaaagtac
     2281 ttttgaatcc aaaatcttac tagtaaaatg ttaaatctta ctagcagtgt tgggtataat
     2341 ttgtaacaaa gttaaattcc atatctgcta agtgaaaaag taaagtaaga gattgctgta
     2401 aatatttagg taatttgttt tattaataca atatagaaag taacaaaaca caatgtaaaa
     2461 aaatcaataa agaaagtaaa taataaaagt aatgataaac taatgatcta aacaaaatta
     2521 gtaataaaat ttatacaatt cagggagggt aaagaatttc acctaatagt caaactctgt
     2581 attaaataat ataaaatatt tgttcttagt ctgaagagga gactgtgaga gaaagcttac
     2641 tggttattaa attagaaaat ttgtaacttg cagattaaag ctgtgtgcct tgcagattaa
     2701 aataatttaa ataatttaat aaaggccatg tttgagacaa atgtaaaaac ataataaatg
     2761 ttaagaattt cagatattga gattttacaa tttaaaaaaa gcaataaagt aatagcagag
     2821 acctacactc ttaaagtaaa ttactcaaca ctgcttacta gtaacttttg gaatagttac
     2881 tagtatccat tgaacaaata ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga
     2941 ttcttgtatt taaaacagta ccagtatcta tatatgtaca aaatttagtg ctagcaaaat
     3001 gcaaataaat actactgcat ataatggatt gcattgtaaa tcgacttgcc ataatacatt
     3061 tatttcctat ttttactttt aacaaaaaat aagttcactg agtatttcat gtcattaaat
     3121 cgcattaaac ctcttgcatg ctttatgtac actagtgcag ttcgctaaat gaccaccggt
     3181 gtcactaata acacgatcgt ttctgaattc tgcattaaac gccatttata cgttgctctt
     3241 attagtaaac aatttctcca tcatgagctc gaccagtatt gcatatttca tcctcttaaa
     3301 tccagagttg tttcttttaa tcactttctc ttcatgataa aagcagtttt tggttttcag
     3361 aagtttacaa caaacccagt cagttattgc ctcagcaaac actggagcaa ctgcacactt
     3421 gtgaaaagct tcttgcgaca caaacctttc tctggactta taaataaagg cattcagcag
     3481 ctccaccata cacctccact ggtcttgata aagggatcca aaaagggcga attcgaccca
     3541 agtttgtaca aaaaagcagg cttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     3601 actatagggc gaattgggta ccgggccccc cctcgaggcc accatggcca agtatggaga
     3661 acatgaagcc agtcctgaca atgggcagaa cgaattcagt gatatcatta agtccagatc
     3721 tgatgaacac aatgacgtac agaagaaaac ctttaccaaa tggataaatg ctcgattttc
     3781 aaagagtggg aaaccaccca tcaatgatat gttcacagac ctcaaagatg gaaggaagct
     3841 attggatctt ctagaaggcc tcacaggaac atcactgcca aaggaacgtg gttccacaag
     3901 ggtacatgcc ttaaataacg tcaacagagt gctgcaggtt ttacatcaga acaatgtgga
     3961 attagtgaat atagggggaa ccgacattgt ggatggaaat cacaaactga ctttggggtt
     4021 actttggagc atcattttgc actggcaggt gaaagatgtc atgaaggatg tcatgtcgga
     4081 cctgcagcag acgaacagtg agaagatcct gctcagctgg gtgcgtcaga ccaccaggcc
     4141 ctacagccaa gtcaacgtcc tcaacttcac caccagctgg acagatggac tcgcctttaa
     4201 tgctgtcctc caccgacata aacctgatct cttcagctgg gataaagttg tcaaaatgtc
     4261 accaattgag agacttgaac atgccttcag caaggctcaa acttatttgg gaattgaaaa
     4321 gctgttagat cctgaagatg ttgccgttcg gcttcctgac aagaaatcca taattatgta
     4381 tttaacatct ttgtttgagg tgctacctca gcaagtcacc atagaccgaa ttctgcagtc
     4441 gacggtaccg cgggcccgtc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5161 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcggtggagc tccagctttt
     5221 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag cacccagctt
     5281 tcttgtacaa agttgagatc tataattcac tggccgtcgt tttacggtac catcgatgat
     5341 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5401 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5461 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5521 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     5581 cctctagatc ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggtctagagg
     5641 tctctcaggt ttaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
     5701 gccttattta aacttgctat gctgtttcca gcatagctct taaacacagg gtctcgcatt
     5761 ggcccgaact aggagcctgg agaactgcta tataaacccg ggacttctgg gtatgtttct
     5821 gccatgtggt aagtggataa acctacttat tcaactcatt tacctcaaac atgacagtaa
     5881 acctcttcgg ggcaaacctg caaacgctta aaaccttcaa agtttgaaac cagcagtttt
     5941 gtagaggttt accgtagtat ttttgaactg taaagcatat gcagaggaag ctggttttag
     6001 gcttgtgtga ttggatgagt tcttctgtga cgtaactgat cttttgtcct cgtgtccttc
     6061 gccttccccc acaggcatgc gcggaccttc ccccgccgca gaccagagga caacatgctc
     6121 agacgccagc aaacggccca tcgaaacatt cacagactct cggattggag agaccgggcc
     6181 cgaggtctct gactaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
     6241 agccttattt aaacttgcta tgctgtttcc agcatagctc ttaaacggga ctctgggata
     6301 cagcccgaac caagagctgg agggagagct atatatacca gggacttctg ggtatgtttt
     6361 tgggaggtgg tgagtgacta aaccacttat tcagctccct tagatcaagt ctgaccctat
     6421 atcatggtga cataaacctg caaactgata aaacctgaag gatctcaaat ccagagtttg
     6481 tgtgagggat taccgtggta attttcaaac tgtaaagcat atgcaaaatt atctggtctt
     6541 ggcttgagtg attgggtgtc tcggtgtgat gcagggacgt tttcagtgac gtgtccttct
     6601 ccctccccca caggcatgcg cagaacattt cccccctcct tgaagaccag aacaaaagac
     6661 gccgagagca ggaaactcgt cttactgaat gaccgaggct ggagaaatcg accctaggca
     6721 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     6781 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6841 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6901 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6961 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     7021 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     7081 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     7141 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     7201 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     7261 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     7321 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     7381 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     7441 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     7501 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     7561 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     7621 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7981 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8581 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8641 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8701 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8761 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8821 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8881 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8941 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9001 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9061 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9121 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9181 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9241 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9301 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9361 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9421 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9481 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9541 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9601 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9661 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9721 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9781 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
