LOCUS       pMRBad-Z-C-wtBlc        4828 bp ds-DNA     circular     07-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..4828
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3..326
                     /label="Zip-C-wtBlc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             3..326
                     /label="Translation 3-326"
     primer_bind     complement(388..406)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      402..449
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(1051..1866)
                     /label="KanR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1774..1793
                     /label="Kan-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1858..1877
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2327..2344)
                     /label="L4440"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(2461..3005)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(2541..2560)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(3585..4463)
                     /label="araC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        4490..4774
                     /label="araBAD promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     4697..4716
                     /label="pBAD Forward"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     RBS             4801..4823
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ccATGGCAAG CGAGCAGCTG GAAAAGAAGT TACAAGCCCT GGAGAAAAAA CTTGCTCAGC
       61 TGGAATGGAA AAACCAAGCA TTGGAAAAAA AACTCGCGCA GACGTCGGGT GGAAGCGGTG
      121 GTCCTTTCTA TGGCGGTTAT AACGTTATTG CACTCGATCG GGAATACCGC CATGCGCTGG
      181 TTTGCGGCCC GGACCGCGAC TACCTGTGGA TACTCTCCCG CACGCCAACC ATTTCTGACG
      241 AAGTGAAACA GGAGATGCTG GCAGTCGCTA CCCGGGAAGG GTTTGATGTC AGTAAATTTA
      301 TTTGGGTACA GCAGCCTGGT AGTTAGtgta caactaagga tccggctgct aacaaagccc
      361 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      421 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
      481 aggacgggtg tggtcgccat gatggtgacc accatttgcc ccatatcacc gtcggtactg
      541 atcccgtcgt caataaaccg aaccgctaca ccctgagcat caaactcttt tatcagttgg
      601 atcatgtcgg cggtgtcgcg gccaagacgg tcgagcttct tcaccagaat gacatcacct
      661 tcctccacct tcatcctcag caaatccagc ccttcccgat ctgttgaact gccggatgcc
      721 ttgtcggtaa agatgcggtt agcttttacc cctgcatctt tgagcgctga ggtctgcctc
      781 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
      841 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
      901 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
      961 cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg
     1021 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1081 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1141 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1201 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1261 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     1321 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     1381 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     1441 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     1501 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     1561 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1621 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1681 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1741 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     1801 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg
     1861 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     1921 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg
     1981 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     2041 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     2101 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
     2161 gtcagcaaca ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac
     2221 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     2281 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     2341 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     2401 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     2461 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     2521 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc
     2581 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     2641 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     2701 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2761 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     2821 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     2881 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     2941 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     3001 ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3061 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3481 ttgctgcaga tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc
     3541 ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
     3601 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
     3661 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
     3721 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
     3781 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
     3841 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
     3901 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
     3961 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc
     4021 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
     4081 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     4141 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     4201 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     4261 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     4321 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     4381 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     4441 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
     4501 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     4561 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     4621 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     4681 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga
     4741 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
     4801 tttgtttaac tttaagaagg agatatac
//