LOCUS       pET11a-Z-N-wtBlc        6076 bp ds-DNA     circular     07-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..6076
                     /label="source"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3..437
                     /label="N-wtBlc-Zip"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3..437
                     /label="Translation 3-437"
     primer_bind     complement(488..506)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      502..549
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(724..752)
                     /label="tet promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(779..797)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        865..969
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             970..1830
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(1188..1207)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      2001..2589
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     2490..2509
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     2743..2760
                     /label="L4440"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2775..2917
                     /label="bom"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2843..2862)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     3003..3025
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(3019..3210)
                     /label="rop"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(4522..5604)
                     /label="lacI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     5566..5585
                     /label="LacI-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(5605..5682)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     5888..5907
                     /label="pBRrevBam"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        5991..6009
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     5991..6010
                     /label="T7"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    6010..6034
                     /label="lac operator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             6049..6071
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 atATGGGTCA TCACCACCAC CATCACCTCG AATCAACCAG CTTGTACAAG AAATCCAGCT
       61 CCACGCCGCC GCGTGGCGTG ACCGTAGTAA ATAATTTCGA CGCCAAACGT TATCTCGGTA
      121 CCTGGTATGA GATTGCCCGT TTTGATCACC GCTTTGAACG TGGACTGGAA AAAGTCACCG
      181 CAACATACAG CCTGCGTGAT GACGGCGGCC TGAATGTCAT TAATAAAGGC TATAACCCTG
      241 ACAGAGGAAT GTGGCAGCAG AGTGAAGGGA AAGCGTACTT TACCGGCGCA CCAACTCGCG
      301 CTGCGCTGAA AGTGTCATTC TTTGGTGGCT CTGGCTCTGG CTCGAGCGCC CTCAAAAAAG
      361 AATTGCAGGC AAACAAAAAA GAACTTGCGC AGCTGAAGTG GGAGTTACAA GCTCTGAAAA
      421 AGGAACTGGC GCAGTAAgga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      481 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      541 ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg
      601 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      661 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      721 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
      781 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1201 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1501 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2761 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2821 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     2881 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     2941 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3001 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     3061 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     3121 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     3181 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     3241 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     3301 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     3361 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     3421 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     3481 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     3541 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     3601 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     3661 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     3721 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     3781 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
     3841 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     3901 acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg
     3961 gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg
     4021 agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     4081 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     4141 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata
     4201 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     4261 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     4321 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     4381 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     4441 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     4501 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4561 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     4621 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     4681 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     4741 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     4801 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     4861 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     4921 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     4981 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     5041 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     5101 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     5161 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     5221 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     5281 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     5341 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     5401 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     5461 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     5521 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     5581 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     5641 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     5701 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     5761 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     5821 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     5881 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     5941 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
     6001 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
     6061 taagaaggag atatac
//