LOCUS       Exported               15588 bp ds-DNA     linear   SYN 08-MAR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 8, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     656..668
                     /label=AGATGGGTGAGGTGGAGTACGCG  366A
                     /note="color: black; sequence: AGATGGGTGAGGTGGAGTACGCG366A"
     CDS             complement(854..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  854 .. 1645 / #ccffcc
                        2: 1646 .. 1714 / #ccffcc / signal sequence
                     Cleavage site after base 1645"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
                     /note="color: #f7a68a; direction: LEFT"
     misc_binding    4073..4357
                     /label=attB site
                     /note="attB site"
                     /note="color: #f7a68a"
     primer_bind     4073..4096
                     /label=GTCGACGATGTAGGTCACGGTCTC
                     /note="color: black; sequence: GTCGACGATGTAGGTCACGGTCTC"
     primer_bind     complement(4079..4102)
                     /label=attBseqR Primer 191
                     /note="attBseqR Primer 191"
                     /note="color: black; sequence: ggcttcgagaccgtgacctacatc"
     primer_bind     complement(4340..4357)
                     /label=GTCGACATGCCCGCCGTG
                     /note="color: black; sequence: GTCGACATGCCCGCCGTG"
     misc_feature    4366..7196
                     /label=BicC promoter predicted
                     /note="BicC promoter predicted"
                     /note="color: #80ff80; direction: RIGHT"
     primer_bind     4366..4397
                     /label=ATAATTATATAATAATAAACTGCATGCCGCCA
                     /note="color: black; sequence: 
                     ATAATTATATAATAATAAACTGCATGCCGCCA"
     primer_bind     complement(7171..7196)
                     /label=TGTGGAATTCGGATGATGATGATGAT
                     /note="color: black; sequence: TGTGGAATTCGGATGATGATGATGAT"
     primer_bind     7205..7229
                     /label=ATGGACTATAAGGACCACGACGGAG
                     /note="color: black; sequence: ATGGACTATAAGGACCACGACGGAG"
     CDS             7208..7273
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 7273"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7280..7300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7325..11425
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     8230..8267
                     /label=CGACATCCTGAGAGTGAACACCGAG 843C
                     /note="color: black; sequence: 
                     CGACATCCTGAGAGTGAACACCGAG843C"
     primer_bind     complement(9061..9071)
                     /label=ATGGACTATAAGGACCACGACGGAG
                     /note="color: black; sequence: ATGGACTATAAGGACCACGACGGAG"
     primer_bind     9728..9740
                     /label=CGACATCCTGAGAGTGAACACCGAG 843C
                     /note="color: black; sequence: 
                     CGACATCCTGAGAGTGAACACCGAG843C"
     primer_bind     complement(10288..10297)
                     /label=TGATGAACTTCGAGGACGGC  644C
                     /note="color: black; sequence: TGATGAACTTCGAGGACGGC644C"
     primer_bind     10613..10622
                     /label=CGACATCCTGAGAGTGAACACCGAG 843C
                     /note="color: black; sequence: 
                     CGACATCCTGAGAGTGAACACCGAG843C"
     CDS             11426..11473
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(11452..11473)
                     /label=TTACTTTTTCTTTTTTGCCTGGCCG
                     /note="color: black; sequence: TTACTTTTTCTTTTTTGCCTGGCCG"
     CDS             11474..11527
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 11524"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11528..12247
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 11528 .. 11530 / #05fd14
                        2: 11531 .. 11533 / #05fd14 / 1a
                        3: 11534 .. 12247 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     11528..11548
                     /label=ATGGTGAGCAAGGGCGAGGAG
                     /note="color: black; sequence: ATGGTGAGCAAGGGCGAGGAG"
     primer_bind     11648..11677
                     /label=GGCAAGCTGACCCTGAAGTTCATCTGC  208
                     /note="color: black; sequence: 
                     GGCAAGCTGACCCTGAAGTTCATCTGC208"
     primer_bind     complement(12082..12111)
                     /label=AGCACGGGGCCGTCGCCGATGGGGGTG  209
                     /note="color: black; sequence: 
                     AGCACGGGGCCGTCGCCGATGGGGGTG209"
     primer_bind     complement(12224..12247)
                     /label=TTACTTGTACAGCTCGTCCATGCC
                     /note="color: black; sequence: TTACTTGTACAGCTCGTCCATGCC"
     primer_bind     12256..12287
                     /label=CTAGAATGAATCGTTTTTAAAATAACAAATCA
                     /note="color: black; sequence: 
                     CTAGAATGAATCGTTTTTAAAATAACAAATCA"
     3'UTR           12261..12920
                     /label=p10 3'UTR
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(12913..12932)
                     /label=CCGGCCGTTAACTCGAATCG
                     /note="color: black; sequence: CCGGCCGTTAACTCGAATCG"
     misc_feature    12941..13481
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #ff8000; direction: RIGHT"
     primer_bind     12941..12961
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(13376..13402)
                     /label=AACAGATGCTGTTCAACTGTGTTTACC
                     /note="color: black; sequence: AACAGATGCTGTTCAACTGTGTTTACC"
     primer_bind     13404..13425
                     /label=TACAGCGACACAACATGAGCCG  777C
                     /note="color: black; sequence: TACAGCGACACAACATGAGCCG777C"
     primer_bind     complement(13428..13446)
                     /label=TGGGCGCGTTGGCGTTGTT
                     /note="color: black; sequence: TGGGCGCGTTGGCGTTGTT"
     primer_bind     complement(13462..13481)
                     /label=CACCAGAGACAGGTTGCGGC
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGGC"
     CDS             13482..14162
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 13482 .. 13484 / #ff0000
                        2: 13485 .. 13487 / #ff0000 / 1a
                        3: 13488 .. 14162 / #ff0000"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     13483..13505
                     /label=ATGGTGCGCTCCTCCAAGAAC 644A
                     /note="color: black; sequence: ATGGTGCGCTCCTCCAAGAAC644A"
     misc_feature    13809..13817
                     /label=pt mutation
                     /note="pt mutation"
                     /note="color: #ff8000"
     primer_bind     13849..13865
                     /label=GGCAAGCTGACCCTGAAGTTCATCTGC  208
                     /note="color: black; sequence: 
                     GGCAAGCTGACCCTGAAGTTCATCTGC208"
     primer_bind     complement(13934..13952)
                     /label=AGCACGGGGCCGTCGCCGATGGGGGTG  209
                     /note="color: black; sequence: 
                     AGCACGGGGCCGTCGCCGATGGGGGTG209"
     misc_feature    14163..14403
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #ff8000; direction: RIGHT"
     primer_bind     14163..14189
                     /label=GACTCTAGATCATAATCAGCCATACCA
                     /note="color: black; sequence: GACTCTAGATCATAATCAGCCATACCA"
     primer_bind     14355..14381
                     /label=CTGCATTCTAGTTGTGGTTTGTCCA 175
                     /note="color: black; sequence: 
                     CTGCATTCTAGTTGTGGTTTGTCCA175"
     primer_bind     complement(14374..14403)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
                     /note="color: black; sequence: 
                     AAGCTTTAAGATACATTGATGAGTTTGGAC"
     misc_binding    14415..15115
                     /label=piggyBacL
                     /note="color: #f7a68a"
     primer_bind     complement(15281..15297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    15305..15321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(15329..15359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 15329 .. 15335 / #ffffff / -10
                        2: 15336 .. 15353 / #ffffff
                        3: 15354 .. 15359 / #ffffff / -35"
     protein_bind    15374..15395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggtcgacga
     4081 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     4141 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
     4201 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
     4261 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
     4321 tcagcgggtt ctcgacggtc acggcgggca tgtcgacgcg gccgcataat tatataataa
     4381 taaactgcat gccgccagat ttttgtaatc aaatcgtgtt gtgaaagttg tcttccctaa
     4441 tttatgccca ttacattaaa acattgacag gggattttaa ttttaatttc agtatggttt
     4501 caattatggc caatatatat aataaactat aaccatatgt ataagaatgt aaaaagctag
     4561 ttttatgtac ataaaaatgg gacaaacatt ttgatgttac tgaataaata ttaaaagtat
     4621 taaccaagaa atatgaaagt aggataaaaa aacagtgaat gtttccagct gctaggaaaa
     4681 acgataagtc ttagatctat aaagagaatc aaaaacgcgg tcaaatgcat agcacataat
     4741 atattcatat ccccaataaa taacccgaaa tgtgtaaagc cagaataaat gtaataataa
     4801 gattgaagaa aacaaaatag tgatgtaaaa tagcagcaag gaaataaagt aacccctatt
     4861 caataactat tgctgacgtc ttttactcgt acatgtcatc atttaattac gtaatttaag
     4921 tttggtttat ttaaagtttg atttccggtt gtacaagggt gagcgtactt ttaaaaccca
     4981 ctgtatataa atactttggt aaagtgcagg ttggggagtt tcagaagctt gaaaagctcc
     5041 gtttcccgtt ataaagctat tgctgaatat ccgttatcca gttggctggc agctttttaa
     5101 ggttgttcgt gcactggctt cactccccac agaacacaga gcttttggat ctgaaggtca
     5161 gttgggactg ggacccacat gccgagctta gtttgatagt cagcgcgctt taatgacgac
     5221 aactgcacgg cgcgcacaac ccattaataa tatattaatt caaaagagct ataaaatatt
     5281 aaaaaatatt caaatataac taactaacgc gcagtgcacg attttctgac aacaattcag
     5341 tttcgctttt catgagactc cattgcattt tcctttgtgt ttttcacaca cgtggtgttt
     5401 ttctgttact cgcacttcga tagtggttat tttttttggt tacattgaaa aaatacaccg
     5461 actgccagcc gaattcccat tcgaacctta atttgaccaa ttgacggaaa attaattgcg
     5521 cccaaaatta attagcaagc ggatatacaa tttttttacg tgaaaagagc gtgttatttg
     5581 tcgaaaagat tttacaattg attgtgttta agtaaattaa cctgcgatat ctatgtgtat
     5641 gtgctacgat ccgaatttct gacattacgg taagtggcgt gtttttggcg ggaataaagt
     5701 cggtttctct ttgtgccgta tttctctacc gttttttctg gcgccaaaat gaatgaaaac
     5761 ggttggcaac atttcaaact taacattgca acattctaac cgctgttaag cgtttgctct
     5821 gcaaattgtt gtagctatgt tggagttctg gaacaccacc tctccctgcg actttgtttt
     5881 gtttttcgct ctcgacatat ggaatcgaat ctcttattcc gggagcggtt cattgaccca
     5941 gcaacaacaa tattcgccag attttctcct gccagcgatt tgcggaattc atccgctcaa
     6001 cagttagaag taaatgccgt cgagcagcgg gcaaaatcga tcgacgaaat cgagagccgc
     6061 gttcagttgc cttcaagccg tacttagaag aagtgctggt cccccagaaa tcagagagat
     6121 ttttcccaac ttgcattttt ccaatactct gacaaagccc ctcagaccgt cgcaatcaca
     6181 cagctatata ctgatatata gatctctatt gcgcagatca caccttaaat gcctttcgtc
     6241 gagagatttc aacaaatacg taattttgtt atgaacgtct gcctcgtcga ggccatccaa
     6301 ttcgtccagg acctctcgca cgccatctgt ctggacctcc agggcctatt taatctcaag
     6361 tagagatcac cgagcgatct cccagcggta agttacgcca ttggtttttg ggcacaagga
     6421 agtgaaagtg aagacacggg tgaaattacg gttttcggga tctcgagaga aaggggaaag
     6481 tacgaacctc atggcagcag cactccattt tgagaggtta tgtgaaagaa cacacaagaa
     6541 agtttggaat tttcgacagg cggcgaacca ataataatgt tgttatccat aataatatta
     6601 attagtgaat ttttatttca ccttttcgcg gcattattct cacaaattta taagacagat
     6661 tttcaaatta agacagaaag ttttttaatg ttagtgaaaa gtacttattc cagttgatag
     6721 ttaaatacta tttaccgcaa aaaattaaaa gagaactaaa tttaaaggta aaatcgtttt
     6781 tttttttgga aagtgcattg cttaaattat ggtaccgact atcaaaataa aaataattaa
     6841 tttttatatg gtgtttcaat attcacttta aattacctat gactcgcttt ctaaccactc
     6901 tcttcttatt acgaacatca attttcttgt tacatcattt cggacctttc atttttcatc
     6961 accagctcat cccatcaatt cagatacact ttacttcatt aagtagcagc aatcatctaa
     7021 caaagctgac caaacaacgg accggcttcc agaatcctta actgagacgg acgttttgct
     7081 tggtttttaa aactaaacga ctttttggta cccacacctg ttgacctctg acgaatttct
     7141 cgatccactg accgatccac tgatccagtg atcatcatca tcatccgaat tccacagttt
     7201 aaacatggac tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga
     7261 cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc
     7321 agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
     7381 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     7441 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     7501 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     7561 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     7621 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     7681 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     7741 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     7801 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     7861 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     7921 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     7981 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     8041 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     8101 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     8161 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     8221 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     8281 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     8341 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     8401 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     8461 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     8521 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     8581 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     8641 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     8701 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     8761 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     8821 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     8881 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     8941 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     9001 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     9061 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     9121 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     9181 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     9241 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     9301 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     9361 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     9421 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     9481 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     9541 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     9601 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     9661 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     9721 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     9781 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca
     9841 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     9901 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     9961 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
    10021 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
    10081 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
    10141 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
    10201 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
    10261 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
    10321 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
    10381 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
    10441 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
    10501 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
    10561 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
    10621 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
    10681 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
    10741 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
    10801 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
    10861 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
    10921 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
    10981 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
    11041 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
    11101 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
    11161 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
    11221 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
    11281 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
    11341 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
    11401 cgacctgtct cagctgggag gcgacaaaag gccggcggcc acgaaaaagg ccggccaggc
    11461 aaaaaagaaa aaggagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
    11521 cggccctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
    11581 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
    11641 cacctacggc aagctgaccc tgaagttcat ctgcaccacc agcaagctgc ccgtgccctg
    11701 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
    11761 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
    11821 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
    11881 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
    11941 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
    12001 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
    12061 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
    12121 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
    12181 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
    12241 caagtaatta attaactaga atgaatcgtt tttaaaataa caaatcaatt gttttataat
    12301 attcgtacga ttctttgatt atgtaataaa atgtgatcat taggaagatt acgaaaaata
    12361 taaaaaatat gagttctgtg tgtataacaa atgctgtaaa cgccacaatt gtgtttgttg
    12421 caaataaacc catgattatt tgattaaaat tgttgttttc tttgttcata gacaatagtg
    12481 tgttttgcct aaacgtgtac tgcataaact ccatgcgagt gtatagcgag ctagtggcta
    12541 acgcttgccc caccaaagta gattcgtcaa aatcctcaat ttcatcaccc tcctccaagt
    12601 ttaacatttg gccgtcggaa ttaacttcta aagatgccac ataatctaat aaatgaaata
    12661 gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc cgaaaagaac tcgggcataa
    12721 actctatgat ttctctggac gtggtgttgt cgaaactctc aaagtacgca gtcaggaacg
    12781 tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt gttgtaaccg aacgggtccc
    12841 atagcgccaa aaccaaatct gccagcgtca atagaatgag cacgatgccg acaatggagc
    12901 tggcttggat agcgattcga gttaacggcc ggggcgcgcc catactcggt ggcctcccca
    12961 ccaccaactt ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac
    13021 aaactctacg tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa
    13081 atacactacc acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt
    13141 aggccggcct tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag
    13201 tgttgtctta tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac
    13261 tccttatcgg aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg
    13321 gacacgaggc gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa
    13381 acacagttga acagcatctg ttacagcgac acaacatgag ccggtccaac aacgccaacg
    13441 cgcccacgcc atccaaccgc cgccgcaacc tgtctctggt gatggtgcgc tcctccaaga
    13501 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg
    13561 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc
    13621 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
    13681 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
    13741 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
    13801 ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg
    13861 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
    13921 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
    13981 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
    14041 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
    14101 aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt
    14161 aggactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    14221 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    14281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    14341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag
    14401 cttctaggcc ggccgatctc ggatctgaca atgttcagtg cagagactcg gctacgcctc
    14461 gtggactttg aagttgacca acaatgttta ttcttacctc taatagtcct ctgtggcaag
    14521 gtcaagattc tgttagaagc caatgaagaa cctggttgtt caataacatt ttgttcgtct
    14581 aatatttcac taccgcttga cgttggctgc acttcatgta cctcatctat aaacgcttct
    14641 tctgtatcgc tctggacgtc atcttcactt acgtgatctg atatttcact gtcagaatcc
    14701 tcaccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa
    14761 gaactaccca ttttattata tattagtcac gatatctata acaagaaaat atatatataa
    14821 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt
    14881 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa
    14941 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag
    15001 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag
    15061 aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta
    15121 ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc
    15181 aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga gcgtcgactc
    15241 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt
    15301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    15361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    15421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    15481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    15541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggt
//
