LOCUS       Exported                4974 bp ds-DNA     circular SYN 11-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHEA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 9, 2021 from SnapGene Viewer 5.2.1
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5832 bp ds-DNA     circular SYN 
            21-ABR-2016
            DEFINITION  synthetic circular DNA
            ACCESSION   .
            VERSION     .
            KEYWORDS    pAK-Backbone1
            SOURCE      synthetic DNA construct
              ORGANISM  synthetic DNA construct
            REFERENCE   1  (bases 1 to 5832)
              AUTHORS   .
              TITLE     Direct Submission
              JOURNAL   Exported 8 de may de 2016 from SnapGene Viewer 3.0.2
                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="synthetic DNA construct"
                     /label=synthetic DNA construct
     promoter        5..82
                     /label=lacI promoter
                     /note="lacI promoter"
     CDS             83..1165
                     /codon_start=1
                     /label=lacI repressor
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    1233..1254
                     /label=E. coli catabolite activator protein
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1269..1299
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1307..1323
                     /label=lac repressor encoded by lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1331..1347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1383..2606
                     /codon_start=1
                     /label=HEA
                     /translation="MKYLLPTAAAGLLLLAAQPAMAHHHHHHAAAAGAEFVDGAPVPYP
                     DPLEPIDNSAAISMANPRPPTPRTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRL
                     HERLGNTYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWS
                     QNGSDRWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLD
                     SWAQYSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQ
                     VTWMGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNW
                     LHNSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSA
                     MVGIKWQF"
     misc_feature    1383..1442
                     /label=pelB signal peptide
                     /note="pelB signal peptide"
     misc_feature    1449..1466
                     /label=6xHis
                     /note="6xHis"
     misc_feature    1497..1532
                     /label=E-tag
                     /note="E-tag"
     misc_feature    1587..2606
                     /label=Autotransporter b-domain C-EhaA
                     /note="Autotransporter b-domain C-EhaA "
     misc_feature    1640..1663
                     /label=Oligo ALOH-IgAP1
                     /note="Oligo ALOH-IgAP1"
     misc_feature    1641..1721
                     /label=Alpha helix
                     /note="Alpha helix"
     misc_feature    1722..1742
                     /label=Oligo ALOH-D1
                     /note="Oligo ALOH-D1: 5´-TCCCCGCGGACCGACATGGTGACGGGTGAG-3´"
     misc_feature    complement(1737..1760)
                     /label=Oligo ALOH-IgAP2
                     /note="Oligo ALOH-IgAP2"
     misc_feature    1761..1784
                     /label=Oligo NalP-ALOH3
                     /note="Oligo NalP-ALOH3"
     rep_origin      2693..3148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             3335..3994
                     /codon_start=1
                     /label=cat
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4326..4914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
       61 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg
     1201 ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca
     1261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     1321 caatttcaca caggaaacag ctatgaccat gattacgaat ttctagataa cgagggcaaa
     1381 tcatgaaata cctattgcct acggcagccg ctggattgtt attactcgcg gcccagccgg
     1441 ccatggcgca tcaccatcac catcacgcgg ccgctgcggg ggccgaattc gtcgacggtg
     1501 cgccggtgcc gtatccggat ccgctggaac cgatcgacaa ttcagccgca attagtatgg
     1561 caaatccacg tccaccaaca ccgcggacac ctacgccggg tccggatctg aatgtggata
     1621 atgacctgcg accggaggcg ggtagctaca ttgcgaacct tgcagcagcg aataccatgt
     1681 tcaccacgcg tctgcatgag cgtctgggta atacgtacta taccgacatg gtgacgggtg
     1741 agcagaaaca aaccactatg tggatgcgcc atgaaggtgg tcataataaa tggcgtgatg
     1801 gcagcggcca gctgaaaacc caaagcaatc gctatgttct gcaactggga ggcgatgtcg
     1861 cgcagtggag ccaaaacggc agcgaccgct ggcatgttgg ggtcatggcg ggatatggca
     1921 acagcgacag caaaaccatt tcctcgcgaa ccggttatcg tgcaaaagcg agtgtgaacg
     1981 gatatagcac aggcctctat gccacctggt atgccgatga cgagtcgcgt aatggcgcgt
     2041 atctcgacag ttgggcgcag tacagctggt ttgataacac agtgaaaggg gatgacttac
     2101 aaagtgaatc ctataaatca aaaggattta ccgcttcact ggaagctgga tacaaacaca
     2161 aattagctga atttaatggc agccagggaa cgcgtaatga atggtatgtt cagccgcaag
     2221 cacaggttac ctggatggga gtcaaagccg ataagcaccg cgaaagcaac ggaaccctcg
     2281 ttcatagcaa cggtgatggc aatgttcaaa cccgacttgg cgtaaaaacc tggctgaaga
     2341 gccaccataa aatggatgac ggtaaatccc gcgagttcca gccgtttgta gaagtgaact
     2401 ggctacataa cagtaaggat ttcagcacca gtatggatgg cgtgtctgtc actcaggatg
     2461 gagcccgaaa tattgctgag ataaaaaccg gggtggaagg acagctaaat gccaacctga
     2521 atgtctgggg gaatgtgggc gttcaggttg ccgatagggg atataatgac acctctgcaa
     2581 tggttggcat taagtggcaa ttctgaaagc ttgacctgtg aagtgaaaaa tggcgcacat
     2641 tgtgcgacat tttttttgtc tgccgtttac cgctactgcg tcacggatcc ccacgcgccc
     2701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2821 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     2881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3061 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3121 tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     3181 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gtcgagacgt
     3241 tgggtgaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     3301 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     3361 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     3421 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     3481 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     3541 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     3601 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     3661 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     3721 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     3781 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     3841 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     3901 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     3961 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     4021 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     4081 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
     4141 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     4201 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgctcga catgaccaaa
     4261 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4441 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4921 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catg
//
