LOCUS       Exported                3991 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 9, 2021 from SnapGene Viewer 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     185..208
                     /label=RSP-48
     CDS             343..1545
                     /codon_start=1
                     /label=Vamy-HlyA
                     /translation="MAHHHHHHAAQPAMAQVQLVESWGGSVQAGGSLRLSCTAPGFTSN
                     SCRMDWYRQAAGKQREWVSSISTDGRTSYADSVKGRFTISKDKAKDTVYLQMNSLKPED
                     TAIYYCAVRTNGYRPQSHEFRYWGPGTQVTVSSAAASGAASTYPYDVPDYALEVLFQGP
                     ASTPGGAPVPYPDPLEPAGENSLAKNVLSGGKGNDKLYGSEGADLLDGGEGNDLLKGGY
                     GNDIYRYLSGYGHHIIDDEGGKDDKLSLADIDFRDVAFKREGNDLIMYKAEGNVLSIGH
                     KNGITFKNWFEKESDDLSNHQIEQIFDKDGRVITPDSLKKAFEYQQSNNKVSYVYGHDA
                     STYGSQDNLNPLINEISKIISAAGNFDVKEERSAASLLQLSGNASDFSYGRNSITLTAS
                     A"
     misc_feature    complement(349..366)
                     /label=His tag
                     /note="His tag"
     misc_feature    382..753
                     /label=Vhh Amy
                     /note="Vhh Amy"
     misc_feature    781..807
                     /label=HA-tag
                     /note="HA-tag"
     misc_feature    808..831
                     /label=PreScission site
                     /note="PreScission site"
     misc_feature    847..885
                     /label=Etag
                     /note="Etag"
     misc_feature    892..1542
                     /label=HlyA
                     /note="HlyA"
     misc_feature    complement(2190..3050)
                     /label=AmpR
                     /note="AmpR"
     misc_feature    3151..3870
                     /label=Ori pUC
                     /note="Ori pUC"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aatttagatc
      241 tgaattcggg cccttcgaaa attaatacga ctcactatag ggagaccaca acggtttccc
      301 tctagaaata attttgttta actttaagaa ggagatatat tcatggctca tcaccatcac
      361 catcacgcgg cccagccggc catggctcag gtgcagctgg tggagtcttg gggaggctcg
      421 gtgcaggctg gggggtctct gagactctcc tgcacagccc ctggattcac ctccaatagc
      481 tgccgcatgg actggtaccg ccaggctgca gggaagcagc gcgagtgggt ctcatctatt
      541 agtactgatg gtcgcacaag ctatgcagac tccgtgaagg gccgattcac catctccaaa
      601 gacaaagcca aggacacggt gtatctgcaa atgaacagcc tgaaacctga ggacacggcc
      661 atctattact gtgccgtgag gacgaatggg tatcgtccgc aatctcacga atttcgctac
      721 tggggcccgg ggacccaggt caccgtctcc tcagcggccg catcgggggc cgcgtcgacg
      781 tacccatacg atgttccaga ttacgctctg gaagttctgt tccaggggcc cgcttcgacg
      841 cccgggggtg cgccggtgcc gtatccggat ccgctggaac cggccgggga aaattctctt
      901 gctaaaaatg tattatccgg tggaaaaggt aatgacaagt tgtacggcag tgagggagca
      961 gacctgcttg atggcggaga agggaatgat cttctgaaag gtggatatgg taatgatatt
     1021 tatcgttatc tttcaggata tggccatcat attattgacg atgaaggggg gaaagacgat
     1081 aaactcagtt tagctgatat agatttccgg gacgttgcct ttaagcgaga agggaatgac
     1141 ctcattatgt ataaagctga aggtaatgtt ctttctattg gccacaaaaa tggtattaca
     1201 tttaaaaact ggtttgaaaa agagtcagat gatctctcta atcatcagat agagcagatt
     1261 tttgataaag acggcagggt aatcacacca gattctctta aaaaagcatt tgaatatcag
     1321 cagagtaata acaaggtaag ttatgtgtat ggacatgatg catcaactta tgggagccag
     1381 gacaatctta atccattaat taatgaaatc agcaaaatca tttcagctgc aggtaacttc
     1441 gatgttaagg aggaaagatc tgccgcttct ttattgcagt tgtccggtaa tgccagtgat
     1501 ttttcatatg gacggaactc aataactttg acagcatcag cataatatat taatttaaat
     1561 gatagcaatc ttactgggct gtgccaagct tggcactggc cgtcgtttta caacgtcgtg
     1621 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1681 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1741 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1801 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1861 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1921 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1981 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2041 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3361 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3961 gcagcgagtc agtgagcgag gaagcggaag a
//
