LOCUS       Exported                6718 bp ds-DNA     circular SYN 30-APR-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        991..1009
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff"
     misc_feature    1062..2498
                     /label=HyPer7.2
                     /note="color: #ffcc00"
     misc_feature    1110..1112
                     /label=L17V
                     /note="color: #a6acb3"
     misc_feature    1185..1187
                     /label=M42V
                     /note="color: #a6acb3"
     misc_feature    2505..3608
                     /label=DAAO
                     /note="color: #a6acb3"
     CDS             3609..3629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3633..3653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3657..3677
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    3726..3860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(3973..3991)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4012..4028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    4036..4052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(4060..4090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    4105..4126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
                     /note="color: #31849b"
     rep_origin      complement(4414..5002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5173..6033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6034..6138)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(6164..6619)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        join(6697..6718,1..956)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian 
                     cytomegalovirusmajor immediate early transcription unit 
                     IE94"
                     /note="color: #ffffff"
ORIGIN
        1 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
       61 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
      121 ggcaccatgc caattcaata tggcggacct ggcactgtgc caactgggga ggggtctact
      181 tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat
      241 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
      301 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
      361 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
      421 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
      481 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga
      541 cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc
      601 ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc
      661 atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac
      721 atcaatatct attaatagta acttggcaag tacattacta ttggaagtac gccagggtac
      781 attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac
      841 atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa
      901 tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaaccgcc
      961 attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa tacaagctac
     1021 ttgttctttt tgcaggatcc catcgattcg aattcgccac catgcacctg gctaatgagg
     1081 agcaaaacga attggaaggt gcttttaagg tgggcttgat ctttaccgtc gccccgtact
     1141 tgctgcctaa gttgatcgtc agcctgcgcc gcaccgcccc gaaggtgcct ttgatgttgg
     1201 aggagaatta cacccacacc cttaccgagt cactgaaacg cggcgacgtt gacgccatca
     1261 ttgtggcaga gccgtttcaa gagccgggta ttgtgactga acctttgtat gacgagccgt
     1321 ttttcgttat cgttccgaag ggccatagct tcgaggagtt ggatgccgtt agccctcgca
     1381 tgttgggcga agaacaagtt ctgcttctga ccgagggcaa ctgtatgcgt gaccaggtgc
     1441 ttagctccgc cggcttcaac agcaacaacg tctatatcat ggccgacaag cagaagaacg
     1501 gcatcaaggc caacttcaag atccgccaca acgtcgagga cggcagcgtg cagctcgccg
     1561 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1621 acctgagctt ccagtccgtc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1681 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaacgtgg
     1741 atggcggtag cggtggcacc ggcagcaagg gcgaggagct gttcaccggg gtggtgccca
     1801 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1861 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     1921 ccgtgccctg gcccaccctc gtgaccaccc tcagctacgg cctgaagtgc ttcgcccgct
     1981 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2041 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2101 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcggcttc aaggaggacg
     2161 gcaacatcct ggggcacaag ctggagtaca acggccccag ttgcagcgag cttgccgcca
     2221 aacagcgcat tcagggtctc accaacaccc ttcaaggctc aagcatcaat accatccgcc
     2281 acatggttgc cagtggcttg gcaatcagcg tcttgcctgc cactgccctt accgaaaacg
     2341 accacatgct gtttagcatt atcccgtttg aaggcacccc gcctagccgc cgcgttgtcc
     2401 tggcctatcg ccgcaacttt gtccgcccta aggccctttc agccatgaaa gccgcaatta
     2461 tgcaaagcca actgcatggt gttagcttca tctgcgatgc tagcagtggt cacagccaga
     2521 agagggtggt cgtgctgggc tccggagtca tcgggctctc tggcgccctg attctcgcta
     2581 gaaaaggcta ctccgtgcat atcctggcca gagacttgcc tgaggatgtc agctcccaga
     2641 ccttcgcaag cccctgggcc ggagctaact ggacaccatt tatgaccctg acagacggac
     2701 ccagacaagc caagtgggaa gagtccacct tcaagaaatg ggtggagctg gttcccacag
     2761 gccacgctat gtggctgaaa gggaccagaa ggtttgccca gaatgaggat ggcctcctgg
     2821 gacattggta caaggacatc acccctaact ataggccact tccaccttct gaatgtccac
     2881 ctggagccat tggagtgaca tacgacaccc tgagcgtcca cgctcccaag tattgcagat
     2941 accttgccag agagctgcag aaacttgggg ctaccttcga aaggagaaca gtgactagcc
     3001 tggagcaagc cttcgatgga gctgacctcg tggtcaacgc caccgggctg ggagctaagt
     3061 ccattgccgg catcgatgac caggctgccg agcccattag gggacagacc gtgcttgtca
     3121 aatctccctg taagaggtgc acaatggata gctccgaccc tgcttctccc gcttatatca
     3181 ttcctagacc aggaggagaa gtgatctgtg gcggaaccta cggagtcggc gattgggacc
     3241 tgagcgtgaa tcccgaaaca gtccaaagga ttctcaagca ctgcctgaga cttgacccta
     3301 ccattagctc cgatggaact atcgagggca ttgaagtgct gagacataac gttggcctga
     3361 gacccgccag gagaggagga cctagggtgg aggctgaaag aatcgtcttg cccctggacc
     3421 ggaccaagag ccctctctct ctgggaagag ggtccgcacg ggctgcaatg gagaaggaag
     3481 tgacccttgt ccacgcctac ggctttagta gcgcagggta tcagcaatcc tggggagccg
     3541 tggaggacgt ggctcagctg gtcgatgaag ccttccaaag ataccacggc gcagctaggg
     3601 agagcgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa
     3661 aaaagaagag aaaggtataa aagcttgcct cgagcctcta gaactatagt gagtcgtatt
     3721 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3781 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3841 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3901 gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc
     3961 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     4021 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat
     4081 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4141 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     6181 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6241 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6301 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6361 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     6421 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     6481 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     6541 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     6601 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6661 tcggtgcggg cctcttcgct attacgccag tcgaccgcca attcaatatg gcgtatat
//
