LOCUS       Exported                6862 bp ds-DNA     circular SYN 30-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HyPer7.2DAAO-MITO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 30, 2021 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(572..1432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  572 .. 1363 / #ccffcc
                        2: 1364 .. 1432 / #ccffcc / signal sequence
                     Cleavage site after base 1363"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1433..1537)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(1563..2018)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2096..3073
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3108..3126
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3179..3364
                     /label=2xMTS
                     /note="color: #a6acb3"
     CDS             3179..3253
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 3253"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3275..3346
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 3346"
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    3372..4804
                     /label=HyPer7.2
                     /note="color: #ffcc00"
     misc_feature    4811..5911
                     /label=DAAO
                     /note="color: #00ccff"
     polyA_signal    5987..6121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(6234..6252)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6273..6289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6297..6313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6321..6351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6321 .. 6327 / #ffffff / -10
                        2: 6328 .. 6345 / #ffffff
                        3: 6346 .. 6351 / #ffffff / -35"
     protein_bind    6366..6387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(join(6675..6862,1..401))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
       61 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      241 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
      601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
      721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
      781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
      841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
      901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
      961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1561 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     1621 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     1681 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     1741 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     1801 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     1861 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     1921 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     1981 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     2041 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa
     2101 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa
     2161 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa
     2221 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacctggc actgtgccaa
     2281 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca
     2341 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag
     2401 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc
     2461 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt
     2521 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc
     2581 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat
     2641 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg
     2701 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga
     2761 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat
     2821 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg
     2881 gaagtacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg
     2941 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg
     3001 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc
     3061 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac
     3121 tatagaatac aagctacttg ttctttttgc aggatcccat cgattcgaat tcgccaccat
     3181 gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc ggctcccagt
     3241 gccgcgcgcc aagatccatt cgttggggga tctgtccgtc ctgacgccgc tgctgctgcg
     3301 gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg
     3361 ggataccggt cacctggcta atgaggagca aaacgaattg gaaggtgctt ttaagctggg
     3421 cttgatcttt accgtcgccc cgtacttgct gcctaagttg atcgtcagcc tgcgccgcac
     3481 cgccccgaag atgcctttga tgttggagga gaattacacc cacaccctta ccgagtcact
     3541 gaaacgcggc gacgttgacg ccatcattgt ggcagagccg tttcaagagc cgggtattgt
     3601 gactgaacct ttgtatgacg agccgttttt cgttatcgtt ccgaagggcc atagcttcga
     3661 ggagttggat gccgttagcc ctcgcatgtt gggcgaagaa caagttctgc ttctgaccga
     3721 gggcaactgt atgcgtgacc aggtgcttag ctccgccggc ttcaacagca acaacgtcta
     3781 tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacgt
     3841 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagcttccag tccgtcctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctgtaca acgtggatgg cggtagcggt ggcaccggca gcaagggcga
     4081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4201 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctcag
     4261 ctacggcctg aagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4441 gaagggcatc ggcttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgg
     4501 ccccagttgc agcgagcttg ccgccaaaca gcgcattcag ggtctcacca acacccttca
     4561 aggctcaagc atcaatacca tccgccacat ggttgccagt ggcttggcaa tcagcgtctt
     4621 gcctgccact gcccttaccg aaaacgacca catgctgttt agcattatcc cgtttgaagg
     4681 caccccgcct agccgccgcg ttgtcctggc ctatcgccgc aactttgtcc gccctaaggc
     4741 cctttcagcc atgaaagccg caattatgca aagccaactg catggtgtta gcttcatctg
     4801 cgatgctagc agtggtcaca gccagaagag ggtggtcgtg ctgggctccg gagtcatcgg
     4861 gctctctggc gccctgattc tcgctagaaa aggctactcc gtgcatatcc tggccagaga
     4921 cttgcctgag gatgtcagct cccagacctt cgcaagcccc tgggccggag ctaactggac
     4981 accatttatg accctgacag acggacccag acaagccaag tgggaagagt ccaccttcaa
     5041 gaaatgggtg gagctggttc ccacaggcca cgctatgtgg ctgaaaggga ccagaaggtt
     5101 tgcccagaat gaggatggcc tcctgggaca ttggtacaag gacatcaccc ctaactatag
     5161 gccacttcca ccttctgaat gtccacctgg agccattgga gtgacatacg acaccctgag
     5221 cgtccacgct cccaagtatt gcagatacct tgccagagag ctgcagaaac ttggggctac
     5281 cttcgaaagg agaacagtga ctagcctgga gcaagccttc gatggagctg acctcgtggt
     5341 caacgccacc gggctgggag ctaagtccat tgccggcatc gatgaccagg ctgccgagcc
     5401 cattagggga cagaccgtgc ttgtcaaatc tccctgtaag aggtgcacaa tggatagctc
     5461 cgaccctgct tctcccgctt atatcattcc tagaccagga ggagaagtga tctgtggcgg
     5521 aacctacgga gtcggcgatt gggacctgag cgtgaatccc gaaacagtcc aaaggattct
     5581 caagcactgc ctgagacttg accctaccat tagctccgat ggaactatcg agggcattga
     5641 agtgctgaga cataacgttg gcctgagacc cgccaggaga ggaggaccta gggtggaggc
     5701 tgaaagaatc gtcttgcccc tggaccggac caagagccct ctctctctgg gaagagggtc
     5761 cgcacgggct gcaatggaga aggaagtgac ccttgtccac gcctacggct ttagtagcgc
     5821 agggtatcag caatcctggg gagccgtgga ggacgtggct cagctggtcg atgaagcctt
     5881 ccaaagatac cacggcgcag ctagggagag cctgcccccc ctggagcggc tgaccctgta
     5941 aaagcttgcc tcgagcctct agaactatag tgagtcgtat tacgtagatc cagacatgat
     6001 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6061 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6121 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6181 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag
     6241 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6301 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
     6361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6841 ctcccttcgg gaagcgtggc gc
//
