LOCUS Exported 8636 bp DNA circular SYN 09-FEB-2022 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 8636) AUTHORS NYU School of Medicine TITLE Direct Submission JOURNAL Exported Feb 9, 2022 from SnapGene 6.0.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8636 /mol_type="other DNA" /organism="recombinant plasmid" primer_bind complement(1..47) /label=oSP218 /note="color: black; sequence: agtggatcccccgggctgcaggaattataacttcgtataatgtatgctatacgaagtta t" protein_bind 1..34 /label=loxM /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." /note="color: #00ff00; direction: RIGHT" primer_bind complement(1..33) /label=oJML0079 LandingPadPITCh-5b_Rev /note="color: black; sequence: TAACTTCGTATAAAGTATCCTATACGAAGTTATACGCG" primer_bind 1..31 /label=oSP55_EF1_Alpha_For_ins_413 /note="color: black; sequence: cggtatcgataagcttgatatcgACGCGTataacttcgtataggatactttatacgaag t" primer_bind 1..23 /label=oSP282_InsLoxP /note="color: black; sequence: ataacttcgtatagcatacattatacgaagttatTTataacttcgtataggatacttta " primer_bind 2..39 /label=oJML0079 LandingPadPITCh-5b_Rev /note="color: black; sequence: TAACTTCGTATAAAGTATCCTATACGAAGTTATACGCG" primer_bind complement(4..47) /label=oSP60_Cre_Rev_ins_413 /note="color: black; sequence: tagtggatcccccgggctgcaggACGCGTataacttcgtataatgtatgctatacgaag t" primer_bind complement(4..39) /label=oSP55_EF1_Alpha_For_ins_413 /note="color: black; sequence: cggtatcgataagcttgatatcgACGCGTataacttcgtataggatactttatacgaag t" primer_bind complement(5..34) /label=oJML0019 PIGALandingPad-1_Rev /note="color: black; sequence: TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA G" primer_bind 6..42 /label=oJML0082 LandingPadPITCh-3b_For /note="color: black; sequence: TTCGTATAGCATACATTATACGAAGTTATACGCGCCT" primer_bind complement(9..35) /label=oJML0253 Lvec-HTRA1-UL150-41homol_Rev /note="color: black; sequence: ATCCATTCTGCAGCTCATGCATCAAAGAGTAATTATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0223 Lvec-HTRA1-LIMS1homol_Rev /note="color: black; sequence: GTTGTGCTGACAGTCATAGCTAGATAGTCCAATCATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0239 Lvec-HTRA1-LIMS3homology_Rev /note="color: black; sequence: CTAATTTTCTACGATGGAGGCAATAAACAGACTCATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0250 Lvec-HTRA1-UL300-1homol_Rev /note="color: black; sequence: CCAAGGCTGCCAAGTGGCAGAGCCCAGATGTGAAATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0251 Lvec-HTRA1-UL250-14homol_Rev /note="color: black; sequence: TGGATTCCATCACCAGGGAGCTCAAGGTTCCAGAATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0252 Lvec-HTRA1-UL200-28homol_Rev /note="color: black; sequence: ACCATGCCCGGCTGAGTTTTATATCTTTTGTAGAATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0254 Lvec-HTRA1-UL100-54homol_Rev /note="color: black; sequence: GTACTCTTGATATCCTGTTGATGTGGTGGTCACAATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(9..34) /label=oJML0255 Lvec-HTRA1-UL50-68homol_Rev /note="color: black; sequence: GAATGGGAAGGAGAATCAATTTTCACTACTTACCATAACTTCGTATAAAGTATCCTATA C" primer_bind complement(12..78) /label=oSP89_correcting_CEGS_Frag1_Rev /note="color: black; sequence: CTGACGGGCACCGGAGCCataacttcgtataaagtatccta" primer_bind complement(36..55) /label=oRB_075 /note="color: black; sequence: ttacgtctgctgcaggcgcg" primer_bind 57..80 /label=oJML0145 EF1aProm-BsmBI_For /note="color: black; sequence: GTGGTGCGTCTCTTCGGGGCTCCGGTGCCCGTCAGTG" primer_bind 60..90 /label=oSP257_DeletionofloxM /note="color: black; sequence: aggtcgacggtatcgataagcttgatatcgGGCTCCGGTGCCCGTCAGTGGGCAGAGCG C" primer_bind 60..78 /label=oLM1204.PL2.F4 /note="color: black; sequence: CCCACCTTCTGGTCGATTGGGCTGGCGGGCTCCGGTGCCCGTCAG" promoter 61..1239 /standard_name="EF-1-alpha promoter" /label=EF-1-alpha promoter /note="Human EEF1A1 promoter" /note="color: #ffffff; direction: RIGHT" primer_bind 61..80 /label=oLM1273.marker.R /note="color: black; sequence: GGCTCCGGTGCCCGTCAGTG" primer_bind 61..80 /label=RB_065_GG /note="color: black; sequence: ATGCGGTCTCGCAGTGGCTCCGGTGCCCGTCAGTG" primer_bind 61..79 /label=oLM878.dl5.F /note="color: black; sequence: ATGGGGATTCTCGGGACCCCTCCTCTTCCTGGCTCCGGTGCCCGTCAGT" primer_bind 61..78 /label=oJML0049 HPRT1-PIGALPinsertionA_For /note="color: black; sequence: CATGAGTACCCAGAGTGGCTTCGGCTCCGGTGCCCGTCAG" primer_bind complement(90..107) /label=oJML0189 LP5confirm_Rev /note="color: black; sequence: GGGACTGTGGGCGATGTG" primer_bind complement(99..117) /label=oJML0236 LP-confirm5b_Rev /note="color: black; sequence: CAACTTCTCGGGGACTGTG" primer_bind complement(165..188) /label=oSP66_CEGS_MMEJ_Jnctn1R /note="color: black; sequence: ACGACATCACTTTCCCAGTTTACC" primer_bind complement(227..250) /label=oSP163_EF1Alpha_Rev_alt /note="color: black; sequence: CGGCGACTACTGCACTTATATACG" primer_bind complement(239..260) /label=oLM1281.RgBlock.F /note="color: black; sequence: AGAACGTTCACGGCGACTACTG" primer_bind complement(290..310) /label=oSP351_EF1alpha_250bp_NheI_R /note="color: black; sequence: GCTAGCaaccacacacggcacttacct" intron 292..1230 /standard_name="EF-1-alpha intron A" /label=EF-1-alpha intron A /note="color: #------" primer_bind complement(294..312) /label=oSP290_250bp2loxStuffer_EF1alpha_R /note="color: black; sequence: ctcgagataacttcgtataaagtatcctatacgaagttatCggaaccacacacggcact t" primer_bind complement(421..480) /label=oSP91_correcting_CEGS_Frag2_Rev /note="color: black; sequence: CCAGGCCTCAACTCAAGCACGAGGCGAAGGGGCTCCTTAAGCGCAAGGCCTCGAACTCT C" primer_bind complement(535..560) /label=oSP352_Ef1alpha_500bp_NheI_R /note="color: black; sequence: GCTAGCatggctagagacttatcgaaagcagc" primer_bind complement(535..560) /label=oSP291_500bp2loxStuffer_EF1alpha_R /note="color: black; sequence: taacttcgtataaagtatcctatacgaagttatCatggctagagacttatcgaaagcag c" primer_bind complement(608..627) /label=DT1084R /note="color: black; sequence: TTGGCCCGCATTTACAAGAC" primer_bind 735..754 /label=DT1043F /note="color: black; sequence: AATCGGACGGGGGTAGTCTC" primer_bind 836..854 /label=oLM787.FAP.3.2-4.F /note="color: black; sequence: CACCAGTTGCGTGAGCGGA" primer_bind 840..859 /label=oRB_122_pEF1a-qPCR-F /note="color: black; sequence: AGTTGCGTGAGCGGAAAGAT" primer_bind 936..957 /label=oLM770.FAP4.F /note="color: black; sequence: CACACAAAGGAAAAGGGCCTTT" primer_bind complement(969..988) /label=oRB_123_pEF1a-qPCR-R /note="color: black; sequence: TGGAGTCACATGAAGCGACG" primer_bind complement(1039..1062) /label=oSP353_Ef1alpha_1000bp_NheI_R /note="color: black; sequence: GCTAGCccccaacctaaagacgacgtact" primer_bind complement(1039..1062) /label=oSP292_1000bp2loxStuffer_EF1alpha_R /note="color: black; sequence: agataacttcgtataaagtatcctatacgaagttatCccccaacctaaagacgacgtac t" primer_bind complement(1063..1085) /label=oLM788.FAP.3.2-4.R /note="color: black; sequence: AACTCCATCGCATAAAACCCCTC" primer_bind 1094..1117 /label=oSP67_CEGS_MMEJ_Jnctn2F /note="color: black; sequence: ctgagtgggtggagactgaagtta" primer_bind 1192..1211 /label=pEF1a-SeqF2 /note="color: black; sequence: CCTCAGACAGTGGTTCAAAG" primer_bind complement(1215..1239) /label=oJML0257 EF1aProm_Rev /note="color: black; sequence: TCACGACACCTGAAATGGAAGAAAA" primer_bind 1217..1269 /label=oJML0246 mScarlet-EF1alphahomol_F /note="color: black; sequence: TTCTTCCATTTCAGGTGTCGTGAATGGTGAGCAAGGGCGAGGC" primer_bind complement(1219..1268) /label=oJML0243 EF1aProm-mMaroonhomol-Rev /note="color: black; sequence: CTCCTCGCCCTTGCTCACCATTCACGACACCTGAAATGGAAG" primer_bind complement(1219..1244) /label=oJML0146 EF1aProm-BsmBI_Rev /note="color: black; sequence: CACCACCGTCTCGGCTCTCACGACACCTGAAATGGAAG" primer_bind complement(1230..1253) /label=oLM1241.LP-LM.R1 /note="color: black; sequence: atgacgttctcagtgctatccatccatgggctctcacgacacc" primer_bind 1247..1268 /label=oSP210_mCherry_Ded1_F /note="color: black; sequence: CTGGGGTAACAGCGGTGGTTCAAACAACTCTTCTTGGTGGATGGTGAGCAAGGGCGAGG " primer_bind 1249..1268 /label=oLM952.750.gfp.3.F /note="color: black; sequence: cctgcatccatctagatctcgatggtgagcaagggcgagg" CDS 1250..1945 /codon_start=1 /product="https://www.fpbase.org/protein/mscarlet/Recoded to eliminate ScaI site " /label=mScarlet /note="color: #ff0000" /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH STGGMDELYK" primer_bind 1250..1268 /label=oSP198_mCherry_Pab1_F /note="color: black; sequence: GTCTTTCAAAAAGGAGCAAGAACAACAAACTGAGCAAGCTATGGTGAGCAAGGGCGAGG " primer_bind complement(1250..1268) /label=GFP-SeqR1 /note="color: black; sequence: TCCTCGCCCTTGCTCACCAT" primer_bind complement(1654..1683) /label=oJML0248 midmScarlet-noBbsI_R /note="color: black; sequence: TCCCAGCCCATTGTTTTCTTCTGCATTACG" primer_bind 1655..1684 /label=oJML0249 midmScarlet-noBbsI_F /note="color: black; sequence: GTAATGCAGAAGAAAACAATGGGCTGGGAA" primer_bind 1747..1760 /label=oJML0034 PIGALandingPad-1b_Rev /note="color: black; sequence: TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA GTTATACGCGCCTGCAGGACG" primer_bind 1747..1759 /label=oLM1247.LP-LM.R4 /note="color: black; sequence: GCGGCCGCataacttcgtataggatactttatacgaagttatgaacgcgcctgcaggac " primer_bind complement(1925..1945) /label=oSP199_mCherry_R /note="color: black; sequence: TTACTTGTACAGCTCGTCCATGCC" primer_bind complement(1925..1945) /label=oSP354_mCherry_NheI_R /note="color: black; sequence: GCTAGCttacttgtacagctcgtccatgcc" primer_bind complement(1925..1945) /label=oSP148_mCherry_Gly_Ser_R /note="color: black; sequence: ACCACTAGCCTTACCAGCACTACCAGCACTCTTGTACAGCTCGTCCATGCC" primer_bind complement(1925..1945) /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R /note="color: black; sequence: CAGATGGCTGGCAACTAGAAGGCACAGTCGttacttgtacagctcgtccatgcc" primer_bind complement(1925..1945) /label=oSP544_GFP_Lenti_R /note="color: black; sequence: acaaattttgtaatccagaggttgattaggatctatttacttgtacagctcgtccatgc c" primer_bind complement(1925..1945) /label=oSP295_2000bp2loxStuffer_mCherry_R /note="color: black; sequence: gataacttcgtataaagtatcctatacgaagttatCttacttgtacagctcgtccatgc c" primer_bind complement(1925..1945) /label=oSP224_2lox_LHM21_Rev /note="color: black; sequence: ctgcagcatatcatggcgtgtaatatgagagacgccttacttgtacagctcgtccatgc c" primer_bind complement(1926..1953) /label=oJML0247 mScarlet-P2A8homol_R /note="color: black; sequence: CCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCCTTGTACAGCTCGTCCATGC" primer_bind complement(1926..1947) /label=RB_066_GG /note="color: black; sequence: GCATGGTCTCTTGCCCTTGTACAGCTCGTCCATGC" primer_bind complement(1926..1945) /label=oJML0174 /note="color: black; sequence: GTGGTGGAAGACTCAGTACTACTTGTACAGCTCGTCCATGC" primer_bind complement(1926..1945) /label=oJML0066 /note="color: black; sequence: GTGGTGGCGGCCGCCTACTTGTACAGCTCGTCCATGC" primer_bind 1927..1973 /label=oRB_128_P2A-F-XbaI /note="color: black; sequence: catggacgagctgtacaagtctagaggctcaggcgccaccaacttta" primer_bind 1937..1965 /label=oLM1244.LP-LM.F3 /note="color: black; sequence: ccgccaccacctgttccagggatccggaagcggtgccac" primer_bind 1945..1971 /label=oRB_204-P2A-F-BamHI /note="color: black; sequence: ACTGGGATCCGGAAGCGGTGCCACCAACTT" primer_bind 1946..1978 /label=oRB_126_P2A-F-BamHI /note="color: black; sequence: cgatggatccggaagcggtgccaccaactttagcttg" CDS 1952..2017 /codon_start=1 /product="2A peptide from porcine teschovirus-1, recoded by Jon Laurent" /label=P2A3 /note="2A peptide from porcine teschovirus-1, recoded" /note="color: #993366" /translation="GSGATNFSLLKQAGDVEENPGP" primer_bind 1982..2045 /label=oJML0245 Cre-P2Amut1homol_F /note="color: black; sequence: TCAAGCAGGCTGGCGACGTCGAGGAGAATCCCGGGCCTATGGCCAATTTACTGACCGTA C" primer_bind 1982..2045 /label=oJML0130 /note="color: black; sequence: TGAAGCAGGCCGGCGACGTGGAGGAGAACCCCGGCCCCATGGCCAATTTACTGACCGTA C" primer_bind 2022..2043 /label=DT1023F /note="color: black; sequence: tcatggccaatttactgaccgt" primer_bind 2023..2045 /label=oLM5.NotI-CRE-EBD-tt-F /note="color: black; sequence: GATCGCGGCCGCATGTCCAATTTACTGACCGTAC" primer_bind 2023..2042 /label=oLM25.413-CRE-F /note="color: black; sequence: gacggtatcgataagcttgatatcgaattcctgcagcccATGTCCAATTTACTGACCG" CDS 2024..3052 /codon_start=1 /product="site-specific recombinase. Recoded to eliminate BamHI site." /label=Cre /note="color: #cc99ff" /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" primer_bind 2027..2050 /label=oJML0264 Cre-ATGdelete-EcoRI_F /note="color: black; sequence: GTGGTGGAATTCGCCAATTTACTGACCGTACACCAA" primer_bind 2028..2048 /label=LM118.CRE-EBD.BsaI.F /note="color: black; sequence: ggtctcaaATGTCCAATTTACTGACCGTACACC" primer_bind complement(2154..2172) /label=oSP78_CEGS_MMEJ_Jnctn7R /note="color: black; sequence: CGGCAAACGGACAGAAGCA" primer_bind 2208..2232 /label=oLM886.Cre.F1 /note="color: black; sequence: AATGGTTTCCCGCAGAACCTGAAGA" primer_bind 2451..2470 /label=DT1096F /note="color: black; sequence: ACCAGGTTCGTTCACTCATG" primer_bind complement(2504..2524) /label=oLM887.Cre.R1 /note="color: black; sequence: AGCAATCCCCAGAAATGCCAG" primer_bind 2689..2719 /label=oJML0140 /note="color: black; sequence: GCGATGGATTTCCGTGTCTGGTGTAGCTGAT" primer_bind complement(2689..2719) /label=oJML0141 /note="color: black; sequence: ATCAGCTACACCAGACACGGAAATCCATCGC" primer_bind complement(2690..2720) /label=oJML0062 /note="color: black; sequence: CATCAGCTACACCAGACACGGAAATCCATCG" primer_bind 2690..2720 /label=oJML0063 /note="color: black; sequence: CGATGGATTTCCGTGTCTGGTGTAGCTGATG" primer_bind 2831..2854 /label=oLM888.Cre.F2 /note="color: black; sequence: CGATTGATTTACGGCGCTAAGGAT" primer_bind 2924..2943 /label=DT1046F /note="color: black; sequence: CGCGCTGGAGTTTCAATACC" primer_bind complement(3029..3049) /label=oLM889.Cre.R2 /note="color: black; sequence: GCCATCTTCCAGCAGGCGCAC" primer_bind 3052..3071 /label=oLM852.cEBD-FKH1.F_ /note="color: black; sequence: AACGTGAACAATTCCTCTCTGAGTCTCGAGCCATCCGCTGGAG" primer_bind 3052..3071 /label=oLm848.cEBD-WHI5.F /note="color: black; sequence: GGATACCGAAGTGGAGACGTCTCTCGAGCCATCCGCTGGAG" CDS 3065..4003 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997). Recoded to eliminate XbaI site." /label=ERT2 /note="color: #99ccff" /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPATA" primer_bind complement(3373..3402) /label=oJML0131 /note="color: black; sequence: GGAGCGCCAGACGAGGCCAATCATCAGGATC" primer_bind 3376..3400 /label=LM120.QC.CRE-EBD.F /note="color: black; sequence: CCTGATGATTGGaCTCGTCTGGCGC" primer_bind complement(3376..3400) /label=LM121.QC.CRE-EBD.R /note="color: black; sequence: GCGCCAGACGAGtCCAATCATCAGG" primer_bind 3824..3845 /label=oSP560_ERT2_F /note="color: black; sequence: ctctatgacctgctgctggagg" primer_bind complement(3889..3948) /label=oJML0133 /note="color: black; sequence: TGCGATGAAGTAGAGCCCGCAGTGGCCAAGTGGCTTTGGTCCGTTTCCTCCACGGATGC C" primer_bind complement(3932..3991) /label=oJML0134 /note="color: black; sequence: GAAACCCTCTGCCTCCCCCGTGATGTAATACTTTTGCAAGGAATGCGATGAAGTAGAGC C" primer_bind 3975..4004 /label=oJML0135 /note="color: black; sequence: GGGAGGCAGAGGGTTTCCCTGCCACAGCTTGATGAAGATGAGGCCAACCTTCTATCAGA G" primer_bind complement(3977..4010) /label=oRB_127_ERT2-R-XbaI /note="color: black; sequence: tagctctagaagctgtggcagggaaaccctctgcctc" primer_bind complement(3983..4001) /label=oLM34.EBD-tag_R /note="color: black; sequence: atagcccgcatagtcaggaacatcgtatgggtaaaagatGACTGTGGCAGGGAAACCCT C" primer_bind complement(3987..4017) /label=DT1097R /note="color: black; sequence: cctgagcctctagaagctgtggcagggaaac" primer_bind complement(3990..4049) /label=DT1024R /note="color: black; sequence: ctgcctgcttcagcaggctaaagttggtggcgcctgagccTCTAGAAGCTGTGGCAGGG A" primer_bind 3998..4017 /label=DT1098F /note="color: black; sequence: acagcttctagaggctcagg" primer_bind 4004..4031 /label=oRB_128_P2A-F-XbaI /note="color: black; sequence: catggacgagctgtacaagtctagaggctcaggcgccaccaacttta" CDS 4010..4075 /codon_start=1 /product="2A peptide from porcine teschovirus-1, recoded by Jon Laurent" /label=P2A1 /note="color: #993366" /translation="GSGATNFSLLKQAGDVEENPGP" primer_bind 4016..4029 /label=oRB_204-P2A-F-BamHI /note="color: black; sequence: ACTGGGATCCGGAAGCGGTGCCACCAACTT" primer_bind complement(4033..4049) /label=oJML0194 /note="color: black; sequence: CTGCCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCGGCACCGGGCTTGCGGGT" primer_bind 4039..4072 /label=oSP555_Puro_iCasp9_F /note="color: black; sequence: gaagcaggcaggcgacgtcgaggagaaccccggc" primer_bind 4076..4102 /label=oJML0260 Puro-SalI_F /note="color: black; sequence: GTGTGGTCGACATGACCGAGTACAAGCCCACG" primer_bind 4081..4102 /label=oJML0259 Puro-BamHI_F /note="color: black; sequence: GTGTGGGATCCATGACCGAGTACAAGCCCACG" primer_bind 4081..4101 /label=oJML0126 /note="color: black; sequence: GTGGTGGAAGACTCGAGCATGACCGAGTACAAGCCCAC" CDS 4082..4678 /codon_start=1 /standard_name="PuroR" /product="Streptomyces alboniger Puromycin N-acetyltransferase" /label=PuroR /note="color: #0000ff" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 4082..4102 /label=oJML0258 Puro-NcoI_F /note="color: black; sequence: GTGTGCCATGGATGACCGAGTACAAGCCCACG" primer_bind 4082..4102 /label=oLM854.puro.F /note="color: black; sequence: ATGACCGAGTACAAGCCCACG" primer_bind complement(4223..4246) /label=oSP561_Puro_R /note="color: black; sequence: cgtgaggaagagttcttgcagctc" primer_bind 4257..4275 /label=oLM487.puro.seq.F2 /note="color: black; sequence: TCGACATCGGCAAGGTGTG" primer_bind complement(4366..4392) /label=DT1101R /note="color: black; sequence: gccagccggGaaccgctcaactcggcc" primer_bind 4372..4398 /label=DT1100F /note="color: black; sequence: gttgagcggttCccggctggccgcgca" primer_bind complement(4393..4412) /label=oLM854.puro.R /note="color: black; sequence: CTTCCATCTGTTGCTGCGCG" primer_bind complement(4567..4577) /label=oJML0083 LandingPadPITCh-3b_Rev /note="color: black; sequence: TTTTGTGATGCTCGTCAGGGGGGCGGAG" primer_bind complement(4661..4716) /label=oJML0194 /note="color: black; sequence: CTGCCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCGGCACCGGGCTTGCGGGT" primer_bind complement(4661..4679) /label=oJML0127 /note="color: black; sequence: CAGCCTGGTCGAACGCAGACGCGGCACCGGGCTTGCGGGT" primer_bind 4661..4678 /label=oLM1246.LP-LM.F4 /note="color: black; sequence: acccgcaagcccggtgccTGAggtacctgaggccaaccttc" primer_bind 4677..4698 /label=oRB_204-P2A-F-BamHI /note="color: black; sequence: ACTGGGATCCGGAAGCGGTGCCACCAACTT" primer_bind 4677..4692 /label=oLM1244.LP-LM.F3 /note="color: black; sequence: ccgccaccacctgttccagggatccggaagcggtgccac" CDS 4679..4744 /codon_start=1 /product="2A peptide from porcine teschovirus-1" /label=P2A /note="2A peptide from porcine teschovirus-1" /note="color: #993366" /translation="GSGATNFSLLKQAGDVEENPGP" primer_bind complement(4679..4738) /label=oJML0138 /note="color: black; sequence: GGGGTTCTCCTCCACGTCGCCGGCCTGCTTCAGCAGGCTGAAGTTGGTGGCGCCGCTGC C" primer_bind 4685..4744 /label=oJML0139 /note="color: black; sequence: GGCGCCACCAACTTCAGCCTGCTGAAGCAGGCCGGCGACGTGGAGGAGAACCCCGGCCC C" primer_bind 4707..4740 /label=oJML0130 /note="color: black; sequence: TGAAGCAGGCCGGCGACGTGGAGGAGAACCCCGGCCCCATGGCCAATTTACTGACCGTA C" CDS 4745..6193 /codon_start=1 /product="Human mini PIGA" /label=hmPIGA /note="color: #00ffff" /translation="ACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNM GGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATT LFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSS VLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRH DSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHD RVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLP ENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDR VSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSIIDVAIDATG PRGAWTNNYSHSKRGGENNEISETR" primer_bind 4917..4949 /label=oSP360_oWZ058_F /note="color: black; sequence: GCCTGATTGAAAGAGGGCATAAGGTTATAATTG" primer_bind complement(5018..5043) /label=oSP48_PIGA_gRNA_2-2_HA2_For_w/o_homology /note="color: black; sequence: gactggttgtacatgactttcagagg" primer_bind 5028..5060 /label=oSP125_2-2_PCR1_For /note="color: black; sequence: TCATGTACAACCAGTCTACAGCCACGACCCTCTCCTGCAGGtttccataggctccgccc c" primer_bind 5028..5052 /label=oSP126_2-2_PCR2_For /note="color: black; sequence: atacattatacgaagttatACGCGCCTGCATGAATTCATGTACAACCAGTCTACAGCCA C" primer_bind 5041..5060 /label=oSP87_PIGA_gRNA_2-2_R /note="color: black; sequence: TTCTAGCTCTAAAACGTCTACAGCCACGACCCTCT" primer_bind complement(5041..5060) /label=oSP86_PIGA_gRNA_2-2_F /note="color: black; sequence: TAATACGACTCACTATAGAGAGGGTCGTGGCTGTAGAC" primer_bind 5044..5066 /label=oSP51_PIGA_gRNA_2-2_HA1_Rev_w/o_homology /note="color: black; sequence: tacagccacgaccctctttcaca" primer_bind complement(5164..5182) /label=oSP107_gRNA_2-2_R /note="color: black; sequence: AAGCCCCATTGTCTTGGCG" primer_bind 5213..5234 /label=oSP162_2-5_For_alt /note="color: black; sequence: GGATTTGCTGATGTCAGCTCGG" primer_bind 5216..5235 /label=oRB_150_hPIGA_F /note="color: black; sequence: TTTGCTGATGTCAGCTCGGT" primer_bind complement(5320..5344) /label=oSP50_PIGA_gRNA_2-5_HA2_For_w/o_homology /note="color: black; sequence: ttcaggattcagtgctgctcttagt" primer_bind 5329..5361 /label=oSP122_2-5_PCR1_For /note="color: black; sequence: AGCACTGAATCCTGAAATAGTGTCCGTCATTCCCCTGCAGGtttccataggctccgccc c" primer_bind 5329..5353 /label=oSP124_2-5_PCR2_For /note="color: black; sequence: atacattatacgaagttatACGCGCCTGCATGAATAGCACTGAATCCTGAAATAGTGTC C" primer_bind complement(5342..5363) /label=oSP84_PIGA_gRNA_2-5_F /note="color: black; sequence: TAATACGACTCACTATAGGAATGACGGACACTATTTC" primer_bind 5342..5361 /label=oSP85_PIGA_gRNA_2-5_R /note="color: black; sequence: TTCTAGCTCTAAAACGAAATAGTGTCCGTCATTCC" primer_bind 5345..5370 /label=oSP53_PIGA_gRNA_2-5_HA1_Rev_w/o_homology /note="color: black; sequence: atagtgtccgtcattcctaatgctgt" primer_bind complement(5390..5409) /label=oSP361_JB17709_R /note="color: black; sequence: TGCCTTCTAAATGGGTCTGG" primer_bind complement(5584..5603) /label=oRB_151_hPIGA_R /note="color: black; sequence: CCAAAAGACGCACCCTGTCA" primer_bind complement(6161..6202) /label=oRB_129_PIGA-R-KpnI /note="color: black; sequence: acgtggtaccttacctggtttcagatatctcattattctcaccccc" primer_bind complement(6164..6195) /label=oJML0006 /note="color: black; sequence: GCAACTAGAAGGCACAGTCGCTACCTGGTTTCAGATATCTCATTATTCTCACC" primer_bind complement(6169..6194) /label=oJML0261 PIGA-XbaI_R /note="color: black; sequence: GTGTGTCTAGACCTGGTTTCAGATATCTCATTATTC" primer_bind 6196..6216 /label=oLM1246.LP-LM.F4 /note="color: black; sequence: acccgcaagcccggtgccTGAggtacctgaggccaaccttc" polyA_signal 6203..6608 /label=hEIF1pA /note="Human EIF1 poly adenylation signal" /note="color: #a6acb3" primer_bind 6203..6224 /label=oJML0135 /note="color: black; sequence: GGGAGGCAGAGGGTTTCCCTGCCACAGCTTGATGAAGATGAGGCCAACCTTCTATCAGA G" primer_bind 6203..6224 /label=oJML0175 /note="color: black; sequence: GTGGTGGAAGACTCTACTTGAGGCCAACCTTCTATCAGAG" primer_bind complement(6247..6275) /label=oSP563_EF1AlphapA_R /note="color: black; sequence: ctacatgactgatggatcagtttgagatt" primer_bind 6508..6526 /label=oJML0190 /note="color: black; sequence: ACCCACAGCTTCTCAACGG" primer_bind complement(6578..6612) /label=oJML0136 /note="color: black; sequence: GTGGTGGAAGACTCTGAATGTCTCAAAAAACAAACGAACAAAAAACCAG" primer_bind complement(6625..6652) /label=oJML0064 CreTU-loxPhomol_Rev /note="color: black; sequence: CCGAGTGAGAGACACAAAAAATTCCAAC" primer_bind complement(6655..6706) /label=oJML0034 PIGALandingPad-1b_Rev /note="color: black; sequence: TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA GTTATACGCGCCTGCAGGACG" primer_bind complement(6656..6706) /label=oLM1247.LP-LM.R4 /note="color: black; sequence: GCGGCCGCataacttcgtataggatactttatacgaagttatgaacgcgcctgcaggac " primer_bind 6659..6703 /label=oSP60_Cre_Rev_ins_413 /note="color: black; sequence: tagtggatcccccgggctgcaggACGCGTataacttcgtataatgtatgctatacgaag t" primer_bind 6659..6669 /label=oRB_075 /note="color: black; sequence: ttacgtctgctgcaggcgcg" primer_bind 6670..6691 /label=oLM731.FAP.3HPRT.F /note="color: black; sequence: AATCGCCTTGCAGCACATCCCCCTTTCGCGAGCTCGAATTCATAACTTCGTATAGCATA C" primer_bind 6672..6698 /label=oJML0224 Rvec-HTRA1-LIMS1-34homol_For /note="color: black; sequence: ACCTCCTAGGCTCAAGCGATCTCTTGCTTTAGCCATAACTTCGTATAGCATACATTATA C" primer_bind 6673..6707 /label=oSP282_InsLoxP /note="color: black; sequence: ataacttcgtatagcatacattatacgaagttatTTataacttcgtataggatacttta " protein_bind 6673..6706 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #ff0000; direction: RIGHT" primer_bind 6673..6706 /label=oSP218 /note="color: black; sequence: agtggatcccccgggctgcaggaattataacttcgtataatgtatgctatacgaagtta t" primer_bind complement(6673..6706) /label=oSP218 /note="color: black; sequence: agtggatcccccgggctgcaggaattataacttcgtataatgtatgctatacgaagtta t" primer_bind 6673..6703 /label=oSP340 /note="color: black; sequence: cgaaggatctggaggccaccatggtgtcgataacttcgtatagcatacattatacgaag t" primer_bind 6673..6703 /label=oSP55_EF1_Alpha_For_ins_413 /note="color: black; sequence: cggtatcgataagcttgatatcgACGCGTataacttcgtataggatactttatacgaag t" primer_bind 6673..6698 /label=oJML0228 Rvec-HTRA1-LIMS1-18homol_For /note="color: black; sequence: TTTTCTGCTTCTCACTGCTGAGCAGACACATGTGATAACTTCGTATAGCATACATTATA C" primer_bind 6673..6698 /label=oJML0256 Rvec-HTRA1-UL80homol_For /note="color: black; sequence: GGGAGAAAGCACCTTCCCAGGATGCGTTGTATTTATAACTTCGTATAGCATACATTATA C" primer_bind complement(6676..6706) /label=oSP60_Cre_Rev_ins_413 /note="color: black; sequence: tagtggatcccccgggctgcaggACGCGTataacttcgtataatgtatgctatacgaag t" primer_bind complement(6677..6706) /label=oJML0019 PIGALandingPad-1_Rev /note="color: black; sequence: TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA G" primer_bind 6678..6706 /label=oJML0082 LandingPadPITCh-3b_For /note="color: black; sequence: TTCGTATAGCATACATTATACGAAGTTATACGCGCCT" primer_bind 6679..6706 /label=oSP83_CEGS_ori_Sbf1_For /note="color: black; sequence: tcgtatagcatacattatacgaagttatACGCGCCTGCAGGtttccataggctccgccc c" primer_bind 6684..6706 /label=oSP81_CEGS_Sbf1_For /note="color: black; sequence: tagcatacattatacgaagttatACGCGCCTGCAGGcttggtctgacagttaccaatgc t" primer_bind 6688..6706 /label=oSP124_2-5_PCR2_For /note="color: black; sequence: atacattatacgaagttatACGCGCCTGCATGAATAGCACTGAATCCTGAAATAGTGTC C" primer_bind 6688..6706 /label=oSP126_2-2_PCR2_For /note="color: black; sequence: atacattatacgaagttatACGCGCCTGCATGAATTCATGTACAACCAGTCTACAGCCA C" primer_bind complement(6691..6707) /label=oJML0188 LP_Rev /note="color: black; sequence: CTCAGGCAATAACTTCGTATAATG" terminator 6747..6840 /standard_name="AmpR Terminator" /label=Ter /note="color: #c0c0c0" primer_bind complement(6753..6786) /label=oJML0269 LPv4-Seq_R /note="color: black; sequence: TAATTTAAAAGGATCTAGGTGAAGATCCTTTTTG" primer_bind complement(6823..6850) /label=oJML0270 LPv4-Seq_Rb /note="color: black; sequence: GCATTGGTAACTGTCAGACCAAGTTTAC" primer_bind 6828..6851 /label=oSP81_CEGS_Sbf1_For /note="color: black; sequence: tagcatacattatacgaagttatACGCGCCTGCAGGcttggtctgacagttaccaatgc t" CDS complement(6841..7701) /standard_name="AmpR" /product="Beta-lactamase" /label=AmpR /note="color: #000000; direction: LEFT" primer_bind 6965..7005 /label=LM129.QC.Amp.F /note="color: black; sequence: gctgcaatgataccgcgagatccacgctcaccggctccaga" primer_bind 6968..7007 /label=oJML0067 midAmpR-noBsaI_For /note="color: black; sequence: GCAATGATACCGCGAGATCCACGCTCACCGGCTCCAGATT" primer_bind 7035..7045 /label=oSP257_DeletionofloxM /note="color: black; sequence: aggtcgacggtatcgataagcttgatatcgGGCTCCGGTGCCCGTCAGTGGGCAGAGCG C" primer_bind 7615..7674 /label=oJML0029 pCEP-Puro_PIGA-TU-1_Rev /note="color: black; sequence: CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAA G" primer_bind complement(7615..7674) /label=oJML0030 pCEP-Puro_PIGA-TU-2_For /note="color: black; sequence: CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGT G" promoter complement(7702..7806) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" primer_bind 7738..7759 /label=oLM486.puro.seq.F1 /note="color: black; sequence: gggttattgtctcatgagcgga" primer_bind 7790..7809 /label=DT425F pRS-origin /note="color: black; sequence: CAAATAGGGGTTCCGCGCAC" primer_bind complement(7813..7832) /label=oJML0044 PIGA-LPv3NoHIS_Rev /note="color: black; sequence: CACACCGCATAGATCCGTCGAGTTCAAGAGGAAAAGGACCCAGGTGGCACTTTTCGGGG A" primer_bind 7814..7832 /label=oLM862.SMCF.rep.R /note="color: black; sequence: CTTCCGTTGCCCCAGTAGCATCTCTGTCTGGTGACCTTGAccccgaaaagtgccacctg " primer_bind 7814..7832 /label=oLM447.BACconvert.F /note="color: black; sequence: catatcaccagctcaccgtctttcattgccatacggaattcCCCCGAAAAGTGCCACCT G" rep_origin 7909..8497 /direction=RIGHT /note="color: #ffcc00" primer_bind complement(8197..8216) /label=oJML0045 pMB1ori-seq_For /note="color: black; sequence: GCTGCGCCTTATCCGGTAAC" primer_bind 8197..8216 /label=oJML0046 pMB1ori-seq_Rev /note="color: black; sequence: GTTACCGGATAAGGCGCAGC" primer_bind 8361..8379 /label=oSP164_Bacparts_Rev_alt /note="color: black; sequence: gggtcggaacaggagagcg" primer_bind 8400..8423 /label=oJML0234 LPseq_Rev /note="color: black; sequence: GAAACGCCTGGTATCTTTATAGTC" primer_bind complement(8408..8467) /label=oJML0018 PIGALandingPad-1_For /note="color: black; sequence: TCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC A" primer_bind 8408..8467 /label=oJML0027 PIGALandingPad-5_Rev /note="color: black; sequence: TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTG A" primer_bind 8418..8438 /label=oLM437.H4.R /note="color: black; sequence: ATAGTCCTGTCGGGTTTCGCC" primer_bind 8460..8487 /label=oJML0083 LandingPadPITCh-3b_Rev /note="color: black; sequence: TTTTGTGATGCTCGTCAGGGGGGCGGAG" primer_bind complement(8473..8497) /label=oJML0053 Ori_For /note="color: black; sequence: TTTCCATAGGCTCCGCCCCCCTGAC" primer_bind complement(8479..8497) /label=oSP125_2-2_PCR1_For /note="color: black; sequence: TCATGTACAACCAGTCTACAGCCACGACCCTCTCCTGCAGGtttccataggctccgccc c" primer_bind complement(8479..8497) /label=oSP122_2-5_PCR1_For /note="color: black; sequence: AGCACTGAATCCTGAAATAGTGTCCGTCATTCCCCTGCAGGtttccataggctccgccc c" primer_bind complement(8479..8497) /label=oSP83_CEGS_ori_Sbf1_For /note="color: black; sequence: tcgtatagcatacattatacgaagttatACGCGCCTGCAGGtttccataggctccgccc c" primer_bind 8487..8506 /label=oJML0015 pUCori_SeqF /note="color: black; sequence: GCCTATGGAAAAACGCCAGC" primer_bind 8538..8557 /label=oJML0268 LPv4-Seq_F /note="color: black; sequence: GCTGGCCTTTTGCTCACATG" primer_bind join(8620..8636,1..14) /label=oJML0169 HPRT-PITCh-LP_For /note="color: black; sequence: TACGCGTACGTGTTTGGGCCATGAAGCAACGCGCGCCTCTCAGGTGATAACTTCGTATA G" primer_bind join(8635..8636,1..31) /label=oSP340 /note="color: black; sequence: cgaaggatctggaggccaccatggtgtcgataacttcgtatagcatacattatacgaag t" primer_bind complement(join(8636,1..33)) /label=oSP291_500bp2loxStuffer_EF1alpha_R /note="color: black; sequence: taacttcgtataaagtatcctatacgaagttatCatggctagagacttatcgaaagcag c" primer_bind join(8636,1..15) /label=oJML0187 LP_For /note="color: black; sequence: CTCAGGTGATAACTTCGTATAGG" ORIGIN 1 ataacttcgt ataggatact ttatacgaag ttatacgcgc ctgcagcaga cgtaagtcgg 61 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 121 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 361 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 421 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 481 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 541 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 601 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 661 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 721 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 781 agtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 841 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 901 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 961 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 1021 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1081 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1141 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1201 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agcccatgga tggtgagcaa 1261 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa 1321 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 1381 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc 1441 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta 1501 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 1561 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa 1621 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaaa caatgggctg 1681 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat 1741 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc 1801 caagaagccc gtgcagatgc ccggcgccta caacgttgac cgcaagttgg acatcacctc 1861 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac 1921 cggcggcatg gacgagctgt acaagggatc cggaagcggt gccaccaact ttagcttgct 1981 taagcaggcg ggcgacgtgg aggagaatcc cgggccggaa ttcatggcca atttactgac 2041 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa 2101 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct 2161 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc 2221 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa 2281 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg 2341 accaagtgac agcaatgctg tttcactggt tatgcggcgc atccgaaaag aaaacgttga 2401 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg 2461 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat 2521 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc 2581 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac 2641 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc 2701 cgtgtctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg 2761 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga 2821 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg 2881 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat 2941 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg 3001 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc 3061 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc 3121 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga 3181 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc 3241 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc 3301 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttcttgaatg 3361 tgcctggcta gagatcctga tgattggcct cgtctggcgc tctatggagc acccagtgaa 3421 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat 3481 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca 3541 aggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt 3601 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa 3661 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc aacagcagca 3721 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg 3781 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct 3841 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga 3901 ggaaacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcagaa 3961 gtattacatc acgggggagg cagagggttt ccctgccaca gcttctagag gctcaggcgc 4021 caccaacttt agcctgctga agcaggcagg cgacgtcgag gagaaccccg gccctgtcga 4081 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 4141 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 4201 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4261 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4321 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 4381 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 4441 gcccgcgtgg ttcctggcca ccgtcggcgt gtcgcccgac caccagggca agggtctggg 4501 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 4561 ggaaacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 4621 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg 4681 cagcggcgcc accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg 4741 gcccgcctgt agaggaggag ctgggaatgg ccaccgtgcc tcagctacac tctctcgggt 4801 tagccctgga agtctttaca catgtagaac ccgtacccat aatatatgca tggtatctga 4861 ctttttctac ccaaatatgg gaggcgtgga aagccacatt taccagctct ctcagtgcct 4921 gattgaaaga gggcataagg ttataattgt cacccatgct tatggaaatc gaaaaggcat 4981 ccgttacctc accagtggcc tcaaagtcta ttacttgcct ctgaaagtca tgtacaacca 5041 gtctacagcc acgaccctct ttcacagtct gccattgctc aggtacatat ttgttcggga 5101 gagagtcacg ataatccatt cacatagttc tttttctgct atggcccatg atgctctctt 5161 ccacgccaag acaatggggc ttcagacagt gttcacggac cattcccttt ttggatttgc 5221 tgatgtcagc tcggtgctta caaacaagct tctaaccgtg tctctttgtg atacaaacca 5281 catcatttgt gtgtcttata ctagtaagga aaatactgta ctaagagcag cactgaatcc 5341 tgaaatagtg tccgtcattc ctaatgctgt agatcctact gacttcactc cagacccatt 5401 tagaaggcat gatagtataa ctattgttgt tgtcagcaga cttgtttaca gaaaagggat 5461 cgatttgctt agtggtataa tacctgaact ctgtcagaaa tatccagatt taaatttcat 5521 aattggagga gagggaccaa agagaatcat tttggaagaa gttcgggaaa gataccagct 5581 gcatgacagg gtgcgtcttt tgggagcttt agaacacaag gatgttagaa atgtcttagt 5641 tcaaggacat atttttctga atacctccct tactgaagca ttctgcatgg cgatcgtgga 5701 agcagccagt tgtggtttac aggttgtaag taccagagtt ggtgggattc ctgaggtgct 5761 tccagaaaac cttattattt tatgtgagcc ttcagtaaaa tctttgtgtg aaggattgga 5821 aaaggctatt ttccaactga agtcagggac attgccagct ccagaaaaca tccataacat 5881 agtaaagact ttctacacct ggaggaatgt tgcagaaaga actgaaaagg tatatgaccg 5941 ggtatcagtg gaagctgtgt tgccaatgga caaacgactg gacagactta tttctcactg 6001 cggcccagta acaggctaca tctttgcttt gttggcagtt ttcaacttcc tcttcctcat 6061 tttcttgaga tggatgactc cagattctat cattgatgtt gcaatagatg ccactgggcc 6121 acggggtgcc tggactaata actattctca cagtaaaaga gggggtgaga ataatgagat 6181 atctgaaacc aggtaaggta cctgaggcca accttctatc agagttaaac ttttgacaag 6241 ggaacaaatc tcaaactgat ccatcagtca tgtagctagc tgtagagctt gcaacttaat 6301 agcagcagct gcccaatgcc atgtgaagta acaaactggt ttttggtttt tttttcccct 6361 tcagttttaa tgttatgtgt aatgtattta aacccttatt taaataaaac ttgttttcag 6421 aaatacctga cttgcagatt atttatatat agtcgatctt gtgcatataa aggaaccata 6481 gaaatgggca gtggaggatt cgtccacacc cacagcttct caacgggcct gaatttggcc 6541 aagaaaaacc tttatgagct tgtccctctc tggtcaactg gttttttgtt cgtttgtttt 6601 ttgagacatt cattgcaata gtgtgttgga attttttgtg tctctcactc ggtgcgtcct 6661 gcaggcgcgt tcataacttc gtatagcata cattatacga agttattatg cgagacctta 6721 tacgcacggt ctcaatccgc ggccgcgatt atcaaaaagg atcttcacct agatcctttt 6781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 6841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 6901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 6961 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa 7021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 7261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 7441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 7501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 7561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 7621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 7681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 7741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 7801 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt 7861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 8581 ttctgtggat aaccgtgcgg ccgcccctcg agaccttata cgcacggtct caaacg //