LOCUS       Exported                8458 bp DNA     circular SYN 09-FEB-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..58
                     /label=oLM1240.LP-LM.F1
                     /note="color: black; sequence: 
                     GCGGCCGCataacttcgtatagcatacattatacgaagttatggctccggtgcccgtca
                     g; added: 2020-09-28"
     primer_bind     complement(1..35)
                     /label=oSP218
                     /note="color: black; sequence: 
                     agtggatcccccgggctgcaggaattataacttcgtataatgtatgctatacgaagtta
                     t; added: 2020-09-28"
     protein_bind    1..34
                     /label=loxM
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #00ff00; direction: RIGHT"
     primer_bind     1..31
                     /label=oSP55_EF1_Alpha_For_ins_413
                     /note="color: black; sequence: 
                     cggtatcgataagcttgatatcgACGCGTataacttcgtataggatactttatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(1..30)
                     /label=oRO_141
                     /note="color: black; sequence: 
                     cttcgtataatgtatgctatacgaagttat; added: 2020-09-28"
     primer_bind     1..23
                     /label=oSP282_InsLoxP
                     /note="color: black; sequence: 
                     ataacttcgtatagcatacattatacgaagttatTTataacttcgtataggatacttta
                     ; added: 2020-09-28"
     primer_bind     3..34
                     /label=oRO_140
                     /note="color: black; sequence: 
                     aacttcgtataggatactttatacgaagttat; added: 2020-09-28"
     primer_bind     complement(4..36)
                     /label=oSP60_Cre_Rev_ins_413
                     /note="color: black; sequence: 
                     tagtggatcccccgggctgcaggACGCGTataacttcgtataatgtatgctatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(4..34)
                     /label=oSP340
                     /note="color: black; sequence: 
                     cgaaggatctggaggccaccatggtgtcgataacttcgtatagcatacattatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(5..34)
                     /label=oJML0019
                     /note="color: black; sequence: 
                     TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA
                     G; added: 2020-09-28"
     primer_bind     complement(8..34)
                     /label=oJML0389
                     /note="color: black; sequence: 
                     ttcgatACGCGTcgATAACTTCGTATAATGTATGCTATACG; added: 
                     2020-09-28"
     primer_bind     complement(9..35)
                     /label=oJML0253  Lvec-HTRA1-UL150-41homol_R
                     /note="color: black; sequence: 
                     ATCCATTCTGCAGCTCATGCATCAAAGAGTAATTATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0223
                     /note="color: black; sequence: 
                     GTTGTGCTGACAGTCATAGCTAGATAGTCCAATCATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0239  Lvec-HTRA1-LIMS3homology_R
                     /note="color: black; sequence: 
                     CTAATTTTCTACGATGGAGGCAATAAACAGACTCATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0250  Lvec-HTRA1-UL300-1homol_R
                     /note="color: black; sequence: 
                     CCAAGGCTGCCAAGTGGCAGAGCCCAGATGTGAAATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0251  Lvec-HTRA1-UL250-14homol_R
                     /note="color: black; sequence: 
                     TGGATTCCATCACCAGGGAGCTCAAGGTTCCAGAATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0252  Lvec-HTRA1-UL200-28homol_R
                     /note="color: black; sequence: 
                     ACCATGCCCGGCTGAGTTTTATATCTTTTGTAGAATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0254  Lvec-HTRA1-UL100-54homol_R
                     /note="color: black; sequence: 
                     GTACTCTTGATATCCTGTTGATGTGGTGGTCACAATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(9..34)
                     /label=oJML0255  Lvec-HTRA1-UL50-68homol_R
                     /note="color: black; sequence: 
                     GAATGGGAAGGAGAATCAATTTTCACTACTTACCATAACTTCGTATAAAGTATCCTATA
                     C; added: 2020-09-28"
     primer_bind     complement(12..58)
                     /label=oSP89_correcting_CEGS_Frag1_Rev
                     /note="color: black; sequence: 
                     CTGACGGGCACCGGAGCCataacttcgtataaagtatccta; added: 
                     2020-09-28"
     primer_bind     12..58
                     /label=oSP90_correcting_CEGS_Frag2_For
                     /note="color: black; sequence: 
                     taggatactttatacgaagttatGGCTCCGGTGCCCGTCAG; added: 
                     2020-09-28"
     primer_bind     39..60
                     /label=RB_065_GG
                     /note="color: black; sequence: 
                     ATGCGGTCTCGCAGTGGCTCCGGTGCCCGTCAGTG; added: 2020-09-28"
     primer_bind     40..59
                     /label=oLM878.dl5.F
                     /note="color: black; sequence: 
                     ATGGGGATTCTCGGGACCCCTCCTCTTCCTGGCTCCGGTGCCCGTCAGT; added: 
                     2020-09-28"
     promoter        41..1219
                     /standard_name="EF-1-alpha promoter"
                     /label=EF-1-alpha promoter
                     /note="Human EEF1A1 promoter
                     Recoded to eliminate AgeI site
                     "
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     41..70
                     /label=oSP257_DeletionofloxM
                     /note="color: black; sequence: 
                     aggtcgacggtatcgataagcttgatatcgGGCTCCGGTGCCCGTCAGTGGGCAGAGCG
                     C"
     primer_bind     41..60
                     /label=oJML0145
                     /note="color: black; sequence: 
                     GTGGTGCGTCTCTTCGGGGCTCCGGTGCCCGTCAGTG; added: 2020-09-28"
     primer_bind     41..60
                     /label=oLM1273.marker.R
                     /note="color: black; sequence: GGCTCCGGTGCCCGTCAGTG; added:
                     2020-09-28"
     primer_bind     41..58
                     /label=oLM1257.PL1to1050.F
                     /note="color: black; sequence: 
                     cttatccacaacattttgcgcacggttatgtggacgggccGGCTCCGGTGCCCGTCAG;
                      added: 2020-09-28"
     primer_bind     41..58
                     /label=oLM1204.PL2.F4
                     /note="color: black; sequence: 
                     CCCACCTTCTGGTCGATTGGGCTGGCGGGCTCCGGTGCCCGTCAG; added: 
                     2020-09-28"
     primer_bind     41..58
                     /label=oJML0049
                     /note="color: black; sequence: 
                     CATGAGTACCCAGAGTGGCTTCGGCTCCGGTGCCCGTCAG; added: 
                     2020-09-28"
     primer_bind     complement(70..87)
                     /label=oJML0189
                     /note="color: black; sequence: GGGACTGTGGGCGATGTG; added: 
                     2020-09-28"
     primer_bind     complement(76..92)
                     /label=oSP287_50bp2loxStuffer_Ef1alpha_R
                     /note="color: black; sequence: 
                     tcgagataacttcgtataaagtatcctatacgaagttatctcggggactgtgggc; 
                     added: 2020-09-28"
     primer_bind     complement(79..98)
                     /label=oRO_057
                     /note="color: black; sequence: ccaacttctcggggactgtg; added:
                     2020-09-28"
     primer_bind     complement(145..168)
                     /label=oSP66_CEGS_MMEJ_Jnctn1R
                     /note="color: black; sequence: ACGACATCACTTTCCCAGTTTACC; 
                     added: 2020-09-28"
     primer_bind     complement(207..230)
                     /label=oSP163_EF1Alpha_Rev_alt
                     /note="color: black; sequence: CGGCGACTACTGCACTTATATACG; 
                     added: 2020-09-28"
     primer_bind     complement(219..240)
                     /label=oLM1281.RgBlock.F
                     /note="color: black; sequence: AGAACGTTCACGGCGACTACTG; 
                     added: 2020-09-28"
     primer_bind     complement(270..290)
                     /label=oSP351_EF1alpha_250bp_NheI_R
                     /note="color: black; sequence: GCTAGCaaccacacacggcacttacct;
                     added: 2020-09-28"
     intron          272..1210
                     /standard_name="EF-1-alpha intron A"
                     /label=EF-1-alpha intron A
                     /note="color: #------"
     primer_bind     complement(274..292)
                     /label=oSP290_250bp2loxStuffer_EF1alpha_R
                     /note="color: black; sequence: 
                     ctcgagataacttcgtataaagtatcctatacgaagttatCggaaccacacacggcact
                     t; added: 2020-09-28"
     primer_bind     complement(401..460)
                     /label=oSP91_correcting_CEGS_Frag2_Rev
                     /note="color: black; sequence: 
                     CCAGGCCTCAACTCAAGCACGAGGCGAAGGGGCTCCTTAAGCGCAAGGCCTCGAACTCT
                     C; added: 2020-09-28"
     primer_bind     complement(515..540)
                     /label=oSP291_500bp2loxStuffer_EF1alpha_R
                     /note="color: black; sequence: 
                     taacttcgtataaagtatcctatacgaagttatCatggctagagacttatcgaaagcag
                     c; added: 2020-09-28"
     primer_bind     complement(515..540)
                     /label=oSP352_Ef1alpha_500bp_NheI_R
                     /note="color: black; sequence: 
                     GCTAGCatggctagagacttatcgaaagcagc; added: 2020-09-28"
     primer_bind     complement(588..607)
                     /label=DT1084R
                     /note="color: black; sequence: TTGGCCCGCATTTACAAGAC; added:
                     2020-09-28"
     primer_bind     662..672
                     /label=oRB_254_phPGK1-F1
                     /note="color: black; sequence: agagcagccgcgtccctgcg"
     primer_bind     715..734
                     /label=DT1043F
                     /note="color: black; sequence: AATCGGACGGGGGTAGTCTC; added:
                     2020-09-28"
     primer_bind     816..834
                     /label=oLM787.FAP.3.2-4.F
                     /note="color: black; sequence: CACCAGTTGCGTGAGCGGA; added: 
                     2020-09-28"
     primer_bind     820..839
                     /label=oRB_122_pEF1a-qPCR-F
                     /note="color: black; sequence: AGTTGCGTGAGCGGAAAGAT; added:
                     2020-09-28"
     primer_bind     916..937
                     /label=oLM770.FAP4.F
                     /note="color: black; sequence: CACACAAAGGAAAAGGGCCTTT; 
                     added: 2020-09-28"
     primer_bind     complement(949..968)
                     /label=oRB_123_pEF1a-qPCR-R
                     /note="color: black; sequence: TGGAGTCACATGAAGCGACG; added:
                     2020-09-28"
     primer_bind     complement(978..995)
                     /label=oLM773.FAP3.2.R
                     /note="color: black; sequence: ggtgcctggacggcgccc; added: 
                     2020-09-28"
     primer_bind     984..1003
                     /label=oRB_270_pEF1a-F
                     /note="color: black; sequence: CGTCCAGGCACCTCGATTAG; added:
                     2020-09-28"
     primer_bind     complement(1019..1042)
                     /label=oSP292_1000bp2loxStuffer_EF1alpha_R
                     /note="color: black; sequence: 
                     agataacttcgtataaagtatcctatacgaagttatCccccaacctaaagacgacgtac
                     t; added: 2020-09-28"
     primer_bind     complement(1019..1042)
                     /label=oSP353_Ef1alpha_1000bp_NheI_R
                     /note="color: black; sequence: 
                     GCTAGCccccaacctaaagacgacgtact; added: 2020-09-28"
     primer_bind     complement(1043..1065)
                     /label=oLM788.FAP.3.2-4.R
                     /note="color: black; sequence: AACTCCATCGCATAAAACCCCTC; 
                     added: 2020-09-28"
     primer_bind     1074..1097
                     /label=oSP67_CEGS_MMEJ_Jnctn2F
                     /note="color: black; sequence: ctgagtgggtggagactgaagtta; 
                     added: 2020-09-28"
     primer_bind     1092..1111
                     /label=oRO_128
                     /note="color: black; sequence: AAGTTAGGCCAGCTTGGCAC; added:
                     2020-09-28"
     primer_bind     1172..1191
                     /label=pEF1a-SeqF2
                     /note="color: black; sequence: CCTCAGACAGTGGTTCAAAG; added:
                     2020-09-28"
     primer_bind     complement(1195..1219)
                     /label=oJML0257 EF1aProm_R
                     /note="color: black; sequence: TCACGACACCTGAAATGGAAGAAAA; 
                     added: 2020-09-28"
     primer_bind     1197..1219
                     /label=oJML0246  mScarlet-EF1alphahomol_F
                     /note="color: black; sequence: 
                     TTCTTCCATTTCAGGTGTCGTGAATGGTGAGCAAGGGCGAGGC; added: 
                     2020-09-28"
     primer_bind     1197..1219
                     /label=oJML0240 mMaroon1-EF1ahomol_F
                     /note="color: black; sequence: 
                     TTCTTCCATTTCAGGTGTCGTGAATGGTGAGCAAGGGCGAG; added: 
                     2020-09-28"
     primer_bind     complement(1199..1226)
                     /label=oJML0146
                     /note="color: black; sequence: 
                     CACCACCGTCTCGGCTCTCACGACACCTGAAATGGAAG; added: 2020-09-28"
     primer_bind     1199..1219
                     /label=oSP217
                     /note="color: black; sequence: 
                     CTTCCATTTCAGGTGTCGTGATCTCTAGAAGCTGGGTACCAatggtgagcaagggcgag
                     ; added: 2020-09-28"
     primer_bind     complement(1199..1219)
                     /label=oJML0243  EF1aProm-mMaroonhomol_R
                     /note="color: black; sequence: 
                     CTCCTCGCCCTTGCTCACCATTCACGACACCTGAAATGGAAG; added: 
                     2020-09-28"
     primer_bind     1225..1246
                     /label=oJML0258 Puro-NcoI_F
                     /note="color: black; sequence: 
                     GTGTGCCATGGATGACCGAGTACAAGCCCACG; added: 2020-09-28"
     CDS             1226..1822
                     /codon_start=1
                     /standard_name="PuroR"
                     /product="Streptomyces alboniger Puromycin 
                     N-acetyltransferase"
                     /label=PuroR
                     /note="color: #0000ff"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1226..1246
                     /label=oJML0259 Puro-BamHI_F
                     /note="color: black; sequence: 
                     GTGTGGGATCCATGACCGAGTACAAGCCCACG; added: 2020-09-28"
     primer_bind     1226..1246
                     /label=oJML0260 Puro-SalI_F
                     /note="color: black; sequence: 
                     GTGTGGTCGACATGACCGAGTACAAGCCCACG; added: 2020-09-28"
     primer_bind     1226..1246
                     /label=oRO_124
                     /note="color: black; sequence: 
                     GGTGGAATTCatgaccgagtacaagcccacg; added: 2020-09-28"
     primer_bind     1226..1246
                     /label=oLM854.puro.F
                     /note="color: black; sequence: ATGACCGAGTACAAGCCCACG; 
                     added: 2020-09-28"
     primer_bind     1226..1245
                     /label=oJML0126
                     /note="color: black; sequence: 
                     GTGGTGGAAGACTCGAGCATGACCGAGTACAAGCCCAC; added: 2020-09-28"
     primer_bind     1228..1242
                     /label=oJML0394
                     /note="color: black; sequence: 
                     ATGATAACTTCGTATAGGATACTTTATACGAAGTTATCTCCATGGACCGAGTACAAGCC
                     ; added: 2020-09-28"
     primer_bind     complement(1367..1390)
                     /label=oRO_055
                     /note="color: black; sequence: cgtgaggaagagttcttgcagctc; 
                     added: 2020-09-28"
     primer_bind     complement(1367..1390)
                     /label=oSP561_Puro_R
                     /note="color: black; sequence: cgtgaggaagagttcttgcagctc; 
                     added: 2020-09-28"
     primer_bind     1401..1419
                     /label=oLM487.puro.seq.F2
                     /note="color: black; sequence: TCGACATCGGCAAGGTGTG; added: 
                     2020-09-28"
     primer_bind     complement(1510..1536)
                     /label=DT1101R
                     /note="color: black; sequence: gccagccggGaaccgctcaactcggcc;
                     added: 2020-09-28"
     primer_bind     1516..1542
                     /label=DT1100F
                     /note="color: black; sequence: gttgagcggttCccggctggccgcgca;
                     added: 2020-09-28"
     primer_bind     complement(1537..1556)
                     /label=oLM854.puro.R
                     /note="color: black; sequence: CTTCCATCTGTTGCTGCGCG; added:
                     2020-09-28"
     primer_bind     complement(1711..1721)
                     /label=oJML0083
                     /note="color: black; sequence: 
                     TTTTGTGATGCTCGTCAGGGGGGCGGAG; added: 2020-09-28"
     primer_bind     complement(1711..1721)
                     /label=oRO_062
                     /note="color: black; sequence: 
                     TTTTGTGATGCTCGTCAGGGGGGCGGAG; added: 2020-09-28"
     primer_bind     1801..1822
                     /label=oJML0137
                     /note="color: black; sequence: 
                     CATGACCCGCAAGCCCGGTGCCATGCCCACGCTACTGCGG; added: 
                     2020-09-28"
     primer_bind     complement(1805..1866)
                     /label=oJML0194
                     /note="color: black; sequence: 
                     CTGCCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCGGCACCGGGCTTGCGGGT;
                      added: 2020-09-28"
     primer_bind     complement(1805..1823)
                     /label=oJML0127
                     /note="color: black; sequence: 
                     CAGCCTGGTCGAACGCAGACGCGGCACCGGGCTTGCGGGT; added: 
                     2020-09-28"
     primer_bind     complement(1805..1822)
                     /label=oLM1245.LP-LM.R3
                     /note="color: black; sequence: 
                     gaaggttggcctcaggtaccTCAggcaccgggcttgcgggt; added: 
                     2020-09-28"
     primer_bind     1805..1822
                     /label=oLM1246.LP-LM.F4
                     /note="color: black; sequence: 
                     acccgcaagcccggtgccTGAggtacctgaggccaaccttc; added: 
                     2020-09-28"
     primer_bind     complement(1827..1846)
                     /label=oJML0129
                     /note="color: black; sequence: 
                     GTTGGTGGCGCCGCTGCCGTTAGCCTCCCCCATCTCCC"
     CDS             1829..1894
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1"
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1"
                     /note="color: #993366"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1829..1888)
                     /label=oJML0138
                     /note="color: black; sequence: 
                     GGGGTTCTCCTCCACGTCGCCGGCCTGCTTCAGCAGGCTGAAGTTGGTGGCGCCGCTGC
                     C; added: 2020-09-28"
     primer_bind     1835..1894
                     /label=oJML0139
                     /note="color: black; sequence: 
                     GGCGCCACCAACTTCAGCCTGCTGAAGCAGGCCGGCGACGTGGAGGAGAACCCCGGCCC
                     C"
     primer_bind     1835..1852
                     /label=RB_046_Gibs
                     /note="color: black; sequence: ggcgccaccaacttcagc; added: 
                     2020-09-28"
     primer_bind     complement(1879..1894)
                     /label=oSP556_Puro_iCasp9_R
                     /note="color: black; sequence: gggcccggggttctcc; added: 
                     2020-09-28"
     CDS             1901..3349
                     /codon_start=1
                     /product="Human mini PIGA
                     Recoded to eliminate PvuI, HindIII, NsiI sites
                     
                     "
                     /label=hmPIGA
                     /note="color: #00ffff"
                     /translation="ACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNM
                     GGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATT
                     LFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSS
                     VLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRH
                     DSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHD
                     RVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLP
                     ENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDR
                     VSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSIIDVAIDATG
                     PRGAWTNNYSHSKRGGENNEISETR"
     primer_bind     1901..1917
                     /label=oJML0012
                     /note="color: black; sequence: 
                     CTAGGCTTTTGCAAAAAGCTATGGCCTGTAGAGGAGGAGC; added: 
                     2020-09-28"
     primer_bind     2073..2105
                     /label=oRB_234_oSP360
                     /note="color: black; sequence: 
                     GCCTGATTGAAAGAGGGCATAAGGTTATAATTG; added: 2020-09-28"
     primer_bind     complement(2174..2199)
                     /label=oRB_235_oSP48
                     /note="color: black; sequence: gactggttgtacatgactttcagagg; 
                     added: 2020-09-28"
     primer_bind     2184..2216
                     /label=oSP125_2-2_PCR1_For
                     /note="color: black; sequence: 
                     TCATGTACAACCAGTCTACAGCCACGACCCTCTCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     2184..2208
                     /label=oSP126_2-2_PCR2_For
                     /note="color: black; sequence: 
                     atacattatacgaagttatACGCGCCTGCATGAATTCATGTACAACCAGTCTACAGCCA
                     C; added: 2020-09-28"
     primer_bind     complement(2197..2216)
                     /label=oSP86_PIGA_gRNA_2-2_F
                     /note="color: black; sequence: 
                     TAATACGACTCACTATAGAGAGGGTCGTGGCTGTAGAC; added: 2020-09-28"
     primer_bind     2197..2216
                     /label=oSP87_PIGA_gRNA_2-2_R
                     /note="color: black; sequence: 
                     TTCTAGCTCTAAAACGTCTACAGCCACGACCCTCT; added: 2020-09-28"
     primer_bind     2200..2222
                     /label=oSP51_PIGA_gRNA_2-2_HA1_Rev_w/o_homology
                     /note="color: black; sequence: tacagccacgaccctctttcaca; 
                     added: 2020-09-28"
     primer_bind     complement(2320..2338)
                     /label=oSP107_gRNA_2-2_R
                     /note="color: black; sequence: AAGCCCCATTGTCTTGGCG; added: 
                     2020-09-28"
     primer_bind     2369..2390
                     /label=oSP162_2-5_For_alt
                     /note="color: black; sequence: GGATTTGCTGATGTCAGCTCGG; 
                     added: 2020-09-28"
     primer_bind     2372..2391
                     /label=oRB_150_hPIGA_F
                     /note="color: black; sequence: TTTGCTGATGTCAGCTCGGT; added:
                     2020-09-28"
     primer_bind     complement(2476..2500)
                     /label=oSP50_PIGA_gRNA_2-5_HA2_For_w/o_homology
                     /note="color: black; sequence: ttcaggattcagtgctgctcttagt; 
                     added: 2020-09-28"
     primer_bind     2485..2517
                     /label=oSP122_2-5_PCR1_For
                     /note="color: black; sequence: 
                     AGCACTGAATCCTGAAATAGTGTCCGTCATTCCCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     2485..2509
                     /label=oSP124_2-5_PCR2_For
                     /note="color: black; sequence: 
                     atacattatacgaagttatACGCGCCTGCATGAATAGCACTGAATCCTGAAATAGTGTC
                     C; added: 2020-09-28"
     primer_bind     complement(2498..2519)
                     /label=oSP84_PIGA_gRNA_2-5_F
                     /note="color: black; sequence: 
                     TAATACGACTCACTATAGGAATGACGGACACTATTTC; added: 2020-09-28"
     primer_bind     2498..2517
                     /label=oSP85_PIGA_gRNA_2-5_R
                     /note="color: black; sequence: 
                     TTCTAGCTCTAAAACGAAATAGTGTCCGTCATTCC; added: 2020-09-28"
     primer_bind     2501..2526
                     /label=oSP53_PIGA_gRNA_2-5_HA1_Rev_w/o_homology
                     /note="color: black; sequence: atagtgtccgtcattcctaatgctgt; 
                     added: 2020-09-28"
     primer_bind     complement(2546..2565)
                     /label=oSP361_JB17709_R
                     /note="color: black; sequence: TGCCTTCTAAATGGGTCTGG; added:
                     2020-09-28"
     primer_bind     complement(2740..2759)
                     /label=oRB_151_hPIGA_R
                     /note="color: black; sequence: CCAAAAGACGCACCCTGTCA; added:
                     2020-09-28"
     primer_bind     3253..3274
                     /label=oRO_058
                     /note="color: black; sequence: tgttgcaatagatgccactggg; 
                     added: 2020-09-28"
     primer_bind     complement(3317..3349)
                     /label=oRB_129_PIGA-R-KpnI
                     /note="color: black; sequence: 
                     acgtggtaccttacctggtttcagatatctcattattctcaccccc; added: 
                     2020-09-28"
     primer_bind     complement(3320..3349)
                     /label=oJML0006
                     /note="color: black; sequence: 
                     GCAACTAGAAGGCACAGTCGCTACCTGGTTTCAGATATCTCATTATTCTCACC; 
                     added: 2020-09-28"
     primer_bind     complement(3325..3349)
                     /label=oJML0261 PIGA-XbaI_R
                     /note="color: black; sequence: 
                     GTGTGTCTAGACCTGGTTTCAGATATCTCATTATTC; added: 2020-09-28"
     CDS             3356..3421
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1, recoded by
                     Jon Laurent"
                     /label=P2A1
                     /note="color: #993366"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer_bind     3356..3377
                     /label=oRB_128_P2A-F-XbaI
                     /note="color: black; sequence: 
                     catggacgagctgtacaagtctagaggctcaggcgccaccaacttta; added: 
                     2020-09-28"
     primer_bind     3362..3379
                     /label=RB_046_Gibs
                     /note="color: black; sequence: ggcgccaccaacttcagc; added: 
                     2020-09-28"
     primer_bind     complement(3379..3395)
                     /label=oJML0194
                     /note="color: black; sequence: 
                     CTGCCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCGGCACCGGGCTTGCGGGT;
                      added: 2020-09-28"
     primer_bind     3385..3418
                     /label=oSP555_Puro_iCasp9_F
                     /note="color: black; sequence: 
                     gaagcaggcaggcgacgtcgaggagaaccccggc; added: 2020-09-28"
     primer_bind     complement(3406..3417)
                     /label=oSP556_Puro_iCasp9_R
                     /note="color: black; sequence: gggcccggggttctcc; added: 
                     2020-09-28"
     primer_bind     3423..3447
                     /label=oJML0246  mScarlet-EF1alphahomol_F
                     /note="color: black; sequence: 
                     TTCTTCCATTTCAGGTGTCGTGAATGGTGAGCAAGGGCGAGGC; added: 
                     2020-09-28"
     primer_bind     3423..3445
                     /label=oJML0240 mMaroon1-EF1ahomol_F
                     /note="color: black; sequence: 
                     TTCTTCCATTTCAGGTGTCGTGAATGGTGAGCAAGGGCGAG; added: 
                     2020-09-28"
     primer_bind     3427..3446
                     /label=oSP198_mCherry_Pab1_F
                     /note="color: black; sequence: 
                     GTCTTTCAAAAAGGAGCAAGAACAACAAACTGAGCAAGCTATGGTGAGCAAGGGCGAGG
                     ; added: 2020-09-28"
     CDS             3428..4123
                     /codon_start=1
                     /product="https://www.fpbase.org/protein/mscarlet/
                     Recoded to eliminate ScaI, EagI sites"
                     /label=mScarlet
                     /note="color: #da0025"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3428..3446)
                     /label=GFP-SeqR1
                     /note="color: black; sequence: TCCTCGCCCTTGCTCACCAT; added:
                     2020-09-28"
     primer_bind     3428..3446
                     /label=oSP210_mCherry_Ded1_F
                     /note="color: black; sequence: 
                     CTGGGGTAACAGCGGTGGTTCAAACAACTCTTCTTGGTGGATGGTGAGCAAGGGCGAGG
                     ; added: 2020-09-28"
     primer_bind     3428..3446
                     /label=oLM952.750.gfp.3.F
                     /note="color: black; sequence: 
                     cctgcatccatctagatctcgatggtgagcaagggcgagg; added: 
                     2020-09-28"
     primer_bind     3428..3445
                     /label=oSP217
                     /note="color: black; sequence: 
                     CTTCCATTTCAGGTGTCGTGATCTCTAGAAGCTGGGTACCAatggtgagcaagggcgag
                     ; added: 2020-09-28"
     primer_bind     3428..3445
                     /label=oSP294_2000bp2loxStuffer_mCherry_F
                     /note="color: black; sequence: 
                     ccgatcggtacctaccaccatggtgagcaagggcgag; added: 2020-09-28"
     primer_bind     3428..3445
                     /label=oRO_122
                     /note="color: black; sequence: 
                     GGTGCGGCCGATGGTGAGCAAGGGCGAG; added: 2020-09-28"
     primer_bind     3772..3782
                     /label=oLM773.FAP3.2.R
                     /note="color: black; sequence: ggtgcctggacggcgccc; added: 
                     2020-09-28"
     primer_bind     complement(3832..3861)
                     /label=oJML0248 midmScarlet-noBbsI_R
                     /note="color: black; sequence: 
                     TCCCAGCCCATTGTTTTCTTCTGCATTACG; added: 2020-09-28"
     primer_bind     3833..3862
                     /label=oJML0249 midmScarlet-noBbsI_F
                     /note="color: black; sequence: 
                     GTAATGCAGAAGAAAACAATGGGCTGGGAA; added: 2020-09-28"
     primer_bind     complement(4103..4128)
                     /label=oSP224_2lox_LHM21_Rev
                     /note="color: black; sequence: 
                     ctgcagcatatcatggcgtgtaatatgagagacgccttacttgtacagctcgtccatgc
                     c; added: 2020-09-28"
     primer_bind     complement(4103..4127)
                     /label=oSP295_2000bp2loxStuffer_mCherry_R
                     /note="color: black; sequence: 
                     gataacttcgtataaagtatcctatacgaagttatCttacttgtacagctcgtccatgc
                     c; added: 2020-09-28"
     primer_bind     complement(4103..4127)
                     /label=oSP354_mCherry_NheI_R
                     /note="color: black; sequence: 
                     GCTAGCttacttgtacagctcgtccatgcc; added: 2020-09-28"
     primer_bind     complement(4103..4126)
                     /label=oSP544_GFP_Lenti_R
                     /note="color: black; sequence: 
                     acaaattttgtaatccagaggttgattaggatctatttacttgtacagctcgtccatgc
                     c; added: 2020-09-28"
     primer_bind     complement(4103..4126)
                     /label=oSP296_mCherry2loxStuffer_mCherry_bghpA_R
                     /note="color: black; sequence: 
                     CAGATGGCTGGCAACTAGAAGGCACAGTCGttacttgtacagctcgtccatgcc; 
                     added: 2020-09-28"
     primer_bind     complement(4103..4126)
                     /label=oSP199_mCherry_R
                     /note="color: black; sequence: TTACTTGTACAGCTCGTCCATGCC; 
                     added: 2020-09-28"
     primer_bind     complement(4103..4125)
                     /label=oRO_123
                     /note="color: black; sequence: 
                     GGTGGGTACCCCATGGCTACTTGTACAGCTCGTCCATGCC; added: 
                     2020-09-28"
     primer_bind     complement(4103..4123)
                     /label=oSP148_mCherry_Gly_Ser_R
                     /note="color: black; sequence: 
                     ACCACTAGCCTTACCAGCACTACCAGCACTCTTGTACAGCTCGTCCATGCC; added:
                     2020-09-28"
     primer_bind     complement(4104..4125)
                     /label=oJML0174
                     /note="color: black; sequence: 
                     GTGGTGGAAGACTCAGTACTACTTGTACAGCTCGTCCATGC; added: 
                     2020-09-28"
     primer_bind     complement(4104..4125)
                     /label=oJML0066
                     /note="color: black; sequence: 
                     GTGGTGGCGGCCGCCTACTTGTACAGCTCGTCCATGC; added: 2020-09-28"
     primer_bind     complement(4104..4123)
                     /label=RB_066_GG
                     /note="color: black; sequence: 
                     GCATGGTCTCTTGCCCTTGTACAGCTCGTCCATGC; added: 2020-09-28"
     primer_bind     complement(4104..4123)
                     /label=oJML0247 mScarlet-P2A8homol_R
                     /note="color: black; sequence: 
                     CCTGCTTCAGCAGGGAGAAGTTAGTCGCGCCTGACCCCTTGTACAGCTCGTCCATGC; 
                     added: 2020-09-28"
     primer_bind     4105..4124
                     /label=oRB_128_P2A-F-XbaI
                     /note="color: black; sequence: 
                     catggacgagctgtacaagtctagaggctcaggcgccaccaacttta; added: 
                     2020-09-28"
     primer_bind     complement(4108..4126)
                     /label=GFP-ClaI-R
                     /note="color: black; sequence: 
                     GGCCATCGATTTACTTGTACAGCTCGTCC; added: 2020-09-28"
     primer_bind     complement(4126..4146)
                     /label=oLM1245.LP-LM.R3
                     /note="color: black; sequence: 
                     gaaggttggcctcaggtaccTCAggcaccgggcttgcgggt; added: 
                     2020-09-28"
     primer_bind     4126..4146
                     /label=oLM1246.LP-LM.F4
                     /note="color: black; sequence: 
                     acccgcaagcccggtgccTGAggtacctgaggccaaccttc; added: 
                     2020-09-28"
     polyA_signal    4133..4538
                     /label=hEIF1pA
                     /note="Human EIF1 poly adenylation signal"
                     /note="color: #a6acb3"
     primer_bind     4133..4154
                     /label=oJML0135
                     /note="color: black; sequence: 
                     GGGAGGCAGAGGGTTTCCCTGCCACAGCTTGATGAAGATGAGGCCAACCTTCTATCAGA
                     G; added: 2020-09-28"
     primer_bind     4133..4154
                     /label=oJML0175
                     /note="color: black; sequence: 
                     GTGGTGGAAGACTCTACTTGAGGCCAACCTTCTATCAGAG; added: 
                     2020-09-28"
     primer_bind     complement(4177..4205)
                     /label=oSP563_EF1AlphapA_R
                     /note="color: black; sequence: 
                     ctacatgactgatggatcagtttgagatt; added: 2020-09-28"
     primer_bind     complement(4195..4218)
                     /label=oRO_059
                     /note="color: black; sequence: gctctacagctagctacatgactg; 
                     added: 2020-09-28"
     primer_bind     complement(4240..4260)
                     /label=oRO_129
                     /note="color: black; sequence: TACTTCACATGGCATTGGGCA; 
                     added: 2020-09-28"
     primer_bind     4438..4456
                     /label=oJML0190
                     /note="color: black; sequence: ACCCACAGCTTCTCAACGG; added: 
                     2020-09-28"
     primer_bind     4438..4456
                     /label=oRO_060
                     /note="color: black; sequence: ACCCACAGCTTCTCAACGG; added: 
                     2020-09-28"
     primer_bind     complement(4508..4538)
                     /label=oJML0136
                     /note="color: black; sequence: 
                     GTGGTGGAAGACTCTGAATGTCTCAAAAAACAAACGAACAAAAAACCAG; added: 
                     2020-09-28"
     primer_bind     4539..4573
                     /label=oSP282_InsLoxP
                     /note="color: black; sequence: 
                     ataacttcgtatagcatacattatacgaagttatTTataacttcgtataggatacttta
                     ; added: 2020-09-28"
     protein_bind    4539..4572
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(4539..4572)
                     /label=oSP218
                     /note="color: black; sequence: 
                     agtggatcccccgggctgcaggaattataacttcgtataatgtatgctatacgaagtta
                     t; added: 2020-09-28"
     primer_bind     4539..4569
                     /label=oSP55_EF1_Alpha_For_ins_413
                     /note="color: black; sequence: 
                     cggtatcgataagcttgatatcgACGCGTataacttcgtataggatactttatacgaag
                     t; added: 2020-09-28"
     primer_bind     4539..4569
                     /label=oSP340
                     /note="color: black; sequence: 
                     cgaaggatctggaggccaccatggtgtcgataacttcgtatagcatacattatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(4539..4568)
                     /label=oRO_141
                     /note="color: black; sequence: 
                     cttcgtataatgtatgctatacgaagttat; added: 2020-09-28"
     primer_bind     4539..4564
                     /label=oJML0224
                     /note="color: black; sequence: 
                     ACCTCCTAGGCTCAAGCGATCTCTTGCTTTAGCCATAACTTCGTATAGCATACATTATA
                     C; added: 2020-09-28"
     primer_bind     4539..4564
                     /label=oJML0228
                     /note="color: black; sequence: 
                     TTTTCTGCTTCTCACTGCTGAGCAGACACATGTGATAACTTCGTATAGCATACATTATA
                     C; added: 2020-09-28"
     primer_bind     4539..4564
                     /label=oJML0256  Rvec-HTRA1-UL80homol_F
                     /note="color: black; sequence: 
                     GGGAGAAAGCACCTTCCCAGGATGCGTTGTATTTATAACTTCGTATAGCATACATTATA
                     C; added: 2020-09-28"
     primer_bind     4539..4564
                     /label=oJML0395
                     /note="color: black; sequence: 
                     GTGTGCGGCCGCCATGATAACTTCGTATAGGATACTTTATAC; added: 
                     2020-09-28"
     primer_bind     4539..4557
                     /label=oLM731.FAP.3HPRT.F
                     /note="color: black; sequence: 
                     AATCGCCTTGCAGCACATCCCCCTTTCGCGAGCTCGAATTCATAACTTCGTATAGCATA
                     C; added: 2020-09-28"
     primer_bind     4539..4553
                     /label=loxP-NotI-F
                     /note="color: black; sequence: 
                     ATCGGCGGCCGCTGGAATTATAACTTCGTATAGC; added: 2020-09-28"
     primer_bind     4541..4572
                     /label=oRO_140
                     /note="color: black; sequence: 
                     aacttcgtataggatactttatacgaagttat; added: 2020-09-28"
     primer_bind     complement(4542..4572)
                     /label=oSP60_Cre_Rev_ins_413
                     /note="color: black; sequence: 
                     tagtggatcccccgggctgcaggACGCGTataacttcgtataatgtatgctatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(4543..4572)
                     /label=oJML0019
                     /note="color: black; sequence: 
                     TCCACACCCTAACTGACACACATTCCACAGATAACTTCGTATAAAGTATCCTATACGAA
                     G; added: 2020-09-28"
     primer_bind     4545..4572
                     /label=oSP83_CEGS_ori_Sbf1_For
                     /note="color: black; sequence: 
                     tcgtatagcatacattatacgaagttatACGCGCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     complement(4546..4572)
                     /label=oJML0389
                     /note="color: black; sequence: 
                     ttcgatACGCGTcgATAACTTCGTATAATGTATGCTATACG; added: 
                     2020-09-28"
     primer_bind     4550..4572
                     /label=oSP81_CEGS_Sbf1_For
                     /note="color: black; sequence: 
                     tagcatacattatacgaagttatACGCGCCTGCAGGcttggtctgacagttaccaatgc
                     t; added: 2020-09-28"
     primer_bind     4554..4572
                     /label=oSP126_2-2_PCR2_For
                     /note="color: black; sequence: 
                     atacattatacgaagttatACGCGCCTGCATGAATTCATGTACAACCAGTCTACAGCCA
                     C; added: 2020-09-28"
     primer_bind     4554..4572
                     /label=oSP124_2-5_PCR2_For
                     /note="color: black; sequence: 
                     atacattatacgaagttatACGCGCCTGCATGAATAGCACTGAATCCTGAAATAGTGTC
                     C; added: 2020-09-28"
     primer_bind     complement(4557..4573)
                     /label=oJML0188
                     /note="color: black; sequence: CTCAGGCAATAACTTCGTATAATG; 
                     added: 2020-09-28"
     primer_bind     complement(4653..4672)
                     /label=oSP215_PIF_Act1t_R
                     /note="color: black; sequence: 
                     gagaggtacatacataaacatacgcgcacaaaagcagagaggcagtgactccgtctctc
                     c; added: 2020-09-28"
     primer_bind     complement(4658..4676)
                     /label=oSP228_PIF6_20aa_Act1t_Rev
                     /note="color: black; sequence: 
                     gagaggtacatacataaacatacgcgcacaaaagcagagaggttggcagtgactccgtc
                     ; added: 2020-09-28"
     primer_bind     complement(4680..4699)
                     /label=oRO_063
                     /note="color: black; sequence: caggaaacagctatgaccgt; added:
                     2020-09-28"
     primer_bind     complement(4683..4699)
                     /label=GENEWIZ_M13R
                     /note="color: black; sequence: CAGGAAACAGCTATGAC; added: 
                     2020-09-28"
     primer_bind     complement(4717..4735)
                     /label=oLM736.541.F
                     /note="color: black; sequence: cgagcgcagcgagtcagtg; added: 
                     2020-09-28"
     primer_bind     complement(4743..4752)
                     /label=oJML0138
                     /note="color: black; sequence: 
                     GGGGTTCTCCTCCACGTCGCCGGCCTGCTTCAGCAGGCTGAAGTTGGTGGCGCCGCTGC
                     C; added: 2020-09-28"
     primer_bind     complement(4822..4841)
                     /label=oJML0268 LPv4-Seq_F
                     /note="color: black; sequence: GCTGGCCTTTTGCTCACATG; added:
                     2020-09-28"
     primer_bind     complement(4873..4892)
                     /label=oJML0015
                     /note="color: black; sequence: GCCTATGGAAAAACGCCAGC"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     4882..4906
                     /label=oJML0053
                     /note="color: black; sequence: TTTCCATAGGCTCCGCCCCCCTGAC; 
                     added: 2020-09-28"
     primer_bind     4882..4906
                     /label=oRO_067
                     /note="color: black; sequence: TTTCCATAGGCTCCGCCCCCCTGAC; 
                     added: 2020-09-28"
     primer_bind     4882..4900
                     /label=oSP83_CEGS_ori_Sbf1_For
                     /note="color: black; sequence: 
                     tcgtatagcatacattatacgaagttatACGCGCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     4882..4900
                     /label=oSP122_2-5_PCR1_For
                     /note="color: black; sequence: 
                     AGCACTGAATCCTGAAATAGTGTCCGTCATTCCCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     4882..4900
                     /label=oSP125_2-2_PCR1_For
                     /note="color: black; sequence: 
                     TCATGTACAACCAGTCTACAGCCACGACCCTCTCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     complement(4892..4919)
                     /label=oJML0083
                     /note="color: black; sequence: 
                     TTTTGTGATGCTCGTCAGGGGGGCGGAG; added: 2020-09-28"
     primer_bind     complement(4892..4919)
                     /label=oRO_062
                     /note="color: black; sequence: 
                     TTTTGTGATGCTCGTCAGGGGGGCGGAG; added: 2020-09-28"
     primer_bind     4912..4971
                     /label=oJML0018
                     /note="color: black; sequence: 
                     TCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
                     A; added: 2020-09-28"
     primer_bind     complement(4912..4971)
                     /label=oJML0027
                     /note="color: black; sequence: 
                     TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTG
                     A; added: 2020-09-28"
     primer_bind     complement(4941..4961)
                     /label=oLM437.H4.R
                     /note="color: black; sequence: ATAGTCCTGTCGGGTTTCGCC; 
                     added: 2020-09-28"
     primer_bind     complement(4956..4979)
                     /label=oJML0234_
                     /note="color: black; sequence: GAAACGCCTGGTATCTTTATAGTC; 
                     added: 2020-09-28"
     primer_bind     complement(5000..5018)
                     /label=oSP164_Bacparts_Rev_alt
                     /note="color: black; sequence: gggtcggaacaggagagcg; added: 
                     2020-09-28"
     primer_bind     5163..5182
                     /label=oJML0045
                     /note="color: black; sequence: GCTGCGCCTTATCCGGTAAC; added:
                     2020-09-28"
     primer_bind     complement(5163..5182)
                     /label=oJML0046
                     /note="color: black; sequence: GTTACCGGATAAGGCGCAGC; added:
                     2020-09-28"
     primer_bind     complement(5163..5182)
                     /label=oRO_068
                     /note="color: black; sequence: GTTACCGGATAAGGCGCAGC; added:
                     2020-09-28"
     primer_bind     5261..5280
                     /label=oRO_291_Ori_new.Rv
                     /note="color: black; sequence: aggtatgtaggcggtgctac; added:
                     2021-04-16"
     primer_bind     complement(5382..5401)
                     /label=oRO_290_Ori_new.Fw
                     /note="color: black; sequence: ttgtttgccggatcaagagc; added:
                     2021-04-16"
     terminator      5547..5640
                     /standard_name="AmpR Terminator"
                     /label=Ter
                     /note="color: #c0c0c0"
     primer_bind     complement(5553..5586)
                     /label=oJML0269 LPv4-Seq_R
                     /note="color: black; sequence: 
                     TAATTTAAAAGGATCTAGGTGAAGATCCTTTTTG; added: 2020-09-28"
     primer_bind     complement(5623..5650)
                     /label=oJML0270 LPv4-Seq_Rb
                     /note="color: black; sequence: 
                     GCATTGGTAACTGTCAGACCAAGTTTAC; added: 2020-09-28"
     primer_bind     complement(5623..5650)
                     /label=oRO_061
                     /note="color: black; sequence: 
                     GCATTGGTAACTGTCAGACCAAGTTTAC; added: 2020-09-28"
     primer_bind     5628..5651
                     /label=oSP81_CEGS_Sbf1_For
                     /note="color: black; sequence: 
                     tagcatacattatacgaagttatACGCGCCTGCAGGcttggtctgacagttaccaatgc
                     t; added: 2020-09-28"
     CDS             complement(5641..6501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5641 .. 6432 / #ccffcc
                        2: 6433 .. 6501 / #ccffcc / signal sequence
                     Cleavage site after base 6432"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     5765..5805
                     /label=LM129.QC.Amp.F
                     /note="color: black; sequence: 
                     gctgcaatgataccgcgagatccacgctcaccggctccaga; added: 
                     2020-09-28"
     primer_bind     5768..5807
                     /label=oJML0067
                     /note="color: black; sequence: 
                     GCAATGATACCGCGAGATCCACGCTCACCGGCTCCAGATT; added: 
                     2020-09-28"
     primer_bind     5835..5845
                     /label=oSP257_DeletionofloxM
                     /note="color: black; sequence: 
                     aggtcgacggtatcgataagcttgatatcgGGCTCCGGTGCCCGTCAGTGGGCAGAGCG
                     C"
     primer_bind     6415..6474
                     /label=oJML0029
                     /note="color: black; sequence: 
                     CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAA
                     G; added: 2020-09-28"
     primer_bind     complement(6415..6474)
                     /label=oJML0030
                     /note="color: black; sequence: 
                     CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGT
                     G; added: 2020-09-28"
     misc_feature    complement(6502..6632)
                     /standard_name="AmpR Promoter"
                     /label=pAmpR
                     /note="AmpR (bla) promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     6538..6559
                     /label=oLM486.puro.seq.F1
                     /note="color: black; sequence: gggttattgtctcatgagcgga; 
                     added: 2020-09-28"
     primer_bind     6590..6609
                     /label=DT425F pRS-origin
                     /note="color: black; sequence: CAAATAGGGGTTCCGCGCAC; added:
                     2020-09-28"
     primer_bind     complement(6613..6632)
                     /label=oJML0044
                     /note="color: black; sequence: 
                     CACACCGCATAGATCCGTCGAGTTCAAGAGGAAAAGGACCCAGGTGGCACTTTTCGGGG
                     A; added: 2020-09-28"
     primer_bind     6614..6632
                     /label=oLM447.BACconvert.F
                     /note="color: black; sequence: 
                     catatcaccagctcaccgtctttcattgccatacggaattcCCCCGAAAAGTGCCACCT
                     G; added: 2020-09-28"
     primer_bind     6614..6632
                     /label=oLM862.SMCF.rep.R
                     /note="color: black; sequence: 
                     CTTCCGTTGCCCCAGTAGCATCTCTGTCTGGTGACCTTGAccccgaaaagtgccacctg
                     ; added: 2020-09-28"
     primer_bind     complement(6617..6636)
                     /label=oLM925.leftvec1.R1
                     /note="color: black; sequence: 
                     AGCACGTGATGAAAAGGACCacgtcaggtggcacttttcg; added: 
                     2020-09-28"
     primer_bind     complement(6677..6697)
                     /label=LM126.overwriteBsmBI.R
                     /note="color: black; sequence: 
                     gcttacagacaagctgtgacaaagggcctcgtgatacgcct; added: 
                     2020-09-28"
     primer_bind     6679..6698
                     /label=oRO_056
                     /note="color: black; sequence: gcgtatcacgaggccctttc; added:
                     2020-09-28"
     primer_bind     6698..6714
                     /label=oSP326_Cox2R
                     /note="color: black; sequence: 
                     gggtccttttcatcacgtgctataaaaataattataatttcgacgttgtaaaacgacgg
                     c; added: 2020-09-28"
     primer_bind     6698..6714
                     /label=oLM422.puc57.R
                     /note="color: black; sequence: ACGACGTTGTAAAACGACGGC; 
                     added: 2020-09-28"
     primer_bind     6702..6717
                     /label=GENEWIZ_M13F
                     /note="color: black; sequence: GTAAAACGACGGCCAG; added: 
                     2020-09-28"
     primer_bind     6785..6803
                     /label=oRO_130
                     /note="color: black; sequence: GGGCATCCCTGCAggtaag; added: 
                     2020-09-28"
     primer_bind     6790..6817
                     /label=oJML0397
                     /note="color: black; sequence: 
                     GAAACTAAATATTGGTAGCTACCTTAGGCCCGTCCCTGCAGGTAAGATACATTGATGAG
                     T; added: 2020-09-28"
     primer_bind     6798..6823
                     /label=oLM1181.PL2.R2
                     /note="color: black; sequence: 
                     GCATTTGCAACCTAAGGGGtaagatacattgatgagtttggac"
     polyA_signal    6800..6921
                     /label=SV40pA
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     6800..6817
                     /label=oLM953.750.gfp.3.R
                     /note="color: black; sequence: 
                     ccagacatgataagatacattgatgagt; added: 2020-09-28"
     primer_bind     complement(6810..6831)
                     /label=LM202.HPRT.gblock.R
                     /note="color: black; sequence: TGTGGTTTGTCCAAACTCATCA"
     primer_bind     complement(6820..6845)
                     /label=LM198.HPRT.I2.R3
                     /note="color: black; sequence: CACTGCATTCTAGTTGTGGTTTGTCC"
     primer_bind     complement(6820..6845)
                     /label=oSP256_SV40pA_F
                     /note="color: black; sequence: cactgcattctagttgtggtttgtcc"
     primer_bind     complement(6822..6846)
                     /label=oLM860.FAP.polyA.seq.R
                     /note="color: black; sequence: TCACTGCATTCTAGTTGTGGTTTGT"
     primer_bind     6857..6888
                     /label=LM255.NeoR
                     /note="color: black; sequence: 
                     TTATTTGTGAAATTTGTGATGCTATTGCTTTA"
     primer_bind     6865..6884
                     /label=GENEWIZ_SV40pA-R
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     6865..6884
                     /label=LM244.SV40pA-R
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     complement(6881..6921)
                     /label=oRO_127
                     /note="color: black; sequence: 
                     GGTGGGATCCaacttgtttattgcagcttataatggttacaaataaagcaa; added:
                     2020-09-28"
     primer_bind     6922..6944
                     /label=oRO_125
                     /note="color: black; sequence: 
                     GGTGGGATCCTTAgttagcctcccccatctccc; added: 2020-09-28"
     CDS             complement(6925..7917)
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995).
                     Recoded to remove several restriction sites."
                     /label=Delta-TKmod
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /note="color: #993366"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     6925..6944
                     /label=oJML0129
                     /note="color: black; sequence: 
                     GTTGGTGGCGCCGCTGCCGTTAGCCTCCCCCATCTCCC"
     primer_bind     complement(7389..7398)
                     /label=oJML0015
                     /note="color: black; sequence: GCCTATGGAAAAACGCCAGC"
     primer_bind     complement(7589..7598)
                     /label=oJML0139
                     /note="color: black; sequence: 
                     GGCGCCACCAACTTCAGCCTGCTGAAGCAGGCCGGCGACGTGGAGGAGAACCCCGGCCC
                     C"
     primer_bind     complement(7900..7917)
                     /label=oJML0137
                     /note="color: black; sequence: 
                     CATGACCCGCAAGCCCGGTGCCATGCCCACGCTACTGCGG; added: 
                     2020-09-28"
     primer_bind     7918..7934
                     /label=oRO_126
                     /note="color: black; sequence: GGTGGAATTCcctccaacgagggagcc;
                     added: 2020-09-28"
     promoter        complement(7923..8406)
                     /label=phPGK1_opt
                     /note="human phosphoglycerate kinase 1 promoter
                     Recoded for restriction sites: EagI, BsaI sites mutated 
                     with single bp based on alignment with mouse PGK promoter. 
                     BsmbI, SpeI sites mutated semi-randomly (just guessed and 
                     hoped)
                     "
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8064..8082)
                     /label=oRO_131
                     /note="color: black; sequence: caatagcggctgctcagca; added: 
                     2020-09-28"
     primer_bind     complement(8103..8112)
                     /label=oSP257_DeletionofloxM
                     /note="color: black; sequence: 
                     aggtcgacggtatcgataagcttgatatcgGGCTCCGGTGCCCGTCAGTGGGCAGAGCG
                     C"
     primer_bind     complement(8216..8225)
                     /label=oSP83_CEGS_ori_Sbf1_For
                     /note="color: black; sequence: 
                     tcgtatagcatacattatacgaagttatACGCGCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     complement(8216..8225)
                     /label=oSP122_2-5_PCR1_For
                     /note="color: black; sequence: 
                     AGCACTGAATCCTGAAATAGTGTCCGTCATTCCCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     complement(8216..8225)
                     /label=oSP125_2-2_PCR1_For
                     /note="color: black; sequence: 
                     TCATGTACAACCAGTCTACAGCCACGACCCTCTCCTGCAGGtttccataggctccgccc
                     c"
     primer_bind     8346..8365
                     /label=oRB_254_phPGK1-F1
                     /note="color: black; sequence: agagcagccgcgtccctgcg"
     primer_bind     complement(8383..8404)
                     /label=oJML0278 pPGK-LPmod_F
                     /note="color: black; sequence: 
                     CATGCGTCTCGTCGGTTGGGGTTGCGCCTTTTCCA"
     primer_bind     complement(8383..8402)
                     /label=oLM1202.PL2.v2F1
                     /note="color: black; sequence: 
                     ggtaatataacttcgtataggatactttatacgaagttatTTGGGGTTGCGCCTTTTCC
                     A"
     primer_bind     complement(8389..8406)
                     /label=oLM1178.PL2.F1
                     /note="color: black; sequence: 
                     AAGCCTTGCGACAGACCTCCCACTTAGATTGCCACGCATAGAGGGGTTGGGGTTGCGCC
                     T; added: 2020-09-28"
     primer_bind     complement(8407..8424)
                     /label=oJML0396
                     /note="color: black; sequence: 
                     GTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTCTCAGAGACGCCTGCAGG; 
                     added: 2020-09-28"
     primer_bind     join(8442..8458,1..14)
                     /label=oJML0169
                     /note="color: black; sequence: 
                     TACGCGTACGTGTTTGGGCCATGAAGCAACGCGCGCCTCTCAGGTGATAACTTCGTATA
                     G; added: 2020-09-28"
     primer_bind     join(8457..8458,1..31)
                     /label=oSP340
                     /note="color: black; sequence: 
                     cgaaggatctggaggccaccatggtgtcgataacttcgtatagcatacattatacgaag
                     t; added: 2020-09-28"
     primer_bind     complement(join(8458,1..33))
                     /label=oSP291_500bp2loxStuffer_EF1alpha_R
                     /note="color: black; sequence: 
                     taacttcgtataaagtatcctatacgaagttatCatggctagagacttatcgaaagcag
                     c; added: 2020-09-28"
     primer_bind     join(8458,1..26)
                     /label=oJML0395
                     /note="color: black; sequence: 
                     GTGTGCGGCCGCCATGATAACTTCGTATAGGATACTTTATAC; added: 
                     2020-09-28"
     primer_bind     join(8458,1..15)
                     /label=oJML0187
                     /note="color: black; sequence: CTCAGGTGATAACTTCGTATAGG; 
                     added: 2020-09-28"
ORIGIN
        1 ataacttcgt ataggatact ttatacgaag ttataccggt ggctccggtg cccgtcagtg
       61 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaag
      121 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      181 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      241 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      301 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      361 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      421 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      481 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      541 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      601 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
      661 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
      721 acgggggtag tctcaagctg gccggcctgc tctggtgcct agtctcgcgc cgccgtgtat
      781 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
      841 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
      901 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
      961 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1021 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1081 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1141 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1201 tccatttcag gtgtcgtgac ggccgatgac cgagtacaag cccacggtgc gcctcgccac
     1261 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     1321 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     1381 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     1441 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     1501 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     1561 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtgtcgcc
     1621 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggccgccga
     1681 gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc cgcaacctcc ccttctacga
     1741 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     1801 catgacccgc aagcccggtg ccgtcgacgg cagcggcgcc accaacttca gcctgctgaa
     1861 gcaggccggc gacgtggagg agaaccccgg gcccgaattc gcctgtagag gaggagctgg
     1921 gaatggccac cgtgcctcag ctacactctc tcgggttagc cctggaagtc tttacacatg
     1981 tagaacccgt acccataata tctgcatggt atctgacttt ttctacccaa atatgggagg
     2041 cgtggaaagc cacatttacc agctctctca gtgcctgatt gaaagagggc ataaggttat
     2101 aattgtcacc catgcttatg gaaatcgaaa aggcatccgt tacctcacca gtggcctcaa
     2161 agtctattac ttgcctctga aagtcatgta caaccagtct acagccacga ccctctttca
     2221 cagtctgcca ttgctcaggt acatatttgt tcgggagaga gtcacgataa tccattcaca
     2281 tagttctttt tctgctatgg cccatgatgc tctcttccac gccaagacaa tggggcttca
     2341 gacagtgttc acggaccatt ccctttttgg atttgctgat gtcagctcgg tgcttacaaa
     2401 caagctgcta accgtgtctc tttgtgatac aaaccacatc atttgtgtgt cttatactag
     2461 taaggaaaat actgtactaa gagcagcact gaatcctgaa atagtgtccg tcattcctaa
     2521 tgctgtagat cctactgact tcactccaga cccatttaga aggcatgata gtataactat
     2581 tgttgttgtc agcagacttg tttacagaaa agggatcgat ttgcttagtg gtataatacc
     2641 tgaactctgt cagaaatatc cagatttaaa tttcataatt ggaggagagg gaccaaagag
     2701 aatcattttg gaagaagttc gggaaagata ccagctgcat gacagggtgc gtcttttggg
     2761 agctttagaa cacaaggatg ttagaaatgt cttagttcaa ggacatattt ttctgaatac
     2821 ctcccttact gaagcattct gcatggccat cgtggaagca gccagttgtg gtttacaggt
     2881 tgtaagtacc agagttggtg ggattcctga ggtgcttcca gaaaacctta ttattttatg
     2941 tgagccttca gtaaaatctt tgtgtgaagg attggaaaag gctattttcc aactgaagtc
     3001 agggacattg ccagctccag aaaacatcca taacatagta aagactttct acacctggag
     3061 gaatgttgca gaaagaactg aaaaggtata tgaccgggta tcagtggaag ctgtgttgcc
     3121 aatggacaaa cgactggaca gacttatttc tcactgcggc ccagtaacag gctacatctt
     3181 tgctttgttg gcagttttca acttcctctt cctcattttc ttgagatgga tgactccaga
     3241 ttctatcatt gatgttgcaa tagatgccac tgggccacgg ggtgcctgga ctaataacta
     3301 ttctcacagt aaaagagggg gtgagaataa tgagatatct gaaaccaggg gatccggctc
     3361 aggcgccacc aactttagcc tgctgaagca ggcaggcgac gtcgaggaga accccggccc
     3421 tacgcgtatg gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca
     3481 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc
     3601 ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacca agcaccccgc
     3661 cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3721 gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac
     3781 cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca
     3841 gaagaaaaca atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct
     3901 gaagggcgac attaagatgg ccctgcgcct gaaggacgga ggccgctacc tggcggactt
     3961 caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgttgaccg
     4021 caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc
     4081 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaggta cctgaggcca
     4141 accttctatc agagttaaac ttttgacaag ggaacaaatc tcaaactgat ccatcagtca
     4201 tgtagctagc tgtagagctt gcaacttaat agcagcagct gcccaatgcc atgtgaagta
     4261 acaaactggt ttttggtttt tttttcccct tcagttttaa tgttatgtgt aatgtattta
     4321 aacccttatt taaataaaac ttgttttcag aaatacctga cttgcagatt atttatatat
     4381 agtcgatctt gtgcatataa aggaaccata gaaatgggca gtggaggatt cgtccacacc
     4441 cacagcttct caacgggcct gaatttggcc aagaaaaacc tttatgagct tgtccctctc
     4501 tggtcaactg gttttttgtt cgtttgtttt ttgagacaat aacttcgtat agcatacatt
     4561 atacgaagtt attatgcgag accttatacg cacggtctca atccgttcaa gagacgattt
     4621 cgggtccaca acgaccttga aaaactattt cgggagagac ggagtcactg ccaaccgaga
     4681 cggtcatagc tgtttcctgt gtgccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttac ccacagaatc
     4801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctctttc gttcatccat
     5701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5761 cagtgctgca ataataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
     5821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5941 cgttgttgcc atcgctacag gcatcgtggt atcacgctcg tcgtttggta tggcttcatt
     6001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgc gcaaaaaagc
     6061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgccg tgttatcact
     6121 catggttatg gcagcactac ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6661 attaacctat aaaaataggc gtatcacgag gccctttcgt tgtaaaacga cggccagtcg
     6721 aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc tggcaaaatc atgtataagg
     6781 tgaagggcat ccctgcaggt aagatacatt gatgagtttg gacaaaccac aactagaatg
     6841 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6901 ataagctgca ataaacaagt tttagttagc ctcccccatc tcccgggcaa acgtgcgcgc
     6961 caggtcgcat atcgtcggta tggagccggg ggtggtgacg tgggtctgga ccatcccgga
     7021 ggtaagttgc agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa
     7081 gacgtgcatg gaacggaggc gtttggccaa gacgtccaag gcccaggcaa acacgttata
     7141 caggtcgccg ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc
     7201 gatatggggt cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggcggt
     7261 ccccgaaagc tgtccccagt cctcccgcca cgacccgccg cattgcagat accgcaccgt
     7321 attggcaagt agcccgtaaa cgcggcgaat cgcagccagc atagccaggt ccagccgctc
     7381 gccggggcgc tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggccccaag
     7441 cacgatgttg gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg
     7501 gggggtcatg ctgcccataa ggtagcgcgc ggcggggtag cacaggaggg cggcgatggg
     7561 atggcggtcg aagatgaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc
     7621 cccgatatga ggagccagaa cggcgtcggt cacggcataa ggcatcccca ttgttatctg
     7681 ggcgcttgtc attaccaccg ccgcgtcccc ggcggatatc tcaccctggt cgaggcggtg
     7741 ttgtgtggtg tagatgttcg cgattgtctc ggaagccccc agcacccgcc agtaagtcat
     7801 cggctcgggt acgtagacga tatcgtcgcg cgaacccagg gccaccagca gttgcgtggt
     7861 ggtggttttc cccatcccgt ggggaccgtc tatataaacc cgcagtagcg tgggcatcct
     7921 ccaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc
     7981 gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc
     8041 cagccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt
     8101 cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt cagacgtgcg
     8161 gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc
     8221 ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg
     8281 cgaacggacg tgaagaatgt gcgaggccca gggtcggcgc cgctgcgttt cccggaacca
     8341 cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc
     8401 aacccccctg caggcgtctc tgagcgccct cgagacctta tacgcacggt ctcaaacg
//
