LOCUS       pET11a-N-DiB-RM         5965 bp ds-DNA     circular     11-MAR-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..5965
                     /label="source"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3..326
                     /label="N-DiB-RM"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3..326
                     /label="Translation 3-326"
     primer_bind     complement(377..395)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      391..438
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(613..641)
                     /label="tet promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(668..686)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        754..858
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             859..1719
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(1077..1096)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      1890..2478
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     2379..2398
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     2632..2649
                     /label="L4440"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2664..2806
                     /label="bom"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2732..2751)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     2892..2914
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(2908..3099)
                     /label="rop"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(4411..5493)
                     /label="lacI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     5455..5474
                     /label="LacI-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(5494..5571)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     5777..5796
                     /label="pBRrevBam"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        5880..5898
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     5880..5899
                     /label="T7"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    5899..5923
                     /label="lac operator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             5938..5960
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 atATGGGTCA TCaCCACCAC CATCACCTCG AATCAACCAG CTTGTACAAG AAATCCAGCT
       61 CCACGCCGCC GCGTGGCGTG ACCGTAGTAA ATAATTTCGA CTGCAAACGT TATCTCGGTA
      121 CCTGGTATGA GATTGCCCGT TTTGATCACC GCGCAGAACG TGGACTGGAA AAAGTCACCG
      181 CAACATACAG CCTGCGTGAT GACGGCGGCC TGAATGTCAT CTTCAAAGGC TATAACCCTG
      241 ACAGAGGAAT GTGGCAGCAG TACGTTGGGA AAGCGTACTT TACCGGCGCA CCAACTCGCG
      301 CTGCGCTGAA AGTGGCGTTC TTTTAAggat ccggctgcta acaaagcccg aaaggaagct
      361 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      421 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatcccgc aagaggcccg
      481 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
      541 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
      601 ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
      661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1081 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     1141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1381 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     1441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1561 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2641 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2701 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2761 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     2821 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2881 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2941 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     3001 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     3061 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     3121 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     3181 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     3241 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     3301 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     3361 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     3421 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     3481 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     3541 caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt
     3601 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     3661 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     3721 gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg
     3781 gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc
     3841 gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta
     3901 agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
     3961 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
     4021 gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg
     4081 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     4141 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     4201 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     4261 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     4321 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     4381 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4441 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4501 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     4561 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     4621 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     4681 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     4741 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     4801 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     4861 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4921 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4981 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     5041 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     5101 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     5161 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     5221 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     5281 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     5341 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     5401 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     5461 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     5521 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     5581 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     5641 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     5701 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     5761 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     5821 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     5881 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     5941 gtttaacttt aagaaggaga tatac
//