LOCUS       Exported                7130 bp ds-DNA     circular SYN 19-JUN-2009
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 10, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     /Takayuki/Ape/vectors/pCG.ape from 1 to 5615
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    11..651
                     /label=Ori
                     /note="/vntifkey=21"
     misc_feature    800..1659
                     /label=AMP (801-1660)
                     /label=AMP\(801-1660)
                     /note="/vntifkey=21"
     misc_feature    1810..2173
                     /label=CMV Enhancer (1811-2174)
                     /label=CMV\Enhancer\(1811-2174)
                     /note="/vntifkey=21"
     misc_feature    2174..2455
                     /label=Ch B-act (2175-2456)
                     /label=Ch\B-act\(2175-2456)
                     /note="/vntifkey=21"
     misc_feature    2468..3421
                     /label=Ch B-act Intron (2469-3422)
                     /label=Ch\B-act\Intron\(2469-3422)
                     /note="/vntifkey=21"
     misc_feature    3462..3481
                     /label=FP179 for primer
     misc_feature    3531..5078
                     /label=SRGAP1FBAR(516a.a.)
     misc_feature    4175
                     /label=GTA(V)to GTG(V) mutation
     misc_feature    5103..5822
                     /label=EGFP
     misc_feature    5148..5169
                     /label=pEGFP-N1_R1
     misc_feature    5148..5169
                     /label=pEGFP-N1 rev primer
     misc_feature    5834..6359
                     /label=rabbit beta-globin polyA signal
                     /label=rabbit\beta-globin\polyA\signal
                     /note="/vntifkey=21"
     misc_feature    5891..5910
                     /label=pCIG2R2
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagata atgtccaccc
     3541 cgagccgatt caagaaggac aaagagatca tagccgagta tgaaagtcaa gtcaaagaaa
     3601 ttcgagctca actggtagaa caacaaaaat gcctggagca gcaaacggag atgcgagttc
     3661 agcttctcca ggatctgcaa gatttcttcc gaaaaaaagc tgaaattgag acggaatatt
     3721 cccggaatct agagaagtta gcagaaaggt tcatggcaaa aacaagaagc actaaggatc
     3781 atcaacaata caagaaagac cagaacctgt tgtctccagt gaactgctgg tatttgctcc
     3841 tgaaccaagt aaggagagaa agcaaagacc atgcaacctt gagtgacatc tatctgaaca
     3901 atgtgattat gcggttcatg cagataagtg aggattctac caggatgttt aaaaagagca
     3961 aagagattgc attccaactt catgaggatt taatgaaggt tcttaatgag ctttatacgg
     4021 tgatgaaaac ataccatatg tatcatgcag agagcatcag tgcagagagc aagctgaaag
     4081 aggccgaaaa acaagaggaa aagcaaattg ggagatctgg tgatccagtc ttccatattc
     4141 gactagagga gagacatcaa cggcgaagct ctgtgaagaa aattgaaaaa atgaaagaaa
     4201 aaagacaagc aaaatattca gaaaataagc taaaatcaat taaggcacgg aacgaatatc
     4261 tcctaacact tgaagccacc aatgcctcag ttttcaagta ctatattcat gatctttctg
     4321 atttaattga ttgctgtgat cttggctacc atgcaagtct gaacagagcc ctaagaacat
     4381 atctgtctgc ggagtacaac cttgaaacct ccagacatga gggcttagac attattgaga
     4441 atgcagttga taatttagag cccaggagcg ataagcagag attcatggag atgtaccctg
     4501 ctgcgttctg tccaccaatg aagtttgagt ttcagtctca catgggtgat gaggtgtgcc
     4561 aggtcagtgc ccagcagcca gtccaggcag agctcatgct caggtaccaa cagttgcagt
     4621 cccgccttgc cacgctcaaa atcgagaatg aagaggttaa gaaaacgact gaagccacct
     4681 tgcagacgat acaagatatg gtcaccatcg aggactatga tgtttctgaa tgcttccagc
     4741 acagtcgttc cacagaatct gtgaagtcca ctgtctctga aacctacctg agtaaaccca
     4801 gcatcgccaa gagaagagcc aaccagcagg aaactgaaca gttctacttc atgaaactca
     4861 gagaatattt ggaaggcagt aatctcatca caaaacttca agccaaacat gacttgctgc
     4921 agaggaccct gggagaaggt catagagctg aatatatgac tacaaggcct ccaaatgttc
     4981 cccctaagcc ccagaaacac aggaagtcca ggccccgctc acagtataat actaagttgt
     5041 ttaatgggga tttggaaaca ttcgtcaagg actcaggagc ccgggatcca ccggtcgcca
     5101 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5161 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5221 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5281 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5341 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5401 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5461 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5521 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5581 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5641 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5701 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5761 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5821 aaagcggccg caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     5881 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     5941 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     6001 tgcaatagtg tgttggaatt ttttgtgtct atcactcgga aggacatatg ggagggcaaa
     6061 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     6121 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     6181 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     6241 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     6301 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     6361 cctcgacctg caccgtcgac cagctggtcg acggtgcacc gtcgaccagc ttggcgtaat
     6421 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6481 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6541 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     6601 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6721 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6781 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6841 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6901 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     6961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
//
