LOCUS       Exported                8184 bp ds-DNA     circular SYN 13-AUG-2009
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 10, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(803..1462)
                     /label=AmpR
     misc_feature    1810..3421
                     /label=CAG promoter
     misc_feature    3528..3554
                     /label=Flag tag
     misc_feature    3555..5531
                     /label=mouse Nuak1 cDNA
     misc_feature    5569..6153
                     /label=IRES
     misc_feature    6157..6876
                     /label=eGFP
     misc_feature    6157..6873
                     /label=eGFP
                     /label=eGFP(1)
     primer_bind     complement(7475..7495)
                     /label=M13-rev
     misc_binding    complement(7501..7523)
                     /label=LacO
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagatg gattacaagg
     3541 atgacgacga taagatggaa ggggcggcag tgtccgcggc aggggatggc cctgctgtag
     3601 agaccggctt gccgggctct ccgctggagg cggtggccgg ggcgactgca gccccagtgg
     3661 agcccaggaa gccgcacggg gtgaagcgac atcaccacaa acacaacttg aagcatcgct
     3721 atgagctgca agagaccctg ggcaaaggta cctacggcaa agtcaagaga gccaccgaga
     3781 ggttttcagg ccgagtggtt gctataaaat ccatccgtaa ggacaaaatt aaggacgagc
     3841 tagacatggt tcacatcaga cgagagattg agatcatgtc atccctcaac catcctcata
     3901 tcatcagtat ttatgaagtg tttgagaaca aagataagat tgtgatcatc atggaatatg
     3961 ccagcaaagg agagctgtat gattacatca gtgagcgcag gcgcctcagc gagagggaga
     4021 ccagacactt cttccggcag attgtgtctg ccgtgcatta ctgtcacaag aatggtgtgg
     4081 tccaccggga cttgaagctg gagaacatac tgcttgatga caactgcaac attaagattg
     4141 ctgactttgg gctctccaac ctgtaccaga aggacaagtt cttgcaaaca ttttgtggga
     4201 gcccactcta tgcgtcgccc gagattgtca acggcaggcc ttaccgaggg ccagaggtgg
     4261 acagctgggc cctgggtgtc ttgctttaca ctctcattta tggaacgatg cccttcgatg
     4321 gctttgatca caaaaacctc atccggcaga tcagcagcgg agagtaccgg gagcccacac
     4381 aaccctcaga tgctcgggga ctcatccggt ggatgctgat ggtgaatccc gatcgcagag
     4441 ccaccatcga ggacatcgcc aaccattggt gggtgaactg gggctacaag agcagcgtct
     4501 gtgactgtga tgcccttcct gactctgagt ctccactctt ggcacggatc atcgattggc
     4561 atcaccgttc cacggggctg caggctgagg ctgaagccaa gatgaagggt ctggccaaac
     4621 ccggggcctc tgaggttgtg ttggagcggc agcggtcact gaagaagtcc aagaaagaga
     4681 acgactttcc ccagtctggc caggactccg tacctgaaag cccatccaag ctgagttcca
     4741 agaggcccaa gggcatcctg aagaaacgga gcaacagcga acaccgctcc cacagcactg
     4801 gctttatcga aggcattgtg agccctgcct taccatcccc tttcaaaatg gagcaagatt
     4861 tgtgccggac tgccatcccc ctccccagct cccccgaggc agatatgtca gggaagctca
     4921 gcctcaaaca gtctgccacg atgcccaaga aaggcatctt gaaaaagacc cagcagagag
     4981 aatctggcta ctattcatcc ccagagcgca gcgagtcctc ggaactgctg gacagcaacg
     5041 atgtggtcat cagtggtggc ctctcgtccc ctcccccaga cccagccagg ggaacttccc
     5101 acagcctgtc ctgccggagg aagggcatct taaaacatag tagcaggtac tcagatggtg
     5161 gcacagaccc tgccctcacc cgccctgaaa tgcccacact ggaatccttg tcacctcccg
     5221 gtgtcccctc cgatggcatc tctcggagct acagtcgccc gtccagtatc atcagtgatg
     5281 acagtgtcct gtccagcgac tctttcgact tgttagagct tcaagagaac cggccagccc
     5341 gccagcgcat ccgcagctgc gtctctgctg aaaacttcct ccagcttcag gactttgaga
     5401 caccacacaa ccggccccgg ccccagtacc tgaagcggct ggcagacagt agcttctccc
     5461 ttctcactga tatggacgat gtgactcagg tgtataagaa agcgctggag atctgcagca
     5521 agctcaacta ggaattctgc agtcgacggt accgcgggcc cgggatccgc ccctctccct
     5581 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5641 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5701 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5761 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5821 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5881 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5941 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     6001 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
     6061 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     6121 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg
     6181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     6361 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6601 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6661 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6841 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcaattc actcctcagg
     6901 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     6961 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     7021 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     7081 gtctatcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     7141 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     7201 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     7261 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     7321 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     7381 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcaccgt cgaccagctg
     7441 gtcgacggtg caccgtcgac cagcttggcg taatcatggt catagctgtt tcctgtgtga
     7501 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7561 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7621 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     7681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     7861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7981 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8161 aagaacatgt gagcaaaagg ccag
//
