LOCUS       Exported                6614 bp ds-DNA     circular SYN 04-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 15, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    156..640
                     /label=hSyn
     sig_peptide     723..797
                     /label=ER signal
     centromere      804..2624
                     /label=iLACO1.1
     misc_feature    2631..2720
                     /label=GPI
     misc_feature    2735..3339
                     /label=WPRE
     polyA_signal    3359..3837
                     /label=hGH polyA
     misc_feature    3877..4017
                     /label=R-ITR
     rep_origin      4109..4415
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    4934..5791
                     /label=AmpR
     rep_origin      5942..6609
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cggacgaata
      661 acgcgtggat ccaccggtga gctcccgcgg cggccgctct agattagtct gcagatatca
      721 ccatgggaat ccaaggaggg tctgtcctgt tcgggctgct gctcgtcctg gctgtcttct
      781 gccattcagg tcatagcaga tctatgttct ctcccttggc agtggcccag gctcgacgct
      841 ataggtggag gattcaaacc gcgtgggatg ctggaacagt aggctacagt ctgttccaaa
      901 agtttacgga gcgggtaaag gaactgacgg atggacagct tgaggtccag ccttttccag
      961 cgggtgcagt cgtaggcacg tttgatatgt tcgacgcggt caagactgga gttttggacg
     1021 gaatgaatcc tttcacgctg ttttgggcag ggaggatgcc ggttacagct ttcctctcca
     1081 gctacgccct cgggttggac cgacctgacc agtgggaaac atggttttac tccctgggtg
     1141 gcctcgacaa cgcccgacga gcctttgccg agcaaggcct cttctacgtg ggaccagttc
     1201 agcacgacct caatacaatt cattcaagga agccgatccg gagatttgag gacttcaaag
     1261 gtgtcaagct ccgagtgccg ggcggcatga ttgcggaagt ttttgcggcc gcaggagctt
     1321 ccaccgtcct cttgcctggg ggggaggtct accccgctct ggaaagaggg gtaatagatt
     1381 ggagccacaa cgtctatatc atggccgaca agcagaggaa cggcatcaag gcgaacttcg
     1441 agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca
     1501 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagacca
     1561 aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1621 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagaatgg
     1681 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1741 acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctccggca
     1801 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1861 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1921 acgacttctt caagtcggcc atgcccgaag gctacatcca ggagcgcacc atcttcttca
     1981 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2041 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcataagc
     2101 tggagtacag ctttaacgat ggtggtgcgg ctgattttgt tgggcctgca gtaaactata
     2161 acttggggtt ccatcaggtg gcaaagtaca tcataatggg gcctccagag acgcctgcca
     2221 ttcatcaacc tgtagacctc atggacttca ccataaacct taatcggtgg cgcagccttc
     2281 caaagcctct gcaagaaaga ttcatcgcgg cggttcacga gtactcttgg attcactacg
     2341 caggcattca gaaggctaat ttggaggcgt ggccgaaata tcggcaggca ggtgttgagg
     2401 tcatccggct gagcaacgag gatgttagaa aattcaggcg actcgctatt cccatttggt
     2461 ttaagtgggc taagatggat aaatactcac gagaggcatt cgcgtcccaa ctggagtata
     2521 tgaaaggtat aggttacgtt acggacgagg aattgaaggg cttgagcctg ggctccacct
     2581 ccggctccgg caagcccggc tccggcgagg gctccaccaa gggcgaattc cttgaaaatg
     2641 gtgggacatc cttatcagag aaaacagttc ttctgctggt gactccattt ctggcagcag
     2701 cctggagcct tcatccctaa ggatcatcaa gcttatcgat aatcaacctc tggattacaa
     2761 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2821 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2881 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2941 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3001 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3061 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3121 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3181 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3241 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3301 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     3361 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3421 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3481 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3541 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3601 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3661 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3721 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3781 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3841 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3901 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3961 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4021 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4081 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     4441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4501 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4561 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4621 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4681 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4741 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4801 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4861 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4921 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4981 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5041 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5101 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5161 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5221 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5281 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5341 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5401 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5461 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5521 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5581 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5641 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5701 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5761 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5821 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5881 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5941 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6001 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6061 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6121 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6181 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6241 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6301 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6361 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6421 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6481 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6541 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6601 ttttgctcac atgt
//
