LOCUS       Exported                6481 bp ds-DNA     circular SYN 04-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 15, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /label=bla
                     /note="AmpR promoter"
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2459..2626
                     /label=ITR
                     /note="ITR"
     promoter        2657..2894
                     /label=CMV enhancer
                     /note="CMV enhancer"
     promoter        2901..3178
                     /label=chicken beta-actin promoter
                     /note="chicken beta-actin promoter"
     sig_peptide     3343..3417
                     /label=ER signal
     centromere      3424..5244
                     /label=iLACO1.1
     misc_feature    5251..5340
                     /label=GPI
     misc_feature    5342..5371
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    5383..5971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5854..5865)
                     /codon_start=1
                     /label=1
                     /note="Factor Xa site"
                     /translation="IEGR"
     polyA_signal    6014..6135
                     /label=SV40 poly
                     /note="SV40 polyadenylation signal"
     primer_bind     6079..6098
                     /label=SV40pA
                     /note="SV40pA"
     misc_feature    6259..6426
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
     3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     3301 cgggctgtaa ttagcgctac cggactcaga tctgatatca ccatgggaat ccaaggaggg
     3361 tctgtcctgt tcgggctgct gctcgtcctg gctgtcttct gccattcagg tcatagcaga
     3421 tctatgttct ctcccttggc agtggcccag gctcgacgct ataggtggag gattcaaacc
     3481 gcgtgggatg ctggaacagt aggctacagt ctgttccaaa agtttacgga gcgggtaaag
     3541 gaactgacgg atggacagct tgaggtccag ccttttccag cgggtgcagt cgtaggcacg
     3601 tttgatatgt tcgacgcggt caagactgga gttttggacg gaatgaatcc tttcacgctg
     3661 ttttgggcag ggaggatgcc ggttacagct ttcctctcca gctacgccct cgggttggac
     3721 cgacctgacc agtgggaaac atggttttac tccctgggtg gcctcgacaa cgcccgacga
     3781 gcctttgccg agcaaggcct cttctacgtg ggaccagttc agcacgacct caatacaatt
     3841 cattcaagga agccgatccg gagatttgag gacttcaaag gtgtcaagct ccgagtgccg
     3901 ggcggcatga ttgcggaagt ttttgcggcc gcaggagctt ccaccgtcct cttgcctggg
     3961 ggggaggtct accccgctct ggaaagaggg gtaatagatt ggagccacaa cgtctatatc
     4021 atggccgaca agcagaggaa cggcatcaag gcgaacttcg agatccgcca caacatcgag
     4081 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
     4141 gtgctgctgc ccgacaacca ctacctgagc acccagacca aactttcgaa agaccccaac
     4201 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4261 atggacgagc tgtacaaggg cggtaccgga gggagaatgg tgagcaaggg cgaggagctg
     4321 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4381 agcgtgtccg gcgagggtga gggcgatgcc acctccggca agctgaccct gaagttcatc
     4441 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4501 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtcggcc
     4561 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4621 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4681 atcgacttca aggaggacgg caacatcctg gggcataagc tggagtacag ctttaacgat
     4741 ggtggtgcgg ctgattttgt tgggcctgca gtaaactata acttggggtt ccatcaggtg
     4801 gcaaagtaca tcataatggg gcctccagag acgcctgcca ttcatcaacc tgtagacctc
     4861 atggacttca ccataaacct taatcggtgg cgcagccttc caaagcctct gcaagaaaga
     4921 ttcatcgcgg cggttcacga gtactcttgg attcactacg caggcattca gaaggctaat
     4981 ttggaggcgt ggccgaaata tcggcaggca ggtgttgagg tcatccggct gagcaacgag
     5041 gatgttagaa aattcaggcg actcgctatt cccatttggt ttaagtgggc taagatggat
     5101 aaatactcac gagaggcatt cgcgtcccaa ctggagtata tgaaaggtat aggttacgtt
     5161 acggacgagg aattgaaggg cttgagcctg ggctccacct ccggctccgg caagcccggc
     5221 tccggcgagg gctccaccaa gggcgaattc cttgaaaatg gtgggacatc cttatcagag
     5281 aaaacagttc ttctgctggt gactccattt ctggcagcag cctggagcct tcatccctaa
     5341 ggatcctcta gagtcgacct gcaggcatgc aagcttatcg ataatcaacc tctggattac
     5401 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5461 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5521 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5581 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5641 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5701 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5761 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5821 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5881 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5941 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg gcggccgctt
     6001 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6061 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6121 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6181 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     6241 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
     6301 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     6361 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     6421 gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6481 t
//
