LOCUS       Exported                6792 bp ds-DNA     circular SYN 02-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 15, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        162..842
                     /label=GFAP
     sig_peptide     901..975
                     /label=ER signal
     centromere      982..2802
                     /label=iLACO1.1
     misc_feature    2809..2898
                     /label=GPI
     misc_feature    2913..3517
                     /label=WPRE
     polyA_signal    3537..4015
                     /label=hGH polyA
     misc_feature    4055..4195
                     /label=R-ITR
     rep_origin      4287..4593
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5112..5969
                     /label=AmpR
     rep_origin      6120..6787
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgcagatc taacatatcc tggtgtggag
      181 taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg
      241 aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc
      301 agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt
      361 gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc
      421 attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc
      481 agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc
      541 tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg
      601 gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg
      661 ggagagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
      721 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
      781 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
      841 gcggggatcc accggtgagc tcccgcggcg gccgctctag attagtctgc agatatcacc
      901 atgggaatcc aaggagggtc tgtcctgttc gggctgctgc tcgtcctggc tgtcttctgc
      961 cattcaggtc atagcagatc tatgttctct cccttggcag tggcccaggc tcgacgctat
     1021 aggtggagga ttcaaaccgc gtgggatgct ggaacagtag gctacagtct gttccaaaag
     1081 tttacggagc gggtaaagga actgacggat ggacagcttg aggtccagcc ttttccagcg
     1141 ggtgcagtcg taggcacgtt tgatatgttc gacgcggtca agactggagt tttggacgga
     1201 atgaatcctt tcacgctgtt ttgggcaggg aggatgccgg ttacagcttt cctctccagc
     1261 tacgccctcg ggttggaccg acctgaccag tgggaaacat ggttttactc cctgggtggc
     1321 ctcgacaacg cccgacgagc ctttgccgag caaggcctct tctacgtggg accagttcag
     1381 cacgacctca atacaattca ttcaaggaag ccgatccgga gatttgagga cttcaaaggt
     1441 gtcaagctcc gagtgccggg cggcatgatt gcggaagttt ttgcggccgc aggagcttcc
     1501 accgtcctct tgcctggggg ggaggtctac cccgctctgg aaagaggggt aatagattgg
     1561 agccacaacg tctatatcat ggccgacaag cagaggaacg gcatcaaggc gaacttcgag
     1621 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
     1681 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagaccaaa
     1741 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1801 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagaatggtg
     1861 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1921 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctccggcaag
     1981 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2041 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2101 gacttcttca agtcggccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     2161 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2221 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcataagctg
     2281 gagtacagct ttaacgatgg tggtgcggct gattttgttg ggcctgcagt aaactataac
     2341 ttggggttcc atcaggtggc aaagtacatc ataatggggc ctccagagac gcctgccatt
     2401 catcaacctg tagacctcat ggacttcacc ataaacctta atcggtggcg cagccttcca
     2461 aagcctctgc aagaaagatt catcgcggcg gttcacgagt actcttggat tcactacgca
     2521 ggcattcaga aggctaattt ggaggcgtgg ccgaaatatc ggcaggcagg tgttgaggtc
     2581 atccggctga gcaacgagga tgttagaaaa ttcaggcgac tcgctattcc catttggttt
     2641 aagtgggcta agatggataa atactcacga gaggcattcg cgtcccaact ggagtatatg
     2701 aaaggtatag gttacgttac ggacgaggaa ttgaagggct tgagcctggg ctccacctcc
     2761 ggctccggca agcccggctc cggcgagggc tccaccaagg gcgaattcct tgaaaatggt
     2821 gggacatcct tatcagagaa aacagttctt ctgctggtga ctccatttct ggcagcagcc
     2881 tggagccttc atccctaagg atcatcaagc ttatcgataa tcaacctctg gattacaaaa
     2941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3061 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3121 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3181 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3241 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3301 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3361 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3421 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3481 ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg
     3541 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
     3601 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
     3661 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
     3721 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
     3781 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
     3841 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
     3901 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
     3961 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat
     4021 tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4081 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4141 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4201 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4261 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4321 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4381 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4441 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4501 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4561 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     4621 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4681 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4741 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4801 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4861 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4921 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4981 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5101 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5161 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5221 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5281 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5341 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5401 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5461 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5521 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5581 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5641 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5701 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5761 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5821 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5881 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5941 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6781 ttgctcacat gt
//
